• Korean Journal of Pharmacognosy
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• v.52 no.1
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• pp.34-40
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• 2021

Proliferation and maintain of dermal papilla during progression of hair-cycle are crucial to the duration of anagen and regulated by diverse signaling pathway such as PI3K/Akt/Wnt/β-catenin pathway. In this study, we investigated the effects and mechanisms of Viola verecunda on dermal papilla cells. Treatment of dermal papilla cells with whole plant extract of V. verecunda resulted in cell proliferation, which was accompanied by up-regulation of cyclin D1, phospho (ser780)-pRB and cdc2 p34, and down-regulation of p27kip1. V. verecunda extract also promoted the levels of phospho (ser473)-Akt and phospho (ser780)-pRB in a time-dependent manner. Inhibition of PI3K/Akt by Wortmannin suppressed progression of cell-cycle, thereby attenuated the increases in proliferation of dermal papilla cells by V. verecunda extract. We further investigated Wnt/β-catenin pathway with respect to the effects of V. verecunda extract on the proliferation of dermal papilla cells. Treatment with V. verecunda extract results in up-regulation of Wnt/β-catenin proteins such as phospho (ser9)-GSKβ, phospho (ser552)-β-catenin and phospho (ser675)-β-catenin. In addition, Wortmannin abrogated V. verecunda extract mediated up-regulation of cdc2 p34 and down-regulation of p27kip1. These finding reveal that the proliferative effect of V. verecunda mediated by alteration of cell-cycle via activating PI3K/Akt/Wnt pathway in dermal papilla cells.

• Korean Journal of Plant Resources
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• v.19 no.6
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• pp.716-720
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• 2006

The genetic diversity among the genus Viola was evaluated using the random amplified polymorphic DNA (RAPD) method. A total of 142 distinct amplification fragments by 18 random primers were scored to perform the cluster analysis with UPGMA. Viola species from the subsection Patellares were clustered into group I to IV. The groups from I to IV were consistent with its morphological taxonomy, series Pinnatae, Chinensis, Variegatae, and Patellares in the subsection Patellares, respectively. Even though V. albida and V. albida var. takahasii were classified in Chinensis, they were assigned into group I. The cluster analysis separated other subsections from Patellares in the section Nomimium. Interestingly, V. verecunda and V. grypoceras in subsections Biobatae and Trigonocarpae, respectively, were clustered into group C with a high similarity coefficient. Therefore, RAPD analysis can be used for providing an alternative classification system to identify genotypes and morphological characters of Viola species.

• Korean Journal of Plant Resources
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• v.19 no.6
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• pp.700-705
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• 2006

Present studies were performed to determine the physiology of seed germination in Viola species native to Korea. Twelve species, 1 variety and 1 form were collected, classified and used as materials: V. collina, V. blandaefomis, V. rosii, V. chaerophylloides, V. phalacrocarpa, V. patrinii, V. mandshurica, V. mandshurica for. albescence, V. seoulensis, V. yedoensis, V. keiskei, V. variegata, V. variegata var. chinensis, and V. verecunda. V. tricolor 'Helen Mount' was also used to compare wild with cultivated species. In order to investigate the effect of temperature on seed germination, seeds stored at $4{\pm}2^{\circ}C$ for 10 months or 4 years were incubated at 10, 15, 20, $25^{\circ}C$ under 16h illumination with 4 replicates per treatment. Seeds which had not germinated at $10^{\circ}C$ were transferred to $30^{\circ}C$ to assess the effect of temperature change in germination. Germination percent and the days of first, 40% and 80% germination were assessed. Capability of seed germination varied with taxon; Species belonging to subsection Patellares had high ability of germination, compared to species in the other subsections, and series Chinensis was the best among subsection Patellares. Species capable of high germination germinated in all temperatures with reasonably high germination rate, but the other species responded sensitively to temperature with different germination patterns. Higher the temperature, shorter the incubation time required for first, 40% and 80% of germination. Therefore, high temperature was effective in almost all species, not only for inducing high rate of germination but also the uniformity of germination. Temperature change from $10^{\circ}C\;to\;30^{\circ}C$ had a positive effect on seed germination.

