• 제목/요약/키워드: Vitamin D$_2$

검색결과 689건 처리시간 0.031초

골아세포의 IGF-I 유전자 발현 및 세포증식에 대한 1,25-dihydroxyvitamin $D_3$의 영향 (The Effects of 1,25- Dihydroxyvitamin $D_3$ on Expression of IGF-I Gene and Cellular Proliferation in MC3T3-E1 Cells)

  • 최희동;이재목;서조영
    • Journal of Periodontal and Implant Science
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    • 제30권1호
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    • pp.39-52
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    • 2000
  • Polypeptide growth factor belong to a class of potent biologic mediator which regulate cell differentiation, proliferation, migration and metabolism. 1,25-dihydroxyvitamin $D_3$ decrease cell proliferation, and stimulate alkaline phosphatase activity which express in osteoblast during cell differentiation period. IGF-I is known to stimulate cell proliferation and differentiation too. 1,25-dihydroxyvitamin $D_3$ is known to increase IGF-I binding sites and IGF binding protein which inhibite the effect of IGF. The purpose of this study is to evaluate potential role of IGF-I as mediator that control the action of 1,25-dihydroxyvitamin $D_3$. MC3T3-E1 cell were seeded $5{\times}10^5/ml$ at 100mm culture plate in ${\alpha}-MEM$ containing 10% fetal bovine serum. After 48 hour incubation period, medium were changed ${\alpha}-MEM$ containing 5% fetal bovine serum. After 24 hours, $10^{-9}M$ 1,25-dihydroxyvitamin $D_3$ added. Total mRNA was extracted at 0, 6, 24, 48, 72 hour. PRPCR method was programed for the detection of IGF-I mRNA. In the both groups of 1,25-dihydroxy vitamin $D_3$ treated and control, alternative splicing form of IGF-I, IGF-IA and IGF-IB were expressed. In the 1,25-dihydroxyvitamin $D_3$ treated group, IGF-I mRNA expression was matained until 24 hour, there after expression was decresed. MC3T3-E1 cell were seeded $2.5{\times}10^4/ml$ at 24well plate in ${\alpha}-MEM$ containing 10% fetal bovine serum. After 48 hour incubation period, medium were changed ${\alpha}-MEM$ containing 3% fetal bovine serum. After 24 hours, $10^{-9}M$ 1,25-dihydroxyvitamin $D_3$ and 10 ng/ml IGF-I were added separately or together. Cell were cultured for 1 and 3 days, $2{\mu}Ci/ml\;[^3H]$ -thymidine was added for the last 24h of culture of each days. ${[^3H]}$-thymidine incorporation in to DNA was measured and expressed counter per minute(CPM). DNA synthetic activity was significantly decreased by 1,25-dihydroxyvitamin $D_3$ both at 1 day and 3 day, and in the combination group of 1,25-dihydroxyvitamin $D_3$ and IGF-I, DNA synthetic activity was also decreased both at 1 day and 3 days. IGF-I did not affect the DNA synthetic activity compared to control group both at 1 day and 3 day. From the above results, 1,25-dihydroxyvitamin $D_3$ was potent inhibitor of cell proliferaton in MC3T3-E1 cells. It assumed that the effect of 1,25-dihydroxyvitamin $D_3$ on osteoblast proliferation may be mediated in part by decreased level of IGF-I.

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한국농촌주민(韓國農村住民)의 계절별(季節別) 식품섭취조사연구(食品攝取調査硏究) (A Study on Seasonal Variations of Food Consumption of Korean Farmers)

