• The Journal of the Korean Society for Microbiology
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• v.22 no.1
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• pp.61-70
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• 1987

Authors have isolated phages of V. parahaemolyticus from shellfish and investigated some of their characteristics. The results obtained were as follows: Twenty-three phage strains(9.2%) out of 250 specimens were isolated. Plaques of phages were small, clear or turbid and $0.5{\sim}1.5mm$ in diameter. The electron micrographs of K3 phages showed two morphology; one was a hexagonal head about 105nm with a tail about 12nm, the other was a hexagonnal head about 60nm with a tail about 25nm. The host ranges of pahges were limited to V. parahaemolyticus strains and there appeared to be no relationship between the K serotypes of V. parahaemolyticus strains and the host ranges of the phage isolates. The adsorption rate of phages were more than 80% for $10{\sim}15$ minutes, the inactivation rate at $60^{\circ}C$ was more than 99% for $40{\sim}45$ minutes. The pH stability range was between 6.0 and 8.0. The inactivation rate of phages by UV irradiation was more than 99% for $45{\sim}75$ seconds.

• The Journal of the Korean Society for Microbiology
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• v.34 no.5
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• pp.423-433
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• 1999

A novel bacteriophage, designated as VPP97, that infects the strains of Vibiro parahaemolyticus (hallophilic, Gram-negative bacterium) isolated most commonly from marine environments, has been discovered, and several of its properties have been determined. The plaques were clear and sized $0.6{\sim}1.0\;mm$ in diameter. The virion forms a single band on 70% sucrose gradient and ${\rho}1.50$ CsCl gradient by sucrose gradient centrifugation and CsCl gradient centrifugation respectively. It has a hexagonal head and a relatively long tail, as shown by electron microscopy. Vibrio alginolyticus, Vibrio fluvialis and Vibrio furnissii were also sensitive to this phage. It was almost totally inactivated at $70^{\circ}C$ and at pH below 5 or over 10. The nucleic acid of VPP97 is composed of DNA. The VPP97 had 9 specific structural proteins sized between 21.5 kDa and 97.4 kDa on SDS-PAGE. When V. parahaemolyticus cultures were treated with either phage VPP97 or one of the several antibiotics for 2 hours, the viable number of V. parahaemolyticus treated with the phage VPP97 is lower than that treated with chloramphenicol, erythromycin or penicillin, but not lower than that treated with tetracycline. Mice that have responded to the phage treatment revealed the lower numbers of V. parahaemolyticus in small intestine and less damage on small intestine compared to the untreated mice. Therefore, we suggest that the phage treatment appears effective to the infection by V. parahaemolyticus.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.1 no.2
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• pp.99-103
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• 1997

The bacteriophages lytic for Vibrio furnissi, Vibrio fluvialis and Vibrio parahaemolyticus were isolated from fish gills and shellfish. Nucleic acid of bacteriophages was prepared and restriction endonuclease profile was compared. All isolates contained deoxyribonucleic acid. V. furnissi bacteriophage from fish gills showed 2 bands with Bgl II, 1 with Pst, 3 with Hind III, I with Bam HI and 2 with EcoR 1. V fluvialis phage represented 7 fragments with Bgl II, 1 with Pst, 4 with Hind III, and 2 with EcoR 1. V. parahamolyticus produced 13 sites with Hind III and 4 sites with EcoR 1. The fragment types were varied depending on the phage isolation. All three phages were digested with Hind III and EcoR I with different sizes. V. furnissi phage were digested with 5 different restriction enzymes.