• Title/Summary/Keyword: Viviparous

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Characterization of Carotenoid Biosynthetic Pathway Using Viviparous Mutant Embryos in Maize ( Zea mays L. )

  • Lee, Byung-Moo
    • Plant Resources
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    • v.1 no.1
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    • pp.33-37
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    • 1998
  • Carotenoid compounds in embryos of wild-type(WT) and viviparous mutants of maize(Zea mays L.) were analyzed using high performance liquid ehromatography (HPLC) with a photodiode array detector. Zeaxanthin accumulates in WT embryos as the major carotenoid. Phytoene accumulates in vp2 and vp5. Phytofluene in w3 and ${\xi}$-carotene in the vp9 mutant embryos. This indicates that the vp2 and vp5 mutants impair phytoene desaturase from 15-cis-phytoene to 15-cis-phytofluene. The w3 mutant has neither an isomerase from 15-cis-phytofluene to all-trans-phytofuene nor phytofluene desaturase from phytofluene to ${\xi}$-carotene. The vp9 mutant does not have the ${\xi}$-carotene desaturase from ${\xi}$-carotene to lycopene. Our analysis shows that the terminal carotenoid. ${\gamma}$-carotene(${\beta},{\Psi}$-carotene), accumulates in the vp7 mutant embryos. The ${\varepsilon}$-carotene(${\varepsilon},{\varepsilon}$-carotene), a product of ${\delta}$-carotene(${\varepsilon},{\Psi}$-carotene) in some plants, however, has not been found in maize embryos. The vp7 mutant impairs a cyclization step from ${\gamma}$-carotene to both ${\beta}$-carotene and ${\alpha}$-carotene. We suggest that monocyclic ${\gamma}$-carotene is the sole precursor of both bicyclic ${\beta}$-carotene(${\beta},{\beta}$-carotene) and ${\alpha}$-carotene(${\beta},{\varepsilon}$-carotene) in maize.

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Effects of Plant Growth Regulator Treatments During the Grain Filling Period on Seed and Viviparous Germination in Rice (벼 등숙기의 생장조절제처리가 종실 및 수발아에 미치는 영향)

  • Sa, Jong-Goo;Kim, Kee-Sik;Han, Se-Ki;Huh, Beom-Lyang;Kim, Kwang-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.3
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    • pp.262-269
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    • 1988
  • Experiments were conducted to find out the method of reducing viviparous germination of rice seeds using growth regulators during grain filling period. Among the recommended varieties, early Japonica type showed higher rate of viviparous germination, while late Japonica type and Tongil type varieties did not show viviparous germination in field condition. Grain showed high germination ability from 30 days of grain filling period in Daeseongbyeo of Japonica type and 35 days in Taebackbyeo of Tongil type. Growth regulators, NAA, CMH and Reglone were effective for reducing viviparous germination to the two rice varieties and three treatment stages in this experiment. NAA and CMH were most effective at 100 ppm and Reglone was at 0.4-0.6% concentration. Germination rates of rice seed were not different from non-treatment when NAA CMH were treated, while Reglone lowered germination rate. NAA and CMH had no effects on grain filling rate for both varieties, which showed high grain filling rate as the non-treatment, but Reglone results low grain filling rate. Grain color was not changes when NAA was applied, while CMH and Reglone made a lot of grain to dark brown as the concentration became higher. When grain were hulled, NAA had no difference with the non-treatment whereas CMH and Reglone had lower rate of perfect brown rice when they were applied at 20-30 days after heading.

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Glyphina betulae(Hemiptera, Aphididase, Thelaxine)New to the Far Eastern Asia from Mt. Baekdusan, North Korea (백두산에서 채집한 극동아시아 미기록종 Glyphina betulae(매미목, 진딧물과, 납작진딧물아과)의 보고)

  • Lee, Seunghwan;Jaroslav Holman;Jan Havelka
    • Animal Systematics, Evolution and Diversity
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    • v.17 no.2
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    • pp.179-184
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    • 2001
  • An aphid species, Glyphina betulae( Linnaeus, 1758), which has been known only in western Paleoarctic, is recognized from Mt. Baekdu-san, North Korea. Besides the descriptions and illustration for apterous viviparous female and alate viviparous female, brief biology, host plahts and distribution are discussed. This is the first record of the genus Glyphina in Korean Peninsula.

