• 제목/요약/키워드: Vegetative Stage

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들깨의 생육단계와 부위별 기능성화합물 함량 비교 분석 (Comparative Analysis of Functional Compounds in Perilla frutescens at Different Stages and Growth Times)

  • 김해은;윤희랑;허재복
    • 생명과학회지
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    • 제31권5호
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    • pp.511-519
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    • 2021
  • 들깨(Perilla frutescens var. japonica HARA)는 대표적인 유지작물 중 하나로서, 목적에 따라 잎들깨와 종실들깨로 구분된다. 들깨는 식용과 약용으로 사용되어 왔으며, 들깨의 잎, 줄기, 종자에는 다양한 기능성 성분이 많이 함유되어 있다고 알려져 있다. 본 연구는 들깨를 기능성 소재로서 이용하고자 최적의 수확시기 및 조건을 분석하기 위해 새싹들깨와 잎들깨의 생육단계별 및 부위별로 들깨의 주요 기능성 화합물인 rosmarinic acid, caffeic acid, luteolin, GABA의 함량 비교하였다. 새싹들깨의 기능성 화합물 분석 결과 자연광 조건하의 흙에서 재배한 새싹들깨가 rosmarinic acid 23.19±0.16 mg/g과 GABA 0.55±0.05 mg/g으로 높은 함량을 보였고, 파종 후 6~8일 시기에 rosmarinic acid, caffeic acid, GABA의 함량이 가장 높게 나타났다. 따라서 자연광 조건하의 흙에서 파종 후 6~8일이 새싹들깨의 최적의 수확조건으로 판단된다. 생육단계별 및 부위별로 잎들깨의 기능성 화합물 변화 분석결과 caffeic acid와 rosmarinic acid는 영양생장에서 생식생장으로 넘어가는 단계에 0.28±0.03~0.30±0.07 mg/g과 20.60±7.02~19.37±3.18 mg/g으로 함량이 가장 높았으며, luteolin은 생식생장단계에 31.11±2.98~22.35±1.64 ㎍/g으로 함량이 가장 높았다. GABA는 영양생장 초기단계에서 0.42±0.09~0.37±0.04 mg/g으로 가장 높은 함량을 보였다. 부위별 분석 결과 잎, 줄기, 추대 부위 중 추대부위가 rosmarinic acid (55.22±9.33 mg/g), caffeic acid (0.39±0.03 mg/g), luteolin (1044.89±6.72 ㎍/g)으로 함량이 가장 높게 나타났다. 종합적으로 새싹들깨와 잎들깨에서 기능성 화합물이 가장 증가하는 시기를 확인함으로써 적합한 수확시기 및 부위를 확인하였다. 본 연구는 기능성 소재로서 들깨를 이용하기 위해 새싹들깨와 잎들깨의 적합한 수확시기 및 조건을 제시하였다.

일(日)·인원연교잡(印遠緣交雜) 수도품종(水稻品種)의 급성위조증상(急性萎凋症狀) 발생(發生)에 관(關)한 영양생리학적(營養生理學的) 연구(硏究) -III. 수도근계(水稻根系)의 발달(發達)과 위조증상(萎凋症狀) 발생(發生)과의 관계(關係) (Physiological studies on the sudden wilting of JAPONICA/INDICA crossed rice varieties in Korea -III. Relationship between wilting injury and the rice root developing system)

