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Effect of Total Mixed Ration with Fermented Feed on Ruminal In vitro Fermentation, Growth Performance and Blood Characteristics of Hanwoo Steers

  • Kim, S.H.;Alam, M.J.;Gu, M.J.;Park, K.W.;Jeon, C.O.;Ha, Jong-K.;Cho, K.K.;Lee, S.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.213-223
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    • 2012
  • In this study, two experiments were conducted to evaluate the total mixed ration with fermented feed (TMRF) and total mixed ration (TMR) by rumen in vitro fermentation and their effects on the growth performance and blood characteristics of Hanwoo steers. In experiment 1, three Hanwoo steers ($600{\pm}47$ kg), each permanently fitted with a ruminal cannula were used. In this experiment, three diets designated as T1, TMRF (18.4% fermented feed, tall fescue, mammoth wild rye forage and whole crop barley); T2, TMRF (17.7% fermented feed, rice straw and whole crop barley); and T3, TMR (rice straw, whole crop barley and probiotics, but no fermented feed), which were subjected to rumen in vitro fermentation for 48 h. The results demonstrated that DM disappearance rate gradually increased with advancing fermentation time, but T1 and T2 were higher than the T3 (p<0.05) from 3 h to 12 h, but insignificant (p>0.05) at 24 and 48 h. None of the specific VFAs were affected except for acetic and non volatile lactic acids, which were produced more in T2 than in T1 and T3 at 24 h and 48 h of incubation. A/P was lower in T1 and T2 than inT3 at 24 h (p<0.05) and 48 h (p>0.05) of incubation. These results confirmed that TMRF-related treatment shows a superior performance to that of TMR during the ruminal fermentation period. In experiment 2, the three diets in experiment 1 plus 1 more control diet (concentrates, probiotics and 2% rice straw of body weight) were fed to the 48 Hanwoo steers ($160{\pm}10$ kg) for a period of 168 d. The results demonstrated that the daily and total live weight gain and feed efficiency were higher (p<0.05) in the TMRF and TMR groups than in the control group. SGOT, SGPT and BUN (p<0.05) were reduced in TMRF relative to the control and TMR groups by 168 d which confirmed that TMRF shows better blood profiles than the TMR and control groups. Overall, these results appear to show that TMRF has better in vitro ruminal characteristics than those of TMR; growth performance and blood profiles were also found to be superior in TMRF than in the TMR and control groups. Thus, our findings suggest that TMRF-based feed supplies are favorable for Hanwoo cattle.

Effects of Hydrated Potato Starch on the Quality of Low-fat Ttoekgalbi (Korean Traditional Patty) Packaged in Modified Atmosphere Conditions during Storage

  • Muhlisin, Muhlisin;Kang, S.M.;Choi, W.H.;Lee, K.T.;Cheong, S.H.;Lee, S.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.5
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    • pp.725-732
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    • 2012
  • This study was carried out to investigate the effects of hydrated potato starch on the quality of low-fat ttoekgalbi (Korean traditional patty) packaged in modified atmosphere conditions during storage. The ttoekgalbi was prepared from 53.2% lean beef, 13.9% lean pork, 9.3% pork fat, and 23.6% other ingredients. Two low-fat ttoekgalbi treatments were prepared by substituting pork fat with hydrated potato starch; either by 50% fat replacement (50% FR) or 100% fat replacement (100% FR). Both 50% and 100% FR increased the moisture, crude protein, and decreased fat content, cooking loss, and hardness. For MAP studies, 200 g of ttoekgalbi were placed on the tray and filled with gas composed of 70% $O_2$: 30% $CO_2$ (70% $O_2$-MAP) and 30% $CO_2$: 70% $N_2$ (70% $N_2$-MAP), and were stored at $5^{\circ}C$ for 12 d. During the storage time, both 50% and 100% FR showed higher protein deterioration, while no differences were found in CIE $a^*$, CIE $L^*$, lipid oxidation, and bacterial counts in comparison to control. The ttoekgalbi with 70% $O_2$-MAP was more red, lighter in color, and showed higher TBARS values compared with 70% $N_2$-MAP. The meat with 70% $N_2$-MAP showed lower aerobic bacterial counts in control than those with 70% $O_2$-MAP. The lower anaerobic bacterial counts were observed only in 50% FR and 100% FR packed with 70% $N_2$-MAP in comparison with 70% $O_2$-MAP. In conclusion, the fat replacement with hydrated potato starch showed no negative effects on the quality of low fat ttoekgalbi during storage and 70% $N_2$-MAP was better than 70% $O_2$-MAP for low-fat ttoekgalbi packaging.