  • 박명윤
    • Journal of Nutrition and Health
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    • 제9권1호
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    • pp.43-50
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    • 1976
  • This is the report of a food consumption survey of 193 members of 30 farm families in three rural villages of Korea. From a total of 188 households of the three villages, namely Wolgok in Kyunggi Do Province, Wachon in Kangwon Do Province, and Sobong in Cholla Namdo Province, 30 households were chosen for the survey. Four consecutive seasonal surveys, beginning in the autumn, November 1974 and finishing with the summer, August 1975, were made and each survey covered three consecutive days. The Precise Weighing Method was used in evaluating the kinds of food and nutrient intakes of the subjects. This method entails the accurate weighing of all foods by the investigator just prior to their consumption by the subjects under investigation. This survey was male to determine the actual food eaten by the members of these farm families at each season of the year, and to note any special differences in the foods consumed. The results obtained are summarized as follows: Food Intake The traditional ordinary diet of Korean farmers consists mainly of rice and other cereals as staple foods, and simple subsidary foods. The nutritional shortcomings of rice as a staple food are evident in the subclinical status of nutritional deficiencies. The mean consumption of cereals and their products was very high and it was more than half of the daily total food intake throughout all the seasons. The consumption of animal food, fruits, and fat by the farmers was very low. Fruit intake during winter and spring was almost nil. One of the special foods in the Korean dietary pattern is Kim-chi, a variety of pickled and fermented vegetables. The mean intake of Kim-chi during autumn and winter was very much higher than the other seasons. Korean farmers have difficulties in balanced year-round supply of food due to seasonality of food production and lack of food preservation. As nutrition problems of Korean farmers are highly influenced by seasonal variations, the expansion of an appropriate food preservation and storage programme should be encouraged. Special consideration should be given to new methods of preservation, the types of foods preserved, and production and consumption of nutritionally valuable foods for the improvement of the nutritional status in rural communities. Nutrient Intake Average adult rates for calorie and protein in the three study areas were 0.80 and 0.87 respectively, with little variations by villages. The adult caloric intake in averaged 2,928 kcal for autumn, 2,662 for winter, 2,673 for spring, and 2,760 for summer. Cereals contributed most of the total calories. In terms of the recommended daily dietary allowances for the Korean given by the Korea FAO Association, the diets in this survey were adequate except in the winter and spring. The protein intake averaged from 76.4 grams in autumn to 83.4 grams in summer and was near the recommended allowance, but a relatively large proportion of the total protein came from cereals and their products. The fat intake was unsatisfactory, ranging from 19.2 grams to 29.3 grams for the four seasons, and the consumption during farming season was higher than other seasons. It is apparent that the diet of the Korean farmers should be supplemented by calcium during autumn and winter. The highest calcium intake, with a mean of 583.5 milligrams, was recorded in summer. The mean intake of iron ranged from a low of 9.2 milligrams in the autumn to a high of 15.0 milligrams in the spring and it was satisfactory for all seasons. Among the vitamin group, the daily average intakes of vitamin A, thiamine, riboflavin, and ascorbic acid were lower than the desired intake throughout all the seasons. The mean vitamin A intake as ${\beta}-carotene$ ranged from a low of 2,807.8 IU to a high of 5,221.0 IU. The thiamine intake ranged from 0.98 milligrams to 1.19 milligrams, while mean riboflavin intake ranged from 0.92 milligrams to 1.13 milligrams. Ascorbic acid intake was 37.3 milligrams, the lowest, and the highest 47.8 milligrams. Niacin intake among Korean farmers was higher than the recommended allowance throughout all the seasons, and the mean intake ranged from 18.3 milligrams to 25.8 milligrams. The main character of the Korean farmers' diet has been found low in quality of protein and high in carbohydrate. Attention should be paid to the low intake of vitamin A, thiamine, riboflavin, and ascorbic acid among Korean farmers.

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일부 학동기 어린이들의 비만도와 혈청지질 및 이에 영향을 미치는 인자에 관한 조사연구 (Obesity, Serum lipid and Related Eating Behaviors of School Children)

