• 대한물리치료과학회지
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• 제6권3호
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• pp.63-82
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• 1999

Recent studies have revealed that Nitric oxide(NO) was one of the demonstration for the physiological regulator, endothelial derived relaxing factor(EDRF) and that NO was produced by ultraviolet irradiation in human. Thus, the present author have carried out a experimental study on the change of hematological, histological value of ultraviolet irradiation in sprague-dawley rats. The subjects were divided into four groups of ten rats each selected at random. There were 4 groups: 1. no irradiation control; 2. ultraviolet $75mJ/cm^2$ irradiation group; 3. ultraviolet $150mJ/cm^2$ irradiation group; 4. ultraviolet $225mJ/cm^2$ group. After a irradiation, hematological and histological tests were performed to observe erythrocyte, hemoglobin, hematocrit, MCH, MCHC, MCV, $O_2$ saturation, pH, $PO_2,\;PCO_2$ value and to observe histological changes. In hematological tests, erythrocyte, hemoglobin, hematocrit significantly increased in $75mJ/cm^2$ than control group and more $150mJ/cm^2$ ultraviolet irradiation group respectively. Also In blood gas tests, $PO_2$ significantly increased in $75mJ/cm^2$ and more $150mJ/cm^2$ group than control group. Whereas $PCO_2$ significantly decreased in $75mJ/cm^2$ and more $150mJ/cm^2$ group than control group (Duncan-Tukey test, P<0.05). In histological tests, control and $75mJ/cm^2$ group unchanged, but more $150mJ/cm^2$ group changed that it was cytolysis, cytotoxic effect, acanthosis, proliferation of keratinocyte, appearance of amorphous cell and pyknotic nucleus, production of sunburn cell. In conclusion, the present author results support the importance of the relation between NO effect and hematological, histological value by ultraviolet B irradiation.

• 대한임상전기생리학회지
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• 제8권2호
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• pp.39-43
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• 2010

Purpose : The purpose of this study was to analyze skin reactions induced by ultraviolet irradiation using digital imagery. Methods : We recruited 15 women and ultraviolet irradiation was applied to their lumbar area. (The degree of inflammatory reaction was set on the basis of the third erythema dose. Image analysis was divided by Photoshop CS (8 bit RGB scale and gray scale). Then, images were processes using Image Pro Plus 4.5 program analyzing R, G, B, chromatic red value, luminance value and gray value. Results : As a result of analyzing changes in RGB scale, there were statistically significant differences in R, G, and chromatic red values. As a result of analyzing changes in gray scale, there were statistically significant differences in gray value. Analysis of changes in B and luminance values showed that there was no statistically significant difference. Conclusion : This study found that ultraviolet irradiation had influence on RGB and gray scale. These results suggest that changes to digital images on skin reaction by ultraviolet irradiation are related to erythema. In particular, these changes are related to R and gray values.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2001년도 춘계연합학술대회 초록
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• pp.195.2-195
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• 2001

• Toxicological Research
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• 제20권4호
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• pp.315-320
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• 2004

In this study we assessed the influences of ultraviolet (UV) light B radiation on epidermal ATPase-positive dendritic cell (DC) and the effect of green tea treatment in ICR mouse. The extent of changes following 200 mJ/$cm^2$ (0.5 mW/sec) was studied at 0, 6, 12, 18, 24, 30 or 36 hours after exposure. SBCs were decreased by 6 hours after irradiation. There was tendency to decrease from 6 hours to 24 hours and had little further change from then to 36 hours after irradiation. The mice that received 0, 50, 100, 200, 300 or 400 mJ/$cm^2$ of UVB were examined 24 hours after irradiation. The DCs were decreased as the radiation dose increases from 100 to 400 mJ/$cm^2$. The frequency of UVB (200 mJ/$cm^2$)-induced DC decrease was reduced by treatment of green tea (i.p. and topical application, p<0.01).

• Nutrition Research and Practice
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• 제1권4호
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• pp.279-284
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• 2007

Solar ultraviolet (UV) irradiation leads to distinct changes in the skin connective tissues by degradation of collagen, which is a major structural component in the extracellular matrix. UV irradiation induces the production of matrix metalloproteinases (MMP) capable of attacking native fibrillar collagen and responsible for inhibiting the construction of collagenous extracellular matrix. In this study, we attempted to investigate the protective actions of Rubus coreanus ethanol extract (RCE) on the MMP production and the consequent procollagen/collagen degradation in UV-B-irradiated human dermal fibroblasts. The analytical data showed that Rubus coreanus ethanol extract was mostly comprised of cyanidin 3-rutinoside. Pre-treatment of fibroblasts with this extract inhibited UV-B-induced production of MMP-1, MMP-8 and MMP-13 in dose-dependent manners. In addition, Western blot analysis and immunocytochemical staining assay revealed that RCE markedly augmented the cellular levels of procollagen/collagen declined in UV-B-exposed dermal fibroblasts. These results demonstrate that RCE blocks UV-B-induced increase of the collagen degradation by inhibiting MMP production. Thus, RCE may act as an agent inhibiting excessive dermal collagen degradation leading to the skin photoaging.

