• Animal cells and systems
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• 제14권1호
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• pp.17-23
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• 2010

We isolated warm temperature acclimation-related protein 65-kDa (Wap65) cDNA from the liver of olive flounder and investigated the mRNA expression of Wap65 and HSP70 in olive flounder exposed to osmotic (17.5, 8.75, and 4 psu) and thermal stress (25 and $30^{\circ}C$). The mRNA expression of Wap65 and HSP70 was increased by thermal stress. The mRNA expression of HSP70 was also increased by osmotic stress, whereas no significant change in Wap65 expression was detected. These results indicate that Wap65 mRNA expression occurs specifically in response to increases in water temperature, but not in response to osmotic stress. Plasma cortisol levels were also increased by osmotic and thermal stress. We also utilized the stress hormone cortisol to examine whether Wap65 expression is thermal-stress-specific. Cortisol treatment increased HSP70 mRNA expression in vitro, but had no significant effect on Wap65 mRNA expression. Thus, thermal stress, but not osmotic stress, induces Wap65 expression.

• Fisheries and Aquatic Sciences
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• 제17권4호
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• pp.471-478
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• 2014

Genomic organization, including the structural characteristics of 5'-flanking regions of two 65-kDa protein (WAP65) isoform genes associated with warm temperature acclimation, were characterized and their transcriptional responses to immune challenges were examined in the intestine, kidney and spleen of the mud loach (Misgurnus mizolepis; Cypriniformes). Both mud loach Wap65 isoform genes displayed a 10-exon structure that is common to most teleostean Wap65 genes. The two mud loach Wap65 isoforms were predicted to possess various stress- and immune-related transcription factor binding sites in their regulatory regions; however, the predicted motif profiles differed between the two isoforms, and the inflammation-related transcription factor binding motifs, such as NF-${\kappa}B$ and CREBP sites, were more highlighted in the Wap65-2 isoform than the Wap65-1 isoform. The results of qRT-PCR indicated that experimental immune challenges using Edwardsiella tarda, lipopolysaccharide or polyI:C induced the Wap65-2 isoform more than Wap65-1 isoform, although modulation patterns in response to these challenges were tissue- and stimulant-dependent. This study confirms that functional diversification between the two mud loach Wap65 isoforms (i.e., closer involvement of Wap65-2 in the acute phase of inflammation and innate immunity) occurs at the mRNA level in multiple tissues, and suggests that such differential modulation patterns between the two isoforms are related to the different transcription factor binding profiles in their regulatory regions.

• Industrial Engineering and Management Systems
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• 제8권1호
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• pp.54-65
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• 2009

The existence of Internet revolutionized the computer and communications world. With the rising demand for accessing the Internet, the mobile technology has incorporated the use of Internet in mobile phones. Wireless Application Protocol (WAP) has combined two of the widest utilized technology today: mobile phones and Internet. This study aims to assess the existing WAP pages offered by the top service providers in the Philippines through usability testing experiments. The paper also aims to identify existing problems in WAP interface by focusing on the errors committed by the users. From the usability study, it was found out that common usability problems are wrong selection of links, unclear grouping of categories, wrong feedback, and redundant links. The type of service provider used is significant in determining the performance of users while experience in accessing WAP is insignificant.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2006년도 추계 종합학술대회 논문집
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• pp.65-69
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• 2006

이동통신망의 진화와 더불어 다기능 휴대폰을 이용한 신규 사업모델들이 개발되고 있다. 이동통신사업자는 다기능 휴대폰을 이용하여 기존의 음성서비스에서 WAP, ME 등의 모바일 브라우저와 WIPI, J2ME, BREW 등의 이동멀티미디어 플랫폼을 이용한 무선인터넷 서비스를 제공하고 있다. 카메라, MP3, MPEG, 3D 게임엔진, DMB 등의 멀티미디어 솔루션, 블루투스, IrDA, W-LAN, 등의 PAN 기능, GPS 연동을 통한 위치정보 등의 다기능 휴대폰이 개발됨에 따라 이를 기반으로 한 다양한 모바일 어플리케이션이 이동통신사업자가 사용하는 플랫폼으로 개발되고 있다. 콘텐츠 사업자는 하나의 모바일 어플리케이션을 이동통신사업자가 제공하는 모바일 플랫폼에 맞추어 새로이 개발해야 하는 문제점을 갖고 있다. 본 논문에서는 이기종 모바일 플랫폼 간 어플리케이션 상호 호환을 위해 대표적인 무선인터넷 플랫폼인 WIPI와 BREW에서 변환 솔루션을 제안한다. WIPI와 BREW의 특성을 분석하고 이를 기반으로 하여 WIPI에서 BREW, BREW에서 WIPI로 모바일 어플리케이션을 변환하는 솔루션을 제안한다.