• Clinical and Experimental Pediatrics
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• 제53권6호
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• pp.680-687
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• 2010

Patients with congenital heart diseases (CHD) are confronted with early- and late-onset complications, such as conduction disorders, arrhythmias, myocardial dysfunction, altered coronary flow, and ischemia, throughout their lifetime despite successful hemodynamic and/or anatomical correction. Rhythm disturbance is a well-known and increasingly frequent cause of morbidity and mortality in patients with CHD. Predisposing factors to rhythm disturbances include underlying cardiac defects, hemodynamic changes as part of the natural history, surgical repair and related scarring, and residual hemodynamic abnormalities. Acquired factors such as aging, hypertension, diabetes, obesity, and others may also contribute to arrhythmogenesis in CHD. The first step in evaluating arrhythmias in CHD is to understand the complex anatomy and to find predisposing factors and hemodynamic abnormalities. A practical stepwise approach can lead to diagnosis and prompt appropriate interventions. Electrophysiological assessment and management should be done with integrated care of the underlying heart defects and hemodynamic abnormalities. Catheter ablation and arrhythmia surgery have been increasingly applied, showing increasing success rates with technological advancement despite complicated arrhythmia circuits in complex anatomy and the difficulty of access. Correction of residual hemodynamic abnormalities may be critical in the treatment of arrhythmia in patients with CHD.

• The Korean Journal of Physiology and Pharmacology
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• 제20권1호
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• pp.53-62
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• 2016

Mesenchymal stem cells (MSCs) in the bone marrow and other somatic tissues reside in an environment with relative low oxygen tension. Cobalt chloride ($CoCl_2$) can mimic hypoxic conditions through transcriptional changes of some genes including hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) and vascular endothelial growth factor (VEGF). This study evaluated the potential role of $CoCl_2$ preconditioning on multi-lineage differentiation of C3H/10T1/2, a murine MSC line to understand its possible molecular mechanisms in vitro. $CoCl_2$ treatment of MSCs markedly increased HIF-$1{\alpha}$ and VEGF mRNA, and protein expression of HIF-$1{\alpha}$. Temporary preconditioning of MSCs with $CoCl_2$ induced up-regulation of osteogenic markers including alkaline phosphatase, osteocalcin, and type I collagen during osteogenic differentiation, followed by enhanced mineralization. $CoCl_2$ also increased chondrogenic markers including aggrecan, sox9, and type II collagen, and promoted chondrocyte differentiation. $CoCl_2$ suppressed the expression of adipogenic markers including $PPAR{\gamma}$, aP2, and $C/EBP{\alpha}$, and inhibited adipogenesis. Temporary preconditioning with $CoCl_2$ could affect the multi-lineage differentiation of MSCs.

• Food Science and Biotechnology
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• 제14권6호
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• pp.813-817
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• 2005

We investigated the effect of a viscous substance from chongkukjang (chongkukjang mucilage) on immunohistochemical reactions in rat gastrointestinal (GI) tracts, Rats fed a steady diet of chongkukjang mucilage showed an increase in the immunoreactive densities of gastrin and serotonin in the pyloric region of their stomachs and duodenal villi, The number of gastrin and serotonin immunoreactive cells was significantly higher in the experimental group than in the control group. Feeding on dietary chongkukjang mucilage increased the immunohistochemical densities of $CD4^+$ and $CD8^+$ lymphocytes in the mucosa and submucosa of the rats' gastroduodenal region. The universal nitric oxide synthase (uNOS)-immunoreactive neurons and nerve fibers were strongly stained in the vascular walls of the submucosa and myenteric plexus in rats fed the test diet. The results indicate that the intake of chongkukjang mucilage could increase mucosal immune activity, gastrointestinal motility, and blood circulation in the GI tract.

