• Journal of Preventive Medicine and Public Health
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• v.29 no.1 s.52
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• pp.43-50
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• 1996

We experienced a case of nephropathy in chronic lead poisoning. The patient was 43-year-old male who has been working in secondary lead smelting plant for 14 years. On admission, blood pressure was 160/90 mmHg and the others were non-specific. In past history, he received chelating agent administration for lead poisoning irregularly and medicated for gout, and the blood lead concentration was $180.0{\mu}g/dl$ on 2 months before admission. Smoking habit has been 1 pack per day for 15 years and drinking habit has been 1 bottle of Soju per day but less flow. In liver function test, AST/ALT were 27/28 IU/l and $\gamma-GT$ was 456 IU/l. In blood test, Hb : 11.5 g/dl, Hct : 34.0% and basophilic stipplings were found in peripheral blood smear. Chest PA was normal and abdominal ultrasonographic finding was non-specific except fatty liver. In the test of lead exposure indices, $PbB:83.0{\mu}g/dl,\;PbU:28.3{\mu}g/l$, and blood ZPP was $300.0{\mu}g/dl$. And in renal function test, BUN : 31.4 mg/dl, blood creatinine : 2.7mg/dl, blood uric acid. 9.1 mg/dl, urinary albumin : 100.0 mg/g creatinine, urinary $\alpha_1-microglobulin$ : 120.5 mg/g creatinine, urinary $\beta_2-microglobulin$ : $183.8{\mu}g/g$ creatinine, and 24 hours urinary creatinine clearance was 31.9 ml/min. The ultrasonoguided renal biopsy showed the global sclerosis of glomerulus, moderate atrophy and loss of tubule, and interstitial fibrosis in light microscopy. There were diffuse losses of brush border of proximal tubule in electronmicroscopy.

• Journal of dental hygiene science
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• v.15 no.1
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• pp.32-37
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• 2015

The purpose of this study was to quantify urinary and salivary bisphenol-A (BPA) concentrations according to sealant procedure among children. Nine students who had never treated with composite resin or sealant before, were recruited from one elementary school, Daegu, Korea from August 2013 to April 2014. Before sealant procedure, saliva and urine sample were collected. Immediately after sealant procedure saliva sample was collected and 24 hours after the procedure urine sample was collected. Creatinine was measured and adjusted to calculate urinary BPA concentration. Salivary and urinary BPA concentration after sealant procedure were $2.43{\pm}1.54{\mu}g/L$, $4.08{\pm}3.05{\mu}g/g{\cdot}$creatinine respectively, which were relatively higher than those before sealant procedure ($1.41{\pm}1.06{\mu}g/L$, $2.89{\pm}2.91{\mu}g/g{\cdot}$creatinine) but these were not statistically significant. We suggest that more large scale studies considering environmental confounders which have an effect on BPA are needed to establish the relationship between sealant exposure and BPA among children.

• Journal of Nutrition and Health
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• v.20 no.2
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• pp.90-103
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• 1987

