• Title/Summary/Keyword: VirTEX

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Mtatioal Analysis of the Role of vir-box in the Expression of the virE Gene

  • Han, Seong-Su;Sim, Woong-Seop
    • Journal of Microbiology
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    • v.37 no.3
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    • pp.175-179
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    • 1999
  • To elucidate the role of vir-box in the expression of the virE gene, the vir-box was modified by site-directed mutagenesis and tested for ${\beta}$-galactosidase activities. A, C, T T, A, C substitutions at -62, -63, and -65 positions, destroying the 5'-region of the vir-box and A T at position -55, destroying the 3'-region of the vir-box respectively, showed only 17% promoter activity. When the vir-box was modified to contain perfect dyad symmetry structure (DSR) by the substitutions T, G A, T at -60 an d-61 positions, ${\beta}$-glactosidase activity increased 302%. These results indicate that the 5' and 3'-region of vir-box as well as the imperfect DSR of the vir-box itself may play a very important role in the regulation of virE gene expression.

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Mutational Analysis of the Region between vir Box and -35 Sequence in virE Promoter of pTiA6

  • Woong Seop Sim
    • Journal of Plant Biology
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    • v.38 no.3
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    • pp.259-266
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    • 1995
  • To investigate how the dyad symmetry region (DSR) and the distance between vir box and -35 sequence of the virE promoter plays a role in virE gene expression, two mutants were constructed by base substitution and insertional mutagenesis. The base substitutional mutation, a AAlongrightarrowCG substitution at positions -39 and -40 on the DSR, showed the level of $\beta$-galactosidase activity approximately 91% of the wild type virE promoter activity. Therefore, the native structure of the DSR seems to be not essential for virE expression. The insertional mutation, constructed by inserting 8 bp ClaI linker between -49 and -50, displayed the $\beta$-galactosidase activity at 12% of the native virE promoter activity. However, this striking reduction appears to be not caused by destruction of the native DSR structure, but by shifting the vir box far from putative -35 sequence.

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Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation

  • Son, Ui-han;Dinzouna-Boutamba, Sylvatrie-Danne;Lee, Sanghyun;Yun, Hae Soo;Kim, Jung-Yeon;Joo, So-Young;Jeong, Sookwan;Rhee, Man Hee;Hong, Yeonchul;Chung, Dong-Il;Kwak, Dongmi;Goo, Youn-Kyoung
    • Parasites, Hosts and Diseases
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    • v.55 no.2
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    • pp.149-158
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    • 2017
  • Variant surface antigens (VSAs) encoded by pir families are considered to be the key proteins used by many Plasmodium spp. to escape the host immune system by antigenic variation. This attribute of VSAs is a critical issue in the development of a novel vaccine. In this regard, a population genetic study of vir genes from Plasmodium vivax was performed in the Republic of Korea (ROK). Eighty-five venous blood samples and 4 of the vir genes, namely vir 27, vir 21, vir 12, and vir 4, were selected for study. The number of segregating sites (S), number of haplotypes (H), haplotype diversity (Hd), DNA diversity (${\pi}$ and ${\Theta}_w$), and Tajima's D test value were conducted. Phylogenetic trees of each gene were constructed. The vir 21 (S=143, H=22, Hd=0.827) was the most genetically diverse gene, and the vir 4 (S=6, H=4, Hd=0.556) was the opposite one. Tajima's D values for vir 27 (1.08530, P>0.1), vir 12 (2.89007, P<0.01), and vir 21 (0.40782, P>0.1) were positive, and that of vir 4 (-1.32162, P>0.1) was negative. All phylogenetic trees showed 2 clades with no particular branching according to the geographical differences and cluster. This study is the first survey on the vir genes in ROK, providing information on the genetic level. The sample sequences from vir 4 showed a clear difference to the Sal-1 reference gene sequence, whereas they were very similar to those from Indian isolates.

Performance Analysis of Underwater Acoustic Communication Systems Using Underwater Channel Simulation Tool (수중채널 시뮬레이터를 활용한 수중음향통신 시스템 성능 분석)

  • Oh, Se-Hyun;Kim, Hyeon-Su;Kim, J.S.;Cho, Jung-Hong;Chung, Jae-Hak;Song, H.C.
    • The Journal of the Acoustical Society of Korea
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    • v.31 no.6
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    • pp.373-383
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    • 2012
  • The performance of underwater acoustic communication system is sensitive to the Doppler shift and ISI(Inter-Symbol Interference). Therefore, the simulation algorithm needs to consider time-spread due to multipath arrivals which cause the ISI, and time-varying Doppler shift along with moving source and receiver. For this purpose, VirTEX(Virtual Time series EXperiment) based on Ray model has been developed. In this paper, VirTEX is used to compare the characteristics of ocean waveguide from the experimental data and illustrate the performance. The CIR(Channel Impulse Response) that characterizes the multipath arrivals with representative time-spread due to multipath arrivals is compared between numerically simulated and experimental probe signal. Also, the communication performance analysis for BER(Bit Error Rate) is compared between numerically simulated and experimental data signal. As a result, VirTEX can be useful as a simulation tool for evaluating the performance of underwater acoustic communication system.

