• Title/Summary/Keyword: Vector Reduce

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Prevalence of Salmonella Species Isolated from Old Hen Delivery Trucks in Korea and Application of Disinfectant for the Reduction of Salmonella Contamination (국내 노계 운반 전용 차량에서의 살모넬라 오염율 및 차량소독에 따른 오염도 감소 효과)

  • Na, Deok-Hwan;Hong, Young-Ho;Yoon, Mi-Young;Park, Eun-Jin;Lim, Tae-Hyun;Jang, Jun-Hyuk;Kim, Byoung-Youn;Lee, Dong-Hun;Song, Chang-Seon
    • Korean Journal of Poultry Science
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    • v.40 no.1
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    • pp.11-16
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    • 2013
  • Salmonella is closely related with human health of modern society which has concern increased in livestock goods consumption as well as give economic damage throughout the chicken industry such as farm, hatchery, slaughter house and processing plant. From 2007 to 2011, this study investigated Salmonella prevalence from 200 old hen delivery trucks which deliver old egg-laying hens and broiler breeders in Korea. The prevalence of Salmonella species was 38.0% in old hen delivery truck. Serogroup C1 was the most frequently detected serogroup of Salmonella, followed by the serogroups D1, C2 and B. A total of 25 serotypes were identified and Salmonella infantis was the most frequently isolated serotype. In addition, we applied disinfectant to old hen delivery truck for the reduction of Salmonella contamination. The disinfectant consists of formaldehyde, glutaladehyde and quaternary ammonium compound was applied to the trucks. Salmonella isolation rate was significantly decreased after disinfection from 38.0% to 7.5%. Disinfectant could not effectively reduce Salmonella contamination at a dilution of 1:200 which is recommended by manufacturer, but Salmonella isolation rate was significantly decreased at a dilution of 1:50. Since old hen delivery truck could be a potential vector to carry Salmonella into farms and abattoirs, chicken delivery truck should be disinfected thoroughly and sufficiently to control contamination of farms and abattoirs.

Investigation on Inhibitory Effect of ErmSF N-Terminal End Region Peptide on ErmSF Methyltansferase Activity In Vivo Through Development of Co-Expression System of Two Different Proteins in One Cell (서로 다른 두 단백질의 세포 내 동시 발현 체계의 개발을 통한 ErmSF에서 특이적으로 발견되는 N-Terminal End Region (NTER)을 포함하는 펩타이드의 생체내에서의 ErmSF 활성 억제 효과 검색)

  • Jin, Hyung-Jong
    • Korean Journal of Microbiology
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    • v.47 no.3
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    • pp.200-208
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    • 2011
  • Most problematic antibiotic resistance mechanism for MLS (macrolide-lincosamide-streptogramn B) antibiotics encountered in clinical practice is mono- or dimethylation of specific adenine residue at 2058 (E. coli coordinate) of 23S rRNA which is performed by Erm (erythromycin ribosome resistance) protein through which bacterial ribosomes reduce the affinity to the antibiotics and become resistant to them. ErmSF is one of the four gene products produced by Streptomyces fradiae to be resistant to its own antibiotic, tylosin. Unlike other Erm proteins, ErmSF harbors idiosyncratic long N-terminal end region (NTER) 25% of which is comprised of arginine well known to interact with RNA. Furthermore, NTER was found to be important because when it was truncated, most of the enzyme activity was lost. Based on these facts, capability of NTER peptide to inhibit the enzymatic activity of ErmSF was sought. For this, expression system for two different proteins to be expressed in one cell was developed. In this system, two plasmids, pET23b and pACYC184 have unique replication origins to be compatible with each other in a cell. And expression system harboring promoter, ribosome binding site and transcription termination signal is identical but disparate amount of protein could be expressed according to the copy number of each vector, 15 for pACYC and 40 for pET23b. Expression of NTER peptide in pET23b together with ErmSF in pACYC 184 in E. coli successfully gave more amounts of NTER than ErmSF but no inhibitory effects were observed suggesting that there should be dynamicity in interaction between ErmSF and rRNA rather than simple and fixed binding to each other in methylation of 23S rRNA by ErmSF.

