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Study on CsRCI2D and CsRCI2H for improvement of abiotic stress tolerance in Camelina sativa L.

  • Lim, Hyun-Gyu;Kim, Hyun-Sung;Kim, Jung-Eun;Ahn, Sung-Ju
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.196-196
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    • 2017
  • Oilseed crop Camelina (Camelina sativa L.) is a suitable for biodiesel production that has high adaptability under low-nutrient condition like marginal land and requires low-input cost for cultivation. Enhanced abiotic stress tolerance of Camelina is very important for oil production under the wide range of different climate. CsRCI2s (Rare Cold Inducible 2) are related proteins in various abiotic stresses that predicted to localized at plasma membrane (PM) and endoplasmic reticulum (ER). These proteins are consist of eight-family that can be divided into tail (CsRCI2D/E/F/G) and no-tail (CsRCI2A/B/E/H) type of C-terminal. However, it is still less understood the function of C-terminal tail. In this study, CsRCI2D/H genes were cloned through gateway cloning system that used pCB302-3 as destination vector. And we used agrobacterium-mediated transformation system for generation of overexpression (OX) transformants. Overexpression of target gene was confirmed using RT-PCR and segregation ratio on selection media. We analyzed physiological response in media and soil under abiotic stresses using CsRCI2D and CsRCI2H overexpression plant. To compare abiotic stresses tolerance, wild type and CsRCI2D/H OX line seeds were sown on agar plate treated with various NaCl and mannitol concentration for 7 days. In the test of growth rate under abiotic stress on media, CsRCI2H OX line showed similar to NaCl and mannitol stress. In the other hand, CsRCI2D OX line showed to be improved stress tolerance that especially increased in 200mM NaCl but was similar on mannitol media. In greenhouse, WT and CsRCI2D/H OX lines for physiological analysis and productivity under abiotic stresses were treated 100, 150, 200mM NaCl. Then it was measured various parameters such as leaf width and length, plant height, total seed weight, flower number, seed number. CsRCI2H OX line in greenhouse did not show any changes in physiological parameters but CsRCI2D OX line was improved both physiological response and productivity under NaCl stress. Among physiological parameters of CsRCI2D OX line under NaCl stress, leaf length and width were observed shorter than WT but it were slightly longer than WT in 200mM NaCl stress. Furthermore, total seed weight of CsRCI2D OX line under stress displayed to decrease than WT in normal condition, but it was gradually raised with increasing NaCl stress then more than WT relatively. These results suggested CsRCI2D might be contribute to improve abiotic stress tolerance. However, function of CsRCI2H is need to more detail study. In conclusion, overexpression of CsRCI2s family can generate various environmental stress tolerance plant and may improve crop productivity for bio-energy production.

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Automatic Collection of Production Performance Data Based on Multi-Object Tracking Algorithms (다중 객체 추적 알고리즘을 이용한 가공품 흐름 정보 기반 생산 실적 데이터 자동 수집)

  • Lim, Hyuna;Oh, Seojeong;Son, Hyeongjun;Oh, Yosep
    • The Journal of Society for e-Business Studies
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    • v.27 no.2
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    • pp.205-218
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    • 2022
  • Recently, digital transformation in manufacturing has been accelerating. It results in that the data collection technologies from the shop-floor is becoming important. These approaches focus primarily on obtaining specific manufacturing data using various sensors and communication technologies. In order to expand the channel of field data collection, this study proposes a method to automatically collect manufacturing data based on vision-based artificial intelligence. This is to analyze real-time image information with the object detection and tracking technologies and to obtain manufacturing data. The research team collects object motion information for each frame by applying YOLO (You Only Look Once) and DeepSORT as object detection and tracking algorithms. Thereafter, the motion information is converted into two pieces of manufacturing data (production performance and time) through post-processing. A dynamically moving factory model is created to obtain training data for deep learning. In addition, operating scenarios are proposed to reproduce the shop-floor situation in the real world. The operating scenario assumes a flow-shop consisting of six facilities. As a result of collecting manufacturing data according to the operating scenarios, the accuracy was 96.3%.

