• Title/Summary/Keyword: Up granule

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Histopathological and DNA Content Analysis of a Dermal Sarcoma in the Soft-shelled Turtle Pelodiscus sinensis

  • Syasina Iraida Germogenovna;Hur Jun-Wook;Kim Eun-Mi;Park In-Seok
    • Fisheries and Aquatic Sciences
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    • v.9 no.3
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    • pp.107-114
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    • 2006
  • A dermal sarcoma was found in a freshwater, soft-shelled turtle Pelodiscus sinensis. The neoplasm consisted of proliferating fibrous tissue and extended from the dermis. The overlying epidermis was hyperplastic and partially folded. The deeper dermis and hypodermis contained three large, discrete necrotic foci of -10 mm diameter. Numerous eosinophilic granule cells and macro phages surrounded the necrotic areas. A mixed population of cells with nuclear pleomorphism was observed between the papillary layers of vessels. This area also had regions of different histological structures: (l) regularly arranged, spindle-shaped cells with compact nuclei in a fine-fibrillar matrix; (2) haphazardly arranged cells ($\leq$ 23 11m diameter) with ovoid, highly hypertrophic, faintly stained nuclei; and (3) cells (3.6-5.8 11m diameter) with irregularly shaped nuclei and marginal condensed chromatin in a myxomatous matrix. Some mitotic figures, binucleate cells, and multinucleate giant cells of up to 50 11m in length were also found. Flow cytometry of propidium iodide-stained cells yielded different histograms for the normal skin and the skin (primarily epidermis) and fibrous dermis of the tumor, indicating DNA heterogeneity in the dermal portion of the tumor. The ploidy indices for the dermal cells were 1.91 and 0.78, as compared to normal cells.

A Study on Preparation and the Standard Recipe of Premixed Gam-Injulmi Rice Cake (Premix 감인절미 제조 및 표준 Recipe에 관한 연구)

  • 김경자;오옥자
    • Journal of the East Asian Society of Dietary Life
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    • v.7 no.1
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    • pp.47-55
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    • 1997
  • This study aims to know what the physicochemical properties and physical properties of the Korean traditional rice cake injulmi made from persimmon powder and glutinous rice(powder), and to come up with a standard recipe of it with the premixed powder of persimmon and glutinous rice. Cooked rice powder with 9 different levels of persimmon in cooling dough(75, 100 and 150${\mu}{\textrm}{m}$ power with each 20, 40, and 60%) was tested for rheological parameters, the rate of swelling, degree of gelatinization, sensory evaluation, and the observation of cross section used to electronmicroscope. 1. sensory evaluation conducted by 20 university students as panelists showed that mixed glutinous rice powder and persimmon powder lost their regular forms. increase in persimmon powder content increased a thin layer of starch granule, presumably due to sugar and fiber in the mixed persimmon powder granule. 3. In cooking the rice cake with 20~60% of persimmon, it showed 6.3 to 5.5 pH. 4. Quantitative description analysis conducted by 20 university student s panelists showed that sample that sample C was preferred among 9 samples. From these results, it was concluded that glutinous rice cake cooked with 20% of persimmon powder was quiet acceptable. Optimum cooking condition for the glutinous rice with persimmon powder rice cake was 30min of cooking time and 25$0^{\circ}C$ of cooking temperature with gas oven.

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Utilization of aerobic granulation to mitigate membrane fouling in MBRs

  • Iorhemen, Oliver T.;Hamza, Rania A.;Tay, Joo Hwa
    • Membrane and Water Treatment
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    • v.8 no.5
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    • pp.395-409
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    • 2017
  • Membrane bioreactor (MBR) is a compact and efficient wastewater treatment and reclamation technology; but, it is limited by membrane fouling. The control of membrane fouling significantly increases operational and maintenance costs. Bacteria and their byproducts - extracellular polymeric substances (EPS) - are major contributors to membrane fouling in MBRs. A recent attempt at fouling mitigation is the development of aerobic granular sludge membrane bioreactor (AGMBR) through the integration of a novel biotechnology - aerobic granulation - and MBR. This paper provides an overview on the development of AGMBR to mitigate membrane fouling caused by bacteria and EPS. In AGMBR, EPS are used up in granule formation; and, the rigid structure of granules provides a surface for bacteria to attach to rather than the membrane surface. Preliminary research on AGMBR using synthetic wastewater show remarkable membrane fouling reduction compared to conventional MBR, thus improved membrane filtration. Enhanced performance in AGMBR using actual municipal wastewater at pilot-scale has also been reported. Therefore, further research is needed to determine AGMBR optimal operational conditions to enhance granule stability in long-term operations and in full-scale applications.

