• The Journal of the Korean Society for Microbiology
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• v.13 no.1
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• pp.37-42
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• 1978

Identification and serotyping for 21 strains of V. parahaemolyticus isolated from diarrheal patients in Japan and Indonesia in 1977 were carried out. The results were as follows: 1. The 21 strains were divided into 5 serovars including O1:K56, O4:K4, O4:K8, O4:K12 and O5:K15. 2. Author's experiences on the isolates in Korea also show that there is no particular difference in the distribution pattern of serovars of V. parahaemolyticus among Korea, Japan and Indonesia. In these respects, there was no geographical difference in these countries.

• Journal of Environmental Health Sciences
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• v.10 no.2
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• pp.53-59
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• 1984

V. parahaemolyticus is a species of gram- negative asperogenous rod which gives rise to gastroenteritis in man and widely distributed in Korea. In this study, the distribution of V. parahaemolyticus among sea water and various marine products in Inchcon during the period of July 1984 to August 1984 was studied. The following results were obtained: 1. V. parahacmolyticus were isolated from 16(15.4%) of 104 specimens of sea water and various marine products. 8 strains(11.0%) were from fish and marine products 3 (25.0%) from aquarium watar, 3 from chopping board 1 from sea water and 1 from sea water in basin. 2. Among the 14 strains tested, 10 were Kanagawa phenomenon positive and 4 were negative. 3. 16 strains were tested for K antigens and 3 were found to be $K_{17}$, 1 each of $K_{22}, K_{25}, K_{34}$ and $K_{57}$. 7 strains were not agglutinated with any K-type multiserum.

• Journal of Food Hygiene and Safety
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• v.22 no.2
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• pp.127-131
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• 2007

This study evaluated the bactericidal effect of 10 sanitizers and disinfectants such as ethanol (75 and 95%), iodine (15 and 25 ppm), chlorine (100 and 200 ppm), quaternary ammonium, acid, hydrogen peroxide, and peroxide acetic acid against V. parahaemolyticus. Ten strains of V. parahaemolyticus isolated from Korean foods and 4 strains of standard V. parahaemolyticus were compared for efficacies of various sanitizers and disinfectants by EN 1276 method based on quantitative suspension test. Ethanol (75 and 95%), 25 ppm of iodine, 100 ppm of quaternary ammonium, 145 ppm of hydrogen peroxide and acid showed more than $5log_{10}CFU/mL$ reduction in both clean and dirty conditions. Tests result of chlorine (100 ppm) showed more than $5log_{10}CFU/mL$ reduction in clean condition. Iodine (15 ppm) showed more than $5log_{10}CFU/mL$ reduction except 4 isolated and 1 standard V. parahaemolyticus in clean condition. iodine (15 ppm) also showed under $5log_{10}CFU/mL$ reduction $(0.93{\sim}3.73log_{10}CFU/mL)$ in dirty condition. Eleven hundred ppm of hydrogen peroxide was evaluated as weak sanitizer and disinfectant due to their $0.99{\sim}4.79log_{10}CFU/mL$ reduction on both clean and dirty conditions. Consequently, ethanol, iodine (25 ppm), chlorine (200 ppm), quaternary ammonium, acid and peroxide acetic acid were thought to be effective sanitizer and disinfectant against V. parahaemolyticus.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.1
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• pp.17-25
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• 1996

The results identified for bacterial flora 174, Vibrio spp.132, and coliform group 183 strains isolated from the areas of last China Sea and Yosu coastal sea during from August 6th. to 14th. 1992 were as follow: 40 strains among the 74 strains of bacteria flora isolated from fast China sea area were Pseudomonas spp.$(54\%)$ and 60 strains among the 100 strains isolated from Yosu sea area were Enterobacteriaceae $(60\%)$. Four strains were Vibrio alginoliticus and one strain of V, parahaemolyticus among 5 strains of genus Vibrio isolated from last China Sea. While 54 strains were V. alginolyticus $(43\%)$ and V, parahaemolyticus $(17\%)$ among 127 strains genus Vibrio isolated from Yosu coastal sea area. Seventy nine strains among the 156 strains of coliform group isolated from Vosu sea area were Escherichia coli I $(51\%)$ and each one strain Citrobacter freundii I and II. 3 strains among 27 strains isolated from last China sea area were E. coli$(11\%)$ and 1 strain of C. freundii I. Coliform group was grouped into 16 types by IMViC system, $44^{\circ}C$, gelatin liquefaction test.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1593-1601
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• 2017

