• Journal of Embryo Transfer
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• v.32 no.3
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• pp.235-241
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• 2017

To preserve genetic materials, cryopreservation of the semen from live animals is the main technique to establish cryo-banking system which could be used for artificial insemination and embryo transfer. However, the population of Korean black goat (KBG) becomes to dwindle in number and is now faced genetic erosion by crossbreeding with non-native breeds in small KBG farms. In this study, simple freezing method was used to preserve frozen semen from KBG using spermatozoa of cauda epididymis (CE) and electro-stimulated semen (ES). The negative effects of seminal plasma on fresh sperm was confirmed using precipitation test of Triladyl egg yolk diluent and sperm viability after thawing was compared between CE and ES spermatozoa. When seminal plasma of fresh ES semen was washed with semen washing media (SWM), the rates of live sperm shown no significant difference between CE and ES spermatozoa before freezing. However, the survival rate of frozen/thawed CE sperm was higher than ES ($74.6{\pm}10.6%$ vs $53.8{\pm}5.2%$) with significant difference (p < 0.05). The results of longevity test on frozen/thawed sperm from CE showed healthier sperm than ES. Therefore, spermatozoa from CE could be used for cryo-banking system in KBG lines. The more studies are needed to increase survival rate of ES semen.

• Membrane Journal
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• v.8 no.2
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• pp.77-85
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• 1998

To treat effectively EDIR (electrodeposition ion removal) wastewater in terms of CO$_{Mn}$ 1,500~2,000 ppm generated from aluminum painting process, a RO (reverse osmosis) process was designed and installed to recover and reuse the concentrated solvent sent back to the electrocodeposition tank while the permeate reused as rinse water. A RO system in which three polyamide-spiral wound modules ($102\Phi \times 1,016L$ mm) connnected in series had been running to treat 20 m$^3$ in waste volume in 3 days batch operation at the condition of system recovery of 30 %, applied pressure 11.5 $kg_f/cm^2$ and room temperature. During 42 hours continuous operation leading to 5-fold decrease in waste volume, nearly constant permeation flux of 390 l/m$^2$-hr was maintained and the permeate with average CO$_{Mn}$, 300 ppm was obtained which could be used for washing the remaining paint solution in ion-exchange tower instead of demineralized water. Also COD$_{Mn}$ rejection as a function of running time was observed to be in the range of 78~87 % and the observed solvent rejections for ethyl cellusolve, buthyl cellusolve and n-butanol were 79 %, 87 % and 70 %, respectively.

• The Korean Journal of Blood Transfusion
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• v.29 no.3
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• pp.291-300
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• 2018

Background: The use of a functionally closed system for the glycerolization and deglycerolization of red blood cells (RBCs) allows for prolonged post-thaw storage for more than 24 hours. The aim of this study was to assess glycerolization and deglycerolization processing for RBCs using a high glycerol method in the automated, closed system provided by Haemonetics ACP 215. Methods: Thirty-five packed RBCs were glycerolized using the ACP 215 to a final concentration of 40% (wt/vol). The units were either frozen as such (n=30) or excess glycerol was removed (n=5) before freezing. After storage at $-80^{\circ}C$, the units were thawed, deglycerolized and resuspended in SAG-M. The frozen-thawed RBCs were stored at $4^{\circ}C$, and analyzed for their stability and in vitro quality. Results: No prefreeze excess glycerol removal units showed significantly less potassium leakage during post-thaw storage compared to the prefreeze excess glycerol removal units. All measurements of the stability and in vitro quality of thawed RBCs prepared from frozen RBCs without the prefreeze removal of excess glycerol during post-thaw storage at $4^{\circ}C$ for 7 days were acceptable to the American Blood Bank Association's standards and European standards. Conclusion: RBCs frozen without prefreeze removal of excess glycerol and the ACP 215 simplifies cryopreservation procedure and increases the stability of frozen-thawed RBCs. This increases the practical applicability of cryopreserved RBCs in blood transfusion practice.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.576-586
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• 2019