  • 손숙미;이중희
    • 대한지역사회영양학회지
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    • 제2권2호
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    • pp.141-150
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    • 1997
  • This study was designed to investigate the obesity rate with anthropometric indices, nutritional status and serum lipid levels of 260 school children from 5th grade of elementry school residing in Puchon city. Obesity was defined as fat percentage that exceeds 25$\%$. The prevalence rate of obesity were 15.2$\%$ for male and 14.1$\%$ for female. Mean fat percentage, BMI and Rohrer indice of obese male were 30.3$\%$, 21.0 and 150.2 respectively and 28.0$\%$, 21.5 and 151.9 for obese female. Mean serum TG, apo-B of obese female was significantly lower than that of non-obese. Obese male did not show any difference in serum lipid levels. The proportion of students whose serum cholesterol levels exceed 170mg/dI, which is cutoff point of bordeline for coronary artery disease, was 12.0% for male and 15.6$\%$ for female. The major factors affecting nutritional status were the amount of living expense, existence of mother's job and the regularity of having breakfast. Children who's living expense more than 500 thousants Won showed lower intake of Iron, vitamin A, thiamin, niacin and ascorbic acid(p$\ll$0.05). Children who's mothers are having a job were observed with decreased height(p$\ll$0.05). Children having breakfast regularly showed increased BMI and Rohrer indice than those of children having breakfast often of never.

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목질진흙버섯균 Phellinus linteus의 균사체 생육에 미치는 주요 인자에 관한 연구 (Studies on the Main Factors Affecting the Mycelial Growth of Phellinus linteus)

  • 지정현;하태문;김영호;노영덕
    • 한국균학회지
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    • 제24권3호통권78호
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    • pp.214-222
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    • 1996
  • 목질진흙버섯(상황버섯)은 약용버섯으로써 강력한 항종양 효과를 나타내는 것으로 잘 알려져 있으나, 자실체형성 및 발육에 관한 인공재배는 이루어지지 않고 있으므로, 본 연구는 목질진흙버섯균의 균사체 생육에 미치는 주요 인자에 대한 배양적 특성을 구명하여 인공재배 검토를 위한 기초자료를 얻기 위해 수행한 결과이다. 1. 균주의 균사배양에 적합한 온도는 $25{\sim}30^{\circ}C$, pH는 $6.0{\sim}7.0$의 범위였고, 균사생장 및 증식에 적합한 배지는 MCM, YM 및 Synthetic I 배지였다. 2. 균사생장이 양호한 탄소원으로는 단당류인 D-Glucose, D-Mannose, Dextrose였고, 질소원으로는 유기태질소원인 Cassamino acid와 아미노산류인 Glutamic acid, 복합질소원으로는 Peptone이 양호하였다. 3. 균사생장에 적합한 배지의 C/N비는 탄소원(D-Glucose)농도 2%에서 20:1이었고, 탄소원 농도가 높아질수록 균사생장은 부진하여 탄소원 농도 4% 이내가 적당하였다. 4. 유기산으로는 Gallic acid와 Silicic acid를 첨가하였을 때, 비타민으로 Biotin을 첨가하였을 때 균사생장이 촉진되었고, 무기염류는 비교적 다양하여 $KH_2PO_4,\;FeSO_4{\cdot}7H_2O,\;MgSO_4{\cdot}7H_2O,\;ZnSO_4{\cdot}7H_2O$의 최적농도가 0.05%, 0.001%, 0.02%, 0.0003%일 때 균사생장이 양호하였다.

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일부 학령기 아동의 구리 섭취량 및 구리 영양 상태에 관한 연구: 충남 벽지농촌과 도시간의 비교 (Dietary Copper Intakes and Nutritional Status of Copper in Serum among Elementary Schoolchildren in Chungnam Province in Korea: Comparison between Remote Rural and Urban Areas)