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A New Record of Sinomegoura citricola (van der Goot, 1917)(Sternorhyncha: Aphididae) from Korea (한국 미기록 진딧물 Sinomegoura citricola (van der Goot, 1917)(Sternorhyncha: Aphididae)의 보고)

  • Lee, Seunghwan;Kim, Dong-Hwan
    • Korean journal of applied entomology
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    • v.41 no.1
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    • pp.9-14
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    • 2002
  • Sinomegoura citricola (van der Goot, 1917) is recognized on Citrus unshiu Markovich (Rutaceae), Photinia glabra (Thunb.) Maxim (Rosaceae), and Cinnamomum lourelirii Nees (Lauraceae) from Jeju-do, Korea. Apterous viviparous female, alate viviparous female, and an intermediate morph between the apterous female and alate female are described. This is the first record of the genus Sinomegoura in Korea.

Changes of the Osmolarity in Embryonic Body Fluid and the Maternal Ovarian Tissue of the Viviparous Teleost, Ditrema temmincki, during the Gestation Period (태생 경골어류, 망상어(Ditrema temmincki)의 임신기 동안 체내자어의 체액삼투압 및 모체 난소조직상의 변화)

  • LEE Jung Sick;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.2
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    • pp.157-164
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    • 1996
  • Dry weight and body fluid osmolarity of embryo, and maternal ovarian fluid and the ovarian inner tissue of the viviparous teleost, Ditrema temmincki, changed considerably during the gestation period. After the complete absorption of the egg yolk, average dry weight of the embryo increased to 373.76 mg, and the range of total length (TL) was from 6.0 to 60.0 mm. Osmolarity of the embryonic body fluid was 796,8 mOsmol/kg with TL 64.0 mm right before parturition. Ovarian outer membrane started to swelling clearly after fertilization, and maximized in March. The swelling of ovigerous folds was maximized in late April. Expansion of blood vessels and increase of hemocytes reached to their maximum right before parturition. The results of this study indicated that these changes are related to the nutritional and environmental adaptation of both the embryo and the maternal body during the gestation period.

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OsDOR1, a novel glycine rich protein that regulates rice seed dormancy

  • Kim, Suyeon;Huh, Sun Mi;Han, Hay Ju;Cho, Mi Hyun;Lee, Gang Sub;Kim, Beom Gi;Kwon, Taek Yun;Yoon, In Sun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.90-90
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    • 2017
  • Regulation of seed dormancy is important in many grains to prevent pre-harvest sprouting. To identify and understand the gene related to seed dormancy regulation, we have screened for viviparous phenotypes of rice mutant lines generated by insertion of Ds transposon in a Korean Japonica cultivar (Dongjin) background. One of the mutants, which represented viviparous phenotype, was selected for further seed dormancy regulation studies and designated dor1. The dor1 mutant has single Ds insertion in the second exon of OsDor1 gene encoding glycine-rich protein. The seeds of dor1 mutant showed a higher germination potential and reduced abscisic acid (ABA) sensitivity compared to wild type Dongjin. Over-expression of Dor1 complements the viviparous phenotype of dor1 mutant, indicating that Dor1 function in seed dormancy regulation. Subcellular localization assay of Dor1-GFP fusion protein revealed that the OsDor1 protein mainly localized to membrane and the localization of OsDOR1 was influenced by presence of a giberelin (GA) receptor OsGID1. Further bimolecular fluorescence complementation (BiFC) analysis indicated that OsDOR1 interact with OsGID1. The combined results suggested that OsDOR1 regulates seed dormancy by interacting with OsGID1 in GA response. Additionally, expression of OsDOR1 partially complemented the cold sensitivity of Escherichia coli BX04 mutant lacking four cold shock proteins, indicating that OsDOR1 possessed RNA chaperone activity.