A study was conducted to measure nitrogen intake and excretion in young korean women on their normal diet and leading normal activity level. Dietary survey by food record, fecal and urinary samples were collected for 3 days in 19 healthy female college students in Korea. On one of the 3 days, duplicate of one-day diet was collected from each subjects. Mean daily intakes of energy and protein were calculated from food recorded. Duplicate diet samples and pooled fecal samples were analyzed for N content. Pooled urine samples were analyzed for total N, urea N, and creatinine content. Apparent N absorption, apparent N balance and urea N/creatinine N were calculated to evaluate protein nutritional status. The results obtained are summarized as following ; 1) Accordingly to food record, mean daily intakes of energy, protein, carbohydrate and far were 1535.2 $\pm$53.78Kcal, 55.95$\pm$2.97g(total nitrogen 8.95$\pm$0.45g), 254.13$\pm$10.31g and 39.24$\pm$2.76 g, providing 14.6%, 66.2%, 19.2% of total energy respectively. 2) Nitrogen intake by chemical analysis was 7.16$\pm$0.31g/day (protein 44.75$\pm$1.94kg/day) providing 82.39$\pm$4.58% of nitrogen intake by food record. The difference of total nitrogen intake between food record and chemical analysis in diets was significant(p<0.05). 3) Mean daily fecal nitrogen excretion was 1.38$\pm$0.10g and then mean apparent digesbility of protein was 80.53$\pm$5.21%. 4) Mean daily urinary nitrogen excretion, urea N excretion and creatinine excretion were 6.03$\pm$0.30g, 4.52$\pm$0.22g, and 0.88$\pm$0.04g respectively. Urinary urea nitrogen was 75.2$\pm$1.38% of total urinary nitrogen excretion and urinary nitrogen was 85.4$\pm$3.56% of total nitrogen intake by chemical analysis. 5) Mean urea N/creatinine N ration was 14.01$\pm$0.77. 6) Mean nitrogen balance was -0.244$\pm$0.33g/day. From the above results, it is concluded that the subjects in this study seem to be in marginal protein nutritional status and therefore should increase dietary protein intake.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.4
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• pp.594-603
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• 1994

This study investigate the balance and biochemical status of riboflavin in Korean men. During the experimental period, four riboflavin diets with different levels of riboflavin(0.4, 0.6,0.8 and 1.0mg/1000kcal) were followed by eight healthy college men. the riboflavin status was assayed by erythrocyte glutathine reudcctase activity coefficient (EGRAC0 and urinary excretion of riboflavin. Riboflavin intake of the subjects who consumed a det was 0.46mg/1000kcal. the riboflavin intakes of the subjects who consumed the experimental diet with 0.4, 0.6, 0.8 and 1,0mg/1000kcal of riboflavin were 0.41, 0.60, 0.81 , 0.97mg, respectively. Fecal riboflavin loss, absorbed riboflavin , urinary riboflavin loss and retained riboflavin increased in the subjects consumed 0.4, 0.6, 0.8 and 1.0mg/1000kcal of riboflavin. The average EGRAC values for the subjects consumed 0.4, 0.6, 0.8 and 1.0mg/1000kcal of riboflavin were 1.303$\pm$0.029, 1.271$\pm$0.022, 1.239$\pm$0.013, 1.202$\pm$0.030, respectively and urinary riboflavin values ($\mu\textrm{g}$/g creatinine) were 86.89$\pm$ 20.07, 123.88$\pm$ 15.88, 240.70$\pm$57.14 and 393.36$\pm$76.94, respectively. Results indicate that 0.6mg/1000kcal is the level of riboflavin intake needed to maintain urinary riboflavin within the normal range. And above1.0mg/1000kcal of riboflavin is need to maintain urinary riboflavin with in the normal range. And above 1.0mg/1000 of riboflavin is needed to maintain the EGRAC within the normal range. The riboflavin intake correlated positively with urinary riboflavin value, but correlated negatively with the EGRAC value. The EGRAC value correlated negatively to protein intake as well as animal protein intake. The linear equation of between riboflavin intake and EGRAc was EGRA=-0.1667 $\times$riboflavin intake +1.3710. The riboflavin intake to maintain EGRAc below 1.20 was calculated 1.02mg/1000kcal by the above equation.

• Journal of Preventive Medicine and Public Health
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• v.43 no.4
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• pp.301-308
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• 2010

