• 제목/요약/키워드: Virtual invasion

검색결과 13건 처리시간 0.016초

Multilevel Precision-Based Rational Design of Chemical Inhibitors Targeting the Hydrophobic Cleft of Toxoplasma gondii Apical Membrane Antigen 1 (AMA1)

  • Vetrivel, Umashankar;Muralikumar, Shalini;Mahalakshmi, B;K, Lily Therese;HN, Madhavan;Alameen, Mohamed;Thirumudi, Indhuja
    • Genomics & Informatics
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    • 제14권2호
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    • pp.53-61
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    • 2016
  • Toxoplasma gondii is an intracellular Apicomplexan parasite and a causative agent of toxoplasmosis in human. It causes encephalitis, uveitis, chorioretinitis, and congenital infection. T. gondii invades the host cell by forming a moving junction (MJ) complex. This complex formation is initiated by intermolecular interactions between the two secretory parasitic proteins-namely, apical membrane antigen 1 (AMA1) and rhoptry neck protein 2 (RON2) and is critically essential for the host invasion process. By this study, we propose two potential leads, NSC95522 and NSC179676 that can efficiently target the AMA1 hydrophobic cleft, which is a hotspot for targeting MJ complex formation. The proposed leads are the result of an exhaustive conformational search-based virtual screen with multilevel precision scoring of the docking affinities. These two compounds surpassed all the precision levels of docking and also the stringent post docking and cumulative molecular dynamics evaluations. Moreover, the backbone flexibility of hotspot residues in the hydrophobic cleft, which has been previously reported to be essential for accommodative binding of RON2 to AMA1, was also highly perturbed by these compounds. Furthermore, binding free energy calculations of these two compounds also revealed a significant affinity to AMA1. Machine learning approaches also predicted these two compounds to possess more relevant activities. Hence, these two leads, NSC95522 and NSC179676, may prove to be potential inhibitors targeting AMA1-RON2 complex formation towards combating toxoplasmosis.

슈퍼히어로 코믹의 반복과 다양체적 형식에 관한 연구 (A Study on Repetition and Multiplicite of Superhero Comics)

  • 박세혁
    • 만화애니메이션 연구
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    • 통권28호
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    • pp.27-53
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    • 2012
  • 현재 다양한 플랫폼에서 슈퍼히어로 코믹을 바탕으로 한 미디어들이 쏟아져 나오고 있고 영화의 경우만 보면 다수의 슈퍼히어로 영화들이 역대 전 세계 흥행 순위 상위권에 오르는 등 높은 인기를 누리고 있다. 이러한 현상은 '슈퍼히어로의 역습'이라 표현할 수 있는 2012년 국내 극장가에서도 똑같이 재현되고 있다. 슈퍼히어로에 대한 높은 관심에도 불구하고 이들의 고향이나 다름없는 출판코믹은 아직 국내 팬들에게 생소하다. 본 논문은 출판 코믹에서 구현되는 슈퍼히어로의 다양성과 방대한 세계관에 주목하고 이러한 끊임없는 생성과 무한 반복이 슈퍼히어로의 정체성과 어떤 연관성이 있는지 존재론적 관점으로 연구한 것이다. 먼저, 2장에서 한명의 슈퍼히어로가 다수의 평행우주에 개별적으로 존재하는 슈퍼히어로 코믹의 다중 우주적 세계관을 구체적으로 설명하였다. 복수적인 한명의 슈퍼히어로와 복수의 시리즈는 내러티브의 통일성을 무너뜨리고 모순과 역설을 초래하지만 다양한 실험적 창작을 허락하기 때문에 슈퍼히어로 코믹을 생동감 있는 생성/창작의 공간으로 발전시키는데 공헌하였다. 3장에서 본격적으로 반복의 사유로 슈퍼히어로와 코믹 텍스트를 살펴본다. 슈퍼히어로의 반복은 경제적 실리의 잉여적 생산이기 이전에 창조적 역량의 반복임을 주장한다. 질 들뢰즈가 그의 초기 저서 <차이와 반복>(Diff$\acute{e}$rence et R$\acute{e}$p$\acute{e}$tition)에서 현실을 왜곡하는 일반적인 동일성을 지적하고 플라톤의 재현의 체제를 전복하고자 그가 사유한 창조적인 반복을 슈퍼히어로의 반복의 중요한 논리적 근거로 활용한다. 들뢰즈가 창조적이고 역동하는 반복과 니체의 영원회귀 하는 초인이 다르지 않다는 주장에 근거하여 슈퍼히어로가 생기 있는 차이, 동일성에 종속되지 않은 시뮬라크르의 존재라고 주장한다. 즉 슈퍼히어로는 반복이 존재의 근거며 반복되어야 슈퍼히어로로 현실화가 가능하다고 주장한다. 또 반복의 출발, 근원은 들뢰즈가 베르그송에서 주로 차용한 다양제적 잠재성(the virtual)이라는 점에 근거하여 '일자一者'적 슈퍼히어로(예로, 슈퍼맨)이 '다자多者'인 내재적 잠재성을 형성하고 포괄적이고 심층적인 반복을 통해 이전의 모든 슈퍼맨의 통합된 기억, 과거, 지속(잠재성)에서 분화된 슈퍼맨으로 탄생(현실화)한다고 주장한다. 4장에서 수적 다양성과 내적 다양체를 논하고 모든 반복이 들뢰즈의 생성과 역량의 반복으로 귀결되지 않는다는 점을 밝히기 위해 한국만화의 대표적 만화 캐릭터인 이현세 작가의 오혜성과 슈퍼맨을 비교한다.

