• Asian Pacific Journal of Cancer Prevention
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• v.16 no.17
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• pp.7409-7414
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• 2015

Loop-mediated isothermal amplification (LAMP) developed by Notomi et al. (2000) has made it possible to amplify DNA with high specificity, efficiency and rapidity under isothermal conditions. The ultimate products of LAMP are stem-loop structures with several inverted repeats of the target sequence and cauliflower-like patterns with multiple loops shaped by annealing between every other inverted repeats of the amplified target in the similar strand. Because the amplification process in LAMP is achieved by using four to six distinct primers, it is expected to amplify the target region with high selectivity. However, evaluation of reaction accuracy or quantitative inspection make it necessary to append other procedures to scrutinize the amplified products. Hitherto, various techniques such as turbidity assessment in the reaction vessel, post-reaction agarose gel electrophoresis, use of intercalating fluorescent dyes, real-time turbidimetry, addition of cationic polymers to the reaction mixture, polyacrylamide gel-based microchambers, lateral flow dipsticks, fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays and nanoparticle-based colorimetric tests have been utilized for this purpose. In this paper, we reviewed the best-known techniques for evaluation of LAMP amplicons and their applications in molecular biology beside their advantages and deficiencies. Regarding the properties of each technique, the development of innovative prompt, cost-effective and precise molecular detection methods for application in the broad field of cancer research may be feasible.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2013.04a
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• pp.589-595
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• 2013

A reactor vessel internals comprehensive vibration assessment program(RVI CVAP) of an advanced power reactor 1400(APR1400) is being verified on the integrity of RVI for the design life of the plant by performing the non-prototype category-2 type on the US Nuclear Regulatory Commission Guide(NRC RG) 1.20, for which consists of a vibration and stress analysis program, a limited vibration measurement program, an inspection program, and the correlation of these programs. The aim of this paper is to describe the plan for the vibration measurement, test and acceptance criteria portion, and documentation and results of the APR1400 RVI CVAP. We will conduct the limited vibration measurement program of the APR1400 RVI CVAP according to the measurement plan and the vibration measurement testing in this paper.

• Journal of the Korean Society for Nondestructive Testing
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• v.30 no.4
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• pp.338-344
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• 2010

A double rotation C-scanning system and a software program Under-Sodium MultiVIEW have been developed for the under-sodium viewing of a reactor core and in-vessel structures of a sodium-cooled fast reactor KALIMER-600. Double rotation C-scanning system has been designed and manufactured by the reproduction of double rotation plug of a reactor head in KALIMER-600. Hardware system which consists of a double rotating scanner, ultrasonic waveguide sensors, a high power ultrasonic pulser-receiver, a scanner driving module and a multi channel A/D board have been constructed. The functions of scanner control, image mapping and signal processing of Under-Sodium MultiVIEW program have been implemented by using a LabVIEW graphical programming language. The performance of Under-Sodium MultiVIEW program was verified by a double rotation C-scanning test in water.

• Journal of Radiation Protection and Research
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• v.28 no.2
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• pp.127-135
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• 2003

The primary contributors to the total occupational radiation exposure in operating nuclear power plants are operation and maintenance activities doting refueling outages. The Advanced Power Reactor 1400 (APR1400) includes a number of design improvements and plans to utilize advanced maintenance methods and robotics to minimize the annual collective dose. The major radiation exposure reduction features implemented in APR1400 are a permanent refueling pool seal, quick opening transfer tube blind flange, improved hydrogen peroxide injection at shutdown, improved permanent steam generator work platforms, and more effective temporary shielding. The estimated average annual occupational radiation exposure for APR1400 based on the reference plant experience and an engineering judgment is determined to be in the order of 0.4 man-Sv, which is well within the design goal of 1 man-Sv. The basis of this average annual occupational radiation exposure estimation is an eighteen (18) month fuel cycle with maintenance performed to steam generators and reactor coolant pumps during refueling outage. The outage duration is assumed to be 28 days. The outage work is to be performed on a 24 hour per day basis, seven (7) days a week with overlapping twelve (12) hour work shifts. The occupational radiation exposure for APR1400 is also determined by an alternate method which consists of estimating radiation exposures expected for the major activities during the refueling outage. The major outage activities that cause the majority of the total radiation exposure during refueling outage such as fuel handling, reactor coolant pump maintenance, steam generator inspection and maintenance, reactor vessel head area maintenance, decontamination, and ICI & instrumentation maintenance activities are evaluated at a task level. The calculated value using this method is in close agreement with the value of 0.4 man-Sv, that has been determined based on the experience aid engineering judgement. Therefore, with the As Low As Reasonably Achievable (ALARA) advanced design features incorporated in the design, APR1400 design is to meet its design goal with sufficient margin, that is, more than a factor of two (2), if operated on art eighteen (18) month fuel cycle.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.3
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• pp.554-562
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• 2004