3D Point Cloud Reconstruction Technique from 2D Image Using Efficient Feature Map Extraction Network (효율적인 feature map 추출 네트워크를 이용한 2D 이미지에서의 3D 포인트 클라우드 재구축 기법)

  • Kim, Jeong-Yoon;Lee, Seung-Ho
    • Journal of IKEEE
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    • v.26 no.3
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    • pp.408-415
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    • 2022
  • In this paper, we propose a 3D point cloud reconstruction technique from 2D images using efficient feature map extraction network. The originality of the method proposed in this paper is as follows. First, we use a new feature map extraction network that is about 27% efficient than existing techniques in terms of memory. The proposed network does not reduce the size to the middle of the deep learning network, so important information required for 3D point cloud reconstruction is not lost. We solved the memory increase problem caused by the non-reduced image size by reducing the number of channels and by efficiently configuring the deep learning network to be shallow. Second, by preserving the high-resolution features of the 2D image, the accuracy can be further improved than that of the conventional technique. The feature map extracted from the non-reduced image contains more detailed information than the existing method, which can further improve the reconstruction accuracy of the 3D point cloud. Third, we use a divergence loss that does not require shooting information. The fact that not only the 2D image but also the shooting angle is required for learning, the dataset must contain detailed information and it is a disadvantage that makes it difficult to construct the dataset. In this paper, the accuracy of the reconstruction of the 3D point cloud can be increased by increasing the diversity of information through randomness without additional shooting information. In order to objectively evaluate the performance of the proposed method, using the ShapeNet dataset and using the same method as in the comparative papers, the CD value of the method proposed in this paper is 5.87, the EMD value is 5.81, and the FLOPs value is 2.9G. It was calculated. On the other hand, the lower the CD and EMD values, the better the accuracy of the reconstructed 3D point cloud approaches the original. In addition, the lower the number of FLOPs, the less memory is required for the deep learning network. Therefore, the CD, EMD, and FLOPs performance evaluation results of the proposed method showed about 27% improvement in memory and 6.3% in terms of accuracy compared to the methods in other papers, demonstrating objective performance.

Development of disaster severity classification model using machine learning technique (머신러닝 기법을 이용한 재해강도 분류모형 개발)

  • Lee, Seungmin;Baek, Seonuk;Lee, Junhak;Kim, Kyungtak;Kim, Soojun;Kim, Hung Soo
    • Journal of Korea Water Resources Association
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    • v.56 no.4
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    • pp.261-272
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    • 2023
  • In recent years, natural disasters such as heavy rainfall and typhoons have occurred more frequently, and their severity has increased due to climate change. The Korea Meteorological Administration (KMA) currently uses the same criteria for all regions in Korea for watch and warning based on the maximum cumulative rainfall with durations of 3-hour and 12-hour to reduce damage. However, KMA's criteria do not consider the regional characteristics of damages caused by heavy rainfall and typhoon events. In this regard, it is necessary to develop new criteria considering regional characteristics of damage and cumulative rainfalls in durations, establishing four stages: blue, yellow, orange, and red. A classification model, called DSCM (Disaster Severity Classification Model), for the four-stage disaster severity was developed using four machine learning models (Decision Tree, Support Vector Machine, Random Forest, and XGBoost). This study applied DSCM to local governments of Seoul, Incheon, and Gyeonggi Province province. To develop DSCM, we used data on rainfall, cumulative rainfall, maximum rainfalls for durations of 3-hour and 12-hour, and antecedent rainfall as independent variables, and a 4-class damage scale for heavy rain damage and typhoon damage for each local government as dependent variables. As a result, the Decision Tree model had the highest accuracy with an F1-Score of 0.56. We believe that this developed DSCM can help identify disaster risk at each stage and contribute to reducing damage through efficient disaster management for local governments based on specific events.

Video Analysis System for Action and Emotion Detection by Object with Hierarchical Clustering based Re-ID (계층적 군집화 기반 Re-ID를 활용한 객체별 행동 및 표정 검출용 영상 분석 시스템)