Objectives: In most DEHP exposure assessment studies, single spot urine sample was used. It could not compare the exposure level among studies. Therefore, we are going to represent the necessity of selection of proper sampling time of spot urine for assessing the environmental DEHP exposure, and the association urinary DEHP metabolites with steroid hormones. Methods: We collected urine and plasma from 25 men. The urine sampling times were at the end of the shift (post-shift) and the next morning before the beginning of the shift (pre-shift). Three metabolites of DEHP {mono(2-ethylhexyl) phthalate [MEHP], mono-(2-ethyl-5-hydroxyhexyl)phthalate [MEHHP], and mono(2-ethyl-5-oxohexyl)phthalate [MEOHP]} in urine were analyzed by HPLC/MS/MS. Plasma luteinzing hormone, follicle stimulating hormone, testosterone, and $17{\beta}$- estradiol were measured at pre-shift using a ELISA kit. A log-transformed creatinine-adjusted urinary MEHP, MEHHP, and MEOHP concentration were compared between the post- and pre-shift. The Pearson’s correlation was calculated to assess the relationships between log-transformed urinary MEHP concentrations in pre-shift urine and hormone levels. Results: The three urinary metabolite concentrations at post-shift were significantly higher than the concentrations in the pre-shift (p<0.0001). The plasma hormones were not significantly correlated with log-transformed creatinine - adjusted DEHP metabolites. Conclusions: To assess the environmental DEHP exposure, it is necessary to select the urine sampling time according to the study object. There were no correlation between the concentration of urinary DEHP metabolites and serum hormone levels.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.12 no.2
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• pp.53-66
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• 1999

This study has been carried out to investigate the effects of Dojuksan on the renal functions and internal secretion system, as water balance, urine volume, urinary excretion of sodium and potassium, free water clearance, urinary excretion of creatinine, plasma levels of artrial natriuretic peptide (ANP), plasma levels of aldosterone and plasma renin activity, comparing experimental group which Dojuksan water extract were administrated with control group. Sprague-Dawley rats, about 200-250 g, were used for this experiment. The results of this study were as follows: 1. Water balance decreased significantly after the administration of Dojuksan water extract. 2. Urine volume increased significantly after the administration of $100{\mu}l$ Dojuksan water extract per 100g rat. 3. Urinary excretion of sodium increased significantly but urinary excretion of potassium did not change after the administration of Dojuksan water extract. 4. Free water clearance decreased significantly after the administration of Dojuksan water extract 5. Urinary excretion of creatinine increased significantly after the administration of Dojuksan water extract 6. Plasma renin activity did not change after the administration of Dojuksan water extract 7. Plasma levels of artrial natriuretic peptide (ANP) did not change after the administration of Dojuksan water extract 8. Plasma levels of aldosterone decreased significantly after the administration of 200 ${\mu}Dojuksan water extract per l00g rat The results suggest that Dojuksan increase the urinary excretion of sodium. and thus reduce the water balance, which resulted from suppression of sodium reabsorption into renal tubule by increasing glomerular filtration rate and decreasing aldosterone.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.24 no.1
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• pp.52-58
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• 2014

Objectives: Using laborers participating in the petrochemical factory turnaround process as subjects, this study aims to identify exposure to benzene in the air and examine the relationship between exposure and the excretion of urinary metabolites by measuring concentrations of urinary trans, trans-muconic acid (t,t-MA). Methods: A passive sampler was used to measure the level of benzene in the air. In order to analyze urinary metabolites, the urine of laborers participating in the turnaround process was collected twice daily, both before and after work. In addition, a survey was conducted on work factors and lifestyle habits as factors affecting the concentration of urinary metabolites. Results: During the survey period, benzene was detected in the samples from all workers, and its average concentration was $0.16{\pm}0.22ppm$. The average concentration of t,t-MA after work was $1.20{\pm}1.86mg/g$ creatinine, and the results of analyzing urinary metabolites concentration before and after work showed statistically significant differences(p=0.003). There was also a statistically significant correlation (r=0.52, p=0.002) between benzene in the air and the concentration of after-work urinary t,t-MA. Conclusions: During the turnaround process, the average benzene concentration in workers was $0.16{\pm}0.22ppm$, which was below the exposure limit. However, their average t,t-MA concentration was $1.20{\pm}1.86mg/g$ creatinine, which exceeded the exposure limit of 1mg/g creatinine. The characteristics of turnaround process work require considerations such as underestimating the passive sampler being used and the skin absorption of benzene, and there needs to be a simultaneous assessment of working environment measurements and biological monitoring.

