• Environmental Mutagens and Carcinogens
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• v.21 no.2
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• pp.89-94
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• 2001

Recently, single cell gel electrophoresis, also known as comet assay, is widely used for the detection and measurement of DNA strand breaks in vitro and in vivo in many toxicological fields such as radiation exposure, human monitoring and toxicity evaluation. As well defined, comet assay is a sensitive, rapid and visual method for the detection of DNA strand breaks in individual cells. Briefly, a small number of damaged cells suspended in a thin agarose gel on a microscope slide were lysed, unwinded, electrophoresed, and stained with a fluorescent DNA binding dye. The electric current pulled the charged DNA from the nucleus such that relaxed and broken DNA fragments migrated further. The resulting images which were subsequently named for their appearance as comets, were measured to determine the extent of DNA damages. However, some variations could be occurred in procedures, laboratories's conditions and kind of cells used. Hence, to overcome and to harmonize these matters in comet assay, International Workshop on Genotoxicity Test Procedure (IWGTP) was held with several topics including comet assay at Washington D.C. on March, 1999. In spite of some consensus in procedures and conditions in IWGTP, there are some problems still remained to be solved. In this respect, we attempted to set the practical optimal conditions in the experimental procedures such as lysis, unwinding, electrophoresis and neutralization conditions and so on. First of all, we determined optimal lysis and unwinding time by using 150 $\mu$M methyl methanesulfonate (MMS) which is usually used concentration. And then, we determined optimal positive control concentrations of benzo(a)pyrene (BaP) and MMS in the presence and absence of S9 metabolic activation system, respectively.

• Journal of the Korean Institute of Gas
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• v.4 no.2 s.10
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• pp.37-45
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• 2000

The underground buried pipelines of Natural gas are relatively safer than any other pipelines of chemical plants, because Natural gas is non-corrosive fluid. But Natural gas is supplied normally the downtown area. So, it may be a disaster because of corrosion which is caused interference facilities, environment and third party accident which is caused facilities construction. Especially, it is very difficult to find out and inspect damages of pipeline because of buried pipelines. Therefore this paper approached to select and manage risk region pipelines according to introduction of underground buried pipeline's risk concept. Risk was indicated three parts - corrosion factor, design and construction factor, maintence and management factor - in this paper, Therefore qualitive risk of pipelines showed score as quantitative number. Also it was thought to be helpful in confidence and safety management that the concept of key index and failure supplementation measures to cost introduces this program. We developed this risk assessment program using visual basic tool and interfaced GIS.

• Journal of the Korean Geotechnical Society
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• v.28 no.2
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• pp.15-21
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• 2012

It is difficult to determine the strength and the permeability of a soil levee quantitatively because the damages of a levee have been evaluated only by visual inspection in Korea. Therefore, it is necessary to develop a simplified measurement device for measuring the strength and permeability of a levee. In this paper, a new in-situ permeability measuring device which can measure strength and permeability based on GWP is developed and its applicability to the field permeability test of a soil levee is introduced. From the laboratory and in-situ experiments, the new measuring device has the potential of measuring the soil permeability simply and accurately.

• Structural Engineering and Mechanics
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• v.54 no.2
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• pp.257-289
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• 2015

Shear connectors are generally used to link the slab and girders together in slab-on-girder bridge structures. Damage of shear connectors in such structures will result in shear slippage between the slab and girders, which significantly reduces the load-carrying capacity of the bridge. Because shear connectors are buried inside the structure, routine visual inspection is not able to detect conditions of shear connectors. A few methods have been proposed in the literature to detect the condition of shear connectors based on vibration measurements. This paper proposes a different dynamic condition assessment approach to identify the damage of shear connectors in slab-on-girder bridge structures based on power spectral density transmissibility (PSDT). PSDT formulates the relationship between the auto-spectral densities of two responses in the frequency domain. It can be used to identify shear connector conditions with or without reference data of the undamaged structure (or the baseline). Measured impact force and acceleration responses from hammer tests are analyzed to obtain the frequency response functions at sensor locations by experimental modal analysis. PSDT from the slab response to the girder response is derived with the obtained frequency response functions. PSDT vectors in the undamaged and damaged states can be compared to identify the damage of shear connectors. When the baseline is not available, as in most practical cases, PSDT vectors from the measured response at a reference sensor to those of the slab and girder in the damaged state can be used to detect the damage of shear connectors. Numerical and experimental studies on a concrete slab supported by two steel girders are conducted to investigate the accuracy and efficiency of the proposed approach. Identification results demonstrate that damages of shear connectors are identified accurately and efficiently with and without the baseline. The proposed method is also used to evaluate the conditions of shear connectors in a real composite bridge with in-field testing data.

• Smart Structures and Systems
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• v.11 no.6
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• pp.589-604
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• 2013

Identification of damage has become an evolving area of research over the last few decades with increasing the need of online health monitoring of the large structures. The visual damage detection can be impractical, expensive and ineffective in case of large structures, e.g., offshore platforms, offshore pipelines, multi-storied buildings and bridges. Damage in a system causes a change in the dynamic properties of the system. The structural damage is typically a local phenomenon, which tends to be captured by higher frequency signals. Most of vibration-based damage detection methods require modal properties that are obtained from measured signals through the system identification techniques. However, the modal properties such as natural frequencies and mode shapes are not such good sensitive indication of structural damage. Identification of damaged jacket type offshore platform members, based on wavelet packet transform is presented in this paper. The jacket platform is excited by simple wave load. Response of actual jacket needs to be measured. Dynamic signals are measured by finite element analysis result. It is assumed that this is actual response of the platform measured in the field. The dynamic signals first decomposed into wavelet packet components. Then eliminating some of the component signals (eliminate approximation component of wavelet packet decomposition), component energies of remained signal (detail components) are calculated and used for damage assessment. This method is called Detail Signal Energy Rate Index (DSERI). The results show that reduced wavelet packet component energies are good candidate indices which are sensitive to structural damage. These component energies can be used for damage assessment including identifying damage occurrence and are applicable for finding damages' location.

