• BMB Reports
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• 제41권2호
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• pp.158-163
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• 2008

Hepatitis B virus X protein (HBx) is essential for hepatitis B virus infection and exerts a pleiotropic effect on various cellular machineries. HBx has been also demonstrated as an indirect transcriptional transactivator of various different viral and cellular promoters. In addition, HBx is involved in the development of various liver diseases including hepatocellular carcinoma. However the mechanism of HBx in hepatocellular carcinogenesis remains largely unknown. In this study, to identify possible new cellular proteins interacting with HBx, we carried out yeast two-hybrid assay. We obtained several possible cellular partners including VBP1, a binding factor for VHL tumor suppressor protein. The direct physical interaction between HBx and VBP1 in vitro and in vivo was confirmed by immunoprecipitation assay. In addition, we found that VBP1 facilitates HBx-induced $NF{\kappa}B$ activation and cell proliferation. These results implicate the important role of HBx in the development of hepatocellular carcinoma through its interaction with VBP1.

• 대한수의학회지
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• 제41권1호
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• pp.37-42
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• 2001

This research was conducted to investigate the effects of far infrared radiological materials on growth performance, immune response and changes of microbial flora in feces for growing pigs. Twelve growing pigs($Duroc{\times}Yorkshire{\times}Landrace$, average initial body weight of $15.6{\pm}0.5kg$) were used in a 40 day growth assay. Treatments were control(saline injection with diet: S); saline injection with Bio-Plus in diet(SBP); and vaccination with Bio-Plus in diet(VBP) in a randomized complete block design with initial BW as the blocking criterion. Serum concentrations of IgG of SBP and VBP were higher than those of S at day 10(p<0.05), 20(p<0.05), 30(p<0.05) and 40(p<0.05). Pigs fed with treatment diets had increased lymphocyte level compared to S at day 20(p<0.05) and 40(p<0.05). Cortisol was lower in treatments than in S at day 30(p<0.05). At day 20, there was no significant difference in E. coli among the treatments. However, it was observed that E, coli of the treatments was decreased compared to S at day 40(p<0.01). Lactobacillus of SBP was significantly higher(p<0.05) than that of S at day 40. In conclusion, the results of the experiment suggest that far infrared radiological materials could be a very beneficial immune response for growing pigs in health aspects.

• Geomechanics and Engineering
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• 재33권6호
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• pp.553-565
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• 2023

As the structure of broken rock mass is complex, with obvious discontinuity and anisotropy, it is generally necessary to reinforce broken rock mass using grouting in underground construction. The purpose of this study is to experimentally investigate the mechanical properties of broken rock mass after grouting reinforcement with consideration of the characteristics of broken rock mass (i.e., degree of fragmentation and shape) and a range of reinforcement methods such as relative strength ratio between the broken rock mass and cement-based grout stone body (λ), and volumetric block proportion (VBP) representing the volumetric ratio of broken rock mass and the overall cement grout-broken rock mass mixture after the reinforcement. The experimental results show that the strength and deformation of the reinforced broken rock mass is largely determined by relative strength ratio (λ) and VBP. In addition, the enhancement in compressive strength by grouting is more obvious for broken rock mass with spherical shape under a relatively high strength ratio (e.g., λ=2.0), whereas the shape of rock mass has little influence when the strength ratio is low (e.g., λ=0.1). Importantly, the results indicate that columnar splitting failure and inclined shear failure are two typical failure modes of broken rock mass with grouting reinforcement.

• Radiation Oncology Journal
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• 제13권2호
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• pp.181-189
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• 1995

목적: 국소적으로 진행된 자궁경부암의 치료는 방사선 단독요법이 주 치료였으나 근래에는 좀 더 완치율을 높이기위해 약물치료와 병행하여 방사선치료가 시행되고 있다. 저자들은 45명의 방사선 단독요법과 77명의 항암화학과 방사선의 병용요법의 치료성적을 후향적으로 분석하였다. 방법: 1985년 1월부터 1991년 12원까지 한양대학병원 치료방사선과에서 외부방사선 및 강내방사선치료를 받은 환자 122명을 대상으로 후향적으로 분석하였다. 34명은 ICR 1회, 77명은 ICR 2회, 11명은 고선량 강내근접 방사선치료를 받았다. 연령분포는 29세부터 81세였고, 중앙연령은 58세였다. 추적기간은 5개월에서 117개월(5명이 34개원 미만)로 중앙값은 60개월이었다. 조직병리상 111명이 squamous cell ca, 5명이 large cell ca, 4명이 adenoca, 2명이 adenosquamous cell ca였다. FIGO 병기로 IB $6명(4.9\%),$ IIA 11명$(9.0\%),$ IIB 37명$(30.3\%),$ IIIA 3명$(2.5\%),$ IIIB 63명$(51.6\%),$ IVA 2명$(1.6\%)$이었다. 항암화학요법을 병용한 환자 77명중 36명은 VBP, 39명은 cispiatin 과 5-FU, 2명은 5-FU 제제로 치료받았다. 결과: 외부방사선조사량 3900 cGy에서 5500 cGy를 받은환자에서 61명$(50\%)$이 육안적으로 완전관해를보였다. 5년 및 9년 생존율은 전체환자에서 각각 $57.8\%,\;53.9\%$였고 방사선 단독요법의 5년 생존율이 $63.1\%,$ 방사선과 항암화학복합요법의 5년 생존율은 $55.9\%$였다. 항암제 VBP(vinblastin, bleomycin, cisplatinum)로 치료받은 환자에서 5년 생존율이 $63\%$이고 cisplatin으로 치료받은 환자는 $50\%$이나 통계적인 유의성은 없었다. ICR 회수에따른 5년 생존율은 1회 ICR에서 $36.5\%,$ 2회 ICR에서 $67\%$였다. 방사선단독요법으로 치료받은 환자중 A point에 8000 cGy이상 받은 환자에서 5년 생존율이 $91.4\%$이었고 8000 cGy 미만 환자는 $27.2\%$로 통계적으로 유의한 생존율의 향상을 보였다(p<0.01). 항암화학요법으로 치료받은 환자는 A point의 dose에 따른 생존율의 차이를 보dl지 않았으며(<8000 cGy $55.6\%\;vs\;<=8000\;cGy\;55.7\%)$ 1회의 ICR 과 2회의 ICR의 5년 생존율에도 큰 차이가 없었다$(50\%\;vs\;56.8\%).$ 병변크기가 3cm 이상인 환자에서 방사선 단독치료법과 항암화학치료법과의 5년 생존율의 차이가 없었다$(48.9\%\;vs\;48.7\%).$ 초기 병변에서 항암화학치료를 받지않은 군과 받은 관의 5년 생존율은 $60\%$와 $78\%$이나 숫자가 매우적어 의미가 없었고 진행된병기에서 5년 생존율은 방사선 단독군이 조금 좋았으나 통계적 유의성은 없었다$(62.6\%\;vs\;53.6\%)$ (p>0.05). 결론: 본 연구결과 방사선치료에 항암화학 병용은 생존율향상을 시키지 못하였다. 방사선단 독요법이 국소적으로 진행된 환자에 치료방법이며 A point에 8000 cGy이상 받은환자에서 생존율이 높았고 항암화학 병용요법 치료에서는 A point의 방사선양이 생존율에 영향을 미치지 않았다.