Airlines need to reduce their greenhouse gas (GHG) emissions because of the Paris Climate Agreement and ICAO CORSIA. This examined the degree of the strategic responses to which the airlines have made and the problems in the emission trading system (ETS). According to the analysis, the total amount of emission all the airlines made in the last three years was 116% more than the emission allowance imposed by the central government resulting in 10.7 billion KRW additional emission expense. Airlines would also face an increased carbon cost due to the implementation of ICAO CORSIA by purchasing an additional paid-in emission allowance in international routes. Although it is effective to retire the old aircraft early and induce the brand-new fuel-efficient aircraft to reduce GHG emissions, it is impractical in the short-term due to the tremendous amount of investment. To reduce the emission, airlines are washing engines, using ultra-light ULD and carts in the cabin, increasing the use of flaps and preventing the use of APU. On the other hand, these are very limited measures for reducing emissions according to the ICAO's mandatory emission target.

• The Journal of the Korea institute of electronic communication sciences
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• v.17 no.2
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• pp.309-316
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• 2022

Advances in technology have improved people's lives comfortably and have developed more easily, safely and simply. We usually turn on hot water to set the temperature of the water in the bathroom and gradually adjust the temperature to find the temperature we want with our skin. In this situation, I thought, "What if there is a device that can see the temperature of water with my eyes and help with the interior of the bathroom while including a safe system," and tried to create a system that values stability. For example, if a child accidentally changes the temperature of the water to high temperature while washing, he or she can get burned. And the biggest purpose is to secure better safety by adding LCDs and LEDs so that we can visually know the temperature before feeling it tactilely. As a result of the experiment, there was no error between the temperature detected by the water temperature sensor and the temperature displayed on the LCD, and no error occurred up to 27 cm in the distance measurement experiment using the ultrasonic sensor. There has been an error of about 2% since 28cm or older, but there is no significant inconvenience in using it within the category of faucets.

• The Korean Journal of Nuclear Medicine Technology
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• v.26 no.2
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• pp.15-19
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• 2022

Purpose [⁶⁸Ga]PSMA-11 is needed the high reproducibility, excellent radiochemical yield and purity. In term of radiation safety, the radiation exposure of operator for its production also should be considered. In this work, we performed a comparative study for the fully automated synthesis of [⁶⁸Ga]PSMA-11 between non-cassette type and cassette type. Materials and Methods Two different type of modules (TRACERlab FX N pro for non-cassette type and BIKBox for cassette type) were used for the automated production of [⁶⁸Ga]PSMA-11. According to the previously identified elution profile, Only 2.5 ml with high radioactivity was used for the reaction. After adjusting the pH of the reaction solution with HEPES buffer solution, the precursor was added and reacted with at 95 ℃ for 15 minutes. The reaction mixture was separated and purified using a C18 light cartridge. The product was eluted with 50% EtOH/saline solution and diluted with saline. It was completed by sterilizing filter. In the non-cassette type, the aforementioned process must be prepared directly. However, in the cassette method, synthesis was possible simply by installing a kit that was already completed. Results Both total [⁶⁸Ga]PSMA-11 production time were 25±3(non-cassette type) and 23±3 minutes(cassette type). The radiochemical yield of the non-cassette type(65.5±5.7%) was higher than that of the cassette type(61.6±4.8%) after sterilization filter. The non-cassette type took about 120 minutes of preparation time before synthesis due to washing of synthesizer and reagent preparation. However, since the cassette type does not require washing and reagent preparation, it took about 20 minutes to prepare before synthesis. Both type of synthesizer had a radiochemical high purity(>99%). Conclusion The non-cassette type production of [⁶⁸Ga]PSMA-11 showed higher radiochemical yield and lower cost than the cassette type. However, The cassette type has an advantage in terms of preparation time, convenience, and equipment maintenance.

