• Radiation Oncology Journal
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• 제12권2호
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• pp.129-141
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• 1994

Intracellular ions which have a major role in cellular function have been reported to affect repair of radiation damage. Recently it has been reported that ouabain sensitizes A549 tumor cellls but not CCL-120 normal cells to radiation. Ouabain inhibits the $Na^+-K^+$-pump rapidly thus it increases intracellular Na concentration, Vanadate which is distributed extensively in almost all living organisms is known to be a $Na^+-K^+$-ATPase inhibitors, This study was performed to see any change in radiosensitivity of tumor cell by vanadate and any role of $Na^+-K^+$ATPase in radiosensitization. Experiments have been carried out by pretreatment with vanadate in human cell line(A549, JMG) and mouse cell line(L1210, spleen). For the cell survival MTT assay was performed for A549 and JMC cells and frypan blue dye exclusion test for L120, and spleen cells. Measurements of $Na^+-K^+$-ATPase activity in control, vanadate treated cell, radiation treated cell (9 Gy for A549 and JMG, 2 Gy for L1201, spleen), and combined $10^{-6}M$ vanadate and radiation treated cells were done. The results were summerized as fellows. 1. L1210 cell was most radiosensitive, and spleen cell and JMG cell were intermediate, and A549 cell was least radiosensitive. 2. Mininum or no cytotoxicity was seen with vanadate below concentration of $10^{-6}M$. 3. In A549 cells there was a little change in radiosensitivity with treatment of vanadate. However radiation sensitization was shown in low dose level of radiation i. e. 2- Gy. In JMG cells no change in radiosensitivity was noted. Both L1210 and spleen cell had radiosensitization but change was greater in tumor cell. 4. $Na^+-K^+$-ATPase activity was inhibited significantly in tumor cell by treatment of vanadate. 5. Radiaiton itself inhibited $Na^+-K^+$-ATPase activity of tumor cell with high $Na^+-K^+$-ATPase concention. Increase in radiosensitivity by vanadate was closely associated with orginal $Na^+-K^+$-ATPase contents. From the above results vanadate had little cytotoxicity and it sensitized tumor cells to radiation. Inhibitory effect of vanadate on $Na^+-K^+$-ATPase activity might be one of the contributing factors for radiosensitization to tumor cells which has greater enzyme activity than that of normal cell. It was suggested vanadate could be used as a potential radiosensitizer for tumor cells.

• The Korean Journal of Physiology
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• 제18권2호
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• pp.163-169
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• 1984

Vanadate가 Na-K-ATPase의 최적 PH에 영향을 미치는 기전에 효소의 $Na^+$과 $K^+$의 결합부위가 관여하는지를 밝히기 위하여 가토 신피질 microsome을 이용하여 실험해서 다음과 같은 결과를 얻었다. 1) $5{\times}10^{-6}M$ vanadate 존재시 Na-K-ATPase의 추적 PH가 6.8로 이동하였다. 2) PH 6.8과 7.4에서 Na-K-ATPase활성의 비는 vanadate농도가 증가함에 따라 증가하였다. 3) Vanadate 존재시에도 용액의 $Na^+$농도가 50mM 이하일 때는 pH 7.4에서의 효소활성이 6.8보다 높았고 오히려 그 비는 대조군보다 증가하였다. 4) $K^+$농도가 7mM 이하일 때는 vanadate 존재시도 Na-K-ATPase의 활성이 PH 7.4에서 더 높았다. 5) 5mM $K^+$존재하에서도 vanadate농도를 $10^{-5}M$로 증가시키면 최적 pH는 6.8로 이동하였다. 6) $K^+-pNPPase$활성은 PH가 낮을수록 증가하였고 $10^{-7}M$ vanadate에 의한 억제 정도는 PH가 낮을수록 감소하였다. 이상의 결과로 볼때 vanadate 존재시 Na-K-ATPase의 최적 PH는 6.8로 이동하였으며 이것은 PH가 낮아 펄 때 나타나는 vanadate자체의 성질 변화 때문은 아니며 vanadate 존재시 pH가 효소의 $Na^+$과 $K^+$결합부위에 영향을 주어 나타나는 것으로 생각된다.

• Journal of Chest Surgery
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• 제21권2호
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• pp.291-298
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• 1988

The effect of Vanadate on the isometric contraction, membrane potential and intracellular calcium ion activities of rabbit myocardial cells were investigated, using calcium selective microelectrode, filled with neutral calcium ion carrier, ETH-1001. The resting tension, the membrane potential and the intracellular calcium ion activities were recorded in normal Tyrode solution and compared with those in the contracture induced by 10 mM Vanadate. The following results were obtained: 1. The dose-response relationship between the contraction of Vanadate and twitch tension showed near-maximum response in 5mM with no corresponding changes in action potential. 2. The resting tension increased up to the amplitude of a control twitch in 10mM Vanadate with resting membrane potential, hyperpolarized. 3. Increase in intracellular calcium ion activities proceeded the contracture by 10mM Vanadate which were restored to the control level in accordance with a decrease of intracellular calcium ion activities. 4. The amplitude of contractures by 10mM Vanadate were 90-120% of the control twitch tension in which the intracellular calcium ion activities were increased about 70 times from p Ca, 7.1 in the control to p Ca, 5.8 in contractures.

