• The Journal of Korean Obstetrics and Gynecology
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• v.25 no.1
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• pp.34-46
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• 2012

Objectives: The object of this study was to observe the in vitro antibacterial effects of Wandae-tang extracts and combination of Wandae-tang extracts and Clindamycin against Gardnerella vaginalis ATCC14018. Methods: Antibacterial activities against Gardnerella vaginalis ATCC14018 of Wandae-tang extracts were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were also monitored at minimal inhibitory concentration(MIC) and $MIC{\times}2$ levels. The combination effects of Wandae-tang extracts with Clindamycin were observed by Checkerboard microtiter assay, and the effects of bacterial growth curve was treated with Wandae-tang extracts MIC+Clindamycin MIC, 1/2 MIC and 1/4 MIC, respectively. Results: MIC of Wandae-tang extracts and Clindamycin against Gardnerella vaginalis ATCC14018 were detected as $1.719{\pm}0.856$(0.782~3.125) $mg/m{\ell}$ and $0.010{\pm}0.006$ (0.004~0.016) ${\mu}g/m{\ell}$. In addition, Clindamycin and Wandae-tang extracts were also showed marked dosage-dependent inhibition of bacterial growth, and more dramatical inhibitions were detected in Clindamycin+Wandae-tang extracts MIC treatment. Fractional inhibitory concentration index in combination of Wandae-tang extracts and Clindamycin were detected as $0.294{\pm}0.052$(0.250~0.375) at Checkerboard microtiter assay. Conclusions: The results obtained in this study suggest that Wandae-tang extracts showed antibacterial effects against Gardnerella vaginalis ATCC14018, and they also showed dosage-dependent inhibitory effects on the bacterial growth. In addition, combination treatment of Wandae-tang extracts with Clindamycin showed more synergistically potent inhibitory effects on the growth of Gardnerella vaginalis.

• Journal of Haehwa Medicine
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• v.16 no.2
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• pp.235-239
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• 2007

Flour genitalis(대하) apply to secretions of vaginal canal exept bleeding. Fluor genitalis is met with everywhere at gynecology disease and is frequently broken out at least one third of women. The greater parts of flour genitalis are not critically ill. The greater parts of flour genitalis are continuous with simple infections of cervix, vagina and uterine tube. Nowadays women increase Bihuh formed flour genitalis(비허형 대하), of which the reasons are stress by vigorous social activity, malnutrition due to harsh diet. so this study has determined that WandaeTang(완대탕) would be effective for recente women flour genitalise which is Bihuh formed flour genitalis.

• The Journal of Korean Obstetrics and Gynecology
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• v.32 no.3
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• pp.32-56
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• 2019

Objectives: The purpose of this study was to investigate the in vitro anti-inflammatory, anti-pruritic and antimicrobial effects of the three herbal prescription (EST, SCT, WDT), which has been traditionally used for treating leukorrhea induced by various infections in the female genital tract. Methods: In this experiment, the anti-inflammatory effects were evaluated by Nitric oxide (NO), $Interlukine-1{\beta}$ ($IL-1{\beta}$), Interlukine-2 (IL-2), Interlukine-6 (IL-6), Tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), Prostaglandin $E_2$ ($PGE_2$), Leukotriene $B_4$ ($LTB_4$) production amount and Inducible nitric oxide synthase (iNOS), Nuclear factor kappa B ($NF-{\kappa}B$), Cyclooxygenase-2 (COX-2) gene expression levels in RAW264.7 cells. And the anti-pruritic effects were evaluated by Histamine, Acetylcholine (ACh), Acetylcholinesterase (AChE), Substance P production amount in Mast cell/9 (MC/9) and Pheochromocytoma 12 (PC12) cells. The anti-microbial effect was measured by inhibition zone diameter on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. Results: As a result of measuring anti-inflammatory efficacy, $IL-1{\beta}$, IL-2, IL-6, $TNF-{\alpha}$, $PGE_2$, and $LTB_4$ production amounts were significantly reduced in the EST, SCT, WDT extraction groups compared with the control group, and significantly decreased the amount of $NF-{\kappa}B$, iNOS, and COX-2 gene expression and the amount of Phospho-Inhibitor kappa B alpha ($p-I{\kappa}B-{\alpha}$)/Inhibitor kappa B alpha ($I{\kappa}B-{\alpha}$) and $NF-{\kappa}B$ p65 protein expression. In addition, As a result of measuring the anti-pruritic effect, the amounts of histamine, ACh and Substance P were significantly decreased, and AChE production was slightly decreased, but it's significance did not appear. Finally the anti-microbial effects of EST, SCT, WDT extraction groups against Pseudomonas aeruginosa, Candida albicans and Aspergillus niger was inhibited, however the growth of Escherichia coli and Staphylococcus aureus was not inhibited. Conclusions: These data suggest that EST, SCT, WDT can be used to treat patients with leukorrhea.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.2
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• pp.267-276
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• 2008

According to a treatment of internal disease in oriental medicine, we recognize that Lee Dong-won's Eumhwalon has a critical meaning. However, there is no well organized study about that so far even though medical treatments and prescriptions affected by Lee Dong-won's Eumhwalon have been used in clinical medicine. Hence I have studied the effect on a treatment of internal disease of after age affected by Lee Dong-won's Eumhwalon focused on medical treatments and prescriptions. For the comprehensive study on the effect of Lee Dong-won’s(李東垣) Eumwhalon(陰火論) on a treatment of internal disease for after age, I have examined, collected, and summarized the related descriptions from various books and papers in this subject such as <Naek-yung(內經)> which is the bible of oriental medicine. Followings are obtained as a result: Lee dong-won said Eumhwa(陰火) might be happened if there are abnormalities of the spleen and the stomach which exercise going up and coming down(升降). Lee dong-won offered Gamonjeyol(甘溫除熱) and Sengyangsanwha(升陽散火) as treatments of Eumhwa(陰火) Gamonjeyol(甘溫除熱) affected the treatment and the pathological recognition of Daekilon(大氣論) by Jang suk-sun(張錫純). Consequently there are some prescriptions such as Sengham-tang(升陷湯), Wandae-tang(完帶湯), Kerwonjern(擧元煎), and so on affected by Gamonjeyol(甘溫除熱). Sengyangsanwha(升陽散火) affected Yuklumanju(逆流挽舟) by Yu chang(喩昌) a lot. It is a meaning of Sengyangsanwha(升陽散火) that he used Insampaedok-san(人蔘敗毒散) to dysentery. As a representative prescription, there are Insampaedok-san(人蔘敗毒散), Kamisoyo-san(加味逍遙散), Baekchuljakyak-san(白朮芍藥散), and so on.