• Title/Summary/Keyword: Ultrastructure

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Ultrastructure of Capillaria hepatica (Syn. Calodium hepatica) Isolated from the Liver of Mouse Infected with Artificially Embryonated Eggs Collected from House Rats (Rattus norvegicus)

  • Min, Byoung-Hoon;Lee, Haeng-Sook;Kim, Soo-Jin;Joo, Kyoung-Hwan
    • Applied Microscopy
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    • v.43 no.4
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    • pp.146-154
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    • 2013
  • Capillaria hepatica (syn. Calodium hepatica) is a parasite found mainly in rodent liver. But, it has also been found in a wide variety of mammals, including humans. This worm is unique as it is the only nematode parasite that is embedded in the liver parenchyma of the host even during the adult stage of the life cycle. They produce eggs that elicit a marked granulomatous reaction that eventually destroys the worms. Fibrosis and lymphoplasmacytic inflammatory infiltration are often observed around adult nematodes embedded in the liver parenchyma of the host. For this reason, complete isolation of this slender worm and observation of the intact ultrastructure is very difficult. In this study, 10 intact whole worms (C. hepatica) were isolated from the liver of 3-week-old mouse after inoculation of artificially embryonated eggs collected from house rats (Rattus norvegicus). Their external structure of was observed with light and scanning electron microscopy. The length of the isolated female and male C. hepatica was approximately 69.60 mm and 36.92 mm, respectively. More detailed ultrastructure, including bacillary band, eggs and vulva in female and spicule and spicule sheath in male C. hepatica was also described.

Anatomical Ultrastructure of Spermatozoa of Paralichthys olivaceus (Paralichthyidae, Perciformes) from Korea (한국산 넙치 (Paralichthys olivaceus) 정자의 미세해부학적 구조)

  • Kim, Kgu-Hwan;Lee, Byung-Chan;Nam, Myung-Mo
    • Korean Journal of Ichthyology
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    • v.23 no.2
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    • pp.106-110
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    • 2011
  • Fine structure of spermatozoa of the bastard halibut (Paralichthys olivaceus) is described. Its structure is characterized by acrosomeless, having a spherical head region and a small mid piece containing five mitochondria disposed one row. The flagellum inserts centrally on the nucleus and has lateral ribbons. The present study supports the suggestion that spermatozoal ultrastructure is of taxonomic importance and may be a phylogenetic criterion in teleostei.

Effect of Dietary Vitamin A on Plasma Membrane Property and Ultrastructure in Ethanol -administered Rat Liver

  • Yang, Kyung-Mi;Seo, Jung-Sook;Cho, Hyun-Gug
    • Preventive Nutrition and Food Science
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    • v.3 no.1
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    • pp.56-61
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    • 1998
  • This study was conducted to investigate the effect of dietary supplementation of vitamin A on the membrane property and ultrastructure in ethanol-administered rat livers. Male Sprague-Dawley rats weighing of 130 ~150g were fed with experimental diets for 7 weeks. The diets contained different types of vitamin A which were $\beta$-carotene, retinyl acetate and retinoic acid. After feeding theexperimental diets for 7 weeks, a dose of 3.0g ethanol (30%, W/V)/kg B.W was injected to rats intraperitoneally. Control rats received 0.9% saline containing isocaloric sucrose instead of ethanol. Plasma membrane fluidity of liver decreased in rats fed with vitamin a -Deficient diet with ethanol as compared to that of control rats. Fluidity change of liver plasma membrane that ethanol had induced was influenced by dietary supplementation of vitamin A, but not influenced by the type of supplemented vitamin. A . The ultrastructural changed of hepatic mitrochondria were observed in some rats such as vitamin A-deficient rats with ethanol. Inadequate consumptionof vitamin A contributed to ultrastructural changes such as swelled mitochondria occurred by ethanol-induced hepatotoxicity. Although accurate mechanism involved in the plasma membrane-stabilizing effect of vitamin A is still unclear, dietary supplementation of vitamin A such as retinyl acetate is neede to modulate this change. The direct involvement of membrane property on the cell damage caused by ethanol treatment remains to be established.

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Spermatogenesis and Sperm Ultrastructure of the Marsh Clam (Corbicula japonica) (Bivalvia: Veneridae) (기수재첩 (Corbicula japonica)의 정자형성과정과 정자 미세구조)

  • LEE Jeong Yong;KIM Wan Ki;LEE Chae Sung;CHANG Young Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.4
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    • pp.281-286
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    • 2004
  • Spermatogenesis and sperm ultrastructure of the marsh clam (Corbicula japonica) were investigated by electron microscopic observations. Testis of the marsh clam consists of numerous spermatogenic follicle containing germ cells in the different developmental stages. Spermatogonia are located nearest the outer wall of the follicle, while spermtocytes and spermatids are positioned nearer to the lumen. Spematogonia are oval-shaped and about $5{\mu}m$ In diameter. Spermatogonia develop into spermatocyte, spermatid and spermatozoon. In the spermatid to about $2{\mu}m$ in diameter, cytoplasm decreases and mitochondria move to the base of the nucleus and fuse into several spheres, the centrioles become orthogonally oriented, a flagellum appears, and an acrosomal vesicle forms. The mature sperm has primitive type, consisting of a head, a midpiece and a tail. The sperm was arrow-shaped, and its head is about $8{\mu}m$ long and comprised of a long nucleus and an acrosome. The four mitochondria encircled the centrosome in midpiece. The flagellum had the classical 9+2 axoneme structure, and axonemal lateral fins in the tail were observed.