• Journal of Korean Neurosurgical Society
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• v.64 no.5
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• pp.705-715
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• 2021

Objective : Through our previous clinical trials, the demonstrated therapeutic effects of MSC in chronic spinal cord injury (SCI) were found to be not sufficient. Therefore, the need to develop stem cell agent with enhanced efficacy is increased. We transplanted enhanced Wnt3-asecreting human mesenchymal stem cells (hMSC) into injured spines at 6 weeks after SCI to improve axonal regeneration in a rat model of chronic SCI. We hypothesized that enhanced Wnt3a protein expression could augment neuro-regeneration after SCI. Methods : Thirty-six Sprague-Dawley rats were injured using an Infinite Horizon (IH) impactor at the T9-10 vertebrae and separated into five groups : 1) phosphate-buffered saline injection (injury only group, n=7); 2) hMSC transplantation (MSC, n=7); 3) hMSC transfected with pLenti vector (without Wnt3a gene) transplantation (pLenti-MSC, n=7); 4) hMSC transfected with Wnt3a gene transplantation (Wnt3a-MSC, n=7); and 5) hMSC transfected with enhanced Wnt3a gene (1.7 fold Wnt3a mRNA expression) transplantation (1.7 Wnt3a-MSC, n=8). Six weeks after SCI, each 5×10⁵ cells/15 µL at 2 points were injected using stereotactic and microsyringe pump. To evaluate functional recovery from SCI, rats underwent Basso-Beattie-Bresnahan (BBB) locomotor test on the first, second, and third days post-injury and then weekly for 14 weeks. Axonal regeneration was assessed using growth-associated protein 43 (GAP43), microtubule-associated protein 2 (MAP2), and neurofilament (NF) immunostaining. Results : Fourteen weeks after injury (8 weeks after transplantation), BBB score of the 1.7 Wnt3a-MSC group (15.0±0.28) was significantly higher than that of the injury only (10.0±0.48), MSC (12.57±0.48), pLenti-MSC (12.42±0.48), and Wnt3a-MSC (13.71±0.61) groups (p<0.05). Immunostaining revealed increased expression of axonal regeneration markers GAP43, MAP2, and NF in the Wnt3a-MSC and 1.7 Wnt3a-MSC groups. Conclusion : Our results showed that enhanced gene expression of Wnt3a in hMSC can potentiate axonal regeneration and improve functional recovery in a rat model of chronic SCI.

• Journal of Intelligence and Information Systems
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• v.28 no.3
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• pp.119-137
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• 2022

Recent recommendation system studies apply various deep learning models to represent user and item interactions better. One of the noteworthy studies is ONCF(Outer product-based Neural Collaborative Filtering) which builds a two-dimensional interaction map via outer product and employs CNN (Convolutional Neural Networks) to learn high-order correlations from the map. However, ONCF has limitations in recommendation performance due to the problems with CNN and the absence of side information. ONCF using CNN has an inductive bias problem that causes poor performances for data with a distribution that does not appear in the training data. This paper proposes to employ a Vision Transformer (ViT) instead of the vanilla CNN used in ONCF. The reason is that ViT showed better results than state-of-the-art CNN in many image classification cases. In addition, we propose a new architecture to reflect side information that ONCF did not consider. Unlike previous studies that reflect side information in a neural network using simple input combination methods, this study uses an independent auxiliary classifier to reflect side information more effectively in the recommender system. ONCF used a single latent vector for user and item, but in this study, a channel is constructed using multiple vectors to enable the model to learn more diverse expressions and to obtain an ensemble effect. The experiments showed our deep learning model improved performance in recommendation compared to ONCF.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.11
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• pp.1636-1650
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• 2007

As an initial step toward a better understanding of the genome structure of Korean cattle (Hanwoo breed) and initiation of the framework for genomic research in this bovine, the bacterial artificial chromosome (BAC) end sequencing of 21,024 clones was recently completed. Among these clones, BAC End Sequences (BESs) of 20,158 clones with high quality sequences (Phred score ${\geq}20$, average BES equaled 620 bp and totaled 23,585,814 bp), after editing sequencing results by eliminating vector sequences, were used initially to compare sequence homology with the known bovine chromosomal DNA sequence by using BLASTN analysis. Blast analysis of the BESs against the NCBI Genome database for Bos taurus (Build 2.1) indicated that the BESs from 13,201 clones matched bovine contig sequences with significant blast hits (E<$e^{-40}$), including 7,075 single-end hits and 6,126 paired-end hits. Finally, a total of 5,105 clones of the Korean cattle BAC clones with paired-end hits, including 4,053 clones from the primary analysis and 1,052 clones from the secondary analysis, were mapped to the bovine chromosome with very high accuracy.

• Journal of Korea Society of Industrial Information Systems
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• v.12 no.2
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• pp.49-59
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• 2007

This paper is about an system which can perform automatic semantic annotation to actualize "Semantic Web." Since it is impossible to tag numerous documents manually in the web, it is necessary to gather large Korean web documents as training data, and extract features by using natural language techniques and a thesaurus. After doing these, we constructed concept classifiers through the SVM (support vector machine) teaming algorithm. According to the characteristics of Korean language, morphological analysis and syntax analysis were used in this system to extract feature information. Based on these analyses, the concept code is mapped with Kadokawa thesaurus, which made it possible to map similar words and phrase to one concept code, to make training vectors. This contributed to rise the recall of our system. Results of the experiment show the system has a some possibility of semantic annotation.

