• Title/Summary/Keyword: Vitamin $B_6$

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Nutritional Evaluation for Head, Feet and Tails Tissue of Pig (돼지머리, 족발, 꼬리의 영양학적(營養學的) 연구(硏究))

  • Ryu, Beoung-Ho;Kim, Hee-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.2
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    • pp.149-155
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    • 1984
  • Raw and cooked pig's head, feet and tails were evaluated for yield, proximate composition, caloric value, and the contents of amino acids, minerals and vitamins. The yields of the raw pig's head, feet and tails were 67.4%, 46.1% and 67.6%, respectively. Corresponding values for the cooked were 54.1%, 34.3% and 47.6% respectively. No significant differences were observed for proximate composition between these samples and any other source3 of the meats. The high energy values, 253.8-310.5 Cal/100 g, of the samples were largely due to the fat content. Among the essential amino acids, these products showed a higher lysine, leucine and threonine content, and lower methionine and tryptophan content. The total amounts of non-essential amino acids were more than half of those of all amino acids. Neither the raw nor the cooked pig's head, feet and tails contained vitamin A. On these data, pig's head, feet and tails tissue have a relatively low in vitamin B content as compared to conventional pork cuts.

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A Study of Premenstrual Syndrome(PMS) and the Nutritional Intake of College Women Residing in Busan Metropolitan City (부산지역 여대생의 월경전 증후군 실태 및 영양섭취상태에 관한 연구)

  • 황혜진;김영만
    • Korean Journal of Community Nutrition
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    • v.7 no.6
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    • pp.731-740
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    • 2002
  • This study was designed to identify premenstrual syndrome (PMS) and to investigate the correlation between premenstrual syndrome and nutritional intakes. The subjects of this study were 138 college women residing in Busan Metropolitan City. The subjects were asked to complete Menstrual Discomfort Questionnaire (MDQ) regarding PMS, food intake frequencies and nutritional intake. We studied the correlation between PMS symptoms and nutritional intake. The average height and weight of anemic subjects were 161.42 $\pm$ 3.50 cm and 51.87 $\pm$ 5.42 kg. The average BMI (body mass index, kg/$m^2$) was 19.92 $\pm$ 2.14 and PIBW (percent ideal body weight) were 93.02 $\pm$ 9.75%. Except for phosphorous, vitamin A and vitamin C intakes, the intake levels of all other nutrients were below the Korean RDA. The average calorie intake of the subjects was 1645.65 $\pm$ 352.63 kcal (82.2% of the Korean RDA) and iron intake of the subject was 11.06 $\pm$4.03 mg (69.1% of the Korean RDA) . The calcium and vitamin $B_6$ intakes were 512.26 $\pm$ 183.12 mg (73.1% of the Korean RDA) and 1.12 $\pm$ 0.14 mg (80.0% of the Korean RDA), respectively. With regard to their menstrual state, 45.9% subjects responded that their menstrual cycles were irregular. The frequency of PMS of the subjects was 30.2% (over 3 points) on 5 point scale (1 : no. 5 severe). The common symptoms of PMS of the subjects were pain (2.32 $\pm$ 1.01), negative effects (2.27 $\pm$ 0.87), behavioral changes (2.26 $\pm$ 0.85), water retention (2.07 $\pm$ 0.78) , arousal (1.79 $\pm$ 0.84) , autonomic reactions (1.77 $\pm$ 0.87) , lack of control (1.69 $\pm$ 0.75) and decreased concentration (1.68 $\pm$ 0.75) . There was significant correlation between all the PMS symptoms and calcium (p < 0.01) , vitamin E (p < 0.05) , carbohydrate (p < 0.05) . This suggests that PMS represents the clinical manifestation of nutrient deficiency states especially calcium. Therefore we concluded that calcium supplementation is likely to be of benefit in relieving PMS symptoms.

Functional Expression of Soluble Streptavidin in Escherichia coli (수용성 streptavidin의 Escherichia coli 에서 기능적 발현)