Study on the Prolactin Receptor 3 (PRLR3) Gene and the Retinol-binding Protein 4 (RBP4) Gene as Candidate Genes for Production Traits in Berkshire Pigs

  • Do, C.H.;Cho, B.W.;Lee, D.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.183-188
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    • 2012
  • To investigate the influence of the prolactin receptor 3 (PRLR3) gene and the retinol-binding protein 4 (RBP4) gene on the production traits of swine, genotyping was performed on 156 and 141 Berkshire pigs, respectively, that were carefully selected for economic traits. The frequencies of allele A in the PRLR3 locus and allele B in the RBP4 locus were 0.50 and 0.42, respectively. Neither locus was in the Hardy-Weinberg equilibrium. After a genotype was assigned to the individuals whose parents had the homozygous genotype, a statistical analysis was conducted for 291 pigs. The animals with the PRLR3 and RBP4 genotypes included 182 and 227 head, respectively. Even though the genotypic effects of PRLR3 (p<0.05) and RBP4 (p<0.01) had a significant influence on the pigs' back fat thickness, the interaction of both genes was not highly significant in terms of the back fat thickness (p = 0.1235). While the estimated epistasis effects of aaBB and aaBb decreased the back fat thickness and reduced the growth rate, the effects of AAbb and aabb increased the growth rate. Despite the insignificant difference in the PRLR genotypes in terms of the days to 90 kg and the average daily gain, the back fat thickness showed a significant difference (p<0.05), and the additive effect of allele A and the dominant effect of the hetero-genotype were -0.377 and 1.206 mm, respectively. The RBP4 genotypes had a very significant effect (p<0.01) on the back fat thickness, the days to 90 kg, and the average daily gain. The additive effects of allele B of the RBP4 locus on the back fat thickness, the days to 90 kg, and the average daily gain were 0.70 mm, -1.3 days and 6.2 g, respectively. Moreover, the dominant effects of the heterozygote for those traits were 0.63 mm, 9.9 days and -45.0 g, respectively. Allele A of the PRLR3 locus favorably influenced the back fat thickness, the days to 90 kg of the body weight, and the average daily gain and its dominant effect unfavorably influenced those traits. Allele B of RBP4 showed an incremental growth rate and back fat thickness, which could lower the lean meat percentage in the carcass. The RBP4 hetero-genotype negatively affected the pork production. These results strongly imply that the selection of allele A of PRLR3 and allele B of RBP4 would produce highly productive pigs in the Berkshire breed. Careful selection of allele B of RBP4 is required because of the increase in the back fat thickness.

Antioxidant Effect of Korean Red Ginseng Extract on Aqueous Linoleic Acid and LDL (수용성계의 Linoleic Acid와 LDL에 대한 한국산 홍삼의 산화방지효과)

  • Lee, J.W.;Lee, S.K.;Do, J.H.;Sung, H.S.;Lee, H.O.
    • Applied Biological Chemistry
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    • v.40 no.4
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    • pp.283-288
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    • 1997
  • The antioxidant activities of Korean Red Ginseng Extract(RGE) and ${\alpha}-tocopherol$, as reference compound, were tested with HPLC and fluorometry which measure the MDA after reacting an aqueous 1% linoleic acid buffer solution, and LDL(1 mg protein/ml) buffer solution with $H_2O_2{\;}and{\;}FeCl_2$. The generation of conjugated-diene in LDL(0.25 mg protein/ml) was also measured by spectrometry. MDA determination showed the antioxidant effect on linoleic acid oxidation with oxidation inhibition ratio of 71.8% and 76.1%, respectively, by addition of 1000 ppm RGE and 100 ppm ${\alpha}-tocopherol$. LDL(1 mg protein/ml) oxidation was inhibited by 25.2% and 21.2%, respectively, by addition of 200 ppm REG and 100 ppm ${\alpha}-tocopherol$. The generation of conjugated diene in LDL(0.25 mg protein/ml) was also inhibited by 44.2%, by addition of 50 ppm RGE.

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Quality Characteristics of Wheat Nuruks by Storage Conditions of Liquid Starters Using Rhizopus oryzae N174 (Rhizopus oryzae N174를 이용한 액체종국 저장조건에 따른 밀누룩의 품질특성)

  • Choi, Jeong-Sil;Jung, Seok-Tae;Choi, Ji-Ho;Choi, Han-Seok;Baek, Seong-Yeol;Yeo, Soo-Hwan
    • Microbiology and Biotechnology Letters
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    • v.40 no.4
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    • pp.319-324
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    • 2012
  • Production of liquid starters using wheat bran as a medium for Rhizopus oryzae N174 and the changes in their characteristics noted during storage were investigated in this study. The optimal culture conditions of the liquid starters were determined to be 5~15% (w/v) wheat bran and 48~72 hrs of incubation. The effects of liquid starters with different storage periods and temperatures (-18, 4, 10 and $25^{\circ}C$) on the quality of wheat nuruk were evaluated. According to the results of the pH, acidity, reducing sugar and enzyme activities, it was found that liquid starter using wheat bran preserved for one day, at any temperature, is the best method of storage for seed cultures for R. oryzae N174.