U-마켓에서의 사용자 정보보호를 위한 매장 추천방법 (A Store Recommendation Procedure in Ubiquitous Market for User Privacy)

  • 김재경;채경희;구자철
    • Asia pacific journal of information systems
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    • 제18권3호
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    • pp.123-145
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    • 2008
  • Recently, as the information communication technology develops, the discussion regarding the ubiquitous environment is occurring in diverse perspectives. Ubiquitous environment is an environment that could transfer data through networks regardless of the physical space, virtual space, time or location. In order to realize the ubiquitous environment, the Pervasive Sensing technology that enables the recognition of users' data without the border between physical and virtual space is required. In addition, the latest and diversified technologies such as Context-Awareness technology are necessary to construct the context around the user by sharing the data accessed through the Pervasive Sensing technology and linkage technology that is to prevent information loss through the wired, wireless networking and database. Especially, Pervasive Sensing technology is taken as an essential technology that enables user oriented services by recognizing the needs of the users even before the users inquire. There are lots of characteristics of ubiquitous environment through the technologies mentioned above such as ubiquity, abundance of data, mutuality, high information density, individualization and customization. Among them, information density directs the accessible amount and quality of the information and it is stored in bulk with ensured quality through Pervasive Sensing technology. Using this, in the companies, the personalized contents(or information) providing became possible for a target customer. Most of all, there are an increasing number of researches with respect to recommender systems that provide what customers need even when the customers do not explicitly ask something for their needs. Recommender systems are well renowned for its affirmative effect that enlarges the selling opportunities and reduces the searching cost of customers since it finds and provides information according to the customers' traits and preference in advance, in a commerce environment. Recommender systems have proved its usability through several methodologies and experiments conducted upon many different fields from the mid-1990s. Most of the researches related with the recommender systems until now take the products or information of internet or mobile context as its object, but there is not enough research concerned with recommending adequate store to customers in a ubiquitous environment. It is possible to track customers' behaviors in a ubiquitous environment, the same way it is implemented in an online market space even when customers are purchasing in an offline marketplace. Unlike existing internet space, in ubiquitous environment, the interest toward the stores is increasing that provides information according to the traffic line of the customers. In other words, the same product can be purchased in several different stores and the preferred store can be different from the customers by personal preference such as traffic line between stores, location, atmosphere, quality, and price. Krulwich(1997) has developed Lifestyle Finder which recommends a product and a store by using the demographical information and purchasing information generated in the internet commerce. Also, Fano(1998) has created a Shopper's Eye which is an information proving system. The information regarding the closest store from the customers' present location is shown when the customer has sent a to-buy list, Sadeh(2003) developed MyCampus that recommends appropriate information and a store in accordance with the schedule saved in a customers' mobile. Moreover, Keegan and O'Hare(2004) came up with EasiShop that provides the suitable tore information including price, after service, and accessibility after analyzing the to-buy list and the current location of customers. However, Krulwich(1997) does not indicate the characteristics of physical space based on the online commerce context and Keegan and O'Hare(2004) only provides information about store related to a product, while Fano(1998) does not fully consider the relationship between the preference toward the stores and the store itself. The most recent research by Sedah(2003), experimented on campus by suggesting recommender systems that reflect situation and preference information besides the characteristics of the physical space. Yet, there is a potential problem since the researches are based on location and preference information of customers which is connected to the invasion of privacy. The primary beginning point of controversy is an invasion of privacy and individual information in a ubiquitous environment according to researches conducted by Al-Muhtadi(2002), Beresford and Stajano(2003), and Ren(2006). Additionally, individuals want to be left anonymous to protect their own personal information, mentioned in Srivastava(2000). Therefore, in this paper, we suggest a methodology to recommend stores in U-market on the basis of ubiquitous environment not using personal information in order to protect individual information and privacy. The main idea behind our suggested methodology is based on Feature Matrices model (FM model, Shahabi and Banaei-Kashani, 2003) that uses clusters of customers' similar transaction data, which is similar to the Collaborative Filtering. However unlike Collaborative Filtering, this methodology overcomes the problems of personal information and privacy since it is not aware of the customer, exactly who they are, The methodology is compared with single trait model(vector model) such as visitor logs, while looking at the actual improvements of the recommendation when the context information is used. It is not easy to find real U-market data, so we experimented with factual data from a real department store with context information. The recommendation procedure of U-market proposed in this paper is divided into four major phases. First phase is collecting and preprocessing data for analysis of shopping patterns of customers. The traits of shopping patterns are expressed as feature matrices of N dimension. On second phase, the similar shopping patterns are grouped into clusters and the representative pattern of each cluster is derived. The distance between shopping patterns is calculated by Projected Pure Euclidean Distance (Shahabi and Banaei-Kashani, 2003). Third phase finds a representative pattern that is similar to a target customer, and at the same time, the shopping information of the customer is traced and saved dynamically. Fourth, the next store is recommended based on the physical distance between stores of representative patterns and the present location of target customer. In this research, we have evaluated the accuracy of recommendation method based on a factual data derived from a department store. There are technological difficulties of tracking on a real-time basis so we extracted purchasing related information and we added on context information on each transaction. As a result, recommendation based on FM model that applies purchasing and context information is more stable and accurate compared to that of vector model. Additionally, we could find more precise recommendation result as more shopping information is accumulated. Realistically, because of the limitation of ubiquitous environment realization, we were not able to reflect on all different kinds of context but more explicit analysis is expected to be attainable in the future after practical system is embodied.