• IMMUNE NETWORK
• /
• v.3 no.1
• /
• pp.16-22
• /
• 2003

Background: The S100A2 gene, also known as S100L or CaN19, encodes a protein comprised of 99-amino acids, is a member of the calcium-binding proteins of EF-hand family. According to a recent study, this gene was over-expressed in several early and malignant carcinomas compared to normal tissues. To elucidate the role of S100A2 protein in the process during carcinogenesis, production of monoclonal antibody specific to the protein is essential. Methods: First, cDNA sequence coding for ORF region of human S100A2 gene was amplified and cloned into an expression vector to produce GST fusion protein. Recombinant S100A2 protein and subsequently, monoclonal antibody to the protein were produced. The specificity of anti-S100A2 monoclonal antibody was confirmed by immunoblot analysis of cross reactivity to other recombinant proteins of S100A family (GST-S100A1, GST-S100A4 and GST-S100A6). To confirm the relation of S100A2 to cervical carcinogenesis, S100A2 protein in early cervical carcinoma tissue was immunostained using the monoclonal antibody. Results: GST-S100A2 recombinant protein was purified by affinity chromatography and then fusion protein was cleaved and S100A2 protein was isolated. The monoclonal antibody (KK0723; Korean patent pending #2001-30294) to the protein was produced and the antibody did not react with other members of EF-hand family proteins such as S100A1, S100A4 and S100A6. Conclusion: These data suggest that anti-S100A2 monoclonal antibody produced in this study can be very useful for the early detection of cervical carcinoma and elucidation of mechanism during the early cervical carcinogenesis.

• 한국생물공학회:학술대회논문집
• /
• 2003.04a
• /
• pp.40-44
• /
• 2003

Soil-borne infectious disease including Pythium aphanidermatum and Rhizoctonia solani causes severe damage to plants, such as cucumber. This soil-borne infectious disease was not controlled effectively by chemical pesticide. Since these diseases spread through the soil, chemical agents are usually ineffective. Instead, biological control, including antagonistic microbe can be used as a preferred control method. An efficient method was developed to select an antagonistic strain to be used as a biological control agent strain. In this new method, surface tension reduction potential of an isolate was included in the ‘decision factor’ in addition to the other factors, such as growth rate, and pathogen inhibition rate. Considering these 3 decision factors by a statistical method, an isolate from soil was selected and was identified as Bacillus sp. GB16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth was observed when Bacillus sp. GB16 was used. Therefore this strain was considered as plant growth promoting rhizobacteria (PGPR). The action of surface tension reducing component was deduced as the enhancement of wetting, spreading, and residing of antagonistic strain in the rhizosphere. This result showed that new selection method was significantly effective in selecting the best antagonistic strain for biological control of soil-borne infectious plant pathogen. The antifungal substances against P. aphanidermatum and R. solani were partially purified from the culture filtrates of Bacillus sp. GB16. In this study, lipopeptide possessing antifungal activity was isolated from Bacillus sp. GB16 cultures by various purification procedures and was identified as a surfactin-like lipopeptide based on the Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), high performance liquid chromatography mass spectroscopy (HPLC-MS), and quadrupole time-of-flight (Q-TOF) ESI-MS/MS data. The lipopeptide, named GB16-BS, completely inhibited the growth of Pythium aphanidermatum, Rhizoctonia solani, Penicillium sp., and Botrytis cineria at concentrations of 10 and 50 mg/L, respectively. A novel method to prevent the foaming and to provide oxygen was developed. During the production of surface active agent, such as lipopeptide (surfactin), large amount of foam was produced by aeration. This resulted in the carryover of cells to the outside of the fermentor, which leads to the significant loss of cells. Instead of using cell-toxic antifoaming agents, low amount of hydrogen peroxide was added. Catalase produced by cells converted hydrogen peroxide into oxygen and water. Also addition of corn oil as an oxygen vector as well as antifoaming agent was attempted. In addition, Ca-stearate, a metal soap, was added to enhance the antifoam activity of com oil. These methods could prevent the foaming significantly and maintained high dissolved oxygen in spite of lower aeration and agitation. Using these methods, high cell density, could be achieved with increased lipopeptide productivity. In conclusion to produce an effective biological control agent for soil-borne infectious disease, following strategies were attempted i) effective screening of antagonist by including surface tension as an important decision factor ii) identification of antifungal compound produced from the isolated strain iii) novel oxygenation by $H_2O_2-catalase$ with vegetable oil for antifungal lipopeptide production.