• Journal of Broadcast Engineering
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• v.9 no.3
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• pp.196-206
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• 2004

This paper presents an efficient joint disparity-motion estimation algorithm for stereo sequence CODEC. Disparity vectors are estimated by the left and right motion vectors and previous disparity vectors for every frame. In order to obtain more accurate disparity vectors. we include a spatial prediction Process after the feint estimation. From joint estimation and spatial prediction, we can obtain accurate disparity vectors and then Increase coding efficiency. Finally, we proposed the backward quadtree decomposition. which helps the encoder to have a more detailed disparity vector map without transmitting additional coding bits for quadtree information. We confirmed superior performance of the proposed method through computer simulation.

• The Journal of Engineering Geology
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• v.26 no.1
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• pp.23-32
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• 2016

This study evaluates landslide susceptibility using statistical analysis by SVM (support vector machine) and the illite index of clay minerals extracted from ASTER(advanced spaceborne thermal emission and reflection radiometer) imagery which can be use to create mineralogical mapping. Landslide locations in the study area were identified from aerial photographs and field surveys. A GIS spatial database was compiled containing topographic maps (slope, aspect, curvature, distance to stream, and distance to road), maps of soil properties (thickness, material, topography, and drainage), maps of timber properties (diameter, age, and density), and an ASTER satellite imagery (illite index). The landslide susceptibility map was constructed through factor correlation using SVM to analyze the spatial database. Comparison of area under the curve values showed that using the illite index model provided landslide susceptibility maps that were 76.46% accurate, which compared favorably with 74.09% accuracy achieved without them.

• Journal of Korea Spatial Information System Society
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• v.5 no.1 s.9
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• pp.83-89
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• 2003

The GIS which is analysis of the real world to construction of 3D virtual city develop s in rapid growth. 3D virtual city is simulated by use of a GIS, Computer graphics, virtual reality and Spatial database. In this paper 3D topographical basemap which have the geographic coordinate of world was constructed by digital map. And build the Cyber city with combining the building that have elevation and 3D topographical basemap. By using server/client system for the cyber city. 3D cyber city web service implemented. We proposed the hybrid base web service method rather than the image or vector base web service. By the spatial and attribute search in the 3D Cyber City, overcome the existing simple visibility of Web 3D technology, and exhibited development direction to the Web 3D GIS.

• Korean Journal of Microbiology
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• v.23 no.2
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• pp.138-146
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• 1985

A Bacillus licheniformis ATCC31667 gene coding for a glucose isomerase has been cloned and expressed in glucose isomerase negative mutant of Escherichia coli. A recombinant plasmid, constructed by ligation of a EcoRI fragment of B.licheniformis chromosomal DNA to vector plasmid pBR322, was expressed glucose isomerase positive in E.coli LE392-6 with growth on minimal medium containing xylose as a sole carbon source. This recombinant plasmid, designated pBGI6, had the insery of 4.1Kb of Bacillus gene in EcoRI site, and restriction map of the plasmid was established. The plasmid pBG16 was very stable after 10days of serial transfer to a fresh medium. The activity of glucose isomerase from the transformed cell containing pBGI6 was increased about 20 fold than its wild type of host.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.10B
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• pp.1986-1994
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• 1999

In this paper, a semi-automatic approach is adopted to extract a semantic object from real-world video sequences human-aided segmentation for the first frame and automatic tracking for the remaining frames. The proposed algorithm has a hierarchical structure using watershed algorithm. Each hierarchy consists of 3 basic steps: First, seeds are extracted from the simplified current frame. Second, region growing bv a modified watershed algorithm is performed to get over-segmented regions. Finally, the segmented regions are classified into 3 categories, i.e., inside, outside or uncertain regions according to region probability values, which are acquired by the probability map calculated from an estimated motion-vector field. Then, for the remaining uncertain regions, the above 3 steps are repeated at lower hierarchies with less simplified frames until every region is classified into a certain region. The proposed algorithm provides prospective results in studio-quality sequences such as 'Claire', 'Miss America', 'Akiyo', and 'Mother and daughter'.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.6B
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• pp.1183-1190
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• 1999

This paper presents an efficient hardware architecture to improve the frame memory interface occupying the largest hardware area together with motion estimator in implementing MPEG-2 video encoder as an ASIC chip. In this architecture, the memory size for internal data buffering and hardware area for frame memory interface control logic are reduced through the efficient memory map organization of the external SDRAM having dual bank and memory access timing optimization between the video encoder and external SDRAM. In this design, 0.5 m, CMOS, TLM (Triple Layer Metal) standard cells are used as design libraries and VHDL simulator and logic synthesis tools are used for hardware design add verification. The hardware emulator modeled by C-language is exploited for various test vector generation and functional verification. The architecture of the improved frame memory interface occupies about 58% less hardware area than the existing architecture[2-3], and it results in the total hardware area reduction up to 24.3%. Thus, the (act that the frame memory interface influences on the whole area of the video encoder severely is presented as a result.