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Characterization of the Cell-Surface Barriers to Plasmid Transformation in Corynebacterium glutamicum

  • Jang, Ki-Hyo;Paul J. Chambers;Chun, Uck-Han;Margare L.Britz
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.294-301
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    • 2001
  • The effects of including glycine and isonicotinic acid hydrazide (INH) in the growth medium (Luria broth, LBG) on the subsequent lysozyme-imduced protoplast formation and transformation efficiency of Corynebacterium glutamicum were studied. The transformation efficiency of C. glutamicum AS019 increased up to 100-fold as the ocncentrationof glycine in the media increased from 0% to 5% (w/v), relative to cells grown in the absence of glycine. The presence of 5 mg/ml INH in the growth medium led to a further 10-fold increase in transformation efficiency. In addition, this transformation protocol was successfully applied to other strains of C. glutamicum. Both chemicals affected the mycolic acid attachment to the cell surface of C. glutamicum, when INH, the relative percentage of fatty acids of AS019 to the total lipids (mycolic acid plus fatty acids) decreased from 76.9% (in LBG) to 72.9% (in LBG-2% glycine) and 66.4% (in LBG-8 mg InG/ml), thereby suggeting that these chemicals also inhibit fatty acid synthesis.

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Increasing Dietary Phosphorus Level for Finishing Yearling Holstein Steers

  • Brokman, A.M.;Lehmkuhler, Jeff W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.2
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    • pp.220-224
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    • 2008
  • The need for refining dietary nutrient levels and limited information regarding Holstein steer and phosphorus supplementation led to the objective of examining the response of removing supplemental dietary phosphorus from a corn-based finishing diet offered to yearling Holstein steers. Two groups of yearling Holstein steers were utilized to study responses of increasing dietary phosphorus level during the finishing period. In Exp. 1, 96 Holstein steers (419 kg) were blocked into four weight groups. Dietary treatments included no additional phosphorus (NDC) or the inclusion of dicalcium phosphate (DCP) to achieve 0.30% phosphorus (P) in the complete diet. Daily gain, DMI and carcass traits were not different (p>0.05). Overall gain efficiency was slightly lower for NDC (p<0.05). Exp. 2 consisted of 78 Holstein steers (491 kg) blocked into two weight groups. Steers were harvested on d 84 and 112 d on test with carcass data collected following a 48-h chill. No differences (p>0.05) were detected for DMI, ADG, or gain efficiency. No differences (p>0.05) were detected in carcass characteristics in this experiment. Percent bone ash, calcium, P, and bending moment also did not differ among treatments (p>0.05). Removing supplemental phosphorus in the feedlot diet did not produce adverse effects on steer performance, carcass, or rib bone characteristics for yearling Holstein steers.

Application of Carbon Nanotubes in Displays

  • Feng, T.;Sun, Z.;Zhang, Z.J.;Lin, L.F.;Ding, Hui.;Chen, Y.W.;Pan, L.K.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.1529-1531
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    • 2008
  • Since the discovery over a decade ago, carbon nanotubes (CNTs) have been attracting considerable attentions both from scientists and engineers. Because of the excellent field emission properties, such as high aspect ratio, extremely small diameter, and high emission current, CNTs become a potential candidate as field emitter for field emission display (FED) and lighting (FEL) as backlight for LCD. Due to the exceptional physical properties, such as superior thermal and electrical conductivities, as well as high stiffness and strength, the CNT-based composites can be as light-weight heat-sink or thermal spreader materials used for power electronic devices, such as power LED for general illumination. The CNTs for above applications will be reviewed, and related materials and devices will be demonstrated in this paper.