Rainfall Estimation Using TRMM-PR/VIRS and GMS Data (TRMM-PR/VIRS와 GMS 자료를 이용한 강수량 추정에 관한 연구)

  • 김영섭;박경원
    • Korean Journal of Remote Sensing
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    • v.18 no.6
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    • pp.319-326
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    • 2002
  • Rainfall estimation was conducted based on TRMM-PR/VIES and GMS data. AWS rainfall data were used for various validation. General procedure is as follows; 1) Z-R relationship was made by the comparison of TRMM-PR and AWS data. 2) new algorithm was developed by the estimates from Z-R equation and TBB of VIRS. 3) rainfall was estimated through the substitution of GMS data for TBB of VIRS in the newly developed algorithm. Z-R relationship based on TRMM is $Z=303R^{0.72}$ with correlation coefficient 0.57. The newly developed algorithm is shown as correlation coefficient 0.67 and RMSE 17mm/hr. New algorithm shows the underestimating tendency in case of heavy rainfall event.

Performance analysis and verification of underwater acoustic communication simulator in medium long-range multiuser environment (중장거리 다중송신채널 환경에서 수중음향통신 시뮬레이터 성능 분석 및 검증)

  • Park, Heejin;Kim, Donghyeon;Kim, J.S.;Song, Hee-Chun;Hahn, Joo Young
    • The Journal of the Acoustical Society of Korea
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    • v.37 no.6
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    • pp.451-456
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    • 2018
  • UAComm (Underwater Acoustic Communication) is an active research area, and many experiment has been performed to develop UAComm system. In this paper, we investigate the possibility of modifying and applying VirTEX (Virtual Time series EXperiment) to medium long range MIMO (Multiple-Input Multiple-Output) UAComm of about 20 km range for the analysis and performance prediction of UAComm system. Since VirTEX is a time-domain simulator, the generated time series can be used in HILS (Hardware In the Loop Simulation) to develop UAComm system. The developed package is verified through comparing with the sea-going FAF05 (Focused Acoustic Field 2005) experimental data. The developed simulator can be used to predict the performance of UAComm system, and even replace the expensive sea-going experiment.

A SPECTROSCOPIC STUDY OF THE CLOSE BINARY AG VIRGINIS (근접쌍성 AG Virginis의 분광학적 연구)

  • Kim, Ho-Il;Lee, Chung-Uk;Lee, Jae-Woo;Sohn, Mi-Rim
    • Journal of Astronomy and Space Sciences
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    • v.22 no.4
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    • pp.353-362
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    • 2005
  • We performed a new high-resolution spectroscopy of AG Vir for 4 nights from 25 March 2004 using the BOES (Bohyunsan Optical Echelle Spectrograph) attached to the 1.8-m reflector at Bohyunsan Optical Astronomy Observatory, and obtained a total of 59 spectra where all orbital phases are covered. To get the radial velocities of the binary system, both method of the CCF (Cross-Co..elation Function)and the BF (Broadening Function) were applied to the analysis of all the observed spectra. From these, the CCF could calculate the radial velocities of the primary star alone, while the BF could determine those of the primary and the secondary components. New absolute dimensions were deduced with the combination of our spectroscopic orbital elements ($K_1=90.5km/s$$K_2=258.8$) and the photometric solutions of Bell, Rainger, & Hilditch (1990): $A_1,=1.99M_\bigodot,\;M_2=0.62M_\bigodot,\;R_1=2.21R_\bigodot,\;R_2=1.36R_\bigodot,\;L_1=13.17L_\bigodot,\;and\;L_2=3.47L_\bigodot$. Our absolute parameters are larger and brighter than those derived from Bell, Rainger, & Hilditch (1990). We re-analyzed all the previous radial-velocity curves of AG Vir and, as a result, can see that its system velocity scatters largely up to ${\pm}8km/s$. However, we, at present, cannot determine this as the light-time effect due to the third body, which was suggested as a cause of the orbital period changes by Qian (2001).