• BMB Reports
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• 제41권12호
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• pp.833-839
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• 2008

Angiogenesis in tumors is driven by multiple growth factors that activate receptor tyrosine kinases. An important driving force of angiogenesis in solid tumors is signaling through vascular endothelial growth factor (VEGF) and its receptors (VEGFRs). Angiogenesis inhibitors that target this signaling pathway are now in widespread use for the treatment of cancer. However, when used alone, inhibitors of VEGF/VEGFR signaling do not destroy all blood vessels in tumors and do not slow the growth of most human cancers. VEGF/VEGFR signaling inhibitors are, therefore, used in combination with chemotherapeutic agents or radiation therapy. Additional targets for inhibiting angiogenesis would be useful for more efficacious treatment of cancer. One promising target is the signaling pathway of hepatocyte growth factor (HGF) and its receptor (HGFR, also known as c-Met), which plays important roles in angiogenesis and tumor growth. Inhibitors of this signaling pathway have been shown to inhibit angiogenesis in multiple in vitro and in vivo models. The HGF/c-Met signaling pathway is now recognized as a promising target in cancer by inhibiting angiogenesis, tumor growth, invasion, and metastasis.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제45권1호
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• pp.43-47
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• 2019

The characteristics of extra-nasopharyngeal angiofibromas tend to be different from angiofibromas of the nasopharynx according to patient gender, patient age, prevalence, affected site, pathogenesis, and clinical and epidemiological features. We report a case of an extra-nasopharyngeal angiofibroma in a 28-year-old man referred to the ENT Clinic for right-sided epistaxis, airflow impairment and nasal swelling. The right nostril was completely occluded works by a reddish-yellow mass that bled easily. The computed tomography scan revealed an "inhomogeneous solid lesion in the nasal fossa". With the patient under general anesthesia, the formation in the anterior portion of the right side of the nasal septum was removed up to its vascular base. Although electrical cauterization efficiently controlled the bleeding, we abraded the sub-perichondral area to prevent further bleeding as well as recurrence. The histological exam report confirmed the diagnosis of angiofibroma. As in our case, epistaxis is commonly the presenting sign of angiofibroma. Yet its onset was peculiar, given that the bleeding started with a low impact trauma. The nasal swelling was also a relevant feature as well as the breathing impairment. Although uncommon, nasal septal angiofibromas should considered in patients with epistaxis.

• Journal of Korean Neurosurgical Society
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• 제62권2호
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• pp.175-182
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• 2019

Objective : Aberrant right subclavian artery (ARSA) is a rare anatomical variant of the origin of the right subclavian artery. ARSA is defined as the right subclavian artery originating as the final branch of the aortic arch. The purpose of this study is to determine the prevalence and the anatomy of ARSA evaluated with computed tomography (CT) angiography. Methods : CT angiography was performed in 3460 patients between March 1, 2014 and November 30, 2015 and the results were analyzed. The origin of the ARSA, course of the vessel, possible inadvertent ARSA puncture site during subclavian vein catheterization, Kommerell diverticula, and associated vascular anomalies were evaluated. We used the literature to review the clinical importance of ARSA. Results : Seventeen in 3460 patients had ARSA. All ARSAs in 17 patients originated from the posterior aspect of the aortic arch and traveled along a retroesophageal course to the right thoracic outlet. All 17 ARSAs were located in the anterior portion from first to fourth thoracic vertebral bodies and were located near the right subclavian vein at the medial third of the clavicle. Only one of 17 patients presented with dysphagia. Conclusion : It is important to be aware ARSA before surgical approaches to upper thoracic vertebrae in order to avoid complications and effect proper treatment. In patients with a known ARSA, a right transradial approach for aortography or cerebral angiography should be changed to a left radial artery or transfemoral approach.