• Korean Journal Plant Pathology
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• v.14 no.1
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• pp.52-61
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• 1998

This paper is the fifth report about the fungal diseases of economic resource plants observed newly in Korea. It contains short descriptions on symptoms, occurrence conditions, pathogens, and some phytopathological notes for each of 10 fungal plant diseases. They are identified as leaf spot of Adenophora triphylla var. japonica by Septoria lengyelii, leaf spot of Calystegia soldanella by S. convolvuli, leaf spot of Campanula punctata by S. campanulae, leaf spot of Codonopsis lanceolata by S. codonopsidis, leaf spot of Geum japonicum by s. gei, black spot of Oenanthe javanica by s. oenanthes, leaf spot of Oenothera odorata by S. oenotherae, angular leaf spot of Rehmannia glutinosa by S. digitalis, brown spot of Rubus crataegifolius by s. rubi, and leaf spot of Viola verecunda by S. violae-palustris, respectively.

• Journal of Korean Society of Forest Science
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• v.109 no.2
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• pp.124-135
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• 2020

Alder (Alnus japonica) forests are representative of the wetland in East Asia, including Korea. In the past, alder forests were relatively common in various habitats such as mountains, riversides, back marshes, and alluvial plains. However, this plant community has recently become rare due to increasingly arid habitats and the influence of various land uses. In this study, we identify the synecological characteristics of alder (A. japonica) forests distributed naturally in the mountainous wetlands of Korea and provide basic data for their systematic conservation and management in the future. Based on vegetation survey data collected from 66 alder forests, community types were classified using the methods of the Zürich-Montpellier School of Phytosociology and two-way indicator species analysis. There were eight community types: Styrax obassia, Weigela subsessilis-Fraxinus mandschurica, Spiraea fritschiana, Viola verecunda, Impatiens textori-Spiraea salicifolia, Glyceria leptolepis, Molinia japonica, and Lindera obtusiloba-Quercus acutissima. These community types constituted a vegetation unit hierarchy of two communities, four subcommunities, and eight variants. In addition, the ecological characteristics of each community type were compared (including total coverage per 100 square meter, importance value index, constancy class, life-form composition, diversity indices, community similarity coefficient, and indicator species).

• The Korean Journal of Ecology
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• v.22 no.5
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• pp.277-285
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• 1999

To develop a better restoration technique for altering urban planted forest to more natural forest, the changes of flora, vegetation structure and woody plant growth in the early stage after lumbering only Populus albaglandulosa of the tree layer were studied in Chungdam Park, Chungdam-dong, Kangnam-gu, Seoul for two years (from 1997 to 1998). The results were as follow. First, in the year after lumbering, the species changes occurred not in woody plants but in herbs, which increased by 44%. Three species including Viola verecunda disappeared, and 15 species including ruderals such as Metaplexis japonica and introduced species such as Erechtites hieracifolia appeared newly. Second, lumbering caused the coverages of Quercus aliena, Q. mongolica, Q. acutissima and Sorbus alnifolia in the subtree, or shrub layer, to be increased abruptly. The coverage of Lespedeza bicolor was affected by neighboring plants. Third, in the herb layer the coverage of Artemisia keiskeana was conspicuously increased but those of the others were not. Fourth, by ridding the upper layer canopy, Quercus' growth rates were increased highly. Rates of DBH growth of Q. aliena, Q. mongolica and Q. dentata were increased to 53.0%, 22.9% and 8% in the experimental area, and 23.1%, 8.3% and 6.1% in the control area, respectively, during two growing seasons (from May 11, 1997 to October 27, 1998). The ratios of twig biomasses of the previous year to the next year were 100:565 in Q. aliena, 100:197 in Q. mongolica and 100:644 in Q. dentata in the experimental area. There were also growth ratios of 100:117 in Q. aliena, 100:100 in Q. mongolica and 100:42 in Q. dentata in the control area, respectively. The growth rate increases of Q. aliena and Q. dentata were thus conspicuous in twig rather than in trunk, but that of Q. mongolica was vice versa.