• Mycobiology
• /
• v.38 no.2
• /
• pp.118-121
• /
• 2010

Single ascospore isolates of Cordyceps bassiana were observed for their colony pigmentation on Sabouraud Dextrose agar plus Yeast Extract (SDAY) plates and were inoculated in a brown rice medium for production of fruiting bodies. Colony pigmentation did not show any relationship with perithecial stromata formation. The isolates were also grown on opposite sides of SDAY agar plates and were observed for vegetative compatibility. Neither vegetative compatibility nor perithecial stromata could be found to be related to each other. It was concluded that fertile fruiting body production was independent of colony pigmentation and vegetative compatibility. Synnemata formation was found to be more common than perithecial stromata formation. This might be due to its highly conidiogenous anamorphic stage, i.e., Beauveria bassiana.

• Korean Journal of Medicinal Crop Science
• /
• v.21 no.2
• /
• pp.142-147
• /
• 2013

Gastrodia elata has been cultivated as an important medicinal resources to treat various human diseases. One of the major problems associated with its field production is the degeneration of seed tubers, which is mainly caused by soil-borne pathogens. This study was conducted to produce disease-free seed tubers by the development of in vitro micropropagation method. First, tubers of G. elata were treated with $HgCl_2$ prior to culturing in vitro. Among various culture medium tested, water agar (WA) and WPM medium were the most effective on the induction and growth of vegetative stems. NAA ($0.1mg/{\ell}$) or TDZ ($1.0mg/{\ell}$) in WA medium showed better growth of vegetative stems compared to other plant hormones. Finally the induction and growth of vegetative stems were better in the dark compared to the light condition. In this study, we established an in vitro micropropagation system of G. elata, which might be an efficient way to increase the yield and quality of G. elata tubers in the field production.

• Journal of Korea Foresty Energy
• /
• v.18 no.1
• /
• pp.11-16
• /
• 1999

Dry weights of leaves, stem and floral organs of 15-year-old Tilia amurensis RUPR., were measured twice on 1 June and 20 August 1995 to examine the difference in biomass production between the reproductive and vegetative twigs which are morphologically neighboring and alternative. The following results were obtained : (1) The biomass of the reproductive twigs was greater than that of the vegetative twigs in both June and August. (2) The ratio of stem to total biomass in the reproductive and the vegetative twigs was greater in August than that in June, while the ratio of leaf biomass was greater in June than that in August. The ratio of floral organ to the total biomass in the reproductive twigs was 14.6% and 27.1% in June and August, respectively. (3) The total twig biomass per leaf biomass was greater in the reproductive twigs than that in the vegetative twigs in both June and August. (4) Net assimilation rate in the floral organs showed 21% of that in the leaves in June and 37% in August.

• Horticultural Science & Technology
• /
• v.35 no.1
• /
• pp.21-29
• /
• 2017

This study was conducted to determine the effects of artificial lighting sources on vegetative growth of Phalaenopsis and Doritaenopsis (an intergeneric hybrid of Doritis and Phalaenopsis) orchids. One - month - old plants were cultivated under fluorescent lamps, cool - white light - emitting diodes (LEDs), or warm - white LEDs at 80 and $160{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The blue (400 - 500 nm) : green (500 - 600 nm) : red (600 - 700 nm) : far - red (700 - 800 nm) ratios of the fluorescent lamps, cool-white LEDs, and warm-white LEDs were 1 : 1.3 : 0.8 : 0.1, 1 : 1.3 : 0.6 : 0.1, and 1 : 2.7 : 2.3 : 0.4, respectively. Each light treatment was maintained for 16 weeks in a closed plant-production system maintained at $28^{\circ}C$ with a 12 h photoperiod. The longest leaf span, as well as the leaf length and width of the uppermost mature leaf, were observed in plants treated with warm-white LEDs. Plants grown under fluorescent lamps had longer and wider leaves with a greater leaf span than plants grown under cool-white LEDs, while the maximum quantum efficiency of photosystem II was higher under cool-white LEDs. The vegetative responses affected by different lighting sources were similar at both 80 and $160{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Leaf span and root biomass were increased by the higher light intensity in both cultivars, while the relative chlorophyll content was decreased. These results indicate that relatively high intensity light can promote vegetative growth of young Phalaenopsis plants, and that warm - white LEDs, which contain a high red-light ratio, are a better lighting source for the growth of these plants than the cool-white LEDs or fluorescent lamps. These results could therefore be useful in the selection of artificial lighting to maximize vegetative growth of Phalaenopsis plants in a closed plant - production system.