Morphology of Barium Titanyl Oxalate Produced by Homogeneous Precipitation from Acidic Solution of Dimethyl Oxalate (Dimethyl Oxalate에 의한 균일 침전법으로 생성된 Barium Titanyl Oxalate의 형태학적 연구)

  • Min, Chonkyu;Lee, Chul
    • Analytical Science and Technology
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    • v.10 no.3
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    • pp.203-208
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    • 1997
  • Barium titanyl oxalate(BTO) was precipatated by utilizing the thermal decomposition of dimethyl oxalate in acidic aqueous solution having $BaCl_2$ and $TiCl_4$. Particle morphology of BTO was influeneced by the various experimental factors. i.e.. the faster rate to nucleation with higher temperature and the higher ratio of [DMO]/[$Ba^{2+}+Ti^{4+}$] was found to correspond to the faster rate of transformation of particle size distribution from unimodal to broad unimodal through bimodal. The BT powder obtained by calcination at $900^{\circ}C$ in air consists of larger particles than BT generated by general coprecipitation method and shows tetragonal symmetry. The stirring during reaction was also found to have much effect upon characteristics of BTO and BT.

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Curie Temperature and Tunable Dielectric Properties of Barium Strontium Titanate Thick Films (티탄산 바륨 스트론튬 (BaxSr1-xTiO3) 후막의 상전이온도와 가변 유전특성)

  • Jeon, So-Hyun;Kim, In-Sung;Min, Bok-Ki;Song, Jae-Sung;Yoon, Jon-Do
    • Journal of the Korean Ceramic Society
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    • v.43 no.7 s.290
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    • pp.421-426
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    • 2006
  • [ $(BaSr)TiO_3$ ] thick films were prepared by tape casting method, using $BaTiO_3\;and\;SrTiO_3$ powder slurry in order to investigate dielectric properties i.e. dielectric constant, ${\varepsilon}_r$, Curie temperature, $T_c$. Grain growth within $(BaSr)TiO_3$ thick films was observed with increasing weight ratio of $BaTiO_3$. This observation can be explained by phenomena of substitution of $Sr^{2+}$ ion for $Bi^{2+}$ ion in the $BaTiO_3$ system. Also, the Curie temperature in $(BaSr)TiO_3$ thick films was shifted to lower temperature range with increasing $ SrTiO_3$. Furthermore, Curie temperature having maximum dielectric constant was in the range of $-40^{\circ}C\;to\;30^{\circ}C$, and hence sharper phase transformation occurred at Curie temperature. There occurred decrease in tunability and k-factor of $(Ba_{0.6}Sr_{0.4})TiO_3$ calculated from the dielectric constant, ${\varepsilon}_r$ above Curie temperature. In addition, above the $60^{\circ}C$, phase fixation was observed. This means that internal stress relief occurred with increasing $90^{\circ}$ domains.

Effects of Herbal-acupuncture with Coicis Semen Solution at Joksamni (ST36) on Collagen-induced Arthritis in DBA/1J Mouse (족삼리(足三里) 의이인약침(薏苡仁藥鍼)이 Collagen으로 유발(誘發)된 생쥐의 관절염(關節炎)에 미치는 영향(影響))