Vibrio species are generally recognized as pathogens predominant in seafood along coastal areas. The food industry has sought to develop efficient microbial detection methods. Owing to the limits of conventional methods, this study aimed to establish a rapid identification method for Vibrio isolated from Korea, based on matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Four different preparation procedures were compared to determine the appropriate means to pretreat Vibrio species, using 17 isolates and five reference strains. Extended direct transfer and full formic acid extraction methods using bacterial colonies on agar plates revealed very low identification rates. Formic acid and trifluoroacetic acid (TFA) extractions using bacterial broth cultures were also performed. All Vibrio isolates and reference strains prepared by TFA extraction were successfully identified to the species level (17/22, 77.3%) and to the genus level (5/22, 22.7%). Thus, TFA extraction was considered the most appropriate method to pretreat Vibrio species for MALDI-TOF MS. The remaining 33 isolates and two reference strains were prepared by TFA extraction and analyzed by MALDI-TOF MS. Overall, 50 isolates were identified to the species level (40/50, 80%) and to the genus level (10/50, 20%). All isolates were identified as 43 V. alginolyticus, six V. parahaemolyticus, and one V. vulnificus species. V. alginolyticus and V. parahaemolyticus were isolated from fish offal (87.5% and 12.5%, respectively), seawater (91.3%, 8.7%), and shellfish (62.5%, 37.5%), whereas V. alginolyticus and V. vulnificus were isolated from sediment (90.9% and 9.1%, respectively). This study established a reliable system of MALDI-TOF MS preparation and analysis for Vibrio identification.

• The Journal of the Korean Society for Microbiology
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• v.35 no.5_6
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• pp.371-371
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• 2000

• Proceedings of the Korean Society of Life Science Conference
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• 2002.03a
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• pp.74-74
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• 2002

• Journal of Life Science
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• v.10 no.5
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• pp.524-528
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• 2000

Antimicrobial and antioxidative effects of Alismatis Rhizoma which has been used as a galenic for diuretic and antiphlogistic were investigated. In a paper disc test, water extract of Alismatis Rhizoma showed antimicrobial activities against almost all of the microbial species tested, while their activities of methanol and ethanol extract was low than the activity of water extract, and the antimicrobial activity of water extract was most prominent against Vibrio parahaemolyticus. Antioxidative activity against linoleic acid was not observed in methanol extract. In contrast to methanol and ethnol and ethanol extract, water extract showed a antioxidative activity against linoleic acid. However, the antioxidative of water extract was lower than that of 0.1% BHT.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.196-200
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• 2002

Compositions of four kinds of products inhibit aging and protect many kinds of disease through eliminating. These compositions inhibit growth of harmful bacteria. such as Bacteroides fragilis. Clostridium perfringens. Staphylococcus aureus, Listeria monocytogenes. Vibrio parahaemolyticus etc. Bacteroides fragilis was controlled by mixing SCUTELLARIA BAICALENSIS GEORGE+CRATAEGI FRUCTUS+PAEONIA JAPONICA+SCHIZANDRAE FRUCTUS. SCUTELLARIA BAICALENSIS GEORGE+CRATAEGI FRUCTUS+PAEONIA JAPONICA+CORNI FRUCTUS. etc. and Clostridium perfringens was SCUTELLARIA BAICALENSIS GEORGE+PHELLODENDRI CORTEX+EPIMEDII HERBA+ASTRAGALI RADIX. SCUTELLARIA BAICALENSIS GEORGE+EPIMEDII HERBA +ASTRAGALI RADIX+GLYCYRRHIZAE RADIX.ste. Listeria monocytogenes was NELUMBO NUCLFERA GAERTNER+SCUTELLARIA BAICALENSIS GEORGE+COPTIDIS RHIZOMA+PAEONIA JAPONICA, NELUMBO NUCLFERA GAERTNER+SCUTELLARIA BAICALENSIS GEORGE+COPTIDIS RHIZOMAtSOPHORAE FLOS. etc. and Vibrio parahaemolyticus was ACANTHODANACIS CORTEX +ASTRAGALI RADIX+PHELLODENDRI CORTEX+COPTIDIS RHIZOMA ACANTHODANACIS CORTEX +ASTRAGALI RADIX + PHELLODENDRI CORTEX+PAEONIA JAPONICA. We expected it as a functional food economical and easy type of taking.

• Journal of Life Science
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• v.16 no.4
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• pp.640-646
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• 2006

The present investigation was carried out particularly to optimize the extraction process of Poncirus trifoliata and Prunus mume to develop a functional foodstuff having antibacterial activity against Vibrio parahaemolyticus. The extracts were prepared from the fruits under various conditions, and then optimum extraction conditions were decided in case maximal antibacterial activity was obtained. In extraction process by using hot water, the best antibacterial activity of Poncirus trifoliata was achieved at $80{\sim}100^{\circ}C$ for 30 min, while that of Prunus mume was achieved at $100^{\circ}C$ for 30 min, as showing 14.8 and 16.6 mm of inhibition diameter, respectively. The extraction process with addition of NaOH increased the activity of Prunus mume, but addition of $K_2CO_3$ and $NaHCO_3$ drastically decreased the activity. In the processes at high temperature up to $120^{\circ}C$ by using pressure extractor and with various concentrations of NaOH, the maximum activity was observed in the extract of Prunus mume at $120^{\circ}C$ for 30 min with 0.05 N NaOH. The fermented ethanol extract of the fruits showed less activity than those of the extracts with water and NaOH solution.