  • Han, Seung Hee;Kim, Hyeong Min;Lim, Myeong Woon;Kim, Jin-Kyoo
    • Journal of Life Science
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    • v.25 no.6
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    • pp.631-637
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    • 2015
  • Streptavidin, a protein produced by Streptomyces avidinii, strongly binds up to four molecules of vitamin H, d-biotin exhibiting the dissociation constant of about 10−15 M. This strong binding affinity has been applied for detection and characterization of numerous biological molecules suggesting expression and purification of functional streptavidin should be very useful for the application of this streptavidin-biotin interaction. To express a soluble streptavidin in Escherichia coli, We synthesized streptavidin genes and cloned into pET-22b plasmid, which uses T7 RNA polymerase/T7 promoter expression systems containing pelB leader for secretion into periplasmic space and six polyhistidine tags at C-terminus for purification of expressed proteins. Although streptavidin is toxic to Escherichia coli due to strong biotin binding property, streptavidin was expressed very sufficiently in a range of 10-20 mg/ml. In SDS-PAGE, the size of purified protein was shown as 17 kDa in denatured condition (boiling) and 68 kDa in native condition (without boiling) suggesting tetramerization of monomeric subunit by non-covalent association. Further analysis by size-exclusion chromatography supported streptavidin’s tetrameric structure as well. In addition, soluble streptavidin detected biotinylated proteins in westernblot indicating its functional activity to biotin. Taken these results together, it concluded that our simple expression system was able to show high yield, homotetrameric formation and biotin binding activity analogous to natural streptavidin.

Studies on Body Fat Ratio and Nutrient Intakes of College Students

  • Lee, Ji-Hyang;Song, Kyung-Hee
    • Nutritional Sciences
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    • v.8 no.1
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    • pp.56-60
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    • 2005
  • The aim of this study researchs status of the nutrient intake between male and female students and the nutrient intake by BMI. This study was done to investigate the body composition and nutrient intakes of 340 college students (170 males, 170 females) in Gyeonggi area. Weight, height, BMI, and blood pressure of these college students were measured Nutrient intake was assessed using the 24-hour recall method, and then calculated using the nutritional evaluation program [CAN Pro 2.0 professional (Korean Nutrition Society, 2002)]. Average height and weight were 176$\pm$5.1 cm, 70.60$\pm$l.l kg in male students and 162.2/$\pm$4.5 cm, 54.4$\pm$7.7 kg in female students respectively. Subjects were divided into three groups by BMI: underweight (less than 18.5), normal weight (18.5 to less than 23), and overweight (more than 23). The ratios of male and female subjects among these groups were 5.3% and 17.6% in underweight, 50% and 65.3% in normal weight, and 44.7% and 17.2% in overweight. Systolic and diastolic blood pressures were 124.0$\pm$1.5 mmHg and 76.1$\pm$14.8 mmHg in males and 1l0.l$\pm$12.9 mmHg and 70.l$\pm$10.8 mmHg in females, all in the normal range. Using nutrient values of the 7th Recommended Dietary Allowanes, the nutrient intake was calculated to Korean RDA (%). Nutrients below 75% Korean RDA were Calcium, Vitamin $B_2$, and Folate and nutrients above 100% RDA were Protein, Fe, Phosphorous, and Vitamin $B_6$. BMI showed positive correlations with body fat (%)(p<0.01) and WHR (p<0.01). Nutritional education is desirable for the improvement of dietary behaviors, body composition and nutrient intake of college students.

Development of Nutrition Education Material for Nutrient Intake and Prevention of Disease and the Effects of Nutrition Education for the Elderly - Focused on Items related to Health and Nutrients Intake - (노인을 위한 영양소 섭취와 질병예방에 대한 영양교육 자료 개발 및 영양교육의 효과 - 건강관련 사항과 영양소섭취량 중심으로 -)

  • Kim, Myoung-Sook;Kim, Ju Yeon
    • The Korean Journal of Community Living Science
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    • v.23 no.4
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    • pp.467-478
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    • 2012
  • This study was carried out to develop nutrition education materials for nutrient intake and the prevention of disease for the elderly and to test their effects on the health-related matters and nutrition intakes after nutrition education. The mean age of the subjects was 71.6 years old. Diet adjustment for health increased a little bit from 2.2% to 11.1% after education but with no significance. The highest reported chronic illness was hypertension. The drinking of the subjects decreased a little bit after their education but wasn't significant. There were no significant changes to the frequency of food consumption across all the items after education, but there was a small increase to the daily intake of milk and dairy products and fruits and to a balanced diet three times per day. There was a significant increase in the intake of such nutrients as energy(p<0.05), protein(p<0.01), lipid(p<0.05), phosphorus(p<0.05), sodium (p<0.05), vitamin $B_2$(p<0.05), vitamin $B_6$(p<0.05), and niacin(p<0.001) after education. Those findings show that nutrition education provided to the elderly had no clear effects on the items that required memorization such as nutrition knowledge but did have some effects on dietary life. If the nutrition education requiring memorization is provided repeatedly with data supplemented, more effective nutrition management will be possible.

