• Title/Summary/Keyword: United Graduate School

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Strain Improvement for High Gentamicin Production Using Micromonosporas purpurea (Micromonosporas purpurea로부터 효율적 gentamicin 생산을 위한 균주 개발)

  • Choi DuBok;Yin Pemin;Choi On You;Shin Dae-Yewn
    • Journal of Environmental Health Sciences
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    • v.31 no.4 s.85
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    • pp.327-331
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    • 2005
  • Micromonosporas purpurea로부터 효율적 gentamicin 생산을 위해 protoplast fusion와 protoplast mutagenesis 방법이 검토 되었다. $CO^{60}\;irradiation\;(2.3{\times}10^5$ units, UV 3 min) 방법에 의해서 MP3-112, MP3-141, MP3-143을 분리 했다. 특히 MP3-143균주는 최대 gentamicin생산량이 얻어졌다. 개량된 MP3-143균주를 이용해서 탄소원 소비, 균체성장, 그리고 gentamicin 생산량이 batch culture에서 비교되었다. MP3-413와 parent 균주의 glucose 소비는 배양 2일과 3일 후에 각각 완전히 이루어졌다. 그러나 균체성장과 Soybean oil 소비는 비슷한 결과 얻어졌다. Gentamicin최대 생산량은 배양 5일 후 29756 U/ml였다. 이 결과는 parent 균주에 비해 생산량이 5.6배 증가했다.

The Application of Porter's Five Forces Model on the Electronic Components Industry in the United States and the Strategy of the Korean Company (미국 내 전자부품산업의 5 Forces Model과 한국기업전략)

  • Kang, Boksun;Lee, Minjung
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2022.01a
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    • pp.179-180
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    • 2022
  • 미상무성이 2021년에 발표한 미국의 전체 무역량 중 한국의 비중은 3.4%로 미국의 수입국중 7위를 기록하고 있다. 그중 전자부품이 차지하는 비율은 26.7%로 미국 내에서도 주요 수입품목으로 분류된다. KOTRA는 미국의 디지털화에 따른 5G, 6G, 클라우드 컴퓨팅, 인공지능(AI) 등의 산업 시장 확대와 더불어 소재부품의 수요도 증가할 것으로 전망하고 있다[1]. 한국의 부품산업이 미국 시장에 보다 효과적으로 진출하기 위한 전략 수립을 위해 마이클 포터의 5 Forces Model을 미국의 산업 환경에 적용하였다. 한국의 부품기업들이 미국시장진출을위해서는 국가 내부적으로는 원천 기술력을 최대한 확보하고, 원자재 공급망을 안정화시켜 외부의 위협을 견제함과 동시에, 미국 시장에서는 한국 전자부품의 차별성을 인증받아 소비재 개발의 초기단계에 파트너사로 진입 산업 내 경쟁우위를 선점하는 것이 중요하다.

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A Proposal of ccTLD Based on 'Corea', the Name of Our Country United (통일 국호 'COREA'를 가정한 국가도메인(ccTLD) 제안)

  • Hong, Seong-Hwan;Sim, Jae-Hwan;Choi, Jin-Young
    • Proceedings of the Korean Information Science Society Conference
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    • 2006.10d
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    • pp.571-575
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    • 2006
  • 통일에 대비한 남북한 인터넷 통합을 위한 준비작업으로서 통일 국가도메인 제정에 대한 논의가 계속되어 왔다. 분단상태의 남북한이 국가 도메인을 하나로 통합하여 공동으로 사용한다는 상징성이 크다는 점에서 남북한 모두가 동의할 수 있는 도메인을 제정하는 일은 중요하다. 통일 국가도메인에 대한 기존 제안들은 우리나라의 현 영문 국호인 'Korea'를 전제로 한 것들이었다. 논자는 일제 강점기 이전까지 우리 나라 영문 국호로 'Corea'가 사용되었다는 주장에 근거한 국호 영문표기 변경 논의가 남북 학술대회와 국회 결의안 등을 통해 공론화되어 많은 호응을 얻고 있음에 주목하였고, 남북한 국호의 영문표기 변경 논의와 연계하여 통일 국가도메인 제정을 추진하는 것이 시의적절하고 효율성도 높다고 판단하였다. 본 논문에서는 통일국가 도메인에 대한 기존 논의들을 검토하고 유사국가들의 국가도메인(ccTLD) 등록 사례를 통해 통일 국가도메인의 등록 가능성을 탐색한 후 통일 국가도메인명으로서 '.uc'(United Corea)를 최종 제안하였다.