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An Experimental Study on the Sustainable Performance of Concrete through the Quantitative Analysis of Carbon Dioxide Absorption (이산화탄소 흡수량 정량분석을 통한 콘크리트의 친환경성능에 관한 실험적 연구)

  • Choi, Jin Young;Lee, Han Seung;Kyung, Je Woon;Lee, Sang Hyeon;Yang, Nae Won
    • KIEAE Journal
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    • v.7 no.4
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    • pp.113-118
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    • 2007
  • From the construction material the cement and the concrete which will reach to 90% are used to construction. But the cement occurrence (from the whole industry 4.4% of carbon dioxide exhaust quantity) makes the carbon dioxide of manufacture hour and anti- the recognition which is an environment industry. The cement absorbs the carbon dioxide during life period of the life time. It calls carbonation. In this study in order to evaluate the carbon dioxide absorption of the cement test produced the mortar specimens which it follows in the W/C. And carbonatable material of mortar specimens (calcium hydroxide) the quantitly it measured, reference study it led and absorption of carbon dioxide quantity it produced. Finally two result comparisons leads and it is a fundamental study which does the test evaluation possibility and a propriety investigation of carbon dioxide absorption quantity in objective.

Strategies for Container Terminal Development and Operation for the Hub port in Northeast Asia (동북아 중심항만 구축을 위한 컨테이너 터미널의 개발 및 운영전략)

  • Lee, C.Y.;Yang, W.
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 1998.10a
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    • pp.271-285
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    • 1998
  • This paper aims to suggest the strategies for Korea port, especially Busan to develope into a viable transhipment center and/or Hub port as the articulation points between mainline and feeder nets. To tackle this goal, the authors clarify the requirements for Korea port which will be critical in determining its position as Hub through the careful analysis on competitiveness, environmental impact, port policy and capacity, transhipment traffic and inherent ablity to generate traffic. The results are summerized as follows. (1) Coping with increasing container volumes, new investment is sustainedly necessary to increase the capacity and inherent ability of container port and create economies of scale. Moreover, increasing port capacity will increase the potential for the Korea port's inclusion into mainline rather than feeder networks. (2) Considering an increasing awareness of need for customer-oriented option s and service quality rather than simply a reliance on infrastructure-led efficiency, privatization of one sort or another and corporation of port have been key strategies in achieving greater efficiency in Korea containerport.

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Characteristics of working pressure on the ZnO Thin films prepared by RF Magnetron Sputtering System (RF magnetron sputtering 법으로 제조한 ZnO 박막의 증착 압력에 따른 특성)

  • Kim, Jong-Wook;Hwang, Chang-Su;Kim, Hong-Bae
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2010.06a
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    • pp.387-387
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    • 2010
  • 최근 ZnO 박막은 투명 박막, 태양전지, LED 등으로의 응용을 위한 새로운 기능성 박막으로 활발히 연구되어 지고 있다. ZnO 기반의 투명 박막 트랜지스터는 상온에서 증착 가능하여 유리기판을 이용한 광학소자와 플라스틱 기판을 이용한 플럭서블 소자 같은 차세대 전자소자를 구현 할 수 있다. 본 연구에서는 RF Magnetron Sputtering System을 이용하여 coming 1737 유리기판 위에 ZnO 박막을 공정압력에 따라 증착하고, 투명 반도체에 적합한 활용을 위한 구조적, 광학적 분석을 실시하였다. 박막 증착 조건은 초기 압력 $1.0{\times}10^{-6}$Torr, RF 파워는 100W, Ar 유량은 100sccm, 그리고 증착온도는 상온이었다. 증착 압력은 $7.0{\times}10^{-3}$, $2.0{\times}10^{-2}$, $7.0{\times}10^{-2}$Torr로 변화시켰다. 표면 분석 (SEM, AFM) 결과 증착압력이 고진공으로 변화함에 따라 결정립들이 감소하였고 RMS roughness값이 낮아졌다. 그리고 XRD 분석을 통해 피크강도는 증가하고 FWHM은 감소함을 보이고 있는데 이는 결정성이 좋아짐을 나타낸다. 그리고 광학 투과도를 통해 가시광 영역에서의 높은 투과도(85% 이상)을 확인하였고, 고진공으로 변화함에 따라 밴드갭이 넓어지는 것을 확인하였다.