It was recommended for all ship engaged in international voyages to carry the Satellite Emergency position Indicating Radio Beacon according to Global Maritime Distress ＆ Safety System but also it is tending to be diffused to require the satellite EPIRB installed in small vessel such as a fishery boat by domestic regulations of each countries. Otherwise the domestic regulation can't evaluate exactly the compatibility with the satellite system and the performance of the equipment itself required by international regulation. And even if it is required the performing of the performance inspection in accordance with international regulation, it is difficult to grasp the characteristics of equipment by a generalized measuring equipment. Hence this study performed the measurement for the matters related with an wave forms, modulation characteristics, frequency and the power, etc through the design and implementation of the test bench capable of ensuring most of test items regulated by international regulation. Through this study, it has been paved the way being capable of preparation of the standard regarding to transmission repetition period, phase variation and frequency stability related with the system compatibility and in conclusion, it is enable to improve the performance of a main distress communication equipment directly connected with the life of a survivor.

• Korean journal of applied entomology
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• v.59 no.2
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• pp.117-127
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• 2020

We monitored on the hitchhiker insect pests from a total of 112 foreign vessels entering into Korea during 109 days from 1. Jun. to 17. Sep. in 2018. As the results, a total of 336 samples of the hitchhiker insects were collected using simply collecting method by hands, one of interception method and identified as 159 species of 47 families under nine orders on the basis of integrative species identification. Among them, 14 species of nine families under three orders were detected as undistributed species in South Korea. We reported on the 14 undistributed species in South Korea with the data sheets for the risk assessments on invasive or invasive likelihood species. Also, we suggested and used the term 'not-distributed species' for avoiding confusion with 'unrecorded species', 'unreported species', or 'new reported species'.

• Composites Research
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• v.23 no.6
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• pp.7-13
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• 2010

Type3 cylinder is a composite pressure vessel fully over-wrapped with carbon/epoxy composite layers over an aluminum liner, which is the most ideal and safe high pressure gas container for CNG vehicles due to the lightweight and the leakage-before-burst characteristics. During service in CNG vehicle, if a fiber cut damage occurs in outer composite layers, it can degrade structural performance, reducing cycling life from the original design life. In this study, finite element modeling and analysis technique for the composite cylinder with fiber-cut crack damage is presented. Because FE analysis of type3 cylinder is path dependant due to plastic deformation of aluminum liner in autofrettage process, method to introduce a crack into FE model affect analysis result. A crack should be introduced after autofrettage in analysis step considering real circumstances where crack occurs during usage in service. For realistic simulation of this situation, FE modeling and analysis technique introducing a crack in the middle of analysis step is presented and the results are compared with usual FE analysis which has initial crack in the model from the beginning of analysis. Proposed analysis technique can be used effectively in the evaluation of influence of damage on composite layers of type3 cylinder and establish inspection criteria of composite cylinder in service.

• Korean journal of applied entomology
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• v.60 no.3
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• pp.269-276
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• 2021

During 15 months from 13. Jul. 2018 to 29. Oct. 2019, we monitored on the hitchhiker insect pests from a total of 306 foreign vessels entering into Korea. As the results, a total of 805 samples of the hitchhiker insects were collected using simply collecting method by hands and identified as 379 species of 78 families under 12 orders on the basis of integrative species identification. Among them, 42 species (67 individuals) of 21 families under seven orders were detected as not-distributed species in South Korea. Of the 42 not-distributed species, seven species were multiply detected in 2018 and 2019, and two species, Erthesina fullo (Pentatomidae, Hemiptera) and Tessaratoma papilosa (Tessaratomidae, Hemiptera) were discovered as regulated insect pest. Therefore, we reported on the 42 not-distributed species in South Korea with the data sheets for the establishment of a monitoring plan on the not-distributed species and the risk assessments on invasive or invasive likelihood species.

• Journal of Navigation and Port Research
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• v.45 no.1
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• pp.9-15
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• 2021

According to IMO, MASS is defined as a vessel operated at various levels independent of human interference. The safety navigation support service for MASS is designed to improve the safety and efficiency of MASS by developing public services on shore for ship arrivals/departures and for cargo handling. The safety navigation support service consists of a total of six types of services: autonomous operation, berthing/unberthing/mooring, cargo handling and ship arrival/departure service, PSC inspection, condition monitoring, and accident response support services. In order to support accident response service, the relative importance of a bridge navigational equipment was assessed by stratifying the navigation system to provide safe and efficient support services by objective judgment through specific and quantitative methods using AHP, one of decision-making methods used by an expert group. The survey was conducted by dividing the bridge navigational equipment into depth, location, and speed information. As a result of applying the AHP method, the importance of depth, location, and speed information was assessed. The relative importance of each equipment for providing location information was also assessed in order of Radar, DGPS, ECDIS, Gyro compass, Autopilot, and AIS. This was similar to survey results on the utilization of each operator's preference and its impact on marine accidents.

• Journal of Sasang Constitutional Medicine
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• v.10 no.2
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• pp.351-429
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• 1998

The Purpose of Study 1. Inspection of clinical application on TCD to CVA 2. Objective Comparement and analysis about treatment effect of Western-Medicine, Korean Medicine, Cooperative consultation of Korean and Western medicice for CVA The Subject of Study We intended for the eighty six patient of CVA who had been treated in the Oriental Medical Hospital at Dong Eui Medical Center from 1997. 8. I to 1998. 7. 31 1. View of CT, MRI : the patient of Cb infarction 2. Attack Time : The patient who coming hospital falling ill within the early one week The method of study 1. Treat four group of Korean medicine, Constitution medicine, Western medicine, cooperative consultation of Korean medicine and Western medicine. 2. Application of TCD Check the result for three times, immediatly after the attack, two months later, four months later 3. Comparative analysis of each treatment effect by clinical symptoms and pathologic examination 4. The Judgement of the patient The Result From 8/1/1997 to 7/31/1998, We have the following result by clinical analysis intended for CVA 86 patients who had been treated in the Oriental Medical Hospital at Dong Eui Medical Center from 1997. 8. 1 to 1998. 7. 31 in 1. Analysis according to Age The first stage of thirties, forties, seventies is heavier than forties, fifties in improvement and Index of improvement of symptom 2. Analysis according to sex We have no special relation in an average of symptom and improvement, Index improvement 3. Analysis according to Family History We have the better result in first stage and improvement, index improvement when no family history. 4. Analysis according to Past History We have no special relation in past history like hypertension, DM, heart problem 5. Analysis devided two group, above group and under group on the basis of the average in first stage of all patient. We have the better result when the first stage is light, that the first score of barthel index and CNS is high. 6. Analysis of the effect of treatment about Korean medical treatment, Western medical treatment, cooperative treatment. In this study, the highest group of rate of treatment at four contrast groups (Korean medicine, Constitution medicine, Western medicine, cooperative treatment according to dyagnosis and range of treatment was the patient group of doing dyagnosis and method of treatment based on constitution medicine theory. This is that of doing demostation, A-Tx, po-herb-medication according to dyagnosis and treat method of constitution of Lee Je-ma In case of left, the case of dyagnosis any disease according to doctor view but, normal in TCDwas 22-beginning of attack, 20- two weeks later, 11 case-four weeks later in case of right, 15-beginning of attack, 12-two weeks later, 9 case four weeks later. So left vessel compares to right vessel is more interference, in fact more than a 1/2 of the patients of MCA disease can't do dyagnosis. In rate of imparement, the state of pacient improved but there isn't the improved case of result in TCD. 7. In TCD dyagnosis, between the case of inconsus the doctor view specially MCA in brain blood vessel is in large numbers and in total 86's patient, impossible case of dyagnosis according to interferiance of temporal is 21 case. 7. Result study about application of Kreaan medical treatment 1) The impossible patient of observation MCA blood vescular for interference temporal bone happened in large numbers. 2) There is the case having difference result to CT,MRI, MRA result. 3) Because individual difference is large, excluding to ananalogy of symptom. This is normal numerical value that has possibility of being checked as abnormal numerical value 4) there are a lot of cases that the speed of normal part is as similarly measured as that of abnormal part. It means that we cannot judge the disease by this measure 5) It is rare that this measure represent degree of improvement in patient's condition of disease. When we observe patient's condition become better, but we have no case that the result of TCD test better. 6) The result could be appear differently by the technique of the tester or by the experience of the tester 7) In the TCD test, abnormal symptoms is checked at 0 week, but at 2th week, normal symptoms is checked, again at 4th week abnormal is checked. According to the above result, CVA diagnosis is difficult only with TCD, as it appear in diagnosis error check which is suggested in the problem connected to project, for the aged persons who have the worst hardening of part of the cranium (1998. 5. 26 77 of 83 patients is 50s) there is a lot of cases that the measurement is impossible by TCD and the correction of measurement numerical value is decreased, as the age of cerebral infarction is high, TCD is inappropriate to diagnosis equipment through this study.