• 해부∙생물인류학
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• 제29권2호
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• pp.35-46
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• 2016

레오나르도 다빈치는 르네상스 시대의 천재 예술가이다. 그는 예술가와 과학자, 발명가로서 뛰어난 업적을 남겼고, 식물학과 수학, 지질학, 천문학, 기하학, 광학 등의 다양한 분야에서 최고의 반열에 올랐을 뿐 아니라 오늘날의 과학에까지 크게 기여하고 있다. 또한 레오나르도는 예술가와 과학자, 발명가, 철학자로도 잘 알려져 있으며, 사람과 동물들을 직접 해부하여 관찰한 후 많은 해부 그림들을 남긴 위대한 해부학자였다. 레오나르도가 해부학에 관심을 가졌던 이유는 - 화가는 해부학에 무지해서는 안 된다 - 라는 예술가의 관점에서 사람의 구조와 기능을 알기 위함이었다. 그는 사람 몸의 구조와 기능에 더욱 관심을 갖게 되었고, 시체를 구하기 어려운 상황에도 불구하고 많은 시체를 직접 해부하여 관찰하였다. 이러한 그의 해부학적 탐구와 심취로 말미암아 그는 동시대의 사람들보다 100년 이상 앞선 위대한 해부학 업적을 남겼다. 레오나르도가 남긴 뼈대와 근육, 혈관, 신경, 비뇨생식계통에 관한 1,800여 개의 해부 그림들은 높은 예술성과 함께 과학적으로도 매우 가치가 높은 걸작들이다. 이 연구의 목적은 레오나르도의 해부학 분야의 업적과 사고를 살펴보고, 해부학 분야의 선구자인 레오나르도의 위대한 업적을 오늘날 사람들에게 널리 알리고자 하는 것이다.

• 식물분류학회지
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• 제42권1호
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• pp.50-63
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• 2012

개나리족(Abeliophyllum: 1 sp., Forsythia: 12 spp.)과 향선나무족(Fontanesia: 2 spp.) 및 근연분류군인 Myxopyreae족(Myxopyrum: 5 spp.)을 포함한 총 20분류군의 엽병의 해부학적인 형질을 검토하고자 광학현미경(LM)을 이용하여 연구하였다. 모든 해부학적 형질은 엽병의 횡단면을 단부, 중앙부, 기부로 나누어 관찰하였고, 측정된 정량적 형질과 정성적 형질에 대해 자세히 비교하고 기재하였다. 세 가지 유형(침상, 프리즘형, 선정체)의 결정체가 Fontanesia속과 Myxopyrum속에서만 확인되었다. 미선나무속과 개나리속의 일부 분류군(Forsythia europaea, F. giraldiana, F. japonica)에서만 단세포성 비선모(uni-cellular non-glandular trichome)가 관찰되었다. 본 연구 결과에서는 엽병의 유관속 패턴을 크게 두 타입으로 구분하여 기재하였다. Type 1A: 결정체가 존재하지 않는 단순호형(Abeliophyllum, Forsythia), 1B: 결정체가 존재하는 단순호형(Myxopyrum), Type 2: 결정체가 존재하는 함입호형(Fontanesia). 또한 세부적인 해부학적 형질에 대해 자세히 기재하였고, 분류학적 중요성에 대해 논의하였다. 결론적으로 일부 엽병 해부학적 형질(예, 주유관속 패턴, 결정체의 유무)은 진단형질로서 유용할 뿐 아니라 최근의 분자계통학적 결과를 지지하였다.

• 식물분류학회지
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• 제39권1호
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• pp.4-11
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• 2009

