• 사상체질의학회지
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• 제30권2호
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• pp.8-27
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• 2018

Objective This study investigated the effects of Jeoreongchajeonja-tang in a high-fat diet-induced obesity mouse model. Methods The study examined 9-week-old male mice (C57bl/6J) divided into four groups: the normal(C57bl/6J-Nr), control (high-fat diet only; HFD-CTL), positive-control (high-fat diet with Garcinia cambogia), and experimental (high-fat diet with Jeoreongchajeonja-tang; HFD-JCT) groups. After 7 weeks, the body weight, food efficiency ratio, organ weight, and visceral fat weight of the mice were measured. Blood serum tests, mRNA, liver histopathology, and epididymis adipocytes were also examined. Results Compared with the Control(HFD-CTL) group, the Experimental(HFD-JCT) group given Jeoreongchajeonja-tang showed significant reductions in absolute body weight and food efficiency ratio. The serum alanine aminotransferase, total-cholesterol, triglyceride, low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein (HDL)-cholesterol, insulin-like growth factor-1, and leptin levels were significantly lower in the experimental group than in the control group. The serum adiponectin levels were significantly higher in the experimental group than in the control group. Compared with the control group, the experimental group showed significant reductions in absolute abdominal subcutaneous fat, epididymal adipose tissue, kidney adipose tissue, intestine adipose tissue, and liver, kidney and spleen adipose tissue weights. The C/EBP-${\beta}$, leptin, and SREBP1c/ADD1 mRNA expression were significantly lower in the experimental group than in the control group, while the UCP-2 and adiponectin mRNA expression were significantly higher. Compared with the control group, the experimental group showed a significant reduction in the absolute adipocyte area in the liver and epididymal adipose tissue. Conclusion Jeoreongchajeonja-tang has an anti-obesity effect. Additional clinical studies are expected.

• 운동영양학회지
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• 제13권1호
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• pp.9-14
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• 2009

The effects of a hypocaloric diet with or without strength training on body fat distribution and serum lipid concentrations in obese elderly women were investigated. Twenty-six healthy women (age 66±4.6 yr; body mass index 32.3±2.9 kg/m²) were randomly assigned to 3 groups: control (C; n=8), hypocaloric diet (DO; n=9) or hypocaloric diet with strength training (DST; n=9). Subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and inter and intra muscular adipose tissue (IMAT) were measured using magnetic resonance imaging. Serum lipid concentrations including total cholesterol (TC), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), and triglycerides (TG) were measured. No significant changes occurred in body weight and percent body fat in the C group over the 16-week period. The DO and DST groups lost similar amounts of weight and fat after 16 weeks. SAT and VAT decreased after weight loss in the DO and DST groups but not in the C group. IMAT was significantly reduced in the DO and DST groups, whereas in the C group IMAT increased. The loss in IMAT mass was similar in the DO and DST groups. TC and LDLC decreased in the DO and DST groups but not in the C group. There were no differences between the DO and DST groups in decrease in TC and LDLC. HDLC decreased in the DO group but not in the C and DST groups. TG tended to decrease in the DST group. In conclusion, body fat distribution including SAT, VAT, and IMAT and serum lipid concentratons were modulated by weight loss resulting from the hypocaloric diet with or without strength training in obese elderly women. Strength training did not enhance the improvement in body fat distribution and serum TC and LDLC concentrations by the hypocaloric diet.

• 대한융합한의학회지
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• 제5권2호
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• pp.11-16
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• 2023