  • Lee, Sang-Hyun;Yang, Seong-Hun;Oh, Seung-Jin;Kang, Jinbeom
    • Journal of Intelligence and Information Systems
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    • v.28 no.1
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    • pp.89-106
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    • 2022
  • Recently, the amount of video data collected from smartphones, CCTVs, black boxes, and high-definition cameras has increased rapidly. According to the increasing video data, the requirements for analysis and utilization are increasing. Due to the lack of skilled manpower to analyze videos in many industries, machine learning and artificial intelligence are actively used to assist manpower. In this situation, the demand for various computer vision technologies such as object detection and tracking, action detection, emotion detection, and Re-ID also increased rapidly. However, the object detection and tracking technology has many difficulties that degrade performance, such as re-appearance after the object's departure from the video recording location, and occlusion. Accordingly, action and emotion detection models based on object detection and tracking models also have difficulties in extracting data for each object. In addition, deep learning architectures consist of various models suffer from performance degradation due to bottlenects and lack of optimization. In this study, we propose an video analysis system consists of YOLOv5 based DeepSORT object tracking model, SlowFast based action recognition model, Torchreid based Re-ID model, and AWS Rekognition which is emotion recognition service. Proposed model uses single-linkage hierarchical clustering based Re-ID and some processing method which maximize hardware throughput. It has higher accuracy than the performance of the re-identification model using simple metrics, near real-time processing performance, and prevents tracking failure due to object departure and re-emergence, occlusion, etc. By continuously linking the action and facial emotion detection results of each object to the same object, it is possible to efficiently analyze videos. The re-identification model extracts a feature vector from the bounding box of object image detected by the object tracking model for each frame, and applies the single-linkage hierarchical clustering from the past frame using the extracted feature vectors to identify the same object that failed to track. Through the above process, it is possible to re-track the same object that has failed to tracking in the case of re-appearance or occlusion after leaving the video location. As a result, action and facial emotion detection results of the newly recognized object due to the tracking fails can be linked to those of the object that appeared in the past. On the other hand, as a way to improve processing performance, we introduce Bounding Box Queue by Object and Feature Queue method that can reduce RAM memory requirements while maximizing GPU memory throughput. Also we introduce the IoF(Intersection over Face) algorithm that allows facial emotion recognized through AWS Rekognition to be linked with object tracking information. The academic significance of this study is that the two-stage re-identification model can have real-time performance even in a high-cost environment that performs action and facial emotion detection according to processing techniques without reducing the accuracy by using simple metrics to achieve real-time performance. The practical implication of this study is that in various industrial fields that require action and facial emotion detection but have many difficulties due to the fails in object tracking can analyze videos effectively through proposed model. Proposed model which has high accuracy of retrace and processing performance can be used in various fields such as intelligent monitoring, observation services and behavioral or psychological analysis services where the integration of tracking information and extracted metadata creates greate industrial and business value. In the future, in order to measure the object tracking performance more precisely, there is a need to conduct an experiment using the MOT Challenge dataset, which is data used by many international conferences. We will investigate the problem that the IoF algorithm cannot solve to develop an additional complementary algorithm. In addition, we plan to conduct additional research to apply this model to various fields' dataset related to intelligent video analysis.

Application of spatiotemporal transformer model to improve prediction performance of particulate matter concentration (미세먼지 예측 성능 개선을 위한 시공간 트랜스포머 모델의 적용)

  • Kim, Youngkwang;Kim, Bokju;Ahn, SungMahn
    • Journal of Intelligence and Information Systems
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    • v.28 no.1
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    • pp.329-352
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    • 2022
  • It is reported that particulate matter(PM) penetrates the lungs and blood vessels and causes various heart diseases and respiratory diseases such as lung cancer. The subway is a means of transportation used by an average of 10 million people a day, and although it is important to create a clean and comfortable environment, the level of particulate matter pollution is shown to be high. It is because the subways run through an underground tunnel and the particulate matter trapped in the tunnel moves to the underground station due to the train wind. The Ministry of Environment and the Seoul Metropolitan Government are making various efforts to reduce PM concentration by establishing measures to improve air quality at underground stations. The smart air quality management system is a system that manages air quality in advance by collecting air quality data, analyzing and predicting the PM concentration. The prediction model of the PM concentration is an important component of this system. Various studies on time series data prediction are being conducted, but in relation to the PM prediction in subway stations, it is limited to statistical or recurrent neural network-based deep learning model researches. Therefore, in this study, we propose four transformer-based models including spatiotemporal transformers. As a result of performing PM concentration prediction experiments in the waiting rooms of subway stations in Seoul, it was confirmed that the performance of the transformer-based models was superior to that of the existing ARIMA, LSTM, and Seq2Seq models. Among the transformer-based models, the performance of the spatiotemporal transformers was the best. The smart air quality management system operated through data-based prediction becomes more effective and energy efficient as the accuracy of PM prediction improves. The results of this study are expected to contribute to the efficient operation of the smart air quality management system.