한국에 자생하는 겨우살이과 4종, 겨우살이, 참나무겨우살이, 동백나무겨우살이, 꼬리겨우살이 등에 대해 잎과 줄기의 해부학적 특징과 기주식물별 2차 흡기구조의 해부학적 특징을 조사하였다. 그 결과 잎과 줄기의 해부학적 특징으로 한국산 겨우살이과 4 분류군을 종과 속 수준에서 구별할 수 있는 것으로 파악되었다. 잎에서는 향축면과 배축면의 축, 중앙맥에 발달된 유관속 형태, 후벽세포의 발달 여부 등이, 줄기에서는 횡단면에서 줄기의 전체적인 형태, 큐티클층의 발달 정도, 유관속의 배열, 후벽세포와 수의 발달 여부 등이 표징형질로 조사되었다. 기주별 2차 흡기의 특징을 확인하기 위해 참나무겨우살이와 동백나무겨우살이에서 각각 7종류 및 5종류의 기주식물을 대상으로 지상부 흡기 발달 정도, 종단면에서 2차 흡기의 모양과 플랜지 발달 여부, 기주내 침투 지역과 흡기 축의 발달 여부, 2차 흡기에서 흡기세포의 위치와 짧은 흡기 가도관의 발달 여부 등 형질을 조사하였다. 조사된 형질에서 참나무겨우살이와 동백나무겨우살이는 뚜렷하게 구별되었다. 한편, 기주식물의 강도와 흡기의 발달 정도에 따라 2차 흡기의 전체적인 형태가 기주별로 다소 차이가 있었으나, 침투 지역, 흡기 짧은 가도관 세포의 발달 등 정성적인 형질들은 기주와 발달 정도에 영향을 받지 않고 항상 일정하여 계통학적으로 의미 있는 특징으로 파악되었다.

• Applied Microscopy
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• 제27권3호
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• pp.333-346
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• 1997

The ischemia and reperfusion injury of the skeletal muscles is caused by generation of reactive oxygen during ischemia and reperfusion. It is well known that over 4 hours of ischemia injures the skeletal muscles irreversibly. The author has demonstrated the effects of SOD (superoxide dismutase), DMTU (dimethyl thiourea) and ischemic preconditioning on ultrastructural changes of the muscle fibers in the rectus femoris muscles after 4 hours of ischemia and 1 day and 3 days of reperfusion. A total of 72 healthy Sprague-Dawley rats weighing from 200 gm to 250 gm were used as experimental animals. Under urethane(1.15 g/kg, IP, 2 times) anesthesia, lower abdominal incision was done and the left common iliac artery was occluded by using vascular clamp for 4 hours. The left rectus femoris muscles were obtained at 1 and 3 days after the removal of vascular clamp. The SOD (15,000 unit/kg) or DMTU (500 mg/kg) were administered intraperitoneally at 1 hour before induction of ischemia. The ischemic preconditioned group underwent three episodes of 5 minutes occlusion and 5 minutes reperfusion followed by 4 hours of ischemia and 1 day and 3 days of reperfusion. The specimens were sliced into $1mm^3$ and prepared by routine methods for electron microscopic observation. All specimens were stained with uranyl acetate and lead citrate and then observed with Hitachi-600 transmission electron microscope. The results were as follows: 1. SOD or DMTU alone did not affect the ultrastructure of muscle fibers in the rectus femoris muscles. The electron density of mitochondrial matrix was decreased by ischemic preconditioning. 2. Dilated cisternae of sarcoplasmic reticulum, triad, mitochondria and the loss of myofilament in the sarcomere were observed in the 4 hours ischemia and 1 day reperfused rectus femoris muscles. Markedly changed sarcoplasmic reticulum, triad, disordered or loss of myofilament, indistinct A-band and I-band, and irregular electron lucent M -line and Z-line are seen in the 4 hours ischemia and 3 days reperfused rectus femoris muscles. 3. SOD reduced the changes of organelles in the muscle fibers of the 4 hours ischemia and 1 day reperfused rectus femoris muscles of the rats, but SOD did not affect the changes of muscle fibers in the 4 hours ischemia and 3 days reperfused muscles. On the other hand, DMTU markedly attenuated considerably the ultrastructural change of the 4 hours ischemia and 1 day or 3 days reperfused rectus femoris muscles. 4. By the ischemic preconditioning, the change was attenuated remarkably in the 4 hours ischemia and 1 day reperfused rectus femoris muscles. As the ischemic reperfused changes of muscle fibers were regenerated or recovered by ischemic preconditioning, the ultrastructures of them were similar to those of normal control in the 4 hours ischemia and 3 days reperfused rectus formoris muscles. Consequently, it is suggested that DMTU is stronger inhibitor to ischemic reperfused change than SOD. The ischemia and reperfusion-induced muscular damage is remarkably inhibited by ischemic preconditioning.