  • 김유섭
    • 한국토양비료학회지
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    • 제22권2호
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    • pp.122-126
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    • 1989
  • 위조증상발생(萎凋症狀發生)에서 볼수 있는 품종간차이(品種間差異)를 근계(根系)의 발달(發達) 및 활력(活力)의 특징(特徵)에서 구명(究明)하기 위(爲)하여 농가포장(農家圃場) 및 시험포장(試驗圃場)에서 실태조사(實態調査), 사경재배(砂耕栽培)에 의(依)한 대조품종(對照品種)의 생육시기별조사등(生育時期別調査等)을 실시(實施)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 급성위조증상(急性萎凋症狀)이 발생(發生)한 "유신(維新)"은 건전주(健全株)에 비(比)해 피해주(被害株)에서 T/R비율(比率)이 높았다. 2. 급성위조증상(急性萎凋症狀)이 발생(發生)한 "유신(維新)"은 건전주(健全株)에 비(比)해 부위(部位)에 관계(關係)없이 근활력(根活力)이 저하(低下)하였다 "유신(維新)"의 영양생장기(營養生長期)에 있어서의 지상부생육량(地上部生育量)은 타(他) 품종(品種)보다 크나 T/R률(率)은 높아지는 경향(傾向)이며, 생육후기(生育後期)에 있어서의 광합성산물(光合成産物)의 근(根)에의 배분량(配分量)이 상대적(相對的)으로 감소(減少)하는것으로 보아 생식생장기(生殖生長期)에 있어서 근(根)의 활력저하(活力低下)를 초래(招來)하는 요인(要因)이 되는 것으로 추정(推定)된다. 3. 위조증상(萎凋症狀)이 발생(發生)한 "유신(維新)"은 영양생장기(營養生長期)의 근활력(根活力)이 높고, 지상부(地上部)의 생장(生長)도 왕성(旺盛)하나 생식생장기(生殖生長期)에는 타(他) 품종(品種)에 비(比)해 활력(活力)이 현저(顯著)하게 저하(低下)하는 특징(特徵)을 나타내는 것이 명확(明確)하게 밝혀졌다. 4. 급성(急性) 위조증상발생(萎凋症狀發生)이 비교적(比較的) 적었던 "밀양(密陽) 23호(號)"의 근활력(根活力)은 피해주(被害株)와 건전주간(健全株間)에 차이(差異)가 없었으며, 양자(兩者) 공(共)히 후기(後期)까지 비교적(比較的) 높게 유대(維待)되었으며, 근(根)도 굵고 T/R비율(比率)도 낮았다.

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참깨의 단경, 분지형에 있어서 인산함량이 함유율과 지상부 생육에 미치는 영향 (The Effects of Phosphate to the Growth and Oil Contents in Sesame(Sesamum indicum L.))

  • 서관석;김준기;김소연;이주열;최창열
    • 한국작물학회지
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    • 제29권4호
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    • pp.314-320
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    • 1984
  • 참깨의 현장려 품종중 단경형과 분지형 두 품종에 대한 인산의 시비수준에 따른 생육시기별 지상부 생육 및 함유량을 조사한 결과 가. 광합성량은 초기(파종후 6주까지)에는 풍년깨가 후기에는 광산깨가 높았으며, 두 품종 모두 인산10a당4 시용구에서 광합성이 가강 높았다. 나. 인산의 시비수준에 따라 식물체의 무기성분 함량간에는 큰 차이는 없었으나, 시기별 인산함량은 파종후 3주가, 질소와 가리는 파종후 6주가 가장 높았고, 엽분석결과 인산함량과 지방과는 0.48*, 가리함량과 탄수화물과는 0.57**로서 모두 유의성이 인정되었다. 다. 개화최성기(7월 15일)의 광합성능력과 상대생장율(RGR) 및 순동화율(NAR)간에는 품종별로는분지형인 광산깨가 인산시용량간에는 10a당 4kg시용구에서 가장 높았다. 라. 참깨의 품종별 종실중은 풍년깨는 10a당 4kg시용구가, 광산깨는 10a당8kg시용구가 가장 많았으나, 동일종실중에 대한 함유량은 두 품종 모두 인산의 시비량이 많을수록 증대되었다.

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Direct Evidence of Endophyte (Neotyphodium coenophialum) Genotype Effect on Growth and Vertical Transmission of Endophyte in Tall Fescue (Schedonorus phoenix Scop.) Under Water Stress

  • Ju, Ho-Jong
    • The Plant Pathology Journal
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    • 제27권3호
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    • pp.249-256
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    • 2011
  • Tall fescue (Schedonorus phoenix Scop.) is resistant to abiotic and biotic stresses through a symbiotic relationship with Neotyphodium coenophialum. However, this endophyte has been considered detrimental since it produces toxic alkaloids to animals. It is vital to understand mutuality between these two to maximize positive impact of the endophyte on agri-ecosystem. Little research has been conducted on endophyte transmission mechanism in planta. To provide basic information related to endophyte transmission, an experiment was conducted to examine the effect of endophyte genotype and water stress on endophyte transmission by imposing soil moisture deficits at different stages of panicle development. There was water stress effect on endophyte frequency but not on concentration, whereas endophyte genotype significantly influenced endophyte concentration in pseudostem of tall fescue at boot stage. Reproductive tillers showed greater endophyte frequency and concentration. Endophyte frequency in florets or seeds depended on position within panicle. There was no drought effect on endophyte concentration, but showed the effect of endophyte genotype on endophyte concentration in florets and seeds. Overall endophyte concentration in seeds was higher. From this study, we may conclude that although water stress reduced endophyte frequency in vegetative tiller, water stress does not have effect on endophyte transmission, suggesting that drought is not an important factor controlling the endophyte transmission from plant to seed. Endophyte genotype and seed position in a panicle affected endophyte transmission, indicating that these two factors are involved in endophyte transmission and may determine seed transmission of endophyte in tall fescue.