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Analysis concerning the latest operating concept and status for Air Defense Identification Zone(ADIZ) (최근 방공식별구역 운영 개념과 현황 분석)

  • Kim, Dongsoo;Hong, Sungpyo;Chong, Mangseok
    • Journal of Aerospace System Engineering
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    • v.8 no.4
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    • pp.44-51
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    • 2014
  • This thesis analyzes the latest operating concept and status for Air Defense Identification Zone (ADIZ) researching overseas ADIZ CONOPS, international legal basis for ADIZ, the intention & background of proclamation for China Air Defense Identification Zone(CADIZ). Firstly, ADIZ is lawful concerning international connivance for ADIZ where around 20 countries have operated, Article 56 "Rights, jurisdiction & duties of the coastal State" and Article 301 "Peaceful uses of the seas" on the United Nations Convention on the Law of the Sea(UNCLS). Secondly, ADIZ has been regarded as a support means for national interest & policy as well as military air defense one. Thirdly, Based on legal re-interpretation for UNCLS relating to ADIZ, China proclaimed CADIZ where can ensure national maritime policy and strategy including A2/AD(Anti-Access & Area Defence), inroad into the ocean, claim for Senkaku Islands possession, etc..

Preparation of the Applicable Regulatory Guideline on Mixed Waste in Korea Based on the Analysis of US Laws and Regulations

  • Sim, Eun-Jin;Lee, Sun-Kee;Kim, Chang-Lak;Kim, Tae-Man
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.19 no.1
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    • pp.141-160
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    • 2021
  • Unit 1 of the Kori Nuclear Power Plant (NPP) and Unit 1 of the Wolsong NPP are being prepared for decommissioning; their decommissioning is expected to generate large amounts of intermediate-level, low-level, and very low level Waste. Mixed waste containing both radioactive and hazardous substances is expected to be produced. Nevertheless, laws and regulations, such as the Korean Nuclear Safety Act and Waste Management Act, do not define clear regulatory guidelines for mixed waste. However, the United States has strictly enforced regulations on mixed waste, focusing on the human health and environmental effects of its hazardous components. The U.S. Nuclear Regulatory Commission and the U.S. Department of Energy regulate the radioactive components of mixed waste under the Atomic Energy Act. The U.S. Environmental Protection Agency regulates the hazardous waste component of mixed waste under the Resource Conservation and Recovery Act. In this study, the laws, regulations, and authorities pertaining to mixed waste in the United States are reviewed. Through comparison and analysis with waste management laws and regulations in Korea, a treatment direction for mixed waste is suggested. Such a treatment for mixed waste will increase the efficiency of managing mixed waste when decommissioning NPPs in the near future.

The Strategy for Management of Plastic Waste in Korea through the Recycling Policy in Developed Countries (선진국의 폐플라스틱 재활용 정책에 의한 국내 관리 전략 마련)

  • Choi, Hyeong-Jin;Choi, Yong;Rhee, Seung-Whee
    • Journal of Korea Society of Waste Management
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    • v.35 no.8
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    • pp.709-720
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    • 2018
  • Since the import ban of plastic waste in China has been enforced, plastic wastes were not properly collected and recycled in Korea. Hence, the management strategies for plastic waste in Korea should be improved by examining the regulations and policy in developed countries such as United States, Japan, EU and United Kingdom. The management strategy for the recycling cycle should be implemented to expand the labeling system of separation and discharge, reduce the consumption of plastic products, automate the separation and sorting method in recycling facilities, and improve the economical efficiency of the recycling cycle. The concept of residual waste (secondary waste) in the material flow analysis should be implemented to identify the shortage point in the plastic waste stream. Finally, the cooperation with international communities is required for a transboundary movement of plastic waste, which includes participation at the working group of international standards to recycle plastic waste.

Comparison of Continuing Education Program for Emergency Medical Technician in Korea and Abroad (국내·외 응급구조사의 보수교육프로그램 비교조사)

  • Shin, Dong-Min;Jang, Mun-Sun;Kang, Bo-Ra;Yoon, Byeung-Gil;Tak, Yang Ju;Lee, In-Soo
    • The Korean Journal of Emergency Medical Services
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    • v.14 no.3
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    • pp.95-105
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    • 2010
  • Purpose: This study aims to investigate the operation of continuing education system and continuing education program for emergency medical technician in Korea, Japan and United States and develop reasonable operating scheme of continuing education and curriculum in order to provide the base data for the improvement of continuing education for the improved practice capability and its maintenance, Method: The overall review of continuing education for domestic 1st class emergency medical technician was performed and also the content of continuing education for Emergency Medical Technician - Paramedic in the United States and Paramedics of Fire Department with the license for the paramedic in Japan, have been analyzed through literature, books, articles, agencies' data, laws, and internet date. Result: Hours for domestic continuing education was 4-8 hours and it was only 3-11% compared to 72 hours in the United States and 128 hours in the Japan. And with respect to the types and methods of programs, there were differences both in quantity and quality. Conclusion: As an education, providing and supplementing the changed content and technical information for the improvement of the capability and qualification of emergency medical technician, selection of education hours and various continuing education should be continuously and regularly provided and conducted. The introduction of various continuing education system and programs for this is considered to be required.