A study on age distortion reduction in facial expression image generation using StyleGAN Encoder (StyleGAN Encoder를 활용한 표정 이미지 생성에서의 연령 왜곡 감소에 대한 연구)

  • Hee-Yeol Lee;Seung-Ho Lee
    • Journal of IKEEE
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    • v.27 no.4
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    • pp.464-471
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    • 2023
  • In this paper, we propose a method to reduce age distortion in facial expression image generation using StyleGAN Encoder. The facial expression image generation process first creates a face image using StyleGAN Encoder, and changes the expression by applying the learned boundary to the latent vector using SVM. However, when learning the boundary of a smiling expression, age distortion occurs due to changes in facial expression. The smile boundary created in SVM learning for smiling expressions includes wrinkles caused by changes in facial expressions as learning elements, and it is determined that age characteristics were also learned. To solve this problem, the proposed method calculates the correlation coefficient between the smile boundary and the age boundary and uses this to introduce a method of adjusting the age boundary at the smile boundary in proportion to the correlation coefficient. To confirm the effectiveness of the proposed method, the results of an experiment using the FFHQ dataset, a publicly available standard face dataset, and measuring the FID score are as follows. In the smile image, compared to the existing method, the FID score of the smile image generated by the ground truth and the proposed method was improved by about 0.46. In addition, compared to the existing method in the smile image, the FID score of the image generated by StyleGAN Encoder and the smile image generated by the proposed method improved by about 1.031. In non-smile images, compared to the existing method, the FID score of the non-smile image generated by the ground truth and the method proposed in this paper was improved by about 2.25. In addition, compared to the existing method in non-smile images, it was confirmed that the FID score of the image generated by StyleGAN Encoder and the non-smile image generated by the proposed method improved by about 1.908. Meanwhile, as a result of estimating the age of each generated facial expression image and measuring the estimated age and MSE of the image generated with StyleGAN Encoder, compared to the existing method, the proposed method has an average age of about 1.5 in smile images and about 1.63 in non-smile images. Performance was improved, proving the effectiveness of the proposed method.

Comparative study of flood detection methodologies using Sentinel-1 satellite imagery (Sentinel-1 위성 영상을 활용한 침수 탐지 기법 방법론 비교 연구)

  • Lee, Sungwoo;Kim, Wanyub;Lee, Seulchan;Jeong, Hagyu;Park, Jongsoo;Choi, Minha
    • Journal of Korea Water Resources Association
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    • v.57 no.3
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    • pp.181-193
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    • 2024
  • The increasing atmospheric imbalance caused by climate change leads to an elevation in precipitation, resulting in a heightened frequency of flooding. Consequently, there is a growing need for technology to detect and monitor these occurrences, especially as the frequency of flooding events rises. To minimize flood damage, continuous monitoring is essential, and flood areas can be detected by the Synthetic Aperture Radar (SAR) imagery, which is not affected by climate conditions. The observed data undergoes a preprocessing step, utilizing a median filter to reduce noise. Classification techniques were employed to classify water bodies and non-water bodies, with the aim of evaluating the effectiveness of each method in flood detection. In this study, the Otsu method and Support Vector Machine (SVM) technique were utilized for the classification of water bodies and non-water bodies. The overall performance of the models was assessed using a Confusion Matrix. The suitability of flood detection was evaluated by comparing the Otsu method, an optimal threshold-based classifier, with SVM, a machine learning technique that minimizes misclassifications through training. The Otsu method demonstrated suitability in delineating boundaries between water and non-water bodies but exhibited a higher rate of misclassifications due to the influence of mixed substances. Conversely, the use of SVM resulted in a lower false positive rate and proved less sensitive to mixed substances. Consequently, SVM exhibited higher accuracy under conditions excluding flooding. While the Otsu method showed slightly higher accuracy in flood conditions compared to SVM, the difference in accuracy was less than 5% (Otsu: 0.93, SVM: 0.90). However, in pre-flooding and post-flooding conditions, the accuracy difference was more than 15%, indicating that SVM is more suitable for water body and flood detection (Otsu: 0.77, SVM: 0.92). Based on the findings of this study, it is anticipated that more accurate detection of water bodies and floods could contribute to minimizing flood-related damages and losses.