• ALGAE
• /
• v.19 no.1
• /
• pp.1-6
• /
• 2004

Carbon ($^{14}CO_2$) and nitrogen ($^{15}NH_4$ and $^{15}NO_3$) uptake were measured at two stations in the hypertrophic zone of the Seonakdong River, where Microcystis aeruginosa explosively bloomed in September 1998. Significant nitrogen limitation occurred in the period of Microcystis bloom, while phosphorus limitation was common in the river. The specific nitrogen ($NH_4$ + $NO_3$) uptake was 12-50 $\mu$mol mg chl-a$^{-1}$ hr$^{-1}$ at two stations, showing substantially higher than for any other freshwaters. The specific nirtogen uptake was higher at the GAR Station of the nitrogen-limited area and this high nirtogen uptake resulted in low $^{14}C:^{15}N$ atomic ratios of algal uptake. Carbon uptake was dependent upon irradiance, decreasing gradually toward the bottom in the euphotic zone, whereas the nitrogen uptake increased slightly toward the bottom. $NH_4$ preferable uptake against $NO_3$ was hardly discemilble due to the fact that it exceeded the $NH_4$ ambient concentraiton. The $^{14}C:^{15}N$ atomic ratios of algal uptake in the surface waters approached the Redfield C:N ratio.

• Korean Journal of Soil Science and Fertilizer
• /
• v.18 no.4
• /
• pp.413-418
• /
• 1985

Tobacco plant(8-leaf seedlings) were grown on water culture fertilized with different N sources ($NO_3-N$, $NH_4-N$, $NO_3+NH_4-N$) during 15 days. Daily uptake of nutrients and inorganic constituents in plants were investigated in relation to growth responses of them. 1. Nitrate-fed plant showed higher daily uptake of inorganic cations than those in other treatments, and reached about two times higher uptake of nitrogen and three times more uptake of cations (K, Ca, Mg). Potassium was preferentially uptaken at a very fast rate from the beginning after treatment. Also $NO_3-N$ tended to be taken up selectively by the plant from the mixture of nitrate and $NH_4-N$. 2. The initial pH (pH 6.0) of culture medium drastically changed into acid (pH 4.0) in the $NH_4-N$ medium, but into slightly higher (pH 6.4) in the nitrate when measured after exposure of 24 hours. The mixture also tended to show an acidity but much weaker than $NH_4-N$ solution. 3. Nitrate-fed plant had a normal growth pattern but $NH_4-N$ fed plant almost stopped growing. Those plants containing both nitrate and ammonium N were also showed very poor growth.

• Korean Journal of Soil Science and Fertilizer
• /
• v.10 no.4
• /
• pp.225-233
• /
• 1978

Uptake and distribution of labelled urea, $NH{_4}^+$, and $NO{_3}^-$ by Tongil and Jinheung rice grown with each nitrogen source until ear formation stage under water culture system were as follows. 1. When the previous nitrogen source was same as one tested the uptake rate ($mg^{15}N/g$ d.w. root 2hrs, at $28^{\circ}C$ light) was great in the order of $NH_4$ >urea> $NO_3$ and higher (especially $NH_4$) in Tongil than in Jinheung. Rate limiting step (slowest) seems to be exist at R (root)${\rightarrow}$LS(leaf sheath) for urea, LS${\rightarrow}$LB(leaf blade) for $NH_4$ and M(medium)${\rightarrow}$R for $NO_3$. The fast step of translocation appeare to be at M${\rightarrow}$R for urea R${\rightarrow}$LS for $NH_4$ and LS${\rightarrow}$LB for $NO_3$. 2. The uptake rate of $NH_4$ by the urea-fed plant increased almost linearly from $18^{\circ}C$ via $28^{\circ}C$ to $38^{\circ}C$ in Tongil ($Q_{10}$=1.21 and 1.32 respectively) while no change in Jinheung ($Q_{10}$=0.99 and 1.00 respectively). It decreased by 12% in Jinheung under dark but uo change in Tongil. 3. The uptake rate of nitrogen source by different source-fed plant was great in the order of $NH_4{\rightarrow}^{15}NO_3$ $NO_3{\rightarrow}^{15}NH_4$, $urea{\rightarrow}^{15}NO_3$ and higher (especially $NH_4{\rightarrow}^{15}NO_3$) in Tongil. In the case of $urea{\rightarrow}^{15}NH_4$ it was same in $NH_4{\rightarrow}^{15}NO_3$ for Tongil and slightly lower than that in $NO_3{\rightarrow}^{15}NH_4$ for Jinheung. It was lower (especially Tongil) in $NH_4{\rightarrow}^{15}NO_3$ than in $NH_4{\rightarrow}^{15}NH_4 $ 4. The uptake rate (in $NH_4{\rightarrow}^{15}NO_3$) was higher during 15 minutes than during 2 hours and always higher in Tongil. 5. $^{15}N$ excess % and content in each part, and uptake rate of root seems to have their own significance relatling with metabolism and translocation respectively. The change of nitrogen nutritional environment and source preference of varieties were discussed in relation to field condition and efficient use of nitrogen fertilizer.