Objectives: The purpose of this study was to investigate the anti-obesity effects of the aqueous extract of Schizandra chinensis (SC) in menopausal mice. Methods: To induce menopausal obesity, female mice were ovariectomized (OVX) and fed a high-fat diet (HFD; 60% fat, 28% carbohydrates, 14% protein) for 12 weeks. The mice were divided into 6 groups (n = 8): NOR (sham-operated and vehicle-treated), HFD+OVX (vehicle-treated), E2 (17-beta estradiol 50 ㎍/kg-treated), SC1 (1 mg/kg SC-treated), SC10 (10 mg/kg SC-treated), and SC100 (100 mg/kg SC-treated). Samples were orally administered for 6 weeks, after which all experimental mice were sacrificed. Body weight, feeding efficiency, white adipose tissue weight, adipocyte diameter, and fat vacuoles in liver were analyzed. Results: By treating with SC extract, the body weight and feeding efficiency of mice were significantly decreased. The weight of visceral fat tissues was decreased in the SC10 and SC100 groups. Histopathology showed that fat cell diameters of white adipose tissue were also decreased in the SC10 and SC100 groups. Additionally, SC extract regenerated the hepatocyte damage and decreased the size and number of follicular adipocytes Conclusion: In summary, these results suggest that SC has inhibitory effects against menopausal obesity. Schizandra chinensis may be a potential alternative for obesity among female menopausal diseases.

• Nutritional Sciences
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• 제6권2호
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• pp.73-77
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• 2003

PAI-1 (plasminogen activator inhibitor-1), leptin, and resistin are synthesized and secreted by Int cells of rodents and have recently been postulated to be an important link to obesity. This study was conducted to identify the nutritional regulation of PAI-1, leptin, and resistin gene expression in 0b/ob mice. The mice were divided into four groups according to nutritional status: control, 48 hour fasting, 48 hour-fasting/12 hour-refeeding, and 48 hour-fasting/24 hour-refeeding. The mRNA levels of each peptide were measured by semi-quantitative RT-PCR. In visceral fat tissue, the level of PAI-1 mRNA increased markedly when 48h-fasted animals were refed with a high carbohydrate-low fat diet. However, lasting/refeeding did not appreciably change PAI-1 mRNA levels in subcutaneous fat tissue. Similar results were obtained for resistin mRNA levels in both types of fat tissues. These findings suggest that visceral adipose tissue might be more sensitively involved in the nutritional regulation of PAI-1 and resistin gene expression compared to subcutaneous fat tissue. The level of leptin mRNA decreased markedly in the 48h-fasted animals, and increased markedly when 48h-fasted animals were refed with a high carbohydrate-low fat diet. The nutritional regulation of leptin mRNA showed similar patterns in both types of fat tissues. In conclusion, the nutritional regulation of gene expression encoding PAI-1, resistin, and leptin from adipocytes may vary according to the type of adipose tissue.

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.576-583
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• 2009

G protein-coupled receptor 43 (GPR43) is a newly-discovered short-chain free fatty acid receptor and its functions remain to be defined. The objective of this study was to investigate the function of GPR43 on lipolysis. We successfully cloned the GPR43 gene from the pig (EU122439), and measured the level of GPR43 mRNA in different tissues and primary pig adipocytes. The expression level of GPR43 mRNA was higher in adipose tissue and increased gradually with adipocyte differentiation. Then we examined GPR43 mRNA level in different types, growth-stages and various regions of adipose tissue of pigs. The results showed that the expression level of GPR43 mRNA was significantly higher in adipose tissue of obese pigs than in lean pigs, and the expression level also gradually increased as age increased. We further found that the abundance of GPR43 mRNA level increased more in subcutaneous fat than visceral fat. Thereafter, we studied the correlation between GPR43 and lipid metabolism-related genes in adipose tissue and primary pig adipocytes. GPR43 gene had significant negative correlation with hormone-sensitive lipase gene (HSL, r = -0.881, p<0.01) and triacylglycerol hydrolase gene (TGH, r = -0.848, p<0.01) in adipose tissue, and had positive correlation with peroxisome proliferator-activated receptor $\gamma$ gene ($PPAR_{\gamma}$, r = 0.809, p<0.01) and lipoprotein lipase gene (LPL, r = 0.847, p<0.01) in adipocytes. In addition, we fed different concentrations of docosahexaenoic acid (DHA) to mice, and analyzed expression level changes of GPR43, HSL and TGH in adipose. The results showed that DHA down-regulated GPR43 and up-regulated HSL and TGH mRNA levels; GPR43 also had significant negative correlation with HSL (low: r = -0.762, p<0.01; high: r = -0.838, p<0.01) and TGH (low: r = -0.736, p<0.01; high: r = -0.586, p<0.01). Our results suggested that GPR43 is a potential factor which regulates lipolysis in adipose tissue, and DHA as a receptor of GPR43 might promote lipolysis through down-regulating the expression of GPR43 mRNA.