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Varietal Difference of Viviparity and Germination - Inhibition of Rice Hull Extracts (벼 수발아의 품종간 차이와 왕겨 추출물질의 발아억제효과)

  • Kim, Bong Ku;Lee, Dong Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.434-440
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    • 1996
  • This experiment were conducted to elucidate the damage by viviparity during grain filling on grain quality and hulling recovery and to understand the varietal differences of viviparity and the effect of extractive from rice hull of hardly viviparous varieties on seed germination and seedling growth. Ten rice cultivars, such as three indica, four japonica and three Tongil-type rices, were used in this study. The brown rice yield was decreased along with increasing the artificial viviparous treatment periods. At six days after treatment, brown rice yield of Daeseongbyeo was reduce 20% as compared with control. The ratio of head rice was 41.3% at six days after viviparous treatment with $25^{\circ}C$ temperature and over 95% relative humidity, while it was 99.5% in control. Varietal differences of viviparity in three rice groups were ranged from 1.1 to 5.2% in indica rices, 9.2 to 79.7% in japonica rices and 2.1 to 63.7% in Tongil-type rices. Methanol extractives from rice hull of IR36, Shinunbongbyeo and Jungwonbyeo inhibited significantly the germination of Deaseongbyeo.

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Properities and Effects of Utilizable Materials for Organic Farming in Rice (벼 재배시 유기농업 활용자재의 특성 및 효과)

  • Moon, Young-Hun;Oh, Dong-Hoon;Choi, Joung-Sik;Na, Jong-Seong;Han, Seong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.17 no.4
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    • pp.319-323
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    • 1998
  • This experiment was conducted to investigate the effects and properties of organic materials, the fermented fowl droppings composts and the top viviparous malleatuses, utilizable for rice farming. At the 35th day after transplanting, the growth of rice plants was good in the order of organic farming, combined farming, conventional farming, and control plot. But stem number and dry weight of rice plant in organic farming decreased a little compared to those in conventional farming at the 65th day after transplanting. Rice yield decreased by 7% in organic farming and by 4% in combined farming compared to those of conventional farming. Weeding effect by inoculation of 5kg or 10kg of the top viviparous malleatuses per 10a was similarly 96% at the immediately inoculation after transplanting, 98.6% at the 7th days inoculation after transplanting and 70% at the 15th days inoculation after transplanting. By inoculation of 5kg per 10a at the 7th day and the 15th day after transplanting, rice yields decreased by 2% and 5%, respectively, compared to those of the conventional farming method. From the above results, it seems that the organic farming and the combined farming are useful and the inoculation of the top viviparous malleatuses is effective for control of weeds as the environmental preservation farming.

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Varietal Difference in Enzyme Activities during Preharvest Germination of Rice (벼 수발아시 종실내 효소활성의 품종간 차이)

  • Ko Jong-Cheol;Kim Bo-kyeong;Lee Kyu-Seong;Choi Weon-Young;Choi Heh-Ran;Cho Eun-Ae;Yu Song-Joong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.6
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    • pp.378-383
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    • 2005
  • This study was conducted in order to exam­ine relationships between the viviparous germination ability and the antioxidative system in rice seeds and seedlings. Enzyme activities was compared among the four varieties with different viviparous germination ability from the early or mid-late maturity group. $\alpha$-Amylase activities correlated with the viviparous germination rate (VGRs) and $\alpha$-amylase activity in seeds of 40 days after heading (DAH) was highest in Dongjinbyeo among mid­late maturing varieties (MLMVs) and in Daeseongbyeo among EMVs. $\alpha$-Amylase activity in dry mature seeds was also higher in varieties with higher VGR. Glucose contents in viviparously germinating seeds and mature dry, seeds were higher in varieties with higher VGRs. Cat-alase activities did not correlate with the VGRs in both maturity groups. However, peroxidase activities in viviparously germinating seeds were higher in varieties with higher VGRs.