• Korean Journal of Soil Science and Fertilizer
• /
• v.26 no.1
• /
• pp.31-36
• /
• 1993

A pot experiment was carried out to investigate the influence of application of nitrogen, phosphorus and calcium on the yield and uptake of Sr-90 by soybean plant. The results obtained were summarized as follows : 1. The application of N tended to decrease the dry matter yield of soybean. There was, however, difference in the effect on the yield between $NH_4-N$ and $NO_3-N$ ; the yield was higher in the former in the latter. On the other hand, the application of P and Ca increased the yield. 2. The uptake of Sr was decreased by the application of N, but such effect was greater in $NH_4-N$ than in $NO_3-N$. 3. The effect of N application in reducing the uptake of Sr. was enhanced by the application of P with N. 4. The combined application of P and lime showed significant effect of reducing the uptake of Sr by soybean plant. The uptake of Sr was lowest in the pot that received P, lime and $NH_4-N$ together.

• Applied Biological Chemistry
• /
• v.43 no.3
• /
• pp.163-168
• /
• 2000

To remove phosphate accumulated in the soil and water, Acinetobacter lwoffi PO8 possessing a high ability to accumulate phosphate was isolated from a active sludge. Bacterium was cultured in the liquid medium containing $150\;{\mu}g/mL$ of phosphate at $30^{\circ}C$ in different culture conditions to examine intracellular phosphate uptake. The initial pH in the range of $7.5{\sim}8.5$ was effective on the growth and phosphate uptake of the strain. Glycerol and arabinose used as a carbon sources showed 93 and 91% the phsphate uptake, respectively. Among the nitrogen sources, ammonium salt such as $NH_4NO_3$ and $(NH_4)_2SO_4$ was effectively utilized on the phosphate uptake compared with amino compounds. The rate of phosphate uptake of $NH_4NO_3$, and $(NH_4)_2SO_4$, was 95 and 96%, respectively The growth and Phosphate uptake ability in the strain were significantly promoted when metal ions were added in the medium; $Co^{2+}$, however, was not utilized by the strain. The capacity of phosphate uptake was enhanced to $10{\sim}20%$ when arginine, methionine, or lysine was added. Using $^{32}P$ to examine the uptake Pattern of intracellular phosphate, experiment result showed that polyphosphate was largely found in the fraction of intracellular inorganic phosphate of Acinetobacter lwoffi PO8.

• Korean Journal of Environmental Biology
• /
• v.17 no.2
• /
• pp.153-158
• /
• 1999

Uptakes of N and P by Phragmites japonica Steudel. are determined in continuous flow tanks as a function of hydraulic retention time (HRT, days) and nutrient concentration (NC). Results show that the uptake was higher at shorter HRT and higher nutrient concentration, and the regression equations were estimated. Mean above ground biomass of Phragmites japonica in the middle reaches of Sagimak stream was 335.92 g/$m^2$, and estimated uptake by this vegetation were 7.31~20.15 NH$_4$-N, 31.15~95.84 NO$_3$-N, and 4.09~11.48 PO$_4$-P mg/$m^2$/day in Sagimak stream, respectively when HRT was 1~5 days. According to this study, it is concluded that Phragmites japonica Steudel. are suitable for water quality improvement of stream through nutrient uptake at short HRT and high concentration.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.46 no.4
• /
• pp.303-308
• /
• 2001

To investigate the partitioning of newly absorbed N derived from NO$_3$- and NH$_4$$^{+}$, 6 mM $K^{15}$ NO$_3$ or 3 mM ($^{15}$ NH$_4$)$_2$ was fed continuously in Italian ryegrass (Lolium multiflrum L.) for 7 days. Nitrogen metabolites (nitrate, amino acid, soluble- and insoluble protein) were analyzed at the end of $^{15}$ N feeding. Dry weight in shoot, stubble and root was not significantly different between NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ feeding. Total nitrogen content in all three organs was significantly higher in NH$_4$$^{+}$ than NO$_3$$^{[-10]}$ feeding. Sum on N content in reduced N fractions (amino acids + proteins) in shoot, stubble and roots in NH$_4$$^{+}$ feeding increased by 13.3, 12.5 and 35.4 %, respectively, compared to NO$_3$$^{[-10]}$ feeding. The Relative Specific Activity (RSA, percentage of newly absorbed $^{15}$ N relative to total N in a sample) values of amino acids and insoluble proteins were significantly higher in NH$_4$$^{+}$ feeding. Total amount of newly absorbed $^{15}$ N in NO$_3$$^{[-10]}$ and NO$_3$$^{[-10]}$ feeding was 52.3 and 69.5 mg/plant on dry matter basis, respectively. In both NH$_4$$^{+}$ and NO$_3$$^{[-10]}$ grown plants, most of the N was allocated to the shoot, 67.5% in NH$_4$$^{+}$ feeding and 58.8% NO$_3$$^{[-10]}$ feeding, respectively. The $^{15}$ N amount incorporated in the reduced N compounds (amino acids and proteins) in NH$_4$$^{+}$ grown plants significantly increased by 74.8% compared to NO$_3$$^{[-10]}$ grown plants. The increase of the $^{15}$ N amount assimilated to amino acids in NH$_4$$^{+}$ grown plants was remarkably higher in roots as more than 7.25 times compared to NO$_3$$^{[-10]}$ feeding. These results indicated that Italian ryegrass was much efficiently utilized NH$_4$$^{+}$-N for the synthesis of reduced N compounds.reduced N compounds.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.58 no.3
• /
• pp.220-225
• /
• 2013