The Effect of $K^+$ on the Inotropic Action of Acontium Tuber (부자 "부타놀" 분획의 강심 작용에 대한 Potassium 이온의 효과)

  • Hong, S.A.;Kim, M.S.;Park, C.W.
    • The Korean Journal of Pharmacology
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    • v.13 no.2
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    • pp.11-17
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    • 1977
  • The effects of varing concentrations of extracellular potassium on the positive inotropic actions of Aconiti tuber butanol fraction and ouabain were studied on isolated left atrium of rat. The patterns of active tension increment, such as time to peak and total duration of tension development, was contrasted in Aconiti tuber butanol fraction to ouabain. Ouabain did not induce significant shortening in time to peak and total duration of tension development, but Aconiti tuber produce significant shortening. The isometric tension and peak dF/dt were not significantly different at the range of extracellular potassium concentration $2mEq{\sim}10mEq$ per. liter in control studies. The ouabain-induced increase in active tension and peak dF/dt at 2mEq. per. liter potassium concentration was significantly different from that of 6mEq or 10mEq. per. liter, but there was no difference between 6mEq and 10mEq. per. liter. but Aconiti tuber is not. In contrast to ouabain, Aconiti tuber butanol fraction did not show potassium dependent positive inotropic effect. It produced almost same increment of tension and peak dF/dt at all the concentration of extracellular potassium concentrations in this experiment.

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Identification of Internal Resistance of Microbial Fuel Cell by Electrochemical Technique and Its Effect on Voltage Change and Organic Matter Reduction Associated with Power Management System (전기화학적 기법에 의한 미생물연료전지 내부저항 특성 파악 및 전력관리시스템 연계 전압 변화와 유기물 저감에 미치는 영향)

  • Jang, Jae Kyung;Park, Hyemin;Kim, Taeyoung;Yang, Yoonseok;Yeo, Jeongjin;Kang, Sukwon;Paek, Yee;Kwon, Jin Kyung
    • Journal of Biomedical Engineering Research
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    • v.39 no.5
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    • pp.220-228
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    • 2018
  • The internal resistance of microbial fuel cell (MFC) using stainless steel skein for oxidizing electrode was investigated and the factors affecting the voltage generation were identified. We also investigated the effect of power management system (PMS) on the usability for MFC and the removal efficiency of organic pollutants. The performance of a stack microbial fuel cell connected with (PMS) or PMS+LED was analyzed by the voltage generation and organic matter reduction. The maximum power density of the unit cells was found to be $5.82W/m^3$ at $200{\Omega}$. The maximum current density was $47.53A/m^3$ without power overshoot even under $1{\Omega}$. The ohmic resistance ($R_s$) and the charge transfer resistance ($R_{ct}$) of the oxidation electrode using stainless steel skein electrode, were $0.56{\Omega}$ and $0.02{\Omega}$, respectively. However, the sum of internal resistance for reduction electrode using graphite felts loaded Pt/C catalyst was $6.64{\Omega}$. Also, in order to understand the internal resistance, the current interruption method was used by changing the external resistance as $50{\Omega}$, $300{\Omega}$, $5k{\Omega}$. It has been shown that the ohm resistance ($R_s$) decreased with the external resistance. In the case of a series-connected microbial fuel cell, the reversal phenomenon occurred even though two cells having the similar performance. However, the output of the PMS constantly remained for 20 hours even when voltage reversal occurred. Also the removal ability of organic pollutants (SCOD) was not reduced. As a result of this study, it was found that buffering effect for a certain period of time when the voltage reversal occurred during the operation of the microbial fuel cell did not have a serious effect on the energy loss or the operation of the microbial fuel cell.

Development of Software Correlator for KJJVC (한일공동VLBI상관기를 위한 소프트웨어 상관기의 개발)