• Korean Chemical Engineering Research
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• 제44권2호
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• pp.114-120
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• 2006

중금속을 함유하여 환경문제를 야기하고 있는 무기안료를 대체할 환경 친화적 안료 제품을 개발할 목적으로, potassium vanadate와 bismuth nitrate의 수용액으로부터 bismuth vanadate 안료를 제조할 때 공정 변수가 생성되는 안료의 물성에 미치는 영향을 실험적으로 조사하였다. 두 수용액을 각각 일정한 유속으로 서서히 첨가한 후, 반응혼합물의 1차 pH와 2차 pH를 각각 4.5와 7.0~7.5로 하여 침전을 생성시켜 반응혼합물 속에서 3시간 이상 숙성하고, $400^{\circ}C$에서 3시간 이상 소성하면 0.90 이상의 은폐율과 ${\Delta}E^*$값 1.50 이하의 색상을 지닌 bismuth vanadate 안료를 제조할 수 있었다. 반응혼합물 중 Mo의 첨가량이 증가하면 안료의 은폐력이 증가하는 경향을 나타내었으나, 다른 미량 성분들의 영향은 그리 크지 않은 것으로 나타났다.

• 한국환경성돌연변이발암원학회지
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• 제21권2호
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• pp.156-163
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• 2001

Vanadium has been known as environmental polluants resulted from the burning of fossil fuels in nature. It led to toxic responses by prooxidant activity, inducing free radicals and the accumulation in the tissues. Recently, there has been growing interest in an essential nutritional requirement of vandium and especially the treatment of diabetes. But because of its strong toxicity, thease chemicals have narrow safety margin. In order to reduce metal toxicity, and increase absorption and biological activities, metal ions such as selenium and chromium were uptaken in yeast cells. In this study, Vanadium yeast was prepared by uptaking vanadate in yeast cells. Vanadate induced hematological and biochemical changes in the experimental rat blood were inhibited by the treatments of vanadium yeast. Lipid peroxidation and catalase activity were significantly increased in kidney and liver after a single intraperitoneal injection of vanadate to rats. However, these observations were apparently reduced in the vanadium yeast treated group. Vanadium amount in blood, kidney and liver after a single intraperitoneal injection of vanadium yeast was significantly reduced than that of vanadate treated group. In conclusion, vanadium yeast uptaken vanadate in yeast cells could reduce toxic effects of vanadate.

• 분석과학
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• 제12권3호
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• pp.184-189
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• 1999

수용액에서 vanadate와 N-benzyliminodiacetate (Bz-IDA) 리간드의 상호작용을 $^{51}V$ 핵자기공명분광법과 순환전압-전류법을 이용하여 조사하였다. $^{51}V$ 핵자기공명분광법 결과 바나듐(V)-Bz-IDA착물생성에 의한 두 개의 특정 피크(-515.5 ppm 및 -500.1 ppm)를 관찰할 수 있었다. 순환전압-전류법을 이용하여 얻어진 결과 바나듐(V)-Bz-IDA착물의 산화-환원파의 전위값은 각각 -0.05 V 및 -0.13 V에서 관찰하였다. 이로써 산화-환원반응은 가역적 일전자반응으로 추정되었다.

• The Korean Journal of Physiology
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• 제19권2호
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• pp.203-213
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• 1985

Na-K-ATPase의 일부로 알려져 있으며 intact cell에서도 쉽게 활성의 측정이 가능한 K-pNPPase활성을 신피질 절편에서 측정하여 ouabain에 영향을 받는 산소 소모량 및 세포내 전해질 함량, 그리고 microsome에서 측정한 Na-K-ATPase활성에 대한 ouabain 및 vanadate의 효과를 비교 관찰하여 다음과 같은 결과를 얻었다. 1) 신피질 절편에서 p-NPPase활성은 60분간 시간에 직선적으로 비례하여 증가하였고 이중 1 mM ouabain에 의해 억제되는 K-pNPPase활성은 악 55% 정도로서 전 incubation기간 중 일정한 값을 보였다. 2) K-pNPPase활성을 50% 억제하는 ouabain 및 vanadate의 농도는 각각 $7.9{\times}10^{-6}M$과 $1.3{\times}10^{-5}M$이었다. 3) 신피질 절편에서 측정한 전체 산소 소모량 중 1 mM ouabain에 의해 억제되는 부분(ouabain-sensitive fraction)에 대한 ouabain과 vanadate의 50% 억제 농도는 각각 $6.3{\times}10^{-6}M$과 $2.5{\times}10^{-5}M$로서 K-pNPPase에서의 값과 유사하였다. 4) Ouabain 및 vanadate에 의한 세포내 $Na^+$ 및 $K^+$함량의 변화 양상은 절편에서 측정한 K-pNPPase활성의 변화 양상과 유사하였다. 5) Microsome에서 측정한 Na-K-ATPase활성은 $10^{-3}M$의 ouabain이 나 vanadate에 의해 완전 억제되었으며 50% 억제 농도는 각각 $1.2{\times}10^{-6}M$과 $1.6{\times}10^{-6}M$로 1 mM ouabain에 영향을 받는 K-pNPPase 활성 및 산소 소모량을 50% 억제하는 농도보다 ouabain은 약 5배 vanadate는 약 15배 정도 낮았다. 이상의 결과로 미루어 가토 신피질 절편에서 측정한 K-pNPPase활성은 $Na^+-K^+$교환 점프의 활성을 나타내는 좋은 지표가 될 수 있으며 특히 세포막을 통한 물질 이동이나 세포의 기능에 대한 영향과 Na-K-ATPase활성에 대한 영향을 동시에 보고자 할 때는 microsome에서 측정한 Na-K-ATPase활성보다 더 이상적이라고 사료된다.

