• Journal of Plant Biology
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• v.7 no.1
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• pp.1-4
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• 1964

A comparative study of free amino acid content in healthy and virus diseased tobacco leaves was carried out by author throughout the gorwing season from June to November of 1963. The methods of qualitative analysis of free amino acids applied in this experiment is followed by Moore and Stein. 1,2 Free amino acids determined in this experiment are shown in Fig. Ⅰ, Ⅱ and Table Ⅰ. As the figure and the table are shown, four more amino acids such as a spartic acid, glutamic acid, tyrosine and phenylalanine are detected in the healthy leaves; these four additional amino acids in the healthy leaves are conspicuous. More quantities of asparagine and alanine are detected in the diseased leaves than the healthy leaves and more quantities of tryptophan is detected in the healthy leaves. It is presumed that such amino acids as tyrosine and phenyllanine are decreased by the incooperation of free amino acid to TMV protein in the process of the process of the leaf protein metabolism which is caused by TMV-RNA trapping action in the diseased leaf protoplasm. It is thought that the decrease of asparagine and the increase of asparic acid in the healthy leaves are the results of in incooperaton of NH2, produced by the protein dissimilation in the diseased leaves, to aspartic acid; it's reaction is caused by the respiration of the diseased leaves accelerated by TMV attack. It is presumed, consequently, that the check of the diseased tobacco leave growth is influenced by the reduction of such amino acids as tryptophane and glutamic acid, which reduction may be due to the abnormal protein metabolism and the action of certain enzyme caused by TMV attack on host protoplast.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.612-615
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• 2009

Lipoxygenase and Kunitz trypsin inhibitor protein are the main antinutritional factor in mature soybean seed. A new soybean cultivar, "Gaechuck#2" with yellow seed coat, lipoxygenase2,3-free and Kunitz trypsin inhibitor protein-free was developed. It was selected from the population derived from the cross between "Jinpumkong2ho" and C242. Plants of "Gaechuck#2" have determinate growth habit with purple flowers, tawny pubescence, yellow seed coat, yellow hilum, oval leaflet shape and brown pods at maturity. Seed protein and oil content on a dry weight basis were 40.7% and 18.7%, respectively. It has shown a resistant reaction to soybean necrosis, soybean mosaic virus, Cercospora leaf spot and blight, black root rot, pod and stem blight, and soybean pod borer. Gaechuck#2 matured in 4 October with plant height of 54cm and a 100-seed weight of 24.4g. Average Yield of Gaechuck#2 was 230 - 250 kg/10a in 2005 - 2007.

• Research in Plant Disease
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• v.9 no.4
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• pp.237-241
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• 2003

Damages caused by infection with Cucumber mosaic virus (CMV), Lily mottle virus (LMoV) and Lily symptomless virus (LSV) were assessed by comparing growth of plants produced from seeds of Lilium x fomolongi cultivar 'Noesan' both infected and free from infection with those viruses. Symptoms and infection rate were investigated in field-grown lily.Dominant viral infection symptom in the field was mottle on leaves, caused by natural infection with LMoV. Incidence of viral disease caused by mixed infection with two of LMoV, CMV and LSV in field-grown Lilium x fomolongi cultivars, reused for more than 6 years consecutively, was 80 percent. In comparison with healthy Lilium x fomolongi cultivar 'Noesan', plants doubly infected with CMV-Li1 and LMoV-Li diminished their plant height by 14 percent, fresh weight by 38 percent, and flower length by 15 percent. Lily plants singly infected with CMV-Li1 or LMoV-Li significantly reduced their freish weight by 21.8% and 28.4% compared to healthy plants, respectively.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.43-47
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• 2001

This study was conducted to investigate the integrated control strategy against pear black leaf spot disease. Using indicator plant PS-95 we selected six virus-free trees (cv. Niitaka) from commercial orchards in the major pear production area. Disease symptom by pear black leaf spot in the shoot of pear (Niitaka) has been successfully inhibited by heat treatments for 3 weeks at temperature of $40^{\circ}C$ (day) and $32^{\circ}C$ (night) or 4 weeks at $37^{\circ}C$ and $30^{\circ}C$, respectively. However, survival rate of the shoot tips on stocks was low. Seven cultivars Niitaka, Imamura- aki, Waseaka, Nijisseiki, Wasenijisseiki, Shinsetsu and Whangkeumbae produced visible symptoms in the first year after inoculation with the virus. Other 11 selections remained symptomless. Two resistant cultivars Suwhangbae and Gamcheonbae were selected, which showed high fruit quality. It is recommended that these cultivars could be top-grafted onto the infected Niitaka trees to overcome the damage caused by the disease in the orchard.