  • Yeom, J.H.;Oh, S.J.;Roh, D.G.;Kang, Y.W.;Park, S.Y.;Lee, C.H.;Chung, H.S.
    • Journal of Astronomy and Space Sciences
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    • v.26 no.4
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    • pp.567-588
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    • 2009
  • Korea-Japan Joint VLBI Correlator (KJJVC) is being developed by collaborating KASI (Korea Astronomy and Space Science Institute), Korea, and NAOJ(National Observatory of Japan), Japan. In early 2010, KJJVC will work in normal operation. In this study, we developed the software correlator which is based on VCS (VLBI Correlation Subsystem) hardware specification as the core component of KJJVC. The main specification of software correlator is 8 Gbps, 8192 output channels, and 262,144-points FFT (Fast Fourier Transform) function same as VCS. And the functional algorithm which is same as specification of VCS and arithmetic register are adopted in this software correlator. To verify the performance of developed software correlator, the correlation experiments were carried out using the spectral line and continuum sources which were observed by VERA (VLBI Exploration of Radio Astrometry), NAOJ. And the experimental results were compared to the output of Mitaka FX correlator by referring spectrum shape, phase rate, and fringe detection and so on. Through the experimental results, we confirmed that the correlation results of software correlator are the same as Mitaka FX correlator and verified the effectiveness of it. In future, we expect that the developed software correlator will be the possible software correlator of KVN (Korean VLBI Network) with KJJVC by introducing the correlation post-processing and modifying the user interface as like GUI (Graphic User Interface).

On the Sequences of Dialogue Acts and the Dialogue Flows-w.r.t. the appointment scheduling dialogues (대화행위의 연쇄관계와 대화흐름에 대하여 -[일정협의 대화] 중심으로)

  • 박혜은;이민행
    • Korean Journal of Cognitive Science
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    • v.10 no.2
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    • pp.27-34
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    • 1999
  • The main purpose of this paper is to propose a general dialogue flow in 'the a appointment scheduling dialogues' in German using the concept of dialogue acts. A basic a assumption of this research is that dialogue acts contribute to the improvement of a translation system. They might be very useful to solve the problems that syntactic and semantic module could not resolve using contextual knowledge. The classification of the dialogue acts was conducted as a work of VERBMOBIL project and was based on real dialogues transcribed by experts. The real dialogues were analyzed in terms of the dialogue acts. We empirically analyzed the sequences of the dialogue acts not only in a series of dialogue turns but also in one dialogue turn. We attempted to analyZe the sequences in one dialogue turn additionally because the dialogue data used in this research showed some difference from the ones in other existing researches. By examining the sequences in dialogue acts. we proposed the dialogue flowchart in 'the a appointment scheduling dialogues' 'Based on the statistical analysis of the sequences of the most frequent dialogue acts. the dialogue flowcharts seem to represent' the a appointment scheduling dialogues' in general. A further research is required on c classification of dialogue acts which was a base for the analysis of dialogues. In order to e extract the most generalized model. we did not subcategorize each dialogue acts and used a limited number of items of dialogue acts. However. generally defined dialogue acts need to be defined more concretely and new dialogue acts for specific situations should be a added.

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A study on the Properties of $In_{l-x}Ga_{x}As$ Grown by the TGS Methods (TGS법으로 성장한 $In_{l-x}Ga_{x}As$의 특성에 관한 연구)

  • Lee, W.S.;Moon, D.C.;Kim, S.T.;Suh, Y.S.
    • Proceedings of the KIEE Conference
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    • 1988.11a
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    • pp.372-375
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    • 1988
  • The III-V ternary alloy semiconductor $In_{l-x}Ga_{x}As$ were grown by the temperature Gradient of $0.60{\leq}x{\leq}0.98$. The electrical properties were investigated by the Hall effect measurement with the Van der Pauw method in the temperature range of $90{\sim}300K$. $In_{l-x}Ga_{x}As$ were revealed n-type and the carrier concentration at 300K were in the range of $9.69{\times}10^{16}cm^{-3}{\sim}7.49{\times}10^{17}cm^{-3}$. The resistivity was increased and the carrier mobility was decreased with increasing the composition ratio. The optical energy gap determined by optical transmission were $20{\sim}30meV$ lower than theoretical valves on the basis of absorption in the conduction band tail and it was decreased with increasing the temperature by the Varshni rule. In the photoluminescence of undoped $In_{l-x}Ga_{x}As$ at 20K, the main emission was revealed by the radiative recombination of shallow donor(Si) to acceptor(Zn) and the peak energy was increased with increasing the composition, X. The diffusion depth of Zn increases proportionally with the square root of diffusion time, and the activation energy for the Zn diffusion into $In_{0.10}Ga_{0.90}As$ was 2.174eV and temperatures dependence of diffusion coefficient was D = 87.29 exp(-2.174/$K_{B}T$). The Zn diffusion p-n $In_{x}Ga_{x}As$ diode revealed the good rectfying characteristics and the diode factor $\beta{\approx}2$. The electroluminescence spectrum for the Zn-diffusion p-n $In_{0.10}Ga_{0.90}As$ diode was due to radiative recombation between the selectron trap level(${\sim}140meV$) and Zn acceptor level(${\sim}30meV$). The peak energy and FWHM of electroluminescence spectrum at 77K were 1.262eV and 81.0meV, respectively.

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