Analysis of the Factors Involved in the Occurrence of Rice Stripe virus in Chungcheongbukdo in 2008 and 2009 (2008-2009년 충북지역 벼 줄무늬잎마름병 발생 요인 분석)

  • Kang, Hyo-Jung;Ahn, Ki-Su;Han, Chong-U;Jeong, Kyeong-Heon;Park, See-Jung;Ji, Jae-Jun;Kim, Jeong-Soo
    • Research in Plant Disease
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    • v.16 no.2
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    • pp.109-114
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    • 2010
  • The occurrence factors of Rice stripe virus (RSV) in Chungcheongbukdo were analyzed by investigating the viruliferous insect rate (VIR) of overwintered small brown plant hopper (SBPH), the population density of SBPH, the infection rate of natural host plants, and the occurrence rate of RSV on rice paddy fields at the 3 areas of Cheongwon, Jincheon, and Boeun in 2008 and 2009. The average VIR of overwintered SBPH was 0.0% in 2008 and 1.1% in 2009. From SBPH collected on early June in 2009, VIR was higher as 1.4% at Jincheon and 4.2% at Boeun than those of overwintered SBPH, and this higher VIR might relate stronlgy with the adult population of SBPH immigrated from China. The populations of SBPH at Cheongwon, Jincheon and Boeun in 2008 were 3.8, 7.5 and 20.8 Head/$m^2$, respectively. However, those of Cheongwon and Jincheon increased up to about two folds as 8.4 and 13.1 in 2009. No RSV was detected on the natural host plants including barley. The factors involved in RSV occurrence were affected negatively by the low VIR of overwintered SBPH, the low population of overwintered SBPH, the low infection rate of RSV on the natural host plants, and the clean cultivation environment in Chungcheongbukdo.

질량비가 작은 접촉쌍성 GR Vir

  • 오규동;김천휘
    • Bulletin of the Korean Space Science Society
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    • 2003.10a
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    • pp.21-21
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    • 2003
  • 접촉쌍성 가운데 질량비(q=m$_2$/m$_1$)가 0.2이하로 매우 작은 접촉쌍성은 그리 흔한 접촉 쌍성이 아니다. 현재까지 이러한 질량비가 작은 접촉쌍성들이 30개 정도가 알려져 있으며 이 가운데도 절대량이 잘 알려진 별들은 14개 정도에 불과하다. 이러한 원인은 이 별들에 대한 분광관측이 이루어져 있지 않기 때문이다. 따라서 이러한 접촉쌍성의 진화 상태를 보다 확실하게 이해하기 위하여 이러한 별들의 절대량을 얻는 것이 요구된다. 이번 연구에서는 Cereda at al.(1988)에 의하여 측광관측(B & V) 결과만 이루어진 후 궤도 요소가 알려져 있지 않은 GR Vir의 관측 자료와 함께 최근 Rucinski & Lu(1999)가 발표된 분광관측 결과를 이용하여 WD 프로그램을 적용하여 이 별의 궤도요소와 함께 절대량을 얻고 이로부터 이 별의 진화 상태를 조사하였다.

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The Spin-Orbit Alignment of Dark Matter Halo Pairs: Dependence on the Halo Mass and Environment

  • An, Sung-Ho;Yoon, Suk-Jin
    • The Bulletin of The Korean Astronomical Society
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    • v.44 no.1
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    • pp.35.1-35.1
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    • 2019
  • We present a statistical analysis on the spin-orbit alignment of dark matter halo pairs in cosmological simulations. The alignment is defined as the angular concurrence between the halo spin vector (${\vec{S}}$) and the orbital angular momentum vector (${\vec{L}}$) of the major companion. We identify interacting halo pairs with the mass ratios from 1:1 to 1:3, with the halo masses of 10.8 < $Log(M_{halo}/M_{sun}$) < 13.0, and with the separations smaller than a sum of their virial radii ($R_{12}<R_{1,vir}+R_{2,vir}$). Based on the total energy ($E_{12}$), the pairs are classified into flybys ($E_{12}$ > 0) and mergers ($E_{12}{\leq}0$). By measuring the angle (${\theta}_{SL}$) between ${\vec{S}}$ and ${\vec{L}}$, we confirm a strong spin-orbit alignment signal such that the halo spin is preferentially aligned with the orbital angular momentum of the major companion. We find that the signal of the spin-orbit alignment for the flyby is weaker than that for the merger. We also find an unexpected excess signal of the spin-orbit alignment at $cos{\theta}_{SL}{\sim}0.25$. Both the strength of the spin-orbit alignment and the degree of the excess depend only on the environment. We conclude that the halo spin is determined by the accretion in a preferred direction set by the ambient environment.

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