• Journal of Bio-Environment Control
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• v.20 no.3
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• pp.197-203
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• 2011

This experiment was carried out to investigate the growth characteristics of virus-free sweetpotato in hydroponic bag culture. The rooted one-node and upper-shoot cuttings of 'Yeonhwangmi' and 'Mannamiwere' transplanted into the plastic bag (0.5 mm of thickness, $W300{\times}L1,200{\times}H120mm$) culture system filled with commercial horticultural media (TKS-2, Flora Gard Co.) and cocopeat + perlite (3 : 7, 5 : 5, 7 : 3, (v/v). Nutrient solution of National Horticultural Research Station in Japan was separately irrigated $3times{\times}30minutes$ per day (10:00, 14:00 and 20:00 with timer control) by the drip irrigation. The growth of two varieties was not significantly different among four substrates in 15 days, but it was excellent in commercial horticultural media (TKS-2) and cocopeat 3 : perlite 7 (v/v) in 20 days. The growth in 'Yeonhwangmiwas' promoted than that in 'Mannami', and the upper-shoot cuttings of 'Yeonhwangmi' and 'Mannami' showed a significant vine elongation (over 10 and 2 cm, respectively) compared to the one-node cuttings. Fresh weight and dry weight in cocopeat 3 : perlite 7 (v/v) were significant increased than that in cocopeat 7 : perlite 3 (v/v). Therefore, the bag culture system filed with cocopeat 3 : perlite 7 (v/v) was successfully applied on propagation of virus-free sweetpotato seedling, and the result expected that the bag culture system could be commercialized without high financial costs for farmers.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.891-899
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• 2015

The lily symptomless virus (LSV) is the most common virus in Korean native lilies and causes various types of damage to overall plant growth. This study was carried out to investigate the elimination rate of the LSV by the in vitro scale culture (scaling) method in Korean native lilies and to test reinfection rates of the LSV under several field culture conditions of bulb production. Four Korean native lilies (Lilium dauricum, L. distichum, L. lancifolium, and L. maximowitzii) were used and their scales were cultured in vitro for micro-scale formation. The micro-scales were subcultured repeatedly using MS culture medium supplemented with 30 or $90g{\cdot}L^{-1}$ sucrose. The culture conditions were $24{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with 16 hour daylength using fluorescent lamps and maintained at $22{\pm}2^{\circ}C$. The virus-free bulblets were grown for one to three years in the greenhouse and transplanted to the field in October or March. Virus infection rates were investigated by direct tissue blotting immunobinding assays and measurement of chlorophyll and protein contents. Virus-free plants could be obtained from the 5th subculture of micro-scales in L. lancifolium and L. maximowitzii or from primary culture in L. dauricum and L. distichum. LSV-free plants were reinfected during bulb production in the field. Reinfection rates were higher at older bulb ages and under higher planting density. The plants planted in October and at inland Gyeongsan had higher infection rates than those planted in March and at coastal area Pohang. The reinfection rate of L. maximowitzii was higher than those of L. dauricum and L. lancifolium. The LSV-infected plants had lower chlorophyll contents and unchanged protein contents compared to virus-free plants.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.603-606
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• 2009

Lipoxygenase and Kunitz trypsin inhibitor protein of mature soybean [Glycine max (L.) Merr.] seed are main anti-nutritional factors in soybean seed. A new soybean cultivar, "Gaechuck#1" with the traits of black seed coat, green cotyledon, lipoxygenase2,3 and Kunitz trypsin inhibitor protein free was developed. It was selected from the population derived the cross of "Gyeongsang#1" and C242. Plants of "Gaechuck#1" have a determinate growth habit with purple flowers, brown pubescence, black seed coat, black hilum, oval leaflet shape and brown pods at maturity. Seed protein and oil content on dry weight basis have averaged 39.1% and 16.2%, respectively. It has shown resistant reaction to soybean necrosis, soybean mosaic virus, Cercospora leaf spot and blight, black root rot, pod and stem blight, and soybean pod borer. "Gaechuck#1" matured on 5-10 October with a plant height of 50 cm. The 100-seed weight of "Gaechuck#1" was 23.2g. Yield of "Gaechuck#1" was averaged 2.2 ton/ha from 2005 to 2007.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.117-128
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• 1996

A garlic (Allium sativum L.) is an important and useful vegetable such that it is not only most used for spices but also exploited for health foods and pharmaceuticals. The garlic is a kind of vegetable propagated vegetatively through cloves. Therefore, development of new varieties of garlics is possible only through selection breeding because cross breeding is impossible. (omitted)

• Journal of Plant Biotechnology
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• v.3 no.2
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• pp.59-66
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• 2001

Photoautotrophic micropropagation systems do not include sugar in the culture media. This characteristic provides advantages to scale up the micropropagation systems comparing photomixotrophic micropropagation systems. A closed, large-scale photoautotrophic micro-propagation for transplant production system has been developed at Chiba University, Japan. New concepts and technologies were adapted to produce high quality transplants at minimum usage of resources, and as scheduled. Newly developed software for production management was used to enhance the efficiency of the transplant production system. Currently, virus-free transplants of sweetpotato (Ipomoea batatas (L.) Lam.) are vegetatively propagated and produced under sterilized conditions in this system. This system can also be used for production of transplants of any other species including horticultural and woody plants with a minimum of modification.

• The Korean Journal of Mycology
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• v.20 no.3
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• pp.229-233
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• 1992

Although there were differences depending on strains and environmental conditions, virus infected oyster mushroom, Pleurotus florida showed slow growth on sawdust and rice straw substrates. Many harmful microorganisms occurred on the cultural bed of virus infected isolates. Pinhead formed too densely or too rarely sometimes. Stipes of the mushrooms were long slightly bent with small cap. The virus infected mushrooms formed branch on their stipes. The first pinheading days of the infeeted mushroom were later than that of healthy culture. The loss of fruit body yield was about 30% compared with that of virus free culture. Spores which contaminated by viruses damaged more seriously than the other source. The authors would like to call these symptoms as a new disease in oyster mushroom culture in the world.