Morphology and Reproduction of Acrosorium polyneurum and A. yendoi (Delesseriaceae, Rhodophyta) in Korea

  • Kim Young Sik;Choi Han Gil;Nam Ki Wan;Hong Yong Ki;Lee Jong Hwa
    • Fisheries and Aquatic Sciences
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    • 제1권1호
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    • pp.1-6
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    • 1998
  • Morphology and reproduction of the two similar Acrosorium species, A. polyneurum and A. yendoi, were studied based on specimens collected from Korea. The morphology of the former species was very variable, depending on its habitat, and in some cases shown superficial resemblance to that of A. yendoi. Also its reproductive structures were essentially the same as those of the latter. However, the two species appear to be distinguished by some vegetative features found in fully developed stage, such as thallus size, vein structures and branching pattern. Acrosorium polyneurum has comparatively large thallus (6-8cm) with three to five cell-layered macroscopic veins, together with palmately dichotomously branching, whereas A. yendoi is of smaller thallus (3-6cm) with microscopic veins of one to three cell layers, and shows irregularly dichotomously or pinnately branching. This result, together with recent data based on PCR technique, suggests that the two entities are distinct.

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Response of Leaf Water Potential and Growth Characteristics to Irrigation Treatment in Soybean

  • Lee, Jeong-Hwa;Seong, Rak-Chun
    • 한국작물학회지
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    • 제48권2호
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    • pp.81-88
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    • 2003
  • Soybeans [Glycine max (L.) Merr.] are frequently exposed to unfavorable environments during growing seasons and water is the most important factor limiting for the production system. The purpose of this study was to determine the leaf water potential changes by irrigation, and to evaluate the relationships of leaf water potential, growth and yield in soybeans. Three soybean cultivars, Hwangkeumkong, Shinpaldalkong 2, and Pungsannamulkong, were planted in growth chamber and field with irrigated treatments. Leaf water potential of three soybean cultivars was positively correlated with leaf water content during vegetative and reproductive growth stages in growth chamber and field experiments. Leaf water potentials measured for three soybean cultivars under growth chamber were higher than those of under field conditions. Higher leaf water potential with irrigated plots under field was observed compared to conventional plots during reproductive growth stages. Leaf water potentials of three soybean cultivars were continually decreased during reproductive growth stages under field and there was no significant difference among them. Number of leaves, leaf water content, pod dry weight, number of seeds and seed dry weight with irrigated plots were higher than those of conventional plots. The results of this study suggested that leaf water potential could be used as an important growth indicator during the growing season of soybean plants.

Estimating Optimal-Band of NDVI and GNDVI by Vegetation Reflectance Characteristics of Crops.

  • Shin, Hyoung-Sub;Park, Jong-Hwa;Park, Jin-Ki;Kim, Seong-Joon;Lee, Mi-Seon
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2008년도 International Symposium on Remote Sensing
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    • pp.151-154
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    • 2008
  • Information on the area and spatial distribution of crop fields is needed for biomass production, arrangement of water resources, trace gas emission estimates, and food security. The present study aims to monitor crops status during the growing season by estimating its aboveground biomass and leaf area index (LAI) from field reflectance taken with a hand-held radiometer. Field reflectance values were collected over specific spectral bandwidths using a handheld radiometer(LI-1800). A methodology is described to use spectral reflectance as indicators of the vegetative status in crop cultures. Two vegetation indices were derived from these spectral measurements. In this paper, first we analyze each spectral reflectance characteristics of vegetation in the order of growth stage. Vegetation indices (NDVI, GNDVI) were calculated from crop reflectance. And assess the nature of relationships between LAI and VI, as measured by the in situ NDVI and GNDVI. Among the two VI, NDVI showed predictive ability across a wider range of LAI than did GNDVI. Specific objectives were to determine the relative accuracy of these two vegetation indices for predicting LAI. The results of this study indicated that the NDVI and GNDVI could potentially be applied to monitor crop agriculture on a timely and frequent basis.