• Korean Journal of Medicinal Crop Science
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• v.16 no.5
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• pp.320-325
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• 2008

Machilus thunbergii has been showed relation to antimicrobial activity on minimal inhibitoty concentration (MIC) and colony forming inhibitory activity (CFIA) test, so that can be used to food preservatives for green vegetable. Nanoparticles has been made of edible materials. 80% of the nanoparticles has been characterized by image analyser and electron microscopy, showing in the range under 300 nm diameter. The sprayed nanoparticles remained on the surface of chicon even after washing by dilution water, then activate biological activities for storage of chicon with storing and releasement system of extracts. Chicon treated nanoparticle has been kept fresh condition about 2 months longer than 3 weeks of the non-treated control. It can be tell that treatment with nanoparticle of M. thunbergii extracts extends storage time of chicon possibly by inhibition of ethylene production through efficiency control on cell breathing.

• Child Health Nursing Research
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• v.19 no.3
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• pp.159-167
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• 2013

Purpose: The purpose of this study is to find out preschool children's health status and their mothers' health management in the vulnerable classes. Methods: The assessment tool was developed, taken into consideration existing studies, materials produced by the customized visiting health care system, and review of visiting nurses of health centers and related experts. Data were collected January to February 2010 from 259 mothers by visiting nurses, and analyzed using SAS program for descriptive statistics. Results: Body weight less than 3 percentile was found for 5.0% children and over 97 percentile for 7.7%. Atopy was found in 17.8% children, no hand-washing after toileting and before meal in 30.9% and 36.7% respectively, no breakfast in 15.8%, and irregular meal in 32.0%. Sex education was made by 45.7% mothers, regular dental check by 56.6%, and hearing and eyesight test by 61.1% and 66.8% respectively. Home environment for upbringing is 34.3 in the scale of 41, and accident prevention 17.5 in the scale of 22. Conclusion: It is necessary to make an intervention on children's weight, personal sanitation and meal time in the vulnerable classes. Mothers need to be educated for appropriate health care, and home environments to improve upbringing and accident prevention.

• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.129-138
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• 1991

Tetrahymena pyriformis is a free-living ciliate protozoan in the freshwater system. Experiments were carried out to determine whether intraperitoneal administration of T. pyriformis (GL strain) to mice activates macrophages to be able to kill Toxoplasma gondii tachyzoites in vitro. Mice were also injected intraperitoneally with several synthetic activators; dimethyldioctadecylammonium bromide (DDA), dextran sulfate, complete Freund's adjutant (CFA) as well as Toxoplasma and Tetrehymena Iysates in order to activate mouse peritoneal macrophages. One week after the administration of activators, peritoneal cells were harvested and the adherent macrophages were challenged with Toxoplasma tachyzoites. Macrophage monolayers were then fixed with absolute methanol after washing, and stained with Giemsa solution. The percentage of the adherent cells infected and total number of organisms per 100 macrophages were calculated to make toxoplasma-cidal activity of macrophages according to the cultivation time. Peritoneal macrophages from mice administered with Tetrahymena exhibited significant protection against target parasites as compared with those treated with synthetic activators. Among non-biological synthetic activators, DDA was evaluated as an ellcellent activator.

• Journal of the Korean Applied Science and Technology
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• v.31 no.4
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• pp.584-594
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• 2014

To increase of surface hydrophilicity of polymeric thin films is an important approaching technique for introduction of self-cleaning and/or antifogging properties on the surfaces of those films. In general, hydrophilic surface can be produced by coating non ionic surfactants or by increasing surface energy. Various non-ionic surfactants, such as Tween, Span, and PEG-PPG block copolymers were selected for our experiments, because they are cheap and well soluble in toluene system as well as they contain several reactive hydroxy fuctional groups with coupling agents. Blending conditions influence the PET film surface hydrophilicities. However, the introduction of only these surfactants on the surface of PET films did not show the high durability of hydrophilic properties after washing with water. To improve the durability two types of coupling agents such as epoxide and diisocyanate were adopted. Contact angle of water on hydrophilically coated PET film surface with 6 wt% of isophrone diisocyanate(IPDI) containing coating solution was reached to $8.7^{\circ}$, which was an indirect evidence for very high surface hydrophilicity. A light(500 nm of wavelength) transmittance value of coated PET film was changed only from 87% to 85% with keeping a good transparent property. This film can be usable for self-cleaning film industries.