• Asian-Australasian Journal of Animal Sciences
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• 제13권5호
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• pp.702-710
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• 2000

A large portion of total energy expenditure associated with ruminant livestock production goes towards maintenance. Approximately 55% of whole body energy use is consumed by visceral tissues (including internal organs) with the majority of this going to the liver and gastrointestinal tract. Muscle and adipose tissues consume about 27% of total body energy expenditure. Metabolic components within the viscera responsible for the majority of energy consumption include ion transport, protein turnover, substrate cycling, and urea synthesis (liver). Within muscle tissue of growing animals ion transport and protein turnover account for most of the energy expenditure. Protein synthesis consumes approximately 23% of whole body energy use and visceral tissues account for proportionally more of whole body protein synthesis than skeletal muscle. Research efforts focused on improving energetic efficiency of the tissues and metabolic mechanisms responsible for the majority of whole animal energy expenditure should provide information leading to more efficient production of an edible product.

• 한국발생생물학회지:발생과생식
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• 제19권4호
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• pp.235-242
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• 2015

Black soybean teata is helpful to preventing obesity through enhancing energy expenditure and suppressing accumulation in mesenteric adipose tissue. The ethanol testa-extract of Cheongja #3 black soybean (ETCBS) is also have similar effects on obesity. So far, it is not clear whether the ethanol testa extract of black soybean can have effect on the characters of subcutaneous adipose stem cells such as proliferation, activity, and adipogenicity. The doubling time was different between subcutaneous adipose-derived stem (ADS) and visceral ADS cells. By the in vitro culture and passage, the doubling time was increased both of them. The shape was not different between groups and their passages were not cause the change of shapes. In the case of visceral ADS cells, the doubling time was 62.3 h or 40.3 h in control or high fat diet administrated mice, respectively, but not modified in subcutaneous ADS cells. ETCBS administration caused of increased the doubling time from 62.3 h to 84.2 h. ETCBS had suppressive effects on the cellular activity of subcutaneous ADS cells. The intensity of Oil Red O staining was very faint in 100 and $200{\mu}g/mL$ ETCBS treated groups. The amounts of accumulated triglyceride were also significantly low in 100 and $200{\mu}g/mL$ treated groups. From these results we know that the doubling times and the effects of ETCBS are different by the anatomical origin of ADS cells. It also suggested that ETCBS may suppress the differentiation of subcutaneous ADS cells into the precursors and maturing of adipocytes.

• Nutrition Research and Practice
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• 제5권1호
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• pp.11-19
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• 2011

Eotaxin is an important inflammatory chemokine in eosinophil chemotaxis and activation and, thus, is implicated in asthma. Recently, obesity was associated with an increased prevalence of asthma, but the relationship between obesity and eotaxin expression has only been partially understood in obese mice and human studies. Therefore, we studied the expression patterns of eotaxin in 3T3-L1 preadipocytes/adipocytes to determine whether eotaxin levels are influenced by body weight gain and/or reduction in diet-induced obese mice. First, we investigated eotaxin expression during differentiation in 3T3-L1 adipocytes. Then, we treated 3T3-L1 preadipoeytes/adipoeytes with tumor necrosis factor-alpha (TNF-${\alpha}$), eotaxin, interleukin (IL)-4, IL-5, or leptin. To examine the effects of weight loss in high-fat diet induced obese mice, we fed C57BL/6 mice a high-fat diet or a normal diet for 26 weeks. Then, half of the high-fat diet group were fed a normal diet until 30 weeks to reduce weight. Epididymal adipose tissue, visceral adipose tissue, serum, and bronchoalveolar fluid of mice were examined for eotaxin expression. The results showed that eotaxin expression levels increased with adipocyte differentiation and that more eotaxin was expressed when the cells were stimulated with TNF-${\alpha}$, eotaxin, IL-4, IL-5, or leptin. An in vivo study showed that eotaxin levels were reduced in visceral adipose tissues when high-fat diet fed mice underwent weight loss. Taken together, these results indicate a close relationship between eotaxin expression and obesity as well as weight loss, thus, they indirectly show a relation to asthma.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제13권2호
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• pp.172-179
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• 2010