• The Korean Journal of Ecology
• /
• v.20 no.5
• /
• pp.367-373
• /
• 1997

The effects of salinity on the growth and production of rice were studied at Seosan reclaimed land from July to October, 1995. The plant height, the number of living aleaves, dead leaves and total leaves, the number of the grains and the dry weight of the grains per individual, and the dry weight of above the ground in $25cm{\times}25cm$ quadrat were investigated on 5 plots whers were different salinity. The plant height, the number of living leaves and total leaves and the number of grains and the dry weight of grains per individual, and the dry weight of above the ground decreased as the salinity of water increase and the number of dead leaves of rice increased as the salinity of water decrease. The effect of salinity on the reproductive production is severer than the vegetative production. Because of the salinity, the growth and the production of the rice at Seosan reclaimed land are worse than the normal rice field.

• Journal of Fisheries and Marine Sciences Education
• /
• v.23 no.1
• /
• pp.126-129
• /
• 2011

Vegetative morphological information of the red alga Neorhodomela larix (Turner) Masuda (Rhodomelaceae) is given. This species is characterized by thalli up to 15 cm high, brownish black in color, pericentral cells with transverse division, the first order subulate branches with determinate growth, numerous adventitious branches produced at axils and absence of vegetative trichoblasts. Particularly, N. larix is distinct from other Korean Neorhodomela species by the lack of vegetative trichoblasts, which seems to be caused by their delayed production until reproductive structures are formed. This is the first record of N. larix in Korea.

• The Plant Pathology Journal
• /
• v.17 no.5
• /
• pp.276-280
• /
• 2001

Fusarium isolates of Gibberella fujikuroi species complex were obtained from sorghum grown in five provinces of Korea in 1996 and 1997. These isolates were characterized based on their mating behavior, mycotoxin production, and vegetative compatibility. Only three mating populations (A, D, and F) were recovered from a total of 155 isolates examined. The relative frequency of the mating populations was significantly different: F was predominant (80%), while D and A were observed at low frequencies of 9% and 3%, respectively. Female fertile isolates were more common within F (44 our of 124) than D (2 out of 14), while none of the five A isolates were female fertile. The inbreeding effective population sizes ($\textrm{N}_e$)for mating type and male/hermaphrodite ratios in mating populations A and D produced significant amounts of fumonisins, while F isolates produced none or only traces of fumonisin B$_1$. In contrast. F isolates produced higher amounts of moniliformin (average of 3,820 ppm) than A and D isolates (averages of 77 and 1,819 ppm, respectively). Fifty-one isolates were tested for vegetative compatibility using nitrogen non-utilization mutants of each isolate, and 44 vegetative compatibility groups (VCGs) were identified. A single VC type (VC1) was found in all of the five A isolates examined. Six of the D isolates examined consisted of three VC types: two for VC2, two for VC3, and the rest for VC4. All of the F isolates tested were incompatible in every combination and , thus, each constituted a unique VCG.