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Femtosecond laser induced shock generation and its application (펨토초 레이저 유발 shock 형성 및 그 응용)

  • Jeoung, Sae Chae;Lee, Heung Soon;Sidhu, M.S.;Moon, Heh-Young
    • Laser Solutions
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    • v.17 no.4
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    • pp.1-6
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    • 2014
  • Femtosecond laser induced shock generation in water and vitreous humor of enucleated porcine eyeball was investigated. When focusing the femtosecond laser into the liquid mediums, the acoustic waves with a frequency of about 15.6kHz could be observed by using wide-band microphone. The amplitude of the acoustic signals from water has attained a maximum under a laser power of about 5mW. Further increment of the power results in a decrement of the acoustic signals due to nonlinear optical process including filamentation of laser beam. We have further investigated the effect of femtosecond laser induced acoustic waves by applying the laser pulse into enucleated porcine eyeball. The comparative studies on both healthy and diseased eyeballs led us propose that the femtosecond laser pulses could be utilized as a novel tools for treatment of partially detached retina layers from their choroid structures.

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Comparison on Spray Characteristics of Diesel HEV Injectors for 3-different Driving Type (SI, PI, DPI) (3개 구동방식(SI, PI, DPI)별 디젤HEV용 인젝터의 분무 특성 비교)

  • Chung, M.C.;Sung, G.S.;Kim, S.M.;Lee, J.W.
    • Journal of ILASS-Korea
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    • v.19 no.1
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    • pp.9-14
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    • 2014
  • Performance of DI diesel engine with high-pressure fuel injection equipment is directly related to its emission characteristics and fuel consumption. So, the electro-hydraulic injector for the common-rail injection system should be designed to meet the precise high fuel delivery control capability. Currently, most high pressure injector in use has a needle driven by the solenoid coil energy or the piezo actuator controlled by charge-discharge of output pulse current. In this study, macroscopic spray approaching method was applied under constant volume chamber to research the performance of three different injectors : solenoid, indirect-acting piezo and direct-acting piezo type for CR direct-injection. LED back illumination for Mie scattering was applied on the liquid spray visible of direct-acting piezo injector, including hydraulic-servo type solenoid and piezo-driven injectors. As main results, we found that a direct-acting piezo injector had better a spray tip penetration than hydraulic-servo injectors in spray visualization.

The Effects of Welding Wires on the Weldabilities of API X-100 with Laser-Arc Hybrid Welidng (API X-100의 레이저-아크 하이브리드 용접성에 미치는 용접와이어의 영향)

  • Kim, Sungwook;Lee, Mok-Young
    • Journal of Welding and Joining
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    • v.32 no.5
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    • pp.7-12
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    • 2014
  • In this study, API-X100 steel pipes were welded with various kinds of welding wires in the laser-arc hybrid welding process. 10kW fiber laser source was combined to MIG arc welding process. API X-100 steel of base metal was of 16.9mm thickness, and butt welding applied. After welding, full penetration weld was acquired by 1-pass welding. A root porosity and the lack of fusion was observed in some welding conditions. By the mixing the melted wire, acicular ferrite, polygonal ferrite, pro-eutectoid, aligned side plate, and bainite structures were observed at the weld metal. From the observation of hybrid weld, unmixed zone had more Ni and Cr. The unmixed zone was a 1/3 area of the weld metal. As the mechanical test of the hybrid welding, tensile test and impact test applied. From the tensile test, all of the welding except SM70S was fractured at the base metal. The result of the impact test at -30 degree C led 60J~320J of the absorbed energy. The result of the low-absorbed energy might be from the coarse equiaxed structures of the weld metal.

Field Performance Test and Prediction of Power Consumption of a Centrifugal Chiller (현장에서 운전중인 터보냉동기의 성능 측정과 전력 소비량 예측)

  • Jang, Yeong-Su;Sin, Yeong-Gi;Kim, Yeong-Il;Baek, Yeong-Jin
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.25 no.12
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    • pp.1730-1738
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    • 2001
  • This paper presents an overview of testing and analyzing field performance of a centrifugal chiller which has a rated capacity of 200 RT(703 kW). Field data of a chiller installed in the cleanroom research building of KIST has been collected far performance analysis. The operating data included start-up, shut-down, and quasi-static state where cooling capacity and compressor power consumption varied cyclically. It was found that the steady-state thermodynamic model could be applied to relate the cooling capacity and COP under quasi-static conditions. The results led to finding the required cooling load pattern and a possible energy saving method. This study provides a method of evaluating performance of a large capacity centrifugal chiller in which field test is necessary.