목 적: 성인에서 내장 지방은 대사 이상의 위험 요소와 밀접한 상관이 있다고 알려져 있으나 소아 청소년 연령에서의 연구는 미흡하다. 소아 청소년에서의 복부지방의 분포와 대사 이상의 위험 요소간에 상관성을 연구하고자 하였다. 방 법: 78명의 남아와 23명의 여아를 포함한 101명의 소아 청소년(평균 연령 10.8${\pm}$2.4세)을 대상으로 연구를 시행하였다. 신체 계측과 대사 증후군의 위험 요소에 대한 분석을 개별 환자에서 시행하였고, 복부 전산화 단층 촬영을 통해 내장 지방과 피하 지방의 양을 측정하였다. 연령을 보정한 편 상관 분석을 통해 내장 지방, 피하 지방의 양, 신체 계측 지표와 대사 이상의 위험요소 간의 상관관계를 분석하였다. 결 과: 남아와 여아 모두에서 피하 지방이 나이가 들어감에 따라서 내장 지방보다 급격히 증가하는 것을 확인할 수 있었다. 남아에서 내장 지방과 피하 지방은 신체 계측 지표와는 유의한 상관성을 보였고, 내장 지방은 낮은 수치의 HDL-C과 피하 지방은 높은 이완기 혈압과 유의한 연관성을 보였으나. 남아에서 내장 지방과 피하 지방, 신체 계측 지표는 모두 대사 이상의 위험요소의 수와 밀접한 관계가 있는 것으로 나타났다. 결 론: 남아에서 내장 지방은 낮은 수치의 HDL-C과, 피하 지방은 높은 이완기 혈압과 유의한 상관성을 나타내었으나 성인에서의 기존 연구에 비해 본 연구는 다른 대사 이상의 위험 요소들에 대한 상관성이 떨어지는 것으로 나타났다.

• Nutrition Research and Practice
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• 제7권1호
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• pp.43-48
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• 2013

Quantities as well as distributions of adipose tissue (AT) are significantly related to cardiovascular disease (CVD) risk factors and can be altered with caloric restriction. This study investigated which cross-sectional slice location of AT is most strongly correlated with changes in CVD risk factors after caloric restriction in obese Korean women. Thirty-three obese pre-menopausal Korean women ($32.4{\pm}8.5$ yrs, BMI $27.1{\pm}2.3\;kg/m^2$) participated in a 12 weeks caloric restriction program. Subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) were measured using computed tomography (CT) scans at the sites of L2-L3, L3-L4, and L4-L5. Fasting serum levels of glucose, insulin, triglyceride, total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), leptin and homeostasis model assessment-insulin resistance (HOMA-IR) were observed. Pearson's partial correlation coefficients were used to assess the relationship between AT measurement sites and changes in CVD risk factors after calorie restriction. When calories were reduced by 350 kcal/day for 12 weeks, body weight (-2.7%), body fat mass (-8.2%), and waist circumference (-5.8%) all decreased (P < 0.05). In addition, following caloric restriction, serum levels of glucose (-4.6%), TC (-6.2%), LDL-C (-5.3%), leptin (-17.6%) and HOMA-IR (-18.2%) decreased significantly (P < 0.05) as well. Changes in VAT at the level of L3-L4 were significantly greater than those at other abdominal sites, and these changes were correlated with changes in TC (P < 0.05), LDL-C (P < 0.001), SBP (P < 0.001) and HOMA-IR (P < 0.01). These results show that VAT at L3-L4 had a stronger correlation with CVD risk factors than with other AT measurement sites after caloric restriction.