  • 김선효
    • Journal of Nutrition and Health
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    • 제39권4호
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    • pp.381-391
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    • 2006
  • This study is performed to compare the dietary intakes and food sources of copper (Cu) using the database of Cu content developed in this study between the elementary schoolchildren in remote rural areas (RA, n=58, $9.9{\pm}1.7$ yrs) and those in urban area (UA, n=60, $9.4{\pm}1.8$ yrs), and to analyze the relationship between serum Cu concentration and serum antioxidant status in the RA. The results obtained in this study were as followings: 1) Dietary intakes of calorie, calcium and iron in the RA were in the 3/5-4/5 of the Korean RDA while the UA were similar to or more than the Korean RDA, 7th ed. except iron. 2) More than 273 kinds of food consumed by the subjects were analyzed the content of Cu and database of Cu content were developed in the present study. The mean dietary intake of Cu per day in the RA was $0.99{\pm}0.07mg/d$ ($170.0{\pm}13.2%$ of the USA RDA) while it was $1.22{\pm}0.07mg/d$ ($203.4{\pm}13.1%$ of the RDA) in the UA. The percentage of dietary intakes of Cu less than 213 of the RDA was 8.6% in the RA in comparison to 0% in the UA. 3) The RA and the UA consumed more than 80% of total dietary intakes of Cu from plant foods. Thus, the RA and the UA consumed Cu from cooked rice, vegetables and fruits as a major source. However the RA had less Cu from meat and their products than did the UA (p<0.05) .4) Crab stew including crab and juice was the highest food source of Cu for the total subjects, followed by seasoned bud of aralia, cooked; beef rib meat, roasted; soybean paste soup w/mallow; and soybean paste soup w/mallow & beef. Major food source of Cu was similar for the RA and the UA such as cooked rice, vegetables and fruits. 5) Mean concentration of serum Cu in the RA was $18.1{\pm}0.7{\mu}M/L$ that was in the normal value, and all subjects in this group were in more than normal value. In the RA serum Cu concentration related positively with serum ceruloplasmin concentration, serum vitamin C concentration and EC SOD activity, respectively. However, serum Cu concentration did not relate with serum TBARS concentration in the RA. Above results showed that the RA had good status of Cu nutrition based upon dietary intake and serum concentration, however some of the RA had lower intake of Cu than the RDA. The overall children in the UA had good Cu nutrition. Therefore, the subgroup of the RA should be supported to improve their Cu nutrition, and this support could give them better antioxidant status based upon positive relationship between serum Cu concentration and serum antioxidant status in the RA.

폐경기 여성의 항산화 비타민 영양 상태와 혈중 지질 및 MDA 농도와의 관계 (Correlation of the Nutritional Status of Antioxidant Vitamins and Serum Lipids and MDA Levels in Postmenopausal Women)

  • 김상연;정경아
    • 동아시아식생활학회지
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    • 제16권2호
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    • pp.145-155
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    • 2006
  • This study investigated the correlation of the nutritional status of antioxidant vitamins and serum lipids and MDA levels in postmenopausal women. Data about general characteristics, dietary intakes and biochemical parameters, including serum lipids, MDA and antioxidant vitamins levels, were collected from 85 postmenopausal women. The subjects were classified into three groups according to their serum total cholesterol level: normocholesterolemia group (NC, < 200 mg/dL), moderate hypercholesterolemia group (MC, $200{\sim}239mg/dL$) and hypercholesterolemia group(HC, ${\geq}240mg/dL$). The results are as follows. 1) General characteristics and serum MDA levels were not significantly different among the three groups. 2) Daily nutrients intakes adjusted to energy intake were not significantly different among the three groups, and were compatible with dietary reference intakes (DRIs) for Koreans. 3) Dietary Vt. A, ${\beta}-carotene$, Vt. C and Vt. E intake were not significantly different among the groups, while Vt. E intake was positively related with serum TC (r=0.288, p<0.05) and triglyceride (r=0.341, p<0.001) levels. 4) Serum Vt. A level standardized by serum TC level was significantly low and serum Vt. E level was significantly high in the HC group. Serum Vt. E level was positively related with serum TC level (r=0.389, p<0.001). 5) Dietary Vt. E intake was negatively correlated to serum MDA level (r=-0.242 p<0.05). Serum Vt. C and Vt. E levels were also negatively correlated to serum MDA level (r=-0.312, p<0.001 and r=-0.299, p<0.05). When the correlation was analyzed only in the group with hypercholesterolemia, correlation coefficients between the antioxidant vitamin and serum MDA level were higher. We concluded that intakes of antioxidant vitamins can contribute to decreasing the risk of cardiovascular disease by decreasing the oxidative stress of body rather than by controlling serum lipid levels.