  • Lee, Seong-No;Lee, Hyun
    • Korean Journal of Acupuncture
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    • v.24 no.4
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    • pp.111-129
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    • 2007
  • Objectives & Methods : The purpose of this study is to observe the effects of Coicis Semen Herbal-acupuncture solution (CS-HAS) at the Joksamni (ST36) on the collagen-induced arthritis in the DBA/1J mouse. The author performed several experiments to analyze the effects of CS-HAS on arthritis; change of the weight; the spleen size and adhesion rate; serum cytokine levels; serum antibody levels; changes of immunocyte counts; the histological changes of joint. Results : In the Coicis Semen Herbal-acupuncture (CS-HA), arthritis index, the incidence of arthritis, and the degree of joint edema were decreased. In CS-HA, there was no weight loss. The size of the spleen, adhesion rate, and the edema and transformation of joint were lowered. In CS-HA, the levels of IL-1${\beta}$, IL-6, TNF-${\alpha}$, IFN-${\gamma}$, IgG, IgM, and anti-collagen II in serum and the levels of IFN-${\gamma}$, IL-4, IL-10 in spleen were significantly decreased. In CS-HA, the expression ratios of $CD45^+$ to $CD3e^+$ and $CD8^+$ to $CD4^+$ were decreased. Also, the overall $CD4^+/CD8^+$ cell ratio was lowered in spleen. Ratios of the $CD4^+/CD25^+$, $CD45^+/CD69^+$ cells were decreased in lymph nodes. In addition, ratios of the $CD3^+/CD69^+$, $CD11b^+/Gr-1^+$ cells were also decreased in synovium. In the histological study, the cartilage destruction and synovial cell proliferation, and collagen fiber destruction were decreased with CS-HA treated group. Conclusions : From the results mentioned above, it is suggested that CS-HA at the ST36 has several significant effects on the collagen-induced arthritis.

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Evaluation of Fracture Toughness of Heat-Affected Zone in $9\%$ Ni Steel for Inner Wall of LNG Storage Tank (LNG 탱크 내조용 $9\%$ Ni 강 열영향부의 파괴인성 평가)

  • Jang J. I.;Yang Y. C.;Kim W. S.;Hong S. H.;Kwon D.
    • 한국가스학회:학술대회논문집
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    • 1997.09a
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    • pp.45-52
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    • 1997
  • The objective of this study is, with concept of fitness-for-purpose, to evaluate the fracture toughness in X-grooved weld HAZ(heat-affected zone) of QLT(quenching, lamellarizing and tempering)-processed $9\%$ Ni steel, qualitatively and quantitatively, and analyze the relation with the change of microstructure. In general, CTOD test is widely used to determine the fracture toughness of steel weldments. But several problem of accuracy has been brought up. Therefore, in this study, modified CTOD test was used for X-grooved weld HAZ for $9\%$ Ni steel. Additionally, microstructure of HAZ is observed and analyzed by OM, SEM and XRD. From the resulty, HAZ toughness of QLT-$9\%$ Ni steel decreased as the evaluated region approaches the fusion line. The decreased toughness was partly caused by reduction of the retained austenite content, resulted from decreased nucleation site of the retained austenite content, resulted from decreased nucleasion site for reverse transformation due to the increasing fraction of coarse grained region. On the other hand, unexpectedly, the increasing fraction of ductile weld did not increase the HAZ toughness. Therefore, in this X-grooved weld HAZ, the primary factor affecting fracture toughness was the fraction of coarse grained region, i.e., the weakest region.

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Synthesis and Characteristics of CU/CUO Nanopowders by Pulsed Wire Evaporativn(PWE) Method (전기폭발법에 의한 CU/CUO 나노분말의 제조 및 분말특성)

  • Maeng, D.Y.;Rhee, C.K.;Lee, N.H.;Park, J.H.;Kim, W.W.;Lee, E.G.
    • Korean Journal of Materials Research
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    • v.12 no.12
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    • pp.941-946
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    • 2002
  • Both Cu and Cu-oxide nanopowders have great potential as conductive paste, solid lubricant, effective catalysts and super conducting materials because of their unique properties compared with those of commercial micro-sized ones. In this study, Cu and Cu-oxide nanopowders were prepared by Pulsed Wire Evaporation (PWE) method which has been very useful for producing nanometer-sized metal, alloy and ceramic powders. In this process, the metal wire is explosively converted into ultrafine particles under high electric pulse current (between $10^4$ and $10^{ 6}$ $A/mm^2$) within a micro second time. To prevent full oxidations of Cu powder, the surface of powder has been slightly passivated with thin CuO layer. X-ray diffraction analysis has shown that pure Cu nanopowders were obtained at $N_2$ atmosphere. As the oxygen partial pressure increased in $N_2$ atmosphere, the gradual phase transformation occurred from Cu to $Cu_2$O and finally CuO nanopowders. The spherical Cu nanopowders had a uniform size distribution of about 100nm in diameter. The Cu-oxide nanopowders were less than 70nm with sphere-like shape and their mean particle size was 54nm. Smaller size of Cu-oxide nanopowders compared with that of the Cu nanopowders results from the secondary explosion of Cu nanopowders at oxygen atmosphere. Thin passivated oxygen layer on the Cu surface has been proved by XPS and HRPD.