Development of Up- and Down-flow Constructed Wetland for Advanced Wastewater Treatment in Rural Communities (소규모 오수발생지역의 고도처리시설을 위한 상.하 흐름형 인공습지 개발)

  • Kim, Hyung-Joong;Yoon, Chun-G.;Kwun, Tae-Young;Jung, Kwang-Wook
    • Journal of The Korean Society of Agricultural Engineers
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    • v.48 no.6
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    • pp.113-124
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    • 2006
  • The feasibility of the up- and down-flow constructed wetland was examined fur rural wastewater treatment in Korea. Many constructed wetland process was suffered from substrate clogging and high plant stresses because of long term operation. The up- and down-flow constructed wetland process used porous granule materials (charcoal pumice : SSR=10:20:70) for promoting intake rate of nutrient to plant, and especially flow type was designed continuously repeating from up-flow to down-flow. $BOD_5$ and SS was removed effectively by the process with the average removal rate being about 75% respectively. The wetland process was effective in treating nutrient as well as organic pollutant. Removal of TN and TP were more effective than other wetland system and mean effluent concentrations were approximately 7.5 and $0.4mg\;L^{-1}$ which satisfied the water quality standard for WWTPs. The treatment system did not experience any clogging or accumulations of pollutants and reduction of treatment efficiency during winter period because constructed polycarbonate glass structure prevented temperature drop. Considering stable performance and effective removal of pollutant in wastewater, low maintenance, and cost-effectiveness, the up- and down-flow constructed wetland was thought to be an effective and feasible alternative in rural area.

Confocal laser scanning microscopy image를 이용한 UASB granule의 메탄 생성 능력 측정

  • Lee, Yu-Jin;Kim, Hyo-Seop;An, Yeong-Hui;Park, Seong-Hun
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.365-369
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    • 2000
  • Methanogenic activity of granular sludge was monitored by specific methanogenic activity (SMA) assay and confocal laser scanning microscopy (CLSM) during start-up of a thermophilic UASB reactor. Autofluorescence by CLSM could visualize the methanogenic bacterial population inside sludge granules and its intensity was proportional to SMA. Considering the complex procedures of SMA measurement, fluorescence quantification by CLSM can be suggested as a routine technique measuring methanogenic activity in UASB granules.

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Enhancing mechanism of the saccharification of uncooked starch in an agitated bead reaction system (무증자전분의 분쇄마찰매체에 의한 효소당화촉진 Mechanism의 규명)

  • 조구형;이용현
    • Microbiology and Biotechnology Letters
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    • v.14 no.5
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    • pp.407-413
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    • 1986
  • In an agitated bead reaction system, the enzymatic saccharification of uncooked starch was substantially enhanced. The enhancement mechanism was investigated front the view of the structural aspect of starch. The mechanical impact caused by the movement of the attrition-milling media resulted neither the destruction of microcrystalline structure nor the fragmentation of starch granule. instead, the most distinct phenomenon was the swelling of starch granule up to about 2.5 times, and the swelling mechanism was not similar with that caused by cooking. However, in the case of the enzyme addition in the attrition coupled reaction system, the swollen starch was easily fragmented into the large number of small particles by the synergistic action of the enzyme and milling-media. The exposed surface area of the fragmented particles plays the major role in enhancing the saccharification. The saccharification rate was quite different depending on the source of starch, the reason was discussed in terms of the granular structure of uncooked starches.