Development of Artificial Diets for Green Lacewing, Chrysopa pallens (Rambur), by Addition of Natural Products (천연첨가물을 이용한 칠성풀잠자리붙이 인공사료 개발)

  • 최만영;이건휘;백채훈;이종진
    • Korean journal of applied entomology
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    • v.39 no.2
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    • pp.99-103
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    • 2000
  • The green lacewing, Chrysopa pallens (Rambur), was reared on artificial diets supplementedwith natural products such as lyophilized beef liver, silk worm pupae powder and drone honey beepowder. The performance of the green lacewing was improved siginificantly on only the diet to whichadded 1 % of silk worm pupae powder (wt/v). Larval developement was completed in 15.6 f 0.99 days,cocoon weight was 16.4f2.94 mg, and suvival to adult was 89.3% at 27$^{\circ}$C. And another diet made bymixing 4 part of lyophilized beef liver powder, 4 part of lyophilized beef powder, 3 part of lyophilizedchicken egg, 4 part of sucrose, and 50 mg each of vitamin C and Vanderzant's vitamin B mixture waspromising. Larval development of the green lacewing on this diet was completed in 12.7 f 1.45 days at27"C, pupal weight was 18.1 f 2.24mg, suvival to adult was loo%, and 633f70.4 eggs were laid in 20days after beginning of oviposition.ginning of oviposition.

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A Study on Premenstrual Syndrome (PMS), Nutrient Intake, Psychological Index according to the Obesity Degree of College Women (여대생의 비만도에 따른 월경전증후군 증상, 영양섭취 실태 및 심리지수 연구)

  • Kang, Se-Na;Park, Jung-Hwa;Hwang, Hye-Jin
    • Korean Journal of Community Nutrition
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    • v.16 no.1
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    • pp.14-22
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    • 2011
  • This study was designed to compare the incidence and severity of premenstrual syndrome (PMS) between normal (N = 85) and overweight or obese (N = 28) college female students and investigated correlation between PMS, nutrient intake, hematological index and psychological index (depression, anxiety, stress). Each subject was asked a Menstrual Discomfort Questionnaire (MDQ) for PMS by 5 Likert scale. The PMS scores of women in the normal weight subjects ranked in order of severity were water retention (2.71), followed by behavioral change (2.58), negative affect (2.46), pain (2.31), autonomic reaction (2.27), decreased concentration (2.16). The symptoms of 'pain' and 'behavioral change' of overweight or obese subject were significantly higher than those of normal subject (p < 0.05). And total cholesterol concentration of overweight or obese subjects was significantly higher than in normal subject (p < 0.05). There was a significant positive correlation (p < 0.05) between the symptoms of 'negative effect' and BMI. And the triglyceride concentration was positively related with 'water retention (p < 0.01)'. The symptoms of 'decreased concentration' were negatively correlated with calcium (p < 0.01) and vitamin B6 intake (p < 0.05). The depression score were positively related with symptoms of 'behavioral change (p < 0.05)', 'negative affect' (p < 0.01), and the anxiety score was positively correlated with 'behavioral change (p < 0.05)' and 'decreased concentration (p < 0.05)'. The stress score was positively correlated with 'decreased concentration (p < 0.01)', 'behavioral change (p < 0.05)' and 'negative affect (p < 0.05)'. This suggests that PMS represents the clinical manifestation of a calcium, vitamin $B_6$ deficiency and psychological disorder. Therefore we concluded that nutrient supplementation, depression and stress management may help to relieve PMS symptoms.

Comparison of Dangguibohyel-tang and Erythropoietin on Cyclophosphamide-induced Anemia in Rats (당귀보혈탕(當歸補血湯)과 eryhropoietin이 cyclophosphamide로 유도된 흰쥐의 빈혈에 미치는 영향 비교 연구)

