• 제목/요약/키워드: Uptake rate

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Effects of Vanadate on Activity of $Ca^{++}$Activated ATPase and $Ca^{++}$ Uptake of Sarcoplasmic Reticulum in Rat Skeletal Muscle (Vanadate가 골격근 sarcoplasmic reticulum의 $Ca^{++}-ATPase$$Ca^{++}\;uptake$에 미치는 영향)

  • Joo, Soon-Jae;Han, Kyung-Hee;Leem, Joong-Woo;Kang, Doo-Hee
    • The Korean Journal of Physiology
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    • v.20 no.2
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    • pp.157-164
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    • 1986
  • Since it has been reported that vanadate inhibits $Ca^{++}-ATPase$ activity without affecting $Ca^{++}$ uptake, this study was undertaken to investigate the effects of vanadate on $Ca^{++}-ATPase$ activity and $Ca^{++}$ uptake in the sarcoplasmic reticulum of rat skeletal muscle. The following results were obtained. 1) $Ca^{++}$ activated ATPase activity of the intact sarcoplasmic reticulum was significantly inhibited when vanadate was added to the incubation medium at concentration greater than $10^{-6}\;M$. However $Mg^{++}$-ATPase activity of the intact SR was not affected by vanadate at concentrations ranging from $10^{-7}\;to\;10^{-4}\;M.$ Similarly, $Ca^{++}-ATPase$ activity in sonicated sarcoplasmic reticulum was significantly reduced by vanadate at a concentration $10^{-7}$ M or higher. 2) The uptake of $Ca^{++}$ by isolated sarcoplasmic reticulum was also inhibited by vanadate under the conditions where the turnover rate of $Ca^{++}-ATPase$ was made to increase. These results suggest that the inhibition of $Ca^{++}$ uptake by vanadate may be correlated with that of $Ca^{++}-ATPase$ if experimental conditions are properly set.

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Variations in Nutrients & CO2 Uptake Rates and Photosynthetic Characteristics of Saccharina japonica from the South Coast of Korea (다시마(Saccharina japonica)의 생장에 따른 영양염 및 CO2 흡수율과 광합성 특성 변화)

  • Hwang, Jae-Ran;Shim, Jeong-Hee;Kim, Jeong-Bae;Kim, Sook-Yang;Lee, Yong-Hwa
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.16 no.4
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    • pp.196-205
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    • 2011
  • To investigate the contribution of macroalgae to biogeochemical nutrients and carbon cycles, we measured the uptake rates of nutrients and $CO_2$ and characteristics of fluorescence of Saccharina japonica (Laminaria japonica Areschoug) using an incubation method in an acrylic chamber. From January to May 2011, S.japonica was sampled at Ilkwang, one of well-known macroalgae culture sites around Korea and ranged 46~288 cm long and 4.8~22.0 cm wide of whole thallus. The production rate of dissolved oxygen by S. japonica (n=25) was about $6.9{\pm}5.8{\mu}mol\;g^{-1}$ fresh weight(FW) $h^{-1}$. The uptake rate of total dissolved inorganic carbon ($TCO_2$), calculated by total alkalinity and pH, was $8.9{\pm}7.9{\mu}mol\;g^{-1}\;FW\;h^{-1}$. Mean nutrients uptake were $175.6{\pm}161.1\;nmol\;N\;g^{-1}\;FW\;h^{-1}$ and $12.7{\pm}10.1\;nmol\;P\;g^{-1}\;FW\;h^{-1}$. There were logarithmic relationships between thallus length and uptake rates of nutrients and $CO_2$, which suggested that younger specimens (<100-150 cm) were much more efficient at nutrients and $CO_2$ uptake than old specimens > 150 cm. There was a positive linear correlation ($r^2$=9.4) existed between the dissolved oxygen production rate and the $TCO_2$ uptake rate, suggesting that these two factors may serve as good indicators of S. japonica photosynthesis. There was also positive linear relationship between maximal quantum yield ($F_v/F_m$) and production/uptake rates of dissolved oxygen, $TCO_2$ and phosphate, suggested that $F_v/F_m$ could be used as a good indicator of photosynthetic ability and $TCO_2$ consumption of macroalgae. Maximum relative electron transport rate ($rETR_{max}$) of S. japonica increased as thallus grew and was high in distal part of thallus which may be resulted from the increase of photosynthetic cell density per area. The annual $TCO_2$ uptake by S. japonica in Gijang area was estimated about $1.0\sim1.7{\times}10^3C$ ton, which was about 0.02-0.03% of carbon dioxide emission in Busan City. Thus, more research should be focused on macroalgae-based biogeochemical cycles to evaluate the roles and contributions of macroalgae to the global carbon cycle.