• 한국환경농학회지
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• 제15권4호
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• pp.448-454
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• 1996

본 연구는 자외선-B 조사(照射)가 피나무 유묘의 초기 생장에 미치는 영향을 알고자 수행되었다. 자외선-B는 유묘의 하배축 신장, 수고생장, 잎의 생장 및 엽록소 함량증가를 억제하였다. 이러한 억제 효과는 자외선-B 조사(照射)량이 증가할수록 더욱 높았다. 잎생장 감소 뿐만 아니라 형태적 변화도 나타났으며 엽장의 생장보다 엽폭의 생장이 빠르게 일어난 것이 관찰되었다. 자외선-B 처리를 받은 유묘의 1차엽 또는 2차엽에 변색, 반점, 반짝거림, 위축 또는 오그러듬의 피해증상이 관찰되었다.

• Applied Microscopy
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• 제36권2호
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• pp.109-118
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• 2006

자외선 B를 사람의 두피에서 채취한 모발에 6시간, 12시간, 24시간. 48시간 동안 각각 조사한 후 모발의 미세구조적인 변화 과정과 모발 단백질의 분해에 의한 원소 성분의 함량 변화를 전자현미경 및 에너지분산분광분석기를 이용하여 분석하였다. 자외선에 6시간 조사된 모발에서 모발 표면은 중첩된 큐티클층이 분리되어 있거나 부러져있는 상태로 나타났다. 큐티클세포의 내큐티클에는 직경이 30nm에서부터 700nm크기의 공포를 형성하고 있었다. 자외선에 12시간 조사된 모발에서 피질은 치밀하게 배열되어 있는 각화세포의 세포막이 파괴되어 공포가 형성되었는데 공포는 세포막을 따가서 일렬로 배열되어 있었다. 또한, 멜라닌 과립과 접촉하고 있는 거대원섬유에서 공포가 형성되어 멜라닌 과립과 분리되는 것을 확인할 수 있었다. 이 시기에도 멜라닌 과립의 모양이나 크기의 변화는 나타나지 않았다. 자외선에 24시간 조사된 모발에서 큐티클세포들은 절단된 상태로 관찰되었고 세포질의 내큐티클에 형성된 공포들은 인접해 형성된 공포들과 융합되었다. 자외선에 48시간 조사된 모발은 표면이 거칠게 나타났고, 큐티클의 안쪽 층에 있는 세포에서부터 형성된 공로로 인해 위쪽에 있는 큐티클 세포는 들뜨면서 조각나 떨어져 나갔다. 자외선 조사를 받아 손상된 모발의 원소성분을 에너지 분산 분광분석기로 분석한 결과 정상모발 보다 산소성분의 함량이 높게 나타났으나 황의 성분은 감소되었다.

• 한국잡초학회지
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• 제30권2호
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• pp.135-142
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• 2010

UV-B 처리가 봉선화 식물에 미치는 영향과 식물의 생화학적 방어반응을 조사하고자 3주간 UV-B (11.34 kJ $m^{-2}$) 조사 실험을 수행하였다. UV-B 처리에 의해 봉선화의 엽면적 및 건물중이 약 40% 정도 감소하였으며, MDA 함량은 50% 정도 증가한 것으로 나타났다. Glutathione 및 ascorbate acid 함량은 UV-B에 의해 산화형이 증가하고 환원형이 감소하였다. 봉선화 잎에는 주로 3종류의 polyamine이 존재하였으며, 3종류 모두 UV-B에 의해 증가하는 것으로 나타났다. 항산화효소인 SOD, AP, GR 및 GP의 활성이 UV-B 처리에 의해 크게 증가하였다. 이상의 결과로 볼 때 UV-B 증가는 식물체내 산화스트레스를 일으키며, 이에 대해 식물의 생화학적 방어반응이 작용하는 것으로 사료된다.

• Journal of Ginseng Research
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• 제30권4호
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• pp.188-193
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• 2006

We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of red ginseng (RG) on the formation, and decrease of UVB-induced epidermal mel-anocytes. C57BL/6 mice were irradiated by UVB $80mJ/cm^2$ (0.5 mW/sec) daily for 7 days, and RG was intraperitoneally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 $melanocytes/mm^2$, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with RG before each irradiation interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal mel-anocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with RG at 3rd and 6th weeks after irradiation. The present study suggests the RG as inhibitor of UVB-induced pigmentation and depigmenting agent.

• 한국환경과학회지
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• 제7권4호
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• pp.487-492
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• 1998

The experiment was conducted to determine the effects of enhanced UV-B on growth and differential responses among cultivars in soybean. The soybean cultivars subjected to enhanced UV-B irradiation at daily dose of 11.32 kJ $m^{-2}(UV-B_{BE})$ revealed that the growth was significantly depressed. Plant height, leaf number, leaf area and dry weight were inhibited by UV-B irradiation showing differential responses among cultivars used. Danyeubkong seems to be less sensitive to the enhanced W-B irradiation, while Keunolkong more sensitive. Reduction of chlorophyll content was also found significantly greater to Keunolkong. Specific leaf weight an index of leaf thickness, and flavonoid content known as UV-absorbing compounds were significantly Increased in Danyeubkong by UV-B, but those In the other cultivars were not significantly affected. The results indicated that there are cultivar diferences in tile growth and phisiological responses to the enhanced UV-B irradiation and specific leaf weight and UV-absorbing compounds in the leaves were highly related to the sensitivity of soybean by UV-B irradiation.