• Korean Journal of Construction Engineering and Management
• /
• v.17 no.2
• /
• pp.39-48
• /
• 2016

According to the order receipt report over the past 10 years the overseas construction business total trades were 54.05% and 68.09% done by the Middle East and other industrial facilities respectively. In the Middle East based on data collected in 2014 the country with most overseas construction business is Saudi Arabia with 34.10%, and the industrial facility occupies the larger share with 56%. Overseas construction business is suffering from a reduction in work orders in the Middle East because of the recent oil price reduction. At this point of view, it is necessary to consider economy fluctuation for the diversification of construction type and work orders. This study analyzed, focusing in Saudi Arabia how the economical fluctuations of nations of progress can affect overseas construction business' trade orders. The analysis results demonstrated that most construction types depends on GDP, investment fund. Also industrial facility can be substituted with Architecture and civil engineering. This work is expected to be used as a basis for trade order amount maintenance and construction type diversification.

• Journal of the Korean Society for Library and Information Science
• /
• v.40 no.3
• /
• pp.175-190
• /
• 2006

For the recent several years, there has been much interest in lexical semantic network However it seems to be very difficult to evaluate the effectiveness and correctness of it and invent the methods for applying it into various problem domains. In order to offer the fundamental ideas about how to evaluate and utilize lexical semantic networks, we developed two automatic vol·d clustering systems, which are called system A and system B respectively. 68.455.856 words were used to learn both systems. We compared the clustering results of system A to those of system B which is extended by the lexical-semantic network. The system B is extended by reconstructing the feature vectors which are used the elements of the lexical-semantic network of 3.656 '-ha' verbs. The target data is the 'multilingual Word Net-CoroNet'. When we compared the accuracy of the system A and system B, we found that system B showed the accuracy of 46.6% which is better than that of system A. 45.3%.

• International Commerce and Information Review
• /
• v.13 no.3
• /
• pp.77-95
• /
• 2011

This paper investigates the relationship between export and economic variables such as trade insurance, world economy activity, relative price, unemployment rate, exchange rate volatility, using monthly data. I employ Johansen cointegration methodology since the model must be stationary to avoid the spurious results. The results indicate that there is a long-run relationship between export and variables. Also, the empirical analysis of cointegrating vector using the CCR, DOLS, FMOLS reveals that the increases of trade insurance has positive relations and the increases of exchange rate volatility have negative relations with export. Especially, DOLS based on Monte Carlo simulations, of this estimator being superior in small samples compared to a number of alternative estimators, as well as being able not only to accommodate higher orders of integration but also to account for possible simultaneity within regressors of a potential system. This paper also applies impulse-response functions to get the additional information regarding the responses of the export to the shocks of the variables. The result indicates that export positively to trade insurance and then decay fast compare with exchange rate volatility. Consequently, trade insurance plays the role of trade policy for export promotion in Korea. Whereas, increase of exchange risk result in reduction of export. Therefore, the support of trade insurance should be expanded and the stabilization of the foreign exchange market must be done for the export promotion.

• The KIPS Transactions:PartB
• /
• v.11B no.1
• /
• pp.27-34
• /
• 2004

This paper proposes a method to trace and interpret a moving object based on the information which can be directly obtained from MPEG-2 compressed video stream without decoding process. In the proposed method, the motion flow is constructed from the motion vectors included in compressed video. We calculate the amount of pan, tilt, and zoom associated with camera operations using generalized Hough transform. The local object motion can be extracted from the motion flow after the compensation with the parameters related to the global camera motion. Initially, a moving object to be traced is designated by user via bounding box. After then automatic tracking Is performed based on the accumulated motion flows according to the area contributions. Also, in order to reduce the cumulative tracking error, the object area is reshaped in the first I-frame of a GOP by matching the DCT coefficients. The proposed method can improve the computation speed because the information can be directly obtained from the MPEG-2 compressed video, but the object boundary is limited by macro-blocks rather than pixels. Also, the proposed method is proper for approximate object tracking rather than accurate tracing of an object because of limited information available in the compressed video data.

• The KIPS Transactions:PartB
• /
• v.9B no.6
• /
• pp.853-862
• /
• 2002

Face Detection can be defined as follows : Given a digitalized arbitrary or image sequence, the goal of face detection is to determine whether or not there is any human face in the image, and if present, return its location, direction, size, and so on. This technique is based on many applications such face recognition facial expression, head gesture and so on, and is one of important qualify factors. But face in an given image is considerably difficult because facial expression, pose, facial size, light conditions and so on change the overall appearance of faces, thereby making it difficult to detect them rapidly and exactly. Therefore, this paper proposes fast and exact face detection which overcomes some restrictions by using neural network. The proposed system can be face detection irrelevant to facial expression, background and pose rapidily. For this. face detection is performed by neural network and detection response time is shortened by reducing search region and decreasing calculation time of neural network. Reduced search region is accomplished by using skin color segment and frame difference. And neural network calculation time is decreased by reducing input vector sire of neural network. Principle Component Analysis (PCA) can reduce the dimension of data. Also, pose estimates in extracted facial image and eye region is located. This result enables to us more informations about face. The experiment measured success rate and process time using the Squared Mahalanobis distance. Both of still images and sequence images was experimented and in case of skin color segment, the result shows different success rate whether or not camera setting. Pose estimation experiments was carried out under same conditions and existence or nonexistence glasses shows different result in eye region detection. The experiment results show satisfactory detection rate and process time for real time system.