• The Korean Journal of Physiology
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• 제22권2호
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• pp.189-206
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• 1988

The effects of ouabain on the contractile and electrical activities were investigated in the isolated preparations of guinea-pig taenia coli, and compared with those of vanadate. Spontaneous contractions were recorded with force transducer, and electrical activites were measured by use of suction electrode, or single sucrose-gap technique. The contractions were induced by the electrical stimulation for 5 seconds every 1 minute with alternating current (60 Hz, 3.0 V/cm) through the platinum electrodes located in parallel with the long axis of the preparation. All experiments were performed in tris-buffered Tyrode solution which was aerated with $100%{\;}O_2$ and kept at $35^{\circ}C$. The results obtained were as follows: 1) Responses of spontaneous contractions to ouabain were concentration-dependent; $10^{-7}M$ ouabain caused a rise of basal tone. Above the concentration of $10^{-6}M$ ouabain, an initial increase followed by a decrease in tension was observed. 2) A continuous spike discharge was induced by the administration of $10^{-7}M$ ouabain. Above $10^{-6}M$ ouabain, a transient initial increase followed by a decrease in spike frequency and amplitude was produced, and finally membrane potential was sustained at a certain level without a spike discharge. 3) The characteristic response to $10^{-7}M$ ouabain was not blocked by the pretreatment with $10^{-7}M$ atropine. 4) The electrically induced contractions were completely suppressed at the concentration of $2{\times}10^{-7}M$ ouabain. These contractions were blocked more rapidly in paralled with the increase in ouabain concentration. 5) Effects of vanadate on the spontaneous activities were quite different from those of ouabain; $10^{-6}M$ vanadate increased the amplitude of contractions and $10^{-5}M$ vanadate increased slightly both amplitude and frequency of spontaneous contractions. $10^{-4}M$ vanadate showed irregular phasic contractions superimposed on the increased basal tone. 6) $10^{-5}M$ vanadate depolarized the membrane potential and shortened the interval between the bursts of spike discharge, whereas $10^{-4}M$ vanadate induced continuous spike discharge with membrane depolarization. 7) Vanadate caused a characteristic inhibitory response to the contractions induced by electrical stimulation; An initial rapid inhibition of tension development and then gradual recovery to a certain level. From the above results, the following conclusions could be made: 1) The rise of basal tone at $10^{-7}M$ ouabain is due to continuous spike discharge without a silent period. The continuous spike discharge is likely to be associated with a slight membrane depolarization caused by the blockage of Na pump. 2) The biphasic response induced by above $10^{-6}M$ ouabain seems to occur by the different mechanisms. The initial increase in tension is associated with depolarization along with an increase in spike frquency, whereas the subsequent relaxation occurs through a non-electrical mechanism. 3) The characteristic response to $10^{-7}M$ ouabain is resulted not from the action on intrinsic nerve terminal, but from its direct action on the membrane of smooth muscle cells. 4) The phasic contractions superimposed on the increased basal tone at the concentration of $10^{-4}M$ vanadate is resulted from the continuous spike discharge with membrane depolarization, of which mechanism remains unknown. 5) The inhibitory action of ouabain on the electrically induced contractions suggests that the increasein intracellular Na in some way inhibits the electrically induced $Ca^{2+}$ influx. The mechanism of vanadate action on the induced contractions remains unknown.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1998년도 학술발표회
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• pp.29-29
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• 1998

Previously, we reported two types of vanadate-sensitive ATPases in the micro somes of tracheal epithelial cells, a high-affinity one and a low-affinity one. The low affinity vanadate-sensitive (LAVS) ATPase was sensitive to thapsigargin and cyclopiazonic acid, specific antagonists of ER-type Ca$\^$2＋/-ATPase, and mediated microsomal $\^$45/Ca$\^$2+/ uptake, implying that the LAVS-ATPase is an ER/SR-type Ca$\^$2+/-ATPase.(omitted)

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
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• pp.125-126
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• 2009

In this study, we report the room temperature synthesis and luminescence properties of white-light-emitting Rb(V,P)$O_3$. The vanadate phosphor, RbV$O_3$, was synthesized by simple mixing of $RbCO_3$ and $V_2O_5$ at room temperature in air. New direct room temperature solidstate reaction is a cost-effective method to synthesize the above luminescent materials.