• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1210-1217
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• 2021

Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242^T and B156^T, were isolated from soil in South Korea. Cells of strain H242^T were oxidase-positive and non-motile short rods, while those of strain B156^T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C_16:0, cyclo-C_17:0, andsummed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242^T and B156^T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242^T and B156^T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242^T and B156^T was 24.6%. Strains H242^T and B156^T were most closely related to Ramlibacter ginsenosidimutans BXN5-27^T and Ramlibacter monticola G-3-2^T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242^T and R. ginsenosidimutans and between strain B156^T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242^T and B156^T represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242^T (=KACC 21667^T=JCM 33922^T) and B156^T (=KACC 21665^T=JCM 33920^T), respectively.

• Journal of Microbiology and Biotechnology
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• 제33권10호
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• pp.1292-1298
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• 2023

PAMB 00755^T, a bacterial strain, was isolated from Korean fir leaves. The strain exhibits yellow colonies and consists of Gram-negative, non-motile, short rods or ovoid-shaped cells. It displays optimal growth conditions at 20℃, 0% NaCl, and pH 6.0. Results of 16S rRNA gene-based phylogenetic analyses showed that strain PAMB 00755^T was most closely related to Sphingomonas chungangi MAH-6^T (97.7%) and Sphingomonas polyaromaticivorans B2-7^T (97.4%), and ≤96.5% sequence similarity to other members of the genus Sphingomonas. The values of average nucleotide identity (79.9-81.3%), average amino acid identity (73.3-75.9%), and digital DNA-DNA hybridization (73.3-75.9%) were significantly lower than the threshold values for species boundaries; these overall genome-related indexes (OGRI) analyses indicated that the strain represents a novel species. Genomic analysis revealed that the strain has a 4.4-Mbp genome encoding 4,083 functional genes, while the DNA G+C content of the whole genome is 66.1%. The genome of strain PAMB 00755^T showed a putative carotenoid biosynthetic cluster responsible for its antioxidant activity. The respiratory quinone was identified as ubiquinone 10 (Q-10), while the major fatty acids in the profile were identified as C_18:1ω7c and/or C_18:1ω6c (summed feature 8). The major polar lipids of strain PAMB 00755^T were diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, and phosphatidylcholine. Based on a comprehensive analysis of genomic, phenotypic, and chemotaxonomic characteristics, we proposed the name Sphingomonas abietis sp. nov. for this novel species, with PAMB 00755^T as the type strain (= KCTC 92781^T = GDMCC 1.3779^T).

• 대한환경공학회지
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• 제30권8호
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• pp.831-838
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• 2008

본 연구는 호기조에 유동상 접촉여재를 충전한 실험실 규모의 A$_2$O 공법을 이용하여 낮은 C/N비(COD$_{Cr}$/T-N = 1.24) 하수처리시에 외부 탄소원 주입량 여부에 따른 탈질 및 미생물 군집구조 변화특성을 검토하였다. 미생물 군집구조 특성 분석은 퀴논분석을 이용하여 수행하였다. 실험으로부터 얻어진 결론은 다음과 같다. C/N비가 낮은 유입하수의 미생물 군집특성은 유비퀴논(UQ) 동족체 비율이 UQ-8 > UQ-10 > UQ-9 > 기타의 순으로 감소하고 메나퀴논(MK) 동족체는 UQ 동족체에 비해 존재비(f)가 적고 단순하였다. MK/UQ비는 0.41 이하, 비유사도(D)는 0.26 이하이었다. 외부탄소원 미주입 경우, 각 공정에서의 미생물 군집 특성은 혐기조에서는 퀴논종이 3종으로 MK-8이 우점이고 DQ와 EQ값이 작았다. 무산소조에서는 UQ/MK비가 2.3으로 호기성 미생물의 존재비가 높았다. 호기조에서는 부유성인 경우 우점퀴논이 UQ-7이며, 접촉여재 부착성인 경우 우점 퀴논이 UQ-8이었다. 외부탄소원을 주입한 경우, 미생물 군집 구조특성은 미주입시보다 혐기조에서 MK-8 비가 감소하고 UQ-8비, 퀴논종수, DQ값과 EQ값는 증가하였다. 외부탄소원 주입시 탈질향상은 주로 무산소조에서 일어났으며 UQ/MK비가 2.3에서 1.4으로 감소하였다. 이는 주로 UQ-8의 감소와 MK-7 및 MK-8의 증가에 의한 요인으로 미생물군집구조가 Pseudomonas sp.에서 Bacillus 및 Micrococcus sp. 같은 종의 구조로 변화함을 의미한다. 호기조에서 부유성인 경우 우점퀴논이 UQ-7에서 UQ-8으로, 부착성인 경우 UQ-8에서 UQ-10으로 변하였는데 이는 부유성에서 Nitrosomonas가 부착성에서 Nitrobactor의 존재비가 증가하였음을 의미한다.