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강황지하부 부산물에서 분리한 Quercetin-3-O-${\beta}$-D-glucopyranoside-7-O-${\alpha}$-L-rhamnopyranoside가 선충의 수명연장에 미치는 영향 (Lifespan Extension Property of Quercetin-3-O-${\beta}$-D-glucopyranoside-7-O-${\alpha}$-L-rhamnopyranoside from Curcuma longa L. In Caenorhabditis elegans)

  • 안달래;이은별;김반지;이소연;안민실;은재순;신태용;김대근
    • 생약학회지
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    • 제45권4호
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    • pp.275-281
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    • 2014
  • After harvesting the medicinal parts of Curcuma longa, the remaining underground parts were discarded. From the remaining underground parts of Curcuma longa quercetin-3-O-${\beta}$-D-glucopyranoside-7-O-${\alpha}$-L-rhamnopyranoside (Q37) was isolated. The antioxidant activities in vitro and lifespan-extension effect of Q37 were elucidated using the Caenorhabditis elegans. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect of Q37 showed similar potent activities in comparison with vitamin C. Q37 also showed potent superoxide quenching activities as measured by the riboflavin- and xanthine-originated superoxide quenching activity tests. Q37 prolonged lifespan of worms under normal culture condition. In terms of protective effect of Q37 on the stress conditions such as thermal and oxidative stresses, Q37-treated worms exhibited enhanced survival rate, as compared to control worms. To know the possible mechanism of Q37-mediated increased lifespan and stress resistance of worms, we examined the activities of Q37on superoxide dismutase (SOD), and invested intracellular reactive oxygen species (ROS) levels. The results revealed that Q37 was able to elevate SOD activity of worms and reduce intracellular ROS accumulation in a dose-dependent manner.

충남 공주지역 대학생의 식사행동 및 액체섭취실태 조사 (A Survey on Dietary Behaviors and Liquid Consumptions of University Students in Kongju of Chungnam Province in Korea)

  • 김선효
    • Journal of Nutrition and Health
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    • 제42권4호
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    • pp.327-337
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    • 2009
  • University students tend to have various dietary problems including undesirable dietary behaviors, inadequate intakes of nutrients and biased habits of liquid consumption. This study was conducted to find dietary behaviors (n = 357) by questionnaire survey and to examine dietary nutrient intakes (n = 60) and liquid consumption (n = 853) by food record method for 3-days among university students attended in K University of Chungnam province in Korea. Most subjects lived in the dormitory or self-boarding house, and they skipped breakfast most frequently and took breakfast most irregularly among three meals. Dietary mean intakes of energy, Ca, vitamin B2 and folate were lower than the KDRI (37-85%), and those of males were poorer than those of females. Ratios of energy intake among three meals and snacks were not distributed evenly, so the mean energy intake from snacks was higher and that from breakfast was lower than the ideal ratio, respectively. Daily mean consumption of liquid was 1,526.4 mL/d for males and 1,151.5 mL/d for females, and these intakes were more than the KDRI (1,300 mL/d for males and 1,100 mL/d for females). However, their sources of liquid consumption were not desirable because alcohol, soda, juice of fruit or vegetable and beverage mixed with fruit juice and/or vegetable juice were major sources of liquid as well as drinking water. These findings show that university students have poor dietary behaviors including frequent skipping of breakfast, irregularity of meals, inadequate intakes of nutrients and undesirable pattersirablliquid consumption including high portion of alcohol and soda as alliquid source, and these trends were stronger for males than for females. Therefore, we should endeavor to correct their meal problems id ated to dietary behaviors, nutrient intakes and liquid consumptions through nutrition education.