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Dry Matter Accumulation and Leaf Mineral Contents as Affected by Excessive Soil Water in Soybean

  • Seong, Rak-Chun;Kim, Jeong-Gyu;Nelson, C. Jeny
    • 한국작물학회지
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    • 제44권2호
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    • pp.129-133
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    • 1999
  • Excessive soil water at vegetative growth stages during the rainy season induces yield losses in soybeans. Our objectives were to obtain basic information about the cultivar differences and to understand the stress-tolerance process for due to excessive soil water. Previous experiments revealed soybean genotypic differences in tolerance to excessive soil water. A field experiment was conducted at the Research Farm of Korea University near Seoul on 21 May 1998. Soybean[Glycine max (L.) Merrill] cultivars, 'Hannamkong' (sensitive) and 'Taekwan-gkong'(tolerant) were planted in vinyl-lined plots(1.2 x 4.2 x 0.3 m deep) and control plots. Drip irrigation began at VI growth stage to submerge the soil surface. Three weeks of excessive soil water treatment reduced all growth parameters measured to soybean plants. Excessive soil water stress resulted in decreases of N, P, K, Ca, Mg and Cu, and increases of Fe and Mn contents in soybean leaves. The stress index of tolerant cultivars under excessive soil water showed no large difference in soybean growth characteristics measured at three growth stages. However, K, Ca, Mg, Fe and Mn contents in soybean leaves appeared to differ between sensitive and tolerant cultivars. From the above results, stress and tolerance indices are proposed for a method to test cultivar differences in plant responses within a species under adverse growth environments.

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Cellular Features of the Fronds and Turions in Spirodela polyrhiza

  • Kim, InSun
    • Applied Microscopy
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    • 제43권4호
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    • pp.140-145
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    • 2013
  • Structural aspects of highly reduced vegetative organs in the aquatic Spirodela polyrhiza were examined using scanning and transmission electron microscopy. The study focused mainly on young and mature fronds with turions and their cellular features were compared. Mature fronds were composed of thin-walled chlorenchyma with highly vacuolated cells; most of which were frequently occupied by either tanniferous deposits or various crystals. Fronds of photoautotrophic offspring were produced from the meristematic region of the reproductive pockets within mother fronds, where they remained until separation. Moderate degrees of wall ingrowth and plasmalemma proliferation were detected briefly in the epidermis of daughter fronds during early development. Vascular tissues were generally much reduced, but air chambers were well-established in fronds. Chloroplasts having grana with several thylakoids were distributed throughout the plant, but starch grains were encountered frequently in the mesophyll chloroplasts of younger fronds and initial stage of the turion. Their cytoplasm was dense with small vacuoles in most cases. Further, big starch grains, up to several microns, occupying most of the plastid volume were formed in the turion prior to sink for overwintering. Plasmodesmata were numerous in the examined tissues, except mature turions, suggesting a symplastic pathway of the metabolites within body.

Effects of Microbe-inoculated Expanded Rice Hull on Growth, Yield and Grain Quality of Rice

  • Kim, Young Jun;Nunez, John Paolo;Seo, Pil Dae;Ultra, Venecio U. Jr.;Lee, Sang Chul
    • 한국작물학회지
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    • 제58권1호
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    • pp.78-83
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    • 2013
  • The excessive and indiscriminate use of chemical fertilizers in the past has brought serious soil and other environmental problems so alternatives over this agrochemical are being searched. Our study focuses on the effects of expanded rice hull inoculated with selected beneficial microorganisms on growth (through agronomic characters), yield and yield components, and grain quality indices of rice. Results showed that favorable effects of different expanded rice hull preparations were not readily apparent at vegetative stage and only treatments with supplemental chemical fertilizer application were comparable with the conventional practice. Expanded rice hull combined with 50% rate of chemical fertilizer exhibited a significantly higher yield (6,471 kg $ha^{-1}$) over conventional practice (5,719 kg $ha^{-1}$). Good milling quality indices were observed in treatments having 50% chemical fertilizers plus alternatives from expanded rice hull. Finally, we demonstrated that chemical fertilizer rate can potentially be reduced into 50% if combined with expanded rice hull, and show even better output than chemical fertilizer alone.