• Journal of Korean Tunnelling and Underground Space Association
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• v.9 no.1
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• pp.19-28
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• 2007

Tunnel management is a time-consuming and expensive task. In particular, visual analysis of tunnel inspection often requires extended time and cost and shows problems on data gathering, storage and analysis. This study proposes a new approach to extract information for tunnel management by using a laser scanning technology. A prototype tunnel laser scanner developed was used to obtain point clouds of a railway tunnel surface. Initial processing of laser scanning data was to separate those laser pulses returned from the installations attached to tunnel liner using radiometric and geometric characteristics of laser returns. Once the laser returns from the installations were separated and removed, physically damaged parts on tunnel lining are detected. Based on the plane formed by laser scanner data, damaged parts are detected by analysis of proximity. The algorithms presented in this study successfully detect the physically damaged parts which can be verified by the digital photography of the corresponding location on the tunnel surface.

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.2
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• pp.61-69
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• 2009

In mid-July, 2006 the torrential rainfall across Gangwon-do region caused 48 casualties and 1,248 houses submerged, resulting in damages with the restoration costs of 3 trillion and 512.5 billion won. This was because the topographic characteristics of Gangwon-do region for which mountainous areas mostly account increased the effects of landslide. In this study, the landslide region was shot using the PKNU No.4 system immediately after the occurrence of landslide in order to analyze it as objectively, exactly, and rapidly as possible. 1,054 areas with landslide occurrence were extracted by digitizing the shot images through visual reading after orthometric correction using ERDAS 9.1. Using the Arc GIS 9.2, a GIS program, hydrologic, topographic, clinical, geologic, pedologic aspects and characteristics of the landslide region were established in database through overlay analysis of digital map, vegetation map, geologic map, and soil map, and the status and characteristics of the occurrence of the landslide were analyzed.

• Korean Journal of Human Ecology
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• v.16 no.5
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• pp.1051-1059
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• 2007

This study intends to find out possible variations in physical properties and color of hair depending on dye type(acidic and alkalic) and permanent treatment/hair dyeing procedure. To meet the goals, healthy hair samples were taken from 5 women's hair speciment in 20's without experiencing in any harmful substance via long-term medications. and beauty care using chemicals. To get possible findings depending upon hair dye type and treatment procedure, both acidic and alkalic hair dye were applied on some specimens after permanent treatment, while on other specimens before permanent treatment. In order to determine scale damage, this study used SEM(Hitachi S-2500C) both hair thickness and tensile strength were measured with optical microscope(Nikon, MM-60/L3T) and Instron (4482-standard). Wave configuration was measured transverse and longitudinal round diameter. The chromaticity of each specimen was measured using a spectrocolorimeter(Color Techno System, JP/JX-777) with visual $C/2^{\circ}$ (at $2^{\circ}$ with C light source) monitor fixed to determine Lab and CMYK values. As a result, it was found that hair specimens were more significantly damaged with alkalic hair dye treatment than with acidic hair dye treatment, while hair specimens were more effectively dyed with the former hair dye type than with the latter one. For possible results depending on treatment procedure, it was found that hair specimens were less damaged but more effectively dyed with permanent treatment followed by hair dyeing than vice versa. Therefore, it was concluded that permanent treatment followed by acidic hair dyeing would be more effective in reducing hair damages.

• Smart Structures and Systems
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• v.21 no.3
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• pp.305-320
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• 2018

The second half of the 20th century was marked with a significant raise in amount of railway bridges in Austria made of reinforced concrete. Today, many of these bridges are slowly approaching the end of their envisaged service life. Current methodology of assessment and evaluation of structural condition is based on visual inspections, which, due to its subjectivity, can lead to delayed interventions, irreparable damages and additional costs. Thus, to support engineers in the process of structural evaluation and prediction of the remaining service life, the Austrian Federal Railways (${\ddot{O}}$ BB) commissioned the formation of a concept for an anticipatory life cycle management of engineering structures. The part concerning concrete bridges consisted of forming a bridge management system (BMS) in a form of a web-based analysis tool, known as the LeCIE_tool. Contrary to most BMSs, where prediction of a condition is based on Markovian models, in the LeCIE_tool, the time-dependent deterioration mechanisms of chloride- and carbonation-induced corrosion are used as the most common deterioration processes in transportation infrastructure. Hence, the main aim of this article is to describe the background of the introduced tool, with a discussion on exposure classes and crucial parameters of chloride ingress and carbonation models. Moreover, the article presents a verification of the generated analysis tool through service life prediction on a dozen of bridges of the Austrian railway network, as well as a case study with a more detailed description and implementation of the concept applied.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.37 no.10
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• pp.1017-1026
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• 2009

The solar cell system operates by facing the sun-light. Minor cracks, static discharge, and thermal shock that can happen during production/testing phase can lead to degradation in performance during operation, since solar cells are exposed to extreme thermal/mechanical environment in space. In order to detect small cracks and internal damages in the solar cells due to thermal shocks, which are the core units of a solar cell system, expensive equipment, complicated test process, and much time are required. Therefore, a qualitative method for easily and quickly testing the 'health' of solar cell functionality is required. This dissertation describes a theoretical and technical grounds for quickly and easily evaluating the health of solar cells using electroluminescence effect of Gallium-Arsenide solar cells that are most widely used by spacecrafts in recent years. Also described in the dissertation is the technical issues and constraining factors for applying the proposed method to actual space-rated solar cell systems.