Increased Serum S-TRAIL Level in Newly Diagnosed Stage-IV Lung Adenocarcinoma but not Squamous Cell Carcinoma is Correlated with Age and Smoking

  • Kargi, Aysegul;Bisgin, Atil;Yalcin, Arzu Didem;Kargi, Ahmet Bulent;Sahin, Emel;Gumuslu, Saadet
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권8호
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    • pp.4819-4822
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    • 2013
  • Background: Lung cancer is the leading cause of cancer mortality in the world. Many factors can protect against or facilitate its development. A TNF family member TRAIL, has a complex physiological role beyond that of merely activating the apoptotic pathway in cancer cells. Vitamin D is converted to its active form locally in the lung, and is also thought to play an important role in lung health. Our goal was to investigate the possible clinical significance of serum sTRAIL and 1,25-dihydroxyvitamin D(3) levels in patients with non-small cell lung cancer (NSCLC). Materials and Methods: Totals of 18 consecutive adenocarcinoma and 22 squamous cell carcinoma patients with stage-IV non-small cell lung cancer referred to our institute were included in this study. There were 12 men and 6 women, with ages ranging from 38 to 97 (mean 60.5) years with adenocarcinoma, and 20 men and 2 women, with ages ranging from 46 to 80 (mean 65) years with squamous cell carcinoma. Serum levels of sTRAIL and 1,25-dihydroxyvitamin D(3) were measured in all samples at the time of diagnosis. Results: sTRAIL levels in NSCLC patients were higher than in the control group. Although there was no correlation between patient survival and sTRAIL levels, the highest sTRAIL levels were correlated with age and cigarette smoking in the adenocarcinoma patients. sTRAIL level in healthy individuals were correlated with serum 1,25-dihydroxyvitamin D(3). Conclusions: Serum sTRAIL concentrations were increased in NSCLC patients, and correlated with age and smoking history, but not with overall survival.

식물공장내 양액의 EC가 적겨자와 청경채의 생육 및 품질에 미치는 영향 (Effects of Different EC in Nutrient Solution on Growth and Quality of Red Mustard and Pak-Choi in Plant Factory)

  • 이상규;최장선;이준구;장윤아;남춘우;여경환;이희주;엄영철
    • 생물환경조절학회지
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    • 제21권4호
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    • pp.322-326
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    • 2012
  • 최근 식물공장에 대한 연구가 활성화 되면서 식물공장내 인공광을 이용한 쌈채소 재배가 점점 늘어나고 있다. 특히 쌈채소를 좋아하는 우리나라에서는 그 소비량이 매년 증가하고 있으며, 토양재배보다는 수경재배로 생산한 엽채류를 좋아하기 때문에 식물공장 시설을 이용하여 수경재배로 쌈채소를 생산하면 그 효과가 크리라 생각된다. 따라서 본 실험은 식물공장내에서 쌈채소로 많이 이용되고 있는 적겨자와 청경채를 대상으로 양액농도별 수량과 비타민 함량의 차이를 분석하기 위하여 실시하였다. 그 결과, 적겨자와 청경채 모두 초장은 EC에 따른 유의성은 없었고, 엽수는 적겨자의 경우 EC 가 높아질수록 감소하는 경향을 보였으나 청경채의 경우는 유의성이 없었다. 청경채의 경우 EC가 높아질수록 엽면적이 현저하게 증가하였고, 생체중은 두 작물 모두 EC가 증가할수록 증가하는 경향을 보였다. 광합성 능력은 적겨자의 경우 뚜렷한 경향을 보이지 않았으나 청경채는 EC가 높을 때 높은 경향을 보였다. Ascorbic acid 함량은 적겨자의 경우에 EC가 낮을수록 높게 나왔고, 청경채의 경우에는 EC $2.0dS{\cdot}m^{-1}$ 처리구에서 가장 높았고, 1.5, $2.5dS{\cdot}m^{-1}$ 처리구 순이었다. 이상의 결과를 종합해 보면, 인공광 이용형 식물공장에서 적겨자와 청경채를 생산하고자 할 때에는 양액농도를 $2.0{\sim}2.5dS{\cdot}m^{-1}$로 관리하는 것이 생체중과 Ascorbic acid 함량을 고려해볼 때 가장 적절한 양액 농도 관리 방법이라고 생각된다.