• Animal cells and systems
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• v.14 no.1
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• pp.17-23
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• 2010

We isolated warm temperature acclimation-related protein 65-kDa (Wap65) cDNA from the liver of olive flounder and investigated the mRNA expression of Wap65 and HSP70 in olive flounder exposed to osmotic (17.5, 8.75, and 4 psu) and thermal stress (25 and $30^{\circ}C$). The mRNA expression of Wap65 and HSP70 was increased by thermal stress. The mRNA expression of HSP70 was also increased by osmotic stress, whereas no significant change in Wap65 expression was detected. These results indicate that Wap65 mRNA expression occurs specifically in response to increases in water temperature, but not in response to osmotic stress. Plasma cortisol levels were also increased by osmotic and thermal stress. We also utilized the stress hormone cortisol to examine whether Wap65 expression is thermal-stress-specific. Cortisol treatment increased HSP70 mRNA expression in vitro, but had no significant effect on Wap65 mRNA expression. Thus, thermal stress, but not osmotic stress, induces Wap65 expression.

• Fisheries and Aquatic Sciences
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• v.17 no.4
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• pp.471-478
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• 2014

Genomic organization, including the structural characteristics of 5'-flanking regions of two 65-kDa protein (WAP65) isoform genes associated with warm temperature acclimation, were characterized and their transcriptional responses to immune challenges were examined in the intestine, kidney and spleen of the mud loach (Misgurnus mizolepis; Cypriniformes). Both mud loach Wap65 isoform genes displayed a 10-exon structure that is common to most teleostean Wap65 genes. The two mud loach Wap65 isoforms were predicted to possess various stress- and immune-related transcription factor binding sites in their regulatory regions; however, the predicted motif profiles differed between the two isoforms, and the inflammation-related transcription factor binding motifs, such as NF-${\kappa}B$ and CREBP sites, were more highlighted in the Wap65-2 isoform than the Wap65-1 isoform. The results of qRT-PCR indicated that experimental immune challenges using Edwardsiella tarda, lipopolysaccharide or polyI:C induced the Wap65-2 isoform more than Wap65-1 isoform, although modulation patterns in response to these challenges were tissue- and stimulant-dependent. This study confirms that functional diversification between the two mud loach Wap65 isoforms (i.e., closer involvement of Wap65-2 in the acute phase of inflammation and innate immunity) occurs at the mRNA level in multiple tissues, and suggests that such differential modulation patterns between the two isoforms are related to the different transcription factor binding profiles in their regulatory regions.

• Industrial Engineering and Management Systems
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• v.8 no.1
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• pp.54-65
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• 2009

The existence of Internet revolutionized the computer and communications world. With the rising demand for accessing the Internet, the mobile technology has incorporated the use of Internet in mobile phones. Wireless Application Protocol (WAP) has combined two of the widest utilized technology today: mobile phones and Internet. This study aims to assess the existing WAP pages offered by the top service providers in the Philippines through usability testing experiments. The paper also aims to identify existing problems in WAP interface by focusing on the errors committed by the users. From the usability study, it was found out that common usability problems are wrong selection of links, unclear grouping of categories, wrong feedback, and redundant links. The type of service provider used is significant in determining the performance of users while experience in accessing WAP is insignificant.

• Proceedings of the Korea Contents Association Conference
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• 2006.11a
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• pp.65-69
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• 2006

Cellular network evolution and development of multi-function cellular phones introduce new mobile business mode. Cellular operators provide mobile internet services using the mobile browser such as ME and WAP and the mobile multimedia platform such as WIPI, J2ME, and BREW. New mobile applications are developed using mobile platforms provided by cellular operators as cellular phones having multimedia solutions such as camera, MP3, MPEG, 3D game engine, DMB, PAN such as bluetooth, IrDA, W-LAN, and location information using GPS are developed. Content providers have problems of redevelopment of a mobile application for different mobile platforms. In this paper, we propose a conversion solution for interoperability of applications developed on different mobile platforms of WIPI and BREW. We analyze APIs of WIPI and BREW and develop conversion solutions.