Modified Bayesian personalized ranking for non-binary implicit feedback (비이진 내재적 피드백 자료를 위한 변형된 베이지안 개인화 순위 방법)

  • Kim, Dongwoo;Lee, Eun Ryung
    • The Korean Journal of Applied Statistics
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    • v.30 no.6
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    • pp.1015-1025
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    • 2017
  • Bayesian personalized ranking (BPR) is a state-of-the-art recommendation system techniques for implicit feedback data. Unfortunately, there might be a loss of information because the BPR model considers only the binary transformation of implicit feedback that is non-binary data in most cases. We propose a modified BPR method using a level of confidence based on the size or strength of implicit feedback to overcome this limitation. The proposed method is useful because it still has a structure of interpretable models for underlying personalized ranking i.e., personal pairwise preferences as in the BPR and that it is capable to reflect a numerical size or the strength of implicit feedback. We propose a computation algorithm based on stochastic gradient descent for the numerical implementation of our proposal. Furthermore, we also show the usefulness of our proposed method compared to ordinary BPR via an analysis of steam video games data.

Effect of Tempering Treatment on Mechanical Properties of Ausformed Martensite in Fe-30% Ni-0.35%C Alloy (Fe-30%Ni-0.35%C 합금에서 Ausformed Martensite의 기계적 성질에 미치는 Tempering처리의 영향)

  • Lee, E.K.;Lee, K.B.;Kim, H.S.
    • Journal of the Korean Society for Heat Treatment
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    • v.7 no.1
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    • pp.44-52
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    • 1994
  • In order to investigate the effect of tempring treatment on the mechanical properties of ausformed martensite in Fe-30%Ni-0.35%C alloy, the hardness, yield strength and elongation were examined by tensile test. 1. The strength of deformed austenite in Fe-30%Ni-0.35%C alloy was increased due to the work hardening induced from the dislocation density increased during deformation. The strength of ausformed martensite was increased because of defects inherited from deformed austenite by martensitic transformation. 2. The ductility of ausformed martensite was shown a nearly constant values independent of deformation degrees because of the interaction of multiple factors such as increased retained austenite, formation of void and decrement of twin in ausformed martensite. 3. The strength of ausformed martensite by tempering treatment was shown a little decrement up to $340^{\circ}C$, especially showed remarkable softening resistance in higher deformation degrees. 4. Virgin martensite and ausformed martensite were shown a maximum yield strength by clustering in tempering at $100^{\circ}C$ and above $100^{\circ}C$, yield strength was very small decreased due to the decrement of solute carbon by the destruction of clustering. 5. The decomposition of retained austenite was not shown up to $450^{\circ}C$ in ausformed martensite with tempering treatment, and the matrix was rapidly softening because of the decomposition of martensite and the formation of reversed austenite with tempering above $400^{\circ}C$.

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Semantic schema data processing using cache mechanism (캐쉬메카니즘을 이용한 시맨틱 스키마 데이터 처리)

  • Kim, Byung-Gon;Oh, Sung-Kyun
    • Journal of the Korea Society of Computer and Information
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    • v.16 no.3
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    • pp.89-97
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    • 2011
  • In semantic web information system like ontology that access distributed information from network, efficient query processing requires an advanced caching mechanism to reduce the query response time. P2P network system have become an important infra structure in web environment. In P2P network system, when the query is initiated, reducing the demand of data transformation to source peer is important aspect of efficient query processing. Caching of query and query result takes a particular advantage by adding or removing a query term. Many of the answers may already be cached and can be delivered to the user right away. In web environment, semantic caching method has been proposed which manages the cache as a collection of semantic regions. In this paper, we propose the semantic caching technique in cluster environment of peers. Especially, using schema data filtering technique and schema similarity cache replacement method, we enhanced the query processing efficiency.