• Cartoon and Animation Studies
• /
• s.14
• /
• pp.37-51
• /
• 2008

The computer has become an indispensable tool for animation works. However if you don't understand the characteristics of the computer and its software, you might not have the result satisfying your efforts. The incorrect understanding of image format sometimes causes it. Habitually image formats are selected usually for most of works but there is a distinct difference among those image formats while the efficient usages of them are different from each other. For your more efficient work therefore, you need to identify the characteristics of various kinds of image format used mostly for animation works. First I took a look at the theories of the lossy compression and lossless compression, which are two types of data compression widely used in the whole parts of computer world and the difference between bitmap method and vector method, which are respectably different in terms of the way of expressing images and finally the 24 bit true color and 8 bits alpha channel. Based on those characteristics, I have analyzed the functional difference among image formats used between various types of animation works such as 2D, 3D, composing and editing and also the benefits and weakness of them. Additionally I've proved it is wrong that the JPEG files consume a small space in computer work. In conclusion, I suggest the TIF format as the most efficient format for whatever it is editing, composing, 3D and 2D in considering capacity, function and image quality and also I'd like to recommend PSD format which has compatibility and excellent function, since the Adobe educational programs are used a lot for the school education. I hope this treatise to contribute to your right choice of image format in school education and practical works.

• The Korean Journal of Physiology and Pharmacology
• /
• v.9 no.5
• /
• pp.275-282
• /
• 2005

By using differential display, we identified one of the genes encoding the multi-subunit complex protein V-ATPase, c subunit gene (ATP6L), and showed alterations of the gene expression by oxidative stresses. Expression of the ATP6L gene in Neuro-2A cells was increased by the treatment with $H_2O_2$ and incubation in hypoxic chamber, implying that the expression of the ATP6L gene is regulated by oxidative stresses. To examine mechanisms involved in the regulation of the gene expression by oxidative stresses, the transcriptional activity of the rat ATP6L promoter was studied. Transcription initiation site was determined by primer extension analysis and DNA sequencing, and promoter of the rat ATP6L and its deletion clones were constructed in reporter assay vector. Significant changes of the promoter activities in Neuro-2A cells were observed in two regions within the proximal 1 kbp promoter, and one containing a suppressor was in -195 to -220, which contains GC box that is activated by binding of Sp1 protein. The suppression of promoter activity was lost in mutants of the GC box. We confirmed by electrophoretic mobility shift and supershift assays that Sp1 protein specifically binds to the GC box. The promoter activity was not changed by the $H_2O_2$ treatment and incubation in hypoxic chamber, however, $H_2O_2$ increased the stability of ATP6L mRNA. These data suggest that the expression of the ATP6L gene by oxidative stresses is regulated at posttranscriptional level, whereas the GC box is important in basal activities of the promoter.

• Microbiology and Biotechnology Letters
• /
• v.31 no.2
• /
• pp.117-123
• /
• 2003

For screening of autoregulator receptor gene from Saccharopolyspora erythraea, PCR was performed with primers of receptor gene designed on the basis of amino acid sequences of autoregulator receptor proteins with known function. PCR products were subcloned into the BamHI site of pUC19 and transformed into the E. coli DH5$\alpha$. The isolated plasmid from transformant contained the fragment of 120 bp, which was detected on 2% gel after BamHI treatment. The insert, 120 bp PCR product, was confirmed as the expected internal segment of gene encoding autoregulator receptor protein by sequencing. Southern and colony hybridization using Saccha. erythraea chromosomal DNA were performed with the insert as probe. The plasmid (pEsg) having 3.2 kbp SacI DNA fragment from Saccha. erythraea is obtained. The 3.2 kbp SacI DNA fragment was sequenced by the dye terminator sequencing. The nucleotide sequence data was analyzed with GENETYX-WIN (ver 3.2) computer program and DNA database. frame analyses of the nucleotide sequence revealed a gene encoding autoregulator receptor protein which is a region including KpnI and SalI sites on 3.2 kbp SacI DNA fragment. The autoregulator receptor protein consisting of 205 amino acid was named EsgR by author. In comparison with known autoregulator receptor proteins, homology of EsgR showed above 30%.