• Title/Summary/Keyword: Ultrastructural

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Ultrastructural Changes in the Cuticle of Palaemon serrifer (Palaemonidae, Crustacea) during the Intermolt Cycle

  • Park Jeung Sook
    • Fisheries and Aquatic Sciences
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    • v.2 no.1
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    • pp.78-84
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    • 1999
  • Ultrastructural changes in the cuticle of Paleamon serrifer associated with the intermolt cycle were examined and quantified as changes in the cuticular thickness. The cuticular thickness in each zoea stage increased with time elapsed after molting. The cuticle in the premolt stage was about 1.5 and 3 times thicker than that in the postmolt and intermolt stage, respectively. The cuticle in the premolt stage, including the molting space, was more than 5 times as thick as in the postmolt stage. In addition, newly hatched larvae were individually reared in the laboratory and body length for each specimen was measured frequently until the end of zoea VI. An average increase in body length between one zoea stage and the next is about $10\%$ of the length of the previous stage. Within individual zoea stages, the premolt stage had a body length some 0.3% longer than that of the postmolt stage, indicating a growth rate of about 0.03 mm/d.

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Study on the Cell Types of Epididymal Epithelium in the Rats (흰쥐 부정소 상피세포의 여러 유형에 관한 연구)

  • Cheong, Kyung-Soon;Park, Yong-Bin;Choi, Chee-Yong;Koh, Ki-Seok;Choe, Rim-Soon
    • Applied Microscopy
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    • v.20 no.2
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    • pp.23-36
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    • 1990
  • Several types of the epithelial cells were classified by ultrastructural observation through transmission electron microscope in the rat epididymis. Ultrastructural studies showed that the principal cells, basal cells and narrow cells are located in all the regions of the epididymis and the light cells are present only in the corpus and cauda epididymis. It was observed that the columnar epithelial cells like the principal cells, light cells and narrow cells contain several secretory vesicles and there are halo cells migrating in the several regions. The basal cells showed the elliptical forms in the caput and corpus region and the global forms in the cauda region.

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Histochemical and Ultrastructural Studios on the Cutaneous Mucous Glands of the Water Toad, Bufo stejnegeri Schmidt (물두꺼비(Bufo stejnegeri Schmidt) 피부(皮膚) 점액선(粘液腺)의 조직화학적(組織化學的) 및 미세구조적(微細構造的) 연구(硏究))

  • Do, Kum-Ryung;Moon, Myung-Jin;Kim, Chang-Whan;Kim, Woo-Kap
    • Applied Microscopy
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    • v.17 no.2
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    • pp.72-84
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    • 1987
  • Histochemical and ultrastructural properties on the cutaneous mucous glands of the water toad, Bufo stejnegeri Schmidt, are studied with light and electron microscopes. Mucous glands of the water toad are simple alveolar glands which are composed of gland cavity in dermis and gland duct in epidermis. Each gland cavity of the mucous glands is consisted of outmost myoepithelial cells, glandular epithelial cells and inner cavity. Gland cavities of the mucous glands are composed of four kinds of glandular epithelial cells which are the dark cell, the light cell, the mucous containing cell and the small cell. Secretory materials which are certified as acid mucopolysaccharides by the histochemical reactions are synthesized through the Golgi complexes of the mucous containing cells.

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Histochemical and Ultrastructural Study on the Stomach of a Land Snail, Nesiohelix samarangae (동양달팽이의 위에 대한 조직화학적 및 미세구조적 연구)

  • 정계헌;이용석
    • The Korean Journal of Malacology
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    • v.13 no.2
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    • pp.175-184
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    • 1997
  • A histochemical and ultrastructural study on the stomach epithelium of a land snail Nesiohelix samarangae was carried out during the period of June 1996 to May 1997. The stomach epithelium is simple columnar dpithelium and is consisted of three types of columnar cells, Type 1 cell which is majority in number has a brush border with microvilli on the free surface of the cell and contains numerous secretory granules supposed to be neutral mucopolysaccharide. Type 2 cell, elongated conical in shape. is rarely found in the dpithelium. This cell also has a brush border with microvilli on its free surface and contains well developed rough surfaced endoplasmic reticulum, Golgi bodies, and secretory granules in various electron densities. This cell seems to produce both of acid and neutral mucopolysaccharides. Type 3 cell, which is morphologically similar to the Type 1 cell, has microvili and cilia on the free surface and exists in group only in the limited regions of the stomach.

