• Parasites, Hosts and Diseases
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• v.42 no.4
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• pp.151-157
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• 2004

Developmental surface ultrastructure of Macroorchis spinulosus was studied by scanning electron microscopy. One-day-old juvenile fluke was leaf-shaped and bent ventrally. Body surface was covered densely with peg-like spines and with cobblestone-like cytoplasmic processes. Ciliated sensory papillae were concentrated around oral sucker. Several unciliated sensory papillae occurred equidistantly on oral sucker and acetabulum. The ciliated papillae appeared in two longitudinal lines symmetric bilaterally on dorsal surface. On adult flukes, tegumantal spines became wider in middle of the body surface. The cytoplasmic processes differentiated into more fine velvety form. It is likely that the differentiated fine cytoplasmic processes are an increased absorptive surface to adult M. spinulosus. It is suggested that single pointed tegumental spines on anterior half of the body may be supportive for this fluke to migration.

• Applied Microscopy
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• v.28 no.3
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• pp.273-282
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• 1998

Ultrastructure of fertilized egg envelope in the tomato clown anemonefish (Amphiprion freatus) was examined by mean of light, scanning and transmission electron microscopies. The fertilized egg of tomato clown anemonefish was of non-transparent, ellipsoidal, adhesive and non-floted type, and there were numerous oil droplets in the yolk sac and adhesive filaments in the area of the animal pole. The outer surface of egg envelope was smooth. The fertilized egg envelope consisted of two distinct layers; a non-adhesive outer electron-dense layer and an inner layer, consisting of five horizontal low electron-dense lamellae alternating with the middle electron dense interlamellae.

• Fisheries and Aquatic Sciences
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• v.10 no.4
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• pp.196-199
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• 2007

The spermatozoa of Leiocassis nitidus are relatively simple cells composed of a spherical head, a short midpiece, and a tail, as in most Siluriformes. The ultrastructure is characterized by the following features: Acrosome absent, as in most teleosts; around nucleus about $1.8\;{\mu}m$ long, with a deep nuclear fossa containing the proximal and distal centrioles and mitochondria. Two centrioles approximately $180^{\circ}$ from each other; 10 or more mitochondria surrounding the axoneme (with a 9+2 microtubular pattern), arranged in two layers in the postnuclear cytoplasm and separated from the axoneme by the cytoplasmic canal. Two lateral fins on the same plane as the two central microtubules; doublets 3 and 8, which are ultrastructural characteristics of the sperma tail unlike other siluroids laking the lateral fins.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.1
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• pp.111-116
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• 2002

This study investigated quantitative changes in the spaces between and within myofibrils and the impact of high and low voltage electrical stimulation on muscle ultrastructure as seen in electron micrographs. In addition, the relationships of these spaces and the impact to meat tenderness were investigated. The degradation of myofibrils during aging appeared to be localized across the muscle fibre. Structural deterioration of muscle fibres was evident 1 day post-mortem, involving the weakening in the lateral integrity of the myofibrils and Z-disc regions. Meat tenderisation, as shown by objective measurements, coincided with these increases in degradation, as assessed by the sum of the gaps between and within myofibrils. The results showed that the total size of gaps between and within myofibrils can be used as an indicator of meat tenderization during aging, but that ultrastructural alteration in electrically stimulated muscle had little relationship with meat tenderness.

• ALGAE
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• v.25 no.1
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• pp.11-15
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• 2010

Two new genera of red algae, Madagascaria erythrocladioides West et Zuccarello and Pseudoerythrocladia kornmannii West et Kikuchi (Erythropeltidales, Compsopogonophyceae, Rhodophyta), were previously described using molecular analysis and confocal microscopy of isolates in laboratory culture. We examined the ultrastructure of both genera to compare with ultrastructure of other members of the class Compsopogonophyceae. Both genera had Golgi bodies not associated with mitochondria and chloroplasts with a peripheral encircling thylakoid similar to all other members of the class studied thus far. Confocal autofluorescence images showed that Madagascaria has a single round central pyrenoid while Pseudoerythrocladia has no pyrenoid. Our electron microscopic work confirms these initial observations. Tables and keys are presented that assist in interpreting cellular details of genera in the class Compsopogonophyceae.

• Applied Microscopy
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• v.25 no.1
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• pp.86-95
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• 1995

This study was accomplished to investigate the ultrastructure of mucous glands in dorsal skin of frog (Rana catesbeiana) by means of scanning and transmission electron microscopes. The dorsal skin of Rana catesbeiana is composed of epidermis and dermis. The cutaneous mucous glands consist of inner glandular epithelial cells and outer myoepithelial cells. Glandular epithelial cells are divided into four types by the microscopic ultrastructure; ER-rich cell, round secretory granule-containing cell, foam-like granule mass-containing cell, mitochondria-rich cell. Myoepithelial cell has a long elliptical nucleus and filled with fibrous materials in the cytoplasm. As a result of scanning microscopic observation, the surface of dorsal skin is covered with cutaneous protrusions. The opening sites of the mucous glands are irregularly distributed in dorsal skin.

• Journal of Microbiology
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• v.34 no.4
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• pp.379-383
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• 1996

Cryptosporidium parvum causes a life-threatening diarrhea in acquired immunodeficiency syndrome (AIDS) patients. THe sporozoite stage of C. parvum has been known to be a target in treating cryptosporidiosis in AIDS patients as it is an extracellular stage. A sporozoite was ultrastructurally observed. It has a creascent shape with a rounded posterior end and a tapering body. The compact nucleus was located at the posterior end. A monoclonal antibody was produced, which recognized a 43 kDa of sporozoite antigens in a western blot analysis and showed the surface labeling in immunofluorescence.

• 한국전자현미경학회:학술대회논문집
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• 1994.05a
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• pp.18-18
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• 1994

• 대한생식의학회:학술대회논문집
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• 2006.06a
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• pp.118-121
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• 2006

• Parasites, Hosts and Diseases
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• v.38 no.1
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• pp.9-15
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• 2000

A scanning electron microscopic study was performed on the surface ultrastructure of metacercariae and adults of Metagonimus takahashii. Metacercariae were collected from the scale of crucian carp (Carassius auratus) , and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae. the oral sucker had type I (numerous) and type II (seven in total) sensory papillae. Tegumental spines were dense and digitated into 5-7 points on the surface anterior to the ventral sucker, but became sparse and less digitated posteriorly toward the end of the body In adults, seven type II sensory papillae were characteristically arranged around the lip of the oral sucker, and on the inner side of the lip four small and two large type I sensory papillae were symmetrically seen on each side (12 in total). Tegumental spines on anterior two-thirds of the body. were digitated with 9-12 tips ventrally and 8-13 tips dorsally. Sperms entering into the Laurer's canal were observed. The results show that the surface ultrastructure of M. takuhashii is generally similar to those of M. yokogawai and M. miyatai except for the digitation of tegumental spines.