• Journal of Nutrition and Health
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• v.16 no.3
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• pp.209-215
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• 1983

In high sodium societies, the incidence in blood pressure with childhood growth is more abrupt than the rate of rise in low sodium populations. Thus, it appears that a lower level of dietary sodium intake is required to correct established hypertension and to prevent its appearance In present work, an investigation was made to estimate the correlation between urinary sodium, potassium and creatinine excretion, weight, height, upper arm circumference, blood pressures and the number of heart rate. Sixty- four children aged 12-16 years (41 boys and 23 girls) were measured. Twenty -four-hour urinary sodium and potassium excretion averaged 132.8 mEq and 42.1 mEq in boys, 126.4 mEq and 41.3 mEq in girls. Twenty- four -hour urinary creatinine excretion averaged 795.7 mg and 744.3mg in boys and girls, respectively. Systolic and diastolic blood pressure were 117.6mmHg and 49.7mmHg in boys, 95.5mmHg and 58.2mmHg in girls. Systolic blood pressure correlated positively weight, height and urinary creatinine but diastolic blood pressure correlated positively with upper arm circumference and negatively with urinary potassium. It was concluded that urinary sodium does not explain the blood pressure.

• Childhood Kidney Diseases
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• v.3 no.1
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• pp.27-34
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• 1999

Purpose: This study was designed to investigate the changes in soluble interleukin-2 receptor (sIL-2R) level in sera and urines of children with primary nephrotic syndrome, eliminating the confounding effects of age, proteinuria, and steroid treatment. Methods: Soluble IL-2R was measured by ELISA in sera and urines from patients with minimal change nephrotic syndrome or focal segmental glomerulosclerosis as well as from healthy controls. The serum levels and urinary sIL-2R/creatinine ratios were compared between control group and the 12 patient groups divided by their ages (0-1, 2-4, over 5 years), and presence or absence of proteinuria and/or steroid treatment (PU+Tx-, PU+Tx+, PU-Tx+, PU-Tx-). Results: Though the differences were not statistically significant probably because of the small numbers, serum sIL-2R levels seemed to be higher in younger age groups both in patients and control group. Nephrotic children did not show higher serum levels than normal children. Among the patients, proteinuric condition seemed to raise and steroid treatment tended to suppress the serum sIL-2R levels. Urinary sIL-2R/creatinine ratios were higher in younger age groups, more significantly in patients (P<0.001). Proteinuria and steroid treatment affected the urinary sIL-2R/creatinine ratios by the same way as the serum sIL-2R levels. Serum sIL-2R levels and urinary sIL-2R/creatinine ratios were not different between groups of different histologic findings or steroid responsiveness (P>0.05). Conclusion: Serum sIL-2R levels and the urinary sIL-2R/creatinine ratios were higher in younger age, and they were not higher in nephrotic patients compared to control group. The patients in relapse showed higher levels, while the levels were suppressed with steroid treatment. In proteinuric state, urinary sIL-2R/creatinine ratios reflected serum sIL-2R levels.

• Food Quality and Culture
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• v.2 no.2
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• pp.89-96
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• 2008

In order to determine whether green tea tannin ameliorates abnormal arginine metabolism as the result of excessive arginine, we have assessed the effects of the administration of green tea tannin mixture in rats treated 30 days with 2% arginine. In the arginine-treated group, the level of guanidino compounds such as arginine (Arg), guanidinoacetic acid (GAA), creatinine (Cr), methylguanidine (MG) and guanidinosuccinic acid (GSA), nitric oxide, urea, protein and glucose increased significantly in the serum, urine and kidney, whereas the oxygen species-scavenging enzymes of kidney were reduced as compared with the non-arginine-treated group. By way of contrast, the administration of green tea tannin reduced blood urea nitrogen and serum creatinine, and reduced the urinary excretion of guanidinoacetic acid, creatinine, and $NO_2^-+NO_3^-$. The increased levels of urinary urea, protein and glucose in the arginine-treated group were also lowered by the administration of green tea tannin. In these groups, the activities of superoxide dismutase and catalase in the kidney were increased, thereby suggesting the involvement of radicals in the normalizing of kidney function. These results show that the abnormal renal function induced by the adminstration of excessive arginine in rats may be restored by treatment with green tea tannin.