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Ultrastructural Changes and Formation of Storage Materials in Endosperm Cells during the Seed Formation of Panax ginseng C.A. Meyer (인삼(Panax ginseng C.A. Meyer)의 종자형성에 있어서 배유세포의 미세구조의 변화 및 저장물질의 형성)

  • 유성철
    • Journal of Plant Biology
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    • v.34 no.3
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    • pp.201-213
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    • 1991
  • This study has been carried out to investigate the ultrastructural changes, formation of storage materials in endosperm cells with electron microscope during the seed formation of Panax ginseng C.A. Meyer. In the early stage of seed formation with green seed coat, the endosperm was cellular type. Cell plate was largely composed of dictyosome vesicles in early stage of wall formation after mitosis. Central vacuole was gradually subdivided into several small-sized vacuoles. During the differentiation of plastids, some proplastid was replaced by amyloplast with starch grains and lamellar structure. A number of mitochondria with well developed cristae were distributed in cytoplasm. Rough endoplasmc reticulum, dictyosome, microbody, free ribosomes and polysomes were evenly distributed in cytoplasm. Spherical spherosomes were formed from dictyosome containing the lipid materials of even electron density. Protein bodies were formed by interfusing between vacuoles and vesicles derived from rough endoplasmic reticulum which contained the amorphous protein of high electron density.

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An Immunohistochemical and Ultrastructural Study of the Heavy Metals accumulated in the Kidney of an Antarctic Clam, Laternula elliptica (중금속이 축적된 남극 큰띠조개의 신장에 대한 면역조직학적 및 미세구조적 연구)

  • 이용석;안인영;김완종;정계헌
    • The Korean Journal of Malacology
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    • v.18 no.1
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    • pp.15-21
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    • 2002
  • The kidney of bivalve mollusks often contains remarkably high concentrations of both essential and non-essential metals and perform regulating and detoxicating activities. The kidney has also been proposed as a biological indicator for radioactive as well as for stable metals in the sea. The present study of the Antarctic clam, Laternula elliptica, concerns the functional morphology of the kidney epithelium, which contains highly accumulated heavy metals. The immunohistochemical and ultrastructural study was undertaken in order to find out the localization of metallothionein and heavy metals accumulated in the kidney of Laternula elliptica. In the immunohistochemical investigation, an intense metallothionein immunostaining reaction was found in the epithelial cells of the kidney of Laternula elliptica. Transmission electron microscopy showed that the epithelial cells contained numerous electron-dense inclusion bodies which were considered to be accumulated heavy metals.

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Ultrastructural Pattern of Synapses in the Rat Neostriatum during Postnatal Development (출생후 성장과정의 흰쥐 새줄무늬체에서 신경연접의 구조적 변동)

  • Lee, Hee-Lai
    • Applied Microscopy
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    • v.25 no.4
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    • pp.1-8
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    • 1995
  • This study was performed to analyze the morphological changes of synapses during early postnatal periods. Neonatal rats were grouped by 3, 7, 14, 21, 28 and 42day old, and observed the ultrastructural pattern of the synapses in the neostriatum by transmission electron microscope. 1. The number of synapse, the length of postsynaptic thickening and the amount of synaptic vesicles markedly increase during postnatal development 2. The proportion of asymmetric and curved synapses gradually increase by developmental periods. From the above results, it is suggested that the size of synapse increase during post-natal period, and asymmetric synapse are formed from the symmetric type and curved synapse are formed from the plane type.

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Ultrastructural Pattern of Synapses in the Rat Olfactory Bulb during Postnatal Development (성장기 흰쥐의 후각망울에서 신경연접의 구조적 변동)

  • Rho, Sook-Young;Lee, Hee-Lai
    • Applied Microscopy
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    • v.24 no.4
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    • pp.32-40
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    • 1994
  • This study was undertaken to study the morphological changes of rat synapses during early postnatal periods. Neonatal rats were grouped by 1st, 2nd, 3rd, 4th and 6th postnatal weeks, and we observed the ultrastructural pattern of the synapses in the external plexiform layer of olfactory bulbs by electron microscopy. The results were as follows; 1. The numbers of synapses, lengths of synaptic thickenings and amounts of synaptic vesicles were markedly increased in the external plexiform layer during four postnatal weeks. 2. There was a tendency of increasing the proportion of the asymmetric synapse and the curved synapses during maturation. From the above results, it is suggested that the size of synapse is increasing during maturation, and at the same time the asymmetric synapses are formed from the symmetric type and the curved synapses are from the plane type.

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