  • Kang Soon-Ah;Chang Mun-Seog;Oh Myung-Sook;Kim Do-Rim;Kim Ji-Sook;Park Seong-Kyu
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.1
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    • pp.31-36
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    • 2006
  • The aim of this study was to elucidate the mechanism of anemia associated with Dangguibohyel-tang (DBT) in rats. Using cyclophosphamide-induced (30mg/kg BW) anemic rats, changes in weight gain, the levels of red blood cell (RBC), hematocrit (Hct), platelet and hemoglobin (Hgb), serum vitamin B12, ALT(GPT) levels and erythropoietin (EPO) gene expression were monitored, and compared with DBT (1,000mg/kg BW, 14d)-treated and EPO (1,000IU/kg BW, 14d, s.c.)-treated rats. Food efficiency ratio (FER) were 31.6%, in normal group, 28.1% in cyclophosphamide-induced control group, 31.7% in DBT-treated group and 25.1%, in EPO-treated group after 14 days. The levels of red blood cell (RBC), hematocrit (Hct), platelet and hemoglobin (Hgb) of DBT-treated group were significantly higher than those of control. And DBT extract administered group showed dominant effects on the recovery of Hgb level. Serum vitamin B12 and ALT(GPT) levels were significantly increased at DBT-treated groups. EPO gene expression was decreased 91,9% in control group, 79.6% in DBT-treated group and 53.9% in EPO-treated group, respectively. These results suggest that administration of DBT could prevent human patient from chemotherapy derived anemia by improving hematological value and EPO status.

Assessment of dietary quality and nutrient intake of obese children in Changwon area

  • Ji-Sook Park;Ha-Neul Choi;Jae-Young Kim;Sang-Hyuk Ma;Jung-Eun Yim
    • Journal of Nutrition and Health
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    • v.55 no.6
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    • pp.630-641
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    • 2022
  • Purpose: In this study, we assessed the intake of nutrients and food groups and analyzed the nutrition quotient of obese children in South Korea. The hypothesis was that dietary quality and nutrient intake are related to the body mass index (BMI) of obese children. Methods: The participants included 48 children (20 boys and 28 girls). Based on the guidelines for the age-for-body mass index provided by the Korean National Growth Charts for children and adolescents, the participants were divided into 3 groups: overweight, BMI ≥ 85th percentile; obese, BMI ≥ 95th percentile; severely obese, BMI ≥ 99th percentile. Results: The energy distribution showed that the carbohydrate ratio was significantly higher in the overweight group (p < 0.01), whereas the fat ratio was significantly higher in the obese and severely obese groups (p < 0.05). Thus, in the overweight and obese groups, the percent body fat was negatively associated with the carbohydrate ratio (p < 0.05) but was positively associated with the fat ratio (p < 0.05). The Dietary Reference Intakes (DRI) data revealed that the intake levels of protein, vitamin B1, vitamin B12, and sodium were higher in all groups. Intakes of fiber, calcium, potassium, and magnesium were insufficient in all groups, and the DRI percent for protein was significantly higher in the obese and severely obese groups than in the overweight group. No significant differences were obtained in food group patterns or Nutrition Quotient (NQ) scores among the 3 groups. According to the evaluation results by food record and NQ, a significant difference was obtained in the dietary quality of obese children. Conclusion: We conclude that a higher intake of fat enhances weight gain. Based on the study results, we propose that the guidelines should consider the energy distribution of carbohydrate and fat intake to prevent and control obesity among Korean children.

Anthropometric Measurement, Dietary Behavior and Nutrient Intake of the Nation-Wide College Students Attending a Nutrition Education via Internet (인터넷 영양교육 참여 전국 대학생의 지역별 신체계측, 식행동 및 영양소 섭취상태에 관한 연구)

  • Cheong, Sun-Hee;Chang, Kyung-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.5
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    • pp.565-571
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    • 2006
  • The purpose of this study was to investigate the regional differences in anthropometric measurement, dietary behavior and nutrient intake among the nation-wide college students participating in a nutritional education program via internet. The subjects were 1614 college students (male: 752, female: 862) and divided into 4 regional groups. A cross-sectional survey was conducted using a self administered questionnaire, and the data were collected via internet or by mail. The nutrient intake data collected from food record were analyzed by the Computer Aided Nutritional Analysis Program. Data were analyzed by SPSS 10.0 program. Average age of male and female college students were 23.6 years and 21.0 years, respectively. Most of the male and female college students had poor eating habits, such as unbalanced meals and skipping meals. In particular, more than 60% of the male college students in Chungcheong and Gyeongsang areas had irregularity of meal time. Female students in Seoul and Incheon areas showed significantly higher consumption frequency of yellow and green leafy vegetables compared to Chungcheong and Gyeongsang areas. Female students in Seoul, Incheon and Chungcheong areas showed significantly higher consumption frequency of milk and milk products compared to Gyeongsang area. Calorie, vitamin A, calcium and iron intakes of the male students and calorie, vitamin A, vitamin $B_2$, niacin and calcium intakes of female students were lower than the Korean RDA. Female students in Incheon area showed significantly higher vitamin C and iron intakes compared to the other areas. These results provide a nation-wide information on dietary behavior and nutrient intake among Korean college students.