• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.8
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• pp.2117-2125
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• 2009

This paper describes an information system of operation adequacy in environmental facilities such as environmental contamination prevention and processing equipments, which is namely, an integrated management system for environmental facilities. By developing this system, we can improve environmental characteristics and maximize the efficiency throughout the life cycle from generation of contaminant source to contamination prevention processing, and to contaminant disappearance. In order to meet these objects, we have developed ubiquitous life cycle assessment(u-LCA) for environmental facilities, which is an ISO14001-based incineration facility information system. The developed system is currently operating for test at an incinerator in Koyang city. Due to this system, the managers can confirm the operation condition of the incinerator with real-time and they will be able to improve the problems immediately.

• Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.183-193
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• 2015

Several transgenic marine medaka Oryzias dancena strains harboring a green fluorescent protein (GFP) reporter construct regulated by an endogenous ${\beta}$-actin promoter were established and their expression characteristics in relation to transgene copy numbers were examined in 21 transgene genotypes. Most of the transgenic strains displayed transgene insertion patterns typical of microinjection-mediated introduction of foreign DNA into fish embryos, characterized by the random integration of multiple transgene copies (ranging from 1 - 282 copies per cell), often accompanied by the formation of concatemer(s), as assessed by genomic Southern blot hybridization analysis and qPCR. Transgenic strains showed ubiquitous and continued temporal and spatial expression patterns of the transgenic GFP during most of their life cycle, from the embryonic stage to adulthood, enabling assessment of the expression pattern of the endogenous ${\beta}$-actin gene. However, a comparative evaluation of transgene copy numbers and expression levels showed that copy number-dependent expression, the stability of the ubiquitous distribution and expression efficiency per transgene copy varied among the transgenic strains. Fluorescence expression levels were positively correlated with absolute transgene copy numbers, whereas the expression efficiency per transgene copy was inversely related to the number of transgene integrant copies. Data from this study will guide the selection of potentially desirable transgenic strains with ubiquitous expression of a fluorescent transgene, not only in this marine medaka species but also in other related model fish species.