• Environmental Analysis Health and Toxicology
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• v.20 no.2 s.49
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• pp.143-151
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• 2005

Exposure of skin to UV-B radiation can cause inflammatory response and immunosuppression. It has been reported that Korean Red Ginseng (KRG) has several pharmacological and physiological effects such as antioxidant, anticancer and improving immune function. In this study, we investigated that topical KRG and KRG + EGb 761 (Ginkgo biloba extract) combination prevented UV-B induced inflammation and inhibition of contact hypersensitivity response. Topical application of KRG, f days prior to or 5 days after exposure to 1MED and 2MED of UV-B, reduced skin thickness compared to non -treated group and resulted in protection against immunosuppression. However, KRG+EGb 761 combination has a little protection against the only 1MED UV-B. In conclusion. Topical application of KRG was more effective than combination in protection against UV-B induced inflammation and immune suppression. Also, we suggest that KRG can provide protection from inflammation and immunosuppression by UV-B radiation.

• Journal of Photoscience
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• v.9 no.2
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• pp.454-456
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• 2002

It is important to determine the action spectrum of UV-B radiation contained in the sunlight to estimate the risk of skin cancer. We have investigated action spectra for induction of apoptosis and reproductive cell death in L5178Y cells using the Okazaki Large Spectrograph at NIBB. L5178Y cells were exposed to light at different wavelengths in UV-B or UV-A region. Frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation, and by the colony formation assay, respectively. The measured sensitivity spectra for the two end-points were in very good agreement. Sensitivity decreased steeply with increase of wavelength in UV-B region and remains nearly constant in UV-A region. The action spectra were also slightly steeper than that for the minimum erythematic dose (MED), but very similar to the light absorption spectrum of DNA in UV-B region. On the other hand, the spectra for both endpoints were similar to MED spectrum but not DNA spectrum in the UV-A region. Also different time-course and morphological difference of apoptosis were found between UV-B (long time, fragmentation) and UV-A (short time, shrinkage) region. These results suggest that DNA damage induced by UV-B light triