• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.167-176
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• 1999

Shoot morphological characteristics were affected by plant population densities in alfalfa. Shoots per plant, yield per shoot, yield per plant, and stem diameter increased with decreasing alfalfa population density. Winter survival was maximized at a plant population of $45plants\;m^{-2}$ or less. The grams of TNC per root increased as the stand density decreased. The lowest LT 50 occurred at the lowest stand density ($16plants\;m^{-2}$ having 25 cm plant spacing) and the highest LT 50 was attained at the highest stand density($494plants\;m^{-2}$ having 4.5 cm plant spacing). There were no differences in shoot morphology, winter survival, and cold hardiness in between 'Algonquin'and 'Vernal'. From this study, high stand density of alfalfa ($278plants\;m^{-2}$ or more) appears to be of little advantage compared to low stand density in terms of winter survival and cold hardiness.

• Journal of Plant Biology
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• v.32 no.4
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• pp.313-322
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• 1989

A system was established for induction of multi-shoots and plant regeneration from mesophyll protoplasts of alfalfa, Medicago sativa L. cv. Vernal. Different hormonal effects were tested at each step of protoplast culture, i.e. cell division in modified Kao's liquid medium (K566-7). calli formation on SH semi solid medium, and multi-shoot regeneration from calli on SHa and SHb solid media. Frequency of multi-shoots and plant regeneration was affected by various combinations of phytohormones in final step. The evaluation of multi-shoots induction systems via protoplast culture was discused.

• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.93-93
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• 1999

4품종의 알팔파 (Medicago sativa L.) 종자로부터 직접 캘러스를 유도하고, 형성된 캘러스로부터 식물체를 재분화하는 조건을 확립하였다. 공시품종중 "Vernal" 품종이 캘러스 유도 및 재분화에서 가장 우수한 것으로 판명되었으며, SH(Schenk and Hildebrandt), MS (Murashige and Skoog), N6 (Chu) 배지중에서 SH 배지가 캘러스 유도와 재분화시에 모두 유리한 것으로 나타났다. 캘러스 유도 및 증식시에는 2,4-D를 3mg/$\ell$ 농도로 첨가한 SH-3 배지에서 가장 효율이 좋았으며, kinetin을 첨가하거나 kinetin의 농도가 높을수록 캘러스 형성 정도는 급격히 저하되었다.(중략)저하되었다.(중략)

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.3
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• pp.190-195
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• 2020

Cadmium (Cd) toxicity is a serious limitation for agricultural production. In this study, we explored tolerance mechanism associated with Cd toxicity tolerance in alfalfa plants. We used three distinct alfalfa cultivars M. sativa cv. Vernal, M. sativa cv. Zhung Mu, and M. sativa cv. Xing Jiang Daye in this study. Cd showed declined chlorophyll score in Xing Jiang Daye compared with Zhung Mu and Vernal. No significant change observed among the cultivars for root and shoot length. Atomic absorption spectroscopy analysis demonstrated a significant accumulation of Cd, Fe, S and PC in distinct alfalfa cultivars. However, Zhung Mu and Xing Jiang Daye declined Cd accumulation in root, where Fe, S and PC incremented only in Zhung Mu. It suggests that excess Cd in Zhung Mu possibly inhibited in root by the increased accumulation of Fe, S and PC. This was further confirmed by the response of Fe (MsIRT1) and S transporters (MsSULTR1;2 and MsSULTR1;3), and MsPCS1 genes associated with Fe, S and PC availability and translocation in roots and shoots. It suggests that specially the transcript signal inducing the responses to adjust Cd especially in Zhung Mu. This finding provides the essential background for further molecular breeding program for forage crops.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.3
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• pp.124-128
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• 1989

This experiment was conducted to determine factors affecting callus induction of Vernal alfalfa. Growth regulators, basal medium, medium pH, explant age, and agar concentration for callus induction were investigated. The results obtained were as follows: For the callus induction, 2-5 mg/l 2,4-D alone was found to be most effective on callus induction. Cytokinins did not have positive effect on the callus induction, and even the more cytokinin added induced the less callus. Callus yield was much higher in B5 or SH medium than in any other media. The calli induced in PC and MS media were more friable than those induced in other media. The medium pH of 5.8 gave the best response of callus induction. At higher than pH 7.0, callus induction was inhibited severely. The effective seedling age for callus induction was around 9 days. In agar concentration, 0.5 % (W/V) was suitable for callus induction and it was severely depressed at above 1 %. Callus induction was not influenced by day length or illumination. Calli cultured under 1618 hour lightldark cycle became more compact and green than those cultured under the dark.

• Journal of the Korean housing association
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• v.13 no.6
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• pp.11-20
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• 2002

The purpose of this study is to investigate the performance level of family events, the events places where the events occur. The subjects of 169 households were selected in Jinju city and data were gathered through a questionnaire method. The data were analyzed by the SPSS package program. The major findings are as follows; 1) Most of the families perform the majority of events except lpchun (the first day of spring), the vernal Equinox Day, the Autumnal Equinox Day, the Vega festival Day, Chilsuk, and the Respect-for-the-Aged Day. 2) Birthday parties score to the highest of all family events. 3) In general, the Lunar New Year's Day, the Full Moon Harvest Day, the ancestor worship ceremony, the 15th of January (in lunar calendar), the feast of a hundred day-old baby among lifetime ceremonies, family members'birthdays, housewarming parties among the other general celebrations, and Parents Day among memorial days are held in their homes. 4) The socialized celebration of the following family events is increasing: the first birthday party, banquets for parents'60th birthday, Christmas, and wedding anniversaries.

• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.75.1-75
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• 1999

제초제 저항성 형질전환 목초 생산을 위하여 유전자총(Gene-gun) 및 Agrobacterium 기법을 이용하여 형질전환을 시도한 결과를 요약하면 다음과 같다. 1. 공시재료 : 알팔파 (cv. Vernal, Anker), 오차드그라스 (합성19호) 2. 캘러스의 유도 및 증식 : 알팔파 종자를 SH-3 배지 (SH기본배지, 2,4-D 3mg/L 포함) 그리고 오차드그라스 종자를 MS-5 배지 (MS기본배지, 2,4-D 5mg/L, 2g casein hydrolysate 포함)에 치상하여 캘러스를 유도하였다. 유도된 캘러스는 증식을 위하여 SH-3 및 MS-3 배지 (MS기본배지, 2,4-D 3mg/L, 2g casein hydrolysate 포함)에서 2주 간격으로 subculture 하였다.(중략)

• Microbiology and Biotechnology Letters
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• v.21 no.2
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• pp.95-100
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• 1993

Effect of dual inoculation with vesicular-arbuscular(VA) mycorrhizal fungi, Glomus mosseae, and Rhizobium meliloti R455 on growth, nodulation, and nitrogenase activity of alfalfa (Medicago sativa L. cv. Vernal) were examined in pot experiment. After 63 days growth, shoot length, number of leaf, and leaf size of alfalfa were increased as as result of dual inoculation with Glomus mosseae and Rhizobium meliloti. Total dry weight of alfalfa plant was increased 1.4 times compared to single inoculation with Rhizobium meliloti. Nodule number and mean fresh weight of nodule per plant were also increased due to the mycorrhizal infection.

• Journal of architectural history
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• v.1 no.1 s.1
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• pp.205-217
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• 1992

For all the variety of past studies on the pyramids of Old Kingdom of Egypt, they still basically remain in 'enigma and mystery' as Giedion rightly puts in. This paper deals directly with the three most obvious facts about the pyramids : their cardinal orientation, the varying slopes, and above all, their magnitude. The prominent triangles of their once polished faces were there to be seen from the Nile valley as they shined or shaded under the Sun. The northern faces, especially, went in and out of the Sun in accordence with the seasonal variation of the solar declination, The steeper northern faces which turned into shade in high summer noon could have been warning signs of oncoming inundation of Nile, and the milder slopes that suddenly began to shine in some early spring noons could have been the vernal alarms to awaken the peasants to their timely toil on the fresh land. Dates and hours of insolation on the northern faces of pyramids are graphically and numerically computed.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2004.11a
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• pp.291-294
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• 2004

There are many position fixing systems in the world from ancient times. But the principles are to compare the position to want to know with the known position already. The position finding system which is not restricted by weather condition and/or electronic apparatus has been sought. The best system is the GPS as far. But the system has the fatal faults as follows; 1. to depend on satellite's accuracy, 2. not to use underwater. This paper is to investigate theoretically position fixing and north finding by using free gyroscope. This paper introduce a position fixing and north finding method by measuring inclination of 2 free gyroscopes. And this system does not depend on the weather condition and underwater condition. What is more, it could use on the planets, if the gravity exits.