• ALGAE
• /
• v.30 no.2
• /
• pp.121-137
• /
• 2015

Studies on carbon flux in the oceans have been highlighted in recent years due to increasing awareness about climate change, but the coastal ecosystem remains one of the unexplored fields in this regard. In this study, the dynamics of carbon flux in a vegetative coastal ecosystem were examined by an evaluation of net and gross ecosystem production (NEP and GEP) and $CO_2$ exchange rates (net ecosystem exchange, NEE). To estimate NEP and GEP, community production and respiration were measured along different habitat types (eelgrass and macroalgal beds, shallow and deep sedimentary, and deep rocky shore) at Gwangyang Bay, Korea from 20 June to 20 July 2007. Vegetative areas showed significantly higher ecosystem production than the other habitat types. Specifically, eelgrass beds had the highest daily GEP ($6.97{\pm}0.02g\;C\;m^{-2}\;d^{-1}$), with a large amount of biomass and high productivity of eelgrass, whereas the outer macroalgal vegetation had the lowest GEP ($0.97{\pm}0.04g\;C\;m^{-2}\;d^{-1}$). In addition, macroalgal vegetation showed the highest daily NEP ($3.31{\pm}0.45g\;C\;m^{-2}\;d^{-1}$) due to its highest P : R ratio (2.33). Furthermore, the eelgrass beds acted as a $CO_2$ sink through the air-seawater interface according to NEE data, with a carbon sink rate of $0.63mg\;C\;m^{-2}\;d^{-1}$. Overall, ecosystem production was found to be extremely high in the vegetated systems (eelgrass and macroalgal beds), which occupy a relatively small area compared to the unvegetated systems according to our conceptual diagram of a carbon-flux box model. These results indicate that the vegetative ecosystems showed significantly high capturing efficiency of inorganic carbon through coastal primary production.

• The Korean Journal of Food And Nutrition
• /
• v.15 no.4
• /
• pp.364-369
• /
• 2002

Streptomyces shows a eukaryotic characteristic that vegetative cell can grow into mycelial form and has morphological and physiological differentiation at a certain period during its life cycle. Streptomyces has been used for the production of many biologically active compounds, such as antibiotics and pronase. Production of second metabolites and differentiation of the vegetative cell share the certain period of its lift cycle. Therefore, second metabolites may affect the differentiation of the vegetative cell. One of the microbial hormone, called A-factor, regulates the production of second metabolites, sporulation and differentiation of the cells. Streptomyces griseus produces streptomycin as well as many different kinds of proteinase. As mentioned, period of proteinases production overlaps with the period of differentiation of the vegetative cells. Protease may play a important role for the differentiation of the cells. In this paper, function of the SGPD gene cloned from S. griseus IFO 13350 tested whether it affects for the differentiation of A-factor mutated S. griseus HH1 and S. griseus IFO13350. pWHM3 and pWHM3-sprD plasmid was transformed into S. griseus HH1 and S. griseus IFO13350. Chymotrypsin activity of the cultured medium of the transformants with pWHM3-sprD plasmid didn't show any change with that of the transformants with plasmid only. The transformants with pWHM3-sprD plasmid didn't show the increase of the production of actinorhodin as well as morphological change in S. griseus IFO 13350 and HH1, as well. The promoter sequences of the SGPA and SGPB gene which encode chymotrypsin-like protease, were compared with that of SGPD gene. Regulatory mechanism of gene expression of proteinase genes will be studied for the development of high production system for protease as well as the function of the proteases.

• Journal of Forest and Environmental Science
• /
• v.32 no.2
• /
• pp.113-119
• /
• 2016

The long lived iteroparous conifers produce male and female gametophyte for hundreds of years once they reach the reproductive stage, however, the production of seed is not frequent. This phenomenon of infrequent seed production in conifers is yet to be understood. An attempt to study this change in cone production in Cedrus deodara of Western Himalayas -a species reported to be mainly monoecious and rarely dioecious has been made. The observations recorded on selected trees of flowering/fruiting stage for four years at four different locations have shown the species to be dioecious with higher percentage of female trees during good seed year but with no definite or predictable pattern of reproduction in the trees. A decline in production of female trees was witnessed at all but one location immediately year after reaching the maximum (good seed year). The phenomenon of seed production has been observed to be cyclic and site specific. A change in the sexual behavior of the trees from male to female or vice-versa, male or female turning monoecious or vegetative and vegetative coming to reproductive was unpredictable.