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Observation of an Ellerman bomb and its associated surge with the 1.6 meter New Solar Telescope at Big Bear Solar Observatory

  • Yang, Heesu;Chae, Jongchul;Park, Hyungmin;Maurya, Ram Ajor;Cho, Kyuhyun;Kim, Yeon-Han;Cho, Il-Hyun;Lim, Eun-Kyung
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.111.2-111.2
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    • 2012
  • We observed an Ellerman bomb(EB) and its associated surge using the Fast Imaging Solar Spectrograph(FISS) and the broadband TiO filter of the 1.6 meter New Solar Telescope at Big Bear Solar Observatory. As is well-known, the EB appears as a feature that is very bright at the far wings of the H alpha line. The lambdameter method applied to these wings indicates that the EB is blue-shifted up to 6km/s in velocity. In the photospheric level below the EB, we see rapidly growing "granule-like" feature. The transverse velocity of the dark lane at the edge of the "granule" increased with time as reached a peak of 6km/s, at the time of the EB's occurrence. The surge was seen in absorption and varied rapidly both in the H alpha and the Ca II 8542 line. It originated from the Ellerman bomb, and was impulsively accelerated to 20km/s toward us(blueshift). Then the velocity of the surge gradually changed from blueshift of 20km/s to redshift of 40km/s. By adopting the cloud model, we estimated the temperature of the surge material at about 27000K and the non-thermal velocity at about 10km/s. Our results shed light on the conventional idea that an EB results from the magnetic reconnection of an emerging flux tube and pre-existing field line.

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Proteomic Analysis of Drought Stress-Responsive Proteins in Rice Endosperm Affecting Grain Quality

  • Mushtaq, Roohi;Katiyar, Sanjay;Bennett, John
    • Journal of Crop Science and Biotechnology
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    • v.11 no.4
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    • pp.227-232
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    • 2008
  • Drought stress is one of the major abiotic stresses in agriculture worldwide. We report here a proteomic approach to investigate the impact of post-fertilization drought on grain quality in rice seed endosperm (Oryza sativa cv. IR-64). Plants were stressed for 4 days at 3 days before heading. Total proteins of endosperm were extracted and separated by two-dimensional gel electrophoresis. Not many protein spots showed differential accumulation in drought-stressed samples. More than 400 protein spots were reproducibly detected, including three that were up-regulated and five down-regulated. Mass spectrometry analysis and database searching helped us to identify six spots representing different proteins. Functionally, the identified proteins were related to protein synthesis and carbohydrate metabolism, such as Granule-Bound Starch Synthase (GBSS, Wx protein), which is thought to play a very important role in starch biosynthesis and quality, a very crucial factor in determining rice grain quality.

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Alkaline Gelatinization of Starch during Steeping of Potato (감자의 수침에 따른 전분의 알칼리 호화 특성)

  • 김경애;김성곤;정난희;박영란
    • Korean journal of food and cookery science
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    • v.14 no.3
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    • pp.207-212
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    • 1998
  • The changes in physicochemical properties of potato were investigated while steeping in water for 7 days at 30${\pm}$1$^{\circ}C$. The shape of raw starch granules was round or oval, the starch granule showed birefringence distinctly under polarized light and it was kept clearly even after steeping. X-ray diffraction pattern of the starch was B-type and there was no change in the pattern after steeping. However, crystallinity was increased up to the 4th day and then decreased. Amylose contents of raw starch and the starch steeped for 7 days were 19.3% and 13.1%, respectively. When the potato starch was gelatinized in 0.15 N sodium hydroxide solution, the viscosity was decreased until the 3rd day, but increased thereafter. Gel volume of the starch in KSCN solution was decreased during steeping.

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Properties and Structure of High Frequency Soft Magnetic Nano-composite Films

  • Ohnuma, Shigehiro;Masumoto, Hiroshi
    • Journal of Magnetics
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    • v.16 no.4
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    • pp.403-407
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    • 2011
  • Metal-insulator type, nano-granular soft magnetic films have been reviewed from the viewpoint of high frequency magnetic materials. The formation of nano-granular structure is related to the magnitude of heat of formation of intergranule materials. Variation of the ratio of granule phase to intergranule phase in the film is found to produce various characteristics in the magnetic properties of the film. The HRTEM observation reveals that neighboring granules in the film with above 60 at.% Co, contact at considerable points and the films show soft magnetic properties which are explainable in terms of the random anisotropy model for nano-crystalline materials. Addition of Ni group elements in Co-O based films enhances their anisotropy field up to 400 Oe and they exhibit excellent frequency response of permeability. Also, large electromagnetic noise suppression effect is demonstrated as one of their potential applications.