This experiment was carried out to elucidate the effects of silicate and different application forms of N on the uptake of water and N by rice plants. Three rice cultivars, Ilpum, Anda and M202, were grown under the hydroponics in a phytotron. One-hundred ppm silica was applied for silicate treatment. For nitrogen application forms were 100% $NH_4$ and $NH_4+NO_3$ in 2:1 ratio were applied. Silica treatment, compared to silica free, was very effective on the nitrogen uptake and dry weight increase of rice plants. Although silica application demonstrated no significant effect on the amount of water uptake, it improved increased water and nitrogen use efficiency. Therefore, sufficient application of silicate in paddy field will be useful for the growth of rice plants and water saving.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.42 no.5
• /
• pp.515-521
• /
• 1997

Tobacco plant was grown for 40 days hydroponically in nutrient solutions composed of different forms of nitrogen, like NO$_3$$^{[-10]}$ -N, NH$_4$$^{＋}$-N, and a mixed formulation of NO$_3$$^{[-10]}$ -N and NH$_4$$^{＋}$-N. Uptake response, nitrate reductase, and glutamine synthetase activity at growth stage were investigated to understand the basic knowledge of nitrogen metabolism. The better growth of shoot and root was observed in the mixed nutrient solution than NO$_3$$^{[-10]}$ -N or NH$_4$$^{＋}$-N, alone. The plant growth in NH$_4$$^{＋}$-N nutrient solution was poor due to ammonium toxicity. The pH of nutrient solution containing NO$_3$$^{[-10]}$ -N increased up to 40 days after transplanting. But the pH of solution containing NO$_3$$^{[-10]}$ -N decreased drastically to 3.42 at 20 days after transplant. The pH in the mixed formulation dropped to pH 3.64 at 30 days after transplant and showed re-increase. It is assumed that nitrogen of NH$_4$$^{＋}$-N form was taken up preferentially at early stage and NO$_3$$^{[-10]}$ -N form was taken up preferentially at middle stage in the treatment with the mixed solution. The result indicates that the relative proportion of nitrogen forms affected the uptake patterns at each growth stages. The contents of chlorophyll and soluble protein were high with the mixed solution. Total nitrogen content was the highest in NH$_4$$^{＋}$-N solution and the content also increased by the application of the mixed type of nitrogen. The amount of nitrate in leaves was high in NO$_3$$^{[-10]}$ -N treatment and the amount of ammonium was high in NH$_4$$^{＋}$-N treatment. The activity of nitrate reductase or glutamine synthetase was highest in the leaves grown in mixed nutrient solution than in those with any other single of nitrogen form.

• Journal of Bio-Environment Control
• /
• v.19 no.4
• /
• pp.229-233
• /
• 2010

This experiment was carried out to find optimum ratio of $NO_3^-$ to $NH_4^+$ in nutrient solution for the growth of Phalaenopsis hybrid and find the effect of the ratio of $NO_3^-$ to $NH_4^+$ on the flower stem (inflorescence) quality. The ratio of $NO_3^-$ to $NH_4^+$ of nutrient solution used in this experiment was 100% : 0%, 90% : 10%, 80% : 20% and 70% : 30%. Phalaenopsis showed better growth when $NH_4^+$ was supplied concurrently with $NO_3^-$ as nitrogen source than supplied with only $NO_3^-$. Especially, increasing the ratio of $NH_4^+$ from 0% to 10% the fresh weight and dry weight of Phalaenopsis hybrid was highest. But, the growth of shoot and root was diminished when the proportion of $NH_4^+$ in nutrient solution was increased from 10% to 30%. Inflorescence length, the number of inflorescence and flower per plant all increased as $NH_4^+$ increased from 0% to 10% but, decreased from 10% to 30%. These results suggest that the optimal ratio of $NO_3^-$ and $NH_4^+$ in nutrient solution for the growth of Phalaenopsis including inflorescence was founded to be 90%:10%.