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Variations in Growth Characteristics and Stress-wave Velocities of Zelkova serrata Trees from Eight Half-sib Families Planted in Three Different Initial Spacings

  • Prasetyo, Agung;Endo, Ryota;Takashima, Yuya;Aiso, Haruna;Hidayati, Fanny;Tanabe, Jun;Ishiguri, Futoshi;Iizuka, Kazuya;Yokota, Shinso
    • Journal of Forest and Environmental Science
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    • v.31 no.3
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    • pp.235-240
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    • 2015
  • Zelkova serrata is an important hardwood species for the timber industry in Japan. Tree breeding programs for this species have mainly focused on growth characteristics such as stem diameter (D), tree height (TH), stem form, and branching. In order to fulfill timber industry needs, wood quality improvement should be included in the tree breeding program of this species. In the present study, growth characteristics, such as D and TH, and the stress-wave velocity (SWV), which is highly correlated with Young's modulus of wood, were measured for 20-year-old Z. serrata from eight half-sib families planted in a progeny test site with three different initial spacings. Significant differences in all the measured characteristics were found among the eight half-sib families. The variance components of the half-sib families for D, TH, and SWV were 27.2%, 47.3%, and 33.5%, respectively. These results indicate that all the measured characteristics of this species could be improved by tree breeding programs. In addition, only low correlation coefficients were obtained between the growth characteristics and SWV, indicating that extensive selection on SWV in tree breeding programs may not always lead to a reduction in yield volume.

Production of Cloned Calves by the Transfer of Somatic Cells Derived from Frozen Tissues Using Simple Portable $CO_2$ Incubator

  • Dong, Y.J.;Bai, X.J.;Varisanga, M.D.;Mtango, N.R.;Otoi, T.;Rajamahendran, R.;Suzuki, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.2
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    • pp.168-173
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    • 2004
  • The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -20$-20^{\circ}C$, $-30^{\circ}C$ or $-80^{\circ}C$ in the presence of 5% DMSO used as tissue explants to generate somatic cells. The resultant confluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstracted from the somatic cells, 62.3%, 76.6% to 65% showed cleavage 70.5%, 81.9% to 78.5% reached the stage of morula formation and 39.7%, 43.2% or 47.6% reached the blastocyst stage. There was no significant difference in development when the NT embryos were compared with those reconstracted from fresh somatic cell derieved skin tissues (72%, 75.3%, and 45.2%, for cleavage, and development to morula and blastocyst stage, respectively). NT embryos were then placed in a portable $CO_2$ incubator and carried to China from Japan by air. After reaching to farm, two NT embryos were transferred to each of 5 recipients. We obtained 2 NT calves which birth weights is 30kg and 36kg female, and gestation periods is 281 and 284 days, respectively. There were no observation any abnormality from those calves. The results indicated that cell lines derieved from bovine fetal skin cryopreserved by a simple method could be used as donors in nuclear transfer using the portable $CO_2$ incubator.

THE EFFECT OF DIETARY FATS ON THE HEPATIC AND INTESTINAL 3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE ACTIVITIES IN CHICKS

  • Youn, B.S.;Tananka, K.;Ohtani, S.;Santoso, U.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.2
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    • pp.281-290
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    • 1993
  • This experiment was designed to evaluate the effect of degree of unsaturation (Experiment 1) and the chain length of constituent fatty acids of dietary fats (Experiment 2) on-3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activities in the liver and small intestine of chicks. Chicks were fed experimental diets for 10 days and then killed for the determination of the HMG-CoA reductase activities in the intestinal epithelial cell and hepatic microsomes. The hepatic HMG-CoA reductase activity showed the highest value in chicks fed the tallow-containing diet. Chicks fed diets containing safflower or coconut oil resulted in a significantly lower intestinal HMG-CoA reductase activity in comparison with those fed the olive oil-containing diet. The hepatic HMG-CoA reductase activity was significantly higher when fat-free and trilaurin were fed than when any other triglycerides were fed. This activity showed the lowest value in the chicks fed the diet containing tristearin. The HMG-CoA reductase activities in the jejunum and ileum were significantly or tended to be higher when trilaurin was fed than when any other triglycerides were fed. Except when trilaurin was fed, the presence of saturated fat in the diet did not have a significant effect on the intestinal HMG-CoA reductase activity, unlike the effect shown when a highly unsaturated fat was added to the diet. There was no significant correlation between the HMG-CoA reductase activities of the liver and intestinal, and the HMG-CoA reductase activity and cholesterol content of the intestinal epithelial cells.