• Nuclear Engineering and Technology
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• v.54 no.5
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• pp.1674-1685
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• 2022

The ball valve is an important device in the pipeline transportation system of nuclear power plants. Its operational stability and safety directly affect the normal working of nuclear power plants. In this study, the transient numerical simulation of the opening and closing process of a ball valve was conducted on the basis of the flow interruption capability experiment of the ball valve by using the moving mesh method and inlet and outlet variable boundary conditions. The flow rate and pressure difference with time of the opening and closing process of the ball valve were studied. The internal flow characteristics of the ball valve under different relative openings were analyzed in conjunction with the typical back-step flow structure. Results show that the transient numerical results agree well with the experimental results. The internal flow characteristics of the ball valve are similar at the same opening during opening and closing process. At small opening, the spool and outlet channels easily form a back-step flow structure. The disappearance and generation of backflow vortices during opening and closing occur at 85% opening and 75% opening, respectively. With the decrease in opening degree, the difference in vortex core area in the flow channel of the ball valve spool in the opening and closing process gradually appears. The research results provide some reference value for the design and optimization of ball valves.

• Wind and Structures
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• v.38 no.1
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• pp.59-74
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• 2024

Large Eddy Simulation (LES) is used to explore the influence of vibration frequency and amplitude on the aerodynamic performance of a rectangular cylinder with an aspect ratio of B/D=5 (B: breadth; D: depth of cylinder) at a Reynolds number of 22,000 near resonance frequency. In smooth flow conditions, the research employs a sequence of three-dimensional simulations under forced vibration with diverse frequency ratios f_e / f_o = 0.8-1.2 (f_e : oscillation frequency; f_o : Strouhal frequency when the rectangular cylinder is stationary ) and oscillation amplitudes A_h/D = 0.05 - 0.3. The individual influences of f_e / f_o and A_h/D on the characteristics of integrated and distributed aerodynamic forces are the focal points of discussion. For the integrated aerodynamic force, particular emphasis is placed on the analysis of the dependence of velocity-proportional component C₁ and displacement-proportional component C₂ of unsteady aerodynamic force on amplitude and frequency ratio. Near the resonance frequency, the dependencies of C₁ and C₂ on amplitude are stronger than that of frequency ratio. For the distributed aerodynamic force, the increase in frequency and amplitude promotes the position of the main vortex core and reattachment to the leading edge in the streamwise direction. In the spanwise direction, vibration enhances the spanwise correlation of aerodynamic force to weaken the three-dimensional effect of the flow field, and a lower frequency ratio and larger amplitude amplify this effect.

• Journal of Genetic Medicine
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• v.4 no.1
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• pp.45-52
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• 2007

Purpose : A simple, rapid, and highly sensitive analytical method for Gb3 in plasma was developed without labor-ex tensive pre-treatment by electrospray ionization MS/ MS (ESI-MS/MS). Measurement of globotriaosy lceramide (Gb3, ceramide trihex oside) in plasma has clinical importance for monitoring after enzyme replacement therapy in Fabry disease patients. The disease is an X-linked lipid storage disorder that results from a deficiency of the enzyme ${\alpha}$-galactosidase A (${\alpha}$-Gal A). The lack of ${\alpha}$-Gal A causes an intracellular accumulation of glycosphingolipids, mainly Gb3. Methods : Only simple 50-fold dilution of plasma is necessary for the extraction and isolation of Gb3 in plasma. Gb3 in diluted plasma was dissolved in dioxane containing C17:0 Gb3 as an internal standard. After centrifugation it was directly injected and analyzed through guard column by in combination with multiple reaction monitoring mode of ESI-MS/MS. Results : Eight isoforms of Gb3 were completely resolved from plasma matrix. C16:0 Gb3 occupied 50% of total Gb3 as a major component in plasma. Linear relationship for Gb3 isoforms w as found in the range of 0.001-1.0 ${\mu}g$/mL. The limit of detection (S/N=3) was 0.001 ${\mu}g$/mL and limit of quantification was 0.01 ${\mu}g$/mL for C16:0 Gb3 with acceptable precision and accuracy. Correlation coefficient of calibration curves for 8 Gb3 isoforms ranged from 0.9678 to 0.9982. Conclusion : This quantitative method developed could be useful for rapid and sensitive 1st line Fabry disease screening, monitoring and/or diagnostic tool for Fabry disease.