Combination Dyeing of Silk Fabrics with Extracts from Humulus japonicus and the Pruned Branches of Prunus persica Tree (환삼덩굴과 복숭아나무 전정가지 추출액을 이용한 견직물의 복합염색)

  • Ha, Young Kab;Lee, Jeong Sook
    • Textile Coloration and Finishing
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    • v.27 no.1
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    • pp.80-95
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    • 2015
  • In this study, the properties such as color, dye uptake and antibacterial function were examined through the application of combination dyeing and mixed dyeing with extracts from Humulus japonicus(hwansam) and the pruned branches of Prunus persica(peach) tree. In terms of dye uptake and surface color changes subject to the kind of mordants, methods and mixing ratio of dye, it was found that fabrics dyed by the pre-mordanting method with $CuSO_4$ showed the red green yellow group colors, which were not seen in fabrics dyed with hwansam only. Color fastness was found strong at grade 4-5 with 96.3% of UV protection ratio. While the fastness to light was as fair as grade 3, it showed an excellent result of 99.9% bacteria reduction rate against Staphylococcus aureus with fair 58% deodorization rate and 21.2% antibacterial rate against Klebsiella pneumoniae. As stated above, the combination and mixed dyeing resulted in the meaningful outcomes with regard to color variations, dye uptake, color fastness and functional properties.

Tentative Classification of Milled Rice by Sorption Kinetics (수화 특성에 의한 쌀의 분류)

  • Kim, Sung-Kon;Jeong, Soon-Ja;Kim, Kwan;Chae, Jae-Chun;Lee, Jung-Haeng
    • Applied Biological Chemistry
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    • v.27 no.3
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    • pp.204-210
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    • 1984
  • Hydration of twelve japonica (j) and nine j x indica rice varieties was analyzed in terms of mathematical rate equation and a tentative classification of milled rice was attempted primarily on the basis of water uptake race of rice grain at room temperature. No two rice varieties had the same water uptake rate or diffusion coefficient. The rice samples could be classified into three groups. Rices which are considered as having good eating quality had lower water uptake rate.

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A Study on Moisture Related Properties and Human Sensations of Underwear (1) -A Study on Water and Water Vapor Transport characteristics of Underwear Fabrics- (시판 내의류소재의 수분특성 및 착용감에 관한 연구 (I) -시판 내의류 소재의 수분특성-)

  • 이순원
    • Journal of the Korean Home Economics Association
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    • v.26 no.4
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    • pp.1-13
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    • 1988
  • The purpose of this study is to investigate water and after vapor transport characteristics of underwear fabrics. Experimental materials were cotton woven fabric and cotton knitted fabric, nylon tricot (untreated and hydrophilic finished) and cotton/polyester/cotton triple layer. Cotton knitted fabric have three types of knit structure (interlock, rib, plain stitch) and knit with either 38's or 60's combed yarn. And cotton woven fabric have plain weave with 60's combed yarn. As experimental methods, vapor cup test, dynamic method, vertical wicking test and transplanar uptake test were used. The results are as follows. 1) In cotton specimens, the order of water vapor transpiration (wvt) was plain > rib > interlock in the same yarn diameter. The knit fabric of thinner yarn showed the better wvt among the same knit structure. 2) In cotton specimens, the order of water absorbency was interlock > rib > plain in the same yarn diameter. the knit fabric of thicker yarn showed the better absorbency among the same knit structure. 3) When knit fabric (60's plain) is compared with woven fabric 960's plain), knit fabric showed faster rate of wvt, more amount of uptake and slower rate of water uptake than woven fabric did. 4) When compared untreated nylon with hydrophilic finished nylon, hydrophilic finished nylon showed much more water absorbency than untreated nylon did, but showed same rate of wvt. 5) The water transport characteristics of triple layer underwear fabric showed that the thinner and the lighter one, the better wvt and absorbency did.

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The Effects of Beta-blocker and Aerobic Exercise on Heart Rate Recovery and Exercise Capacity after Maximal Exercise in Acute Coronary Syndrome Patients

  • Cha, Kyung-Hwa;Oh, Jae-Keun;Kim, Young-Joo
    • Biomedical Science Letters
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    • v.19 no.1
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    • pp.70-78
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    • 2013
  • This study was conducted to identify the effects of intake of beta-blocker and aerobic exercise on heart rate recovery (HRR) and exercise capacity after maximal exercise in acute coronary syndrome patients (ACS). Subjects were divided into 4 groups; Beta-blocker-Exercise group (n=10), Beta-blocker group (n=10), Exercise group (n=10), Control group (n=10). Symptom-limited grade exercise test were conducted on subjects pre- and post- 6 week study, to measure maximal heart rate, heart rate at 1 minute and 2 minutes at the recovery stage, maximal oxygen uptake and maximal exercise time. Study resulted in significant improvements in heart rate recovery for BB-Ex group ($17.40{\pm}5.58$ bpm to $26.10{\pm}6.66$ bpm), maximal oxygen uptake for BB-Ex group ($30.46{\pm}8.63$ to $36.33{\pm}8.10$) and Ex group ($29.04{\pm}4.93$ to $34.31{\pm}5.19$), and total exercise time for BB-Ex group ($906.30{\pm}158.57$ to $1018.50{\pm}151.21$), BB group ($805.70{\pm}182.94$ to $897.50{\pm}160.16$) and Ex group ($870.00{\pm}145.63$ to $961.90{\pm}107.29$). Therefore it showed the biggest improvement for heart rate recovery and exercise capacity in acute coronary syndrome patients when both Beta-blocker and aerobic exercise were in effect.

Inhibition of Sarcoplasmic Reticulum $Ca^{2+}$ Uptake by Pyruvate and Fatty Acid in H9c2 Cardiomyocytes: Implications for Diabetic Cardiomyopathy

  • Lee, Eun-Hee;Lee, Hye-Kyung;Kim, Hae-Won;Kim, Young-Hoon
    • The Korean Journal of Physiology and Pharmacology
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    • v.9 no.4
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    • pp.195-201
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    • 2005
  • High extracellular glucose concentration was reported to suppress intracellular $Ca^{2+}$ clearing through altered sarcoplasmic reticulum (SR) function. In the present study, we attempted to elucidate the effects of pyruvate and fatty acid on SR function and reveal the mechanistic link with glucose-induced SR dysfunction. For this purpose, SR $Ca^{2+}$-uptake rate was measured in digitonin-permeabilized H9c2 cardiomyocytes cultured in various conditions. Exposure of these cells to 5 mM pyruvate for 2 days induced a significant suppression of SR $Ca^{2+}$-uptake, which was comparable to the effects of high glucose. These effects were accompanied with decreased glucose utilization. However, pyruvate could not further suppress SR $Ca^{2+}$-uptake in cells cultured in high glucose condition. Enhanced entry of pyruvate into mitochondria by dichloroacetate, an activator of pyruvate dehydrogenase complex, also induced suppression of SR $Ca^{2+}$-uptake, indicating that mitochondrial uptake of pyruvate is required in the SR dysfunction induced by pyruvate or glucose. On the other hand, augmentation of fatty acid supply by adding 0.2 to 0.8 mM oleic acid resulted in a dose-dependent suppression of SR $Ca^{2+}$-uptake. However, these effects were attenuated in high glucose-cultured cells, with no significant changes by oleic acid concentrations lower than 0.4 mM. These results demonstrate that (1) increased pyruvate oxidation is the key mechanism in the SR dysfunction observed in high glucose-cultured cardiomyocytes; (2) exogenous fatty acid also suppresses SR $Ca^{2+}$-uptake, presumably through a mechanism shared by glucose.

Assessment of the Uptake of Base Cation and Nitrogen in Korean Forest (우리나라 산림에 의한 염기성 양이온과 질소의 흡수량 산정)

  • Lee, Sang-Deok;Han, Jin-Seok;Chung, Il-Rok;Lee, Sang-Yong
    • Journal of Environmental Impact Assessment
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    • v.18 no.1
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    • pp.41-50
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    • 2009
  • Using the "Statistical Yearbooks of Korean forestry", we assessed the uptake of nitrogen and base cations by Korean forest. Combined amount of base cations uptake by forest tress during its growth and that of at the time of harvest reached to 1,034 eq/ha/yr. The base cations uptake in the range of 900 ~ 1,100 eq/ha/yr occupied approximately 48.6% out of total. Coniferous forest in the range of 170 ~ 200 eq/ha/yr was 59.9%, deciduous forest in the range of 430 ~ 530 eq/ha/yr was 42.6%, and mixed forest in the range of 270 ~ 370 eq/ha/yr was 35.7% out of total. Deciduous forest recorded higher uptake rate of nitrogen and base cation than coniferous forest in Korea. Combined amounts of nitrogen uptake by forest tress during growth and that at the time of forest, was 1,108 eq/ha/yr and nitrogen uptake was in the range of 1,000 ~ 1,200 eq/ha/yr, Within Korea, forest with nitrogen uptake in the range of 1,000 ~ 1,200 eq/ha/yr account for 45.7% of the entire country. Locations in the range of 320 ~ 390 eq/ha/yr occupied 43.9% of all coniferous forest while deciduous forest in the range of 470 ~ 570 eq/ha/yr was 40.4% of total deciduous forest. As for mixed forest in the range of 270 ~ 370 eq/ha/yr, it occupied 35.9% of all mixed forest of Korea.

Hydrogen Absorption Behavior of Zr-2.5Nb Pressure Tubes in Wolsong Unit 1

  • Choo, Kee-Nam;Kwon, Sang-Chul;Kim, Young-Suk
    • Nuclear Engineering and Technology
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    • v.30 no.4
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    • pp.318-327
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    • 1998
  • The deuterium uptake behavior of Zr-2.5Nb pressure tubes in Wolsong Unit 1 was analyzed in terms of longitudinal location, operation time, and coolant temperature. The results were compared with those obtained from Canadian CANDU reactors. The amount of deuterium uptake was higher at the outlet part than at the inlet part and was also higher when subjected to a longer operation time and a higher coolant temperature. The hydrogen uptake of Zr-2.5Nb in a hydrogen gas atmosphere was dependent on the microstructure of the alloy. The aged Zr-2.5Nb consisting of $\alpha$-Zr and $\beta$-Nb phases showed higher hydrogen uptake than that consisting of $\alpha$-Zr and $\beta$-Zr phases. The hydrogen in the alloy decreased the rate of oxidation. This could be explained in terms of the cathodic controlled reaction of Zr-2.5Nb oxidation.

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Effects of Carbon Sources and Other Process Variables in Fed-Batch Fermentation of Penicillin (페니실린 발효 공정에 있어서 탄소원 및 다른 공정변수가 미치는 영향)

  • 이진선;신규철;양호석;유두영
    • Korean Journal of Microbiology
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    • v.16 no.1
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    • pp.21-29
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    • 1978
  • In the fed-batch fermentation of penicillin specific uptake rates of carbon source and ammonia nitrogen, and specific production rate of penicillin as the most important process variables were evaluated over the fermentation course and their effects on the productivity studied. As the results, glucose and lactose each as a major carbon source showed the following values, respectively ; the specific uptake rates of 47-93 mg hexose per gm-DCW per he and 37-44 mg hexose per gm-DCW per hr, the specific uptake rates of 4.6-6.8 mg $NH_3-N$ per gm-DCW per hr, and 1.2 mg $NH_3-N$ per gm-DCW per he and the specific production rates of 32-42 arbitrary unit per gm-DCW per hr and 46-50 arbitrary unit per gm-DCW per hr. The productivity of penicillin could be improved by controlling the feed rates of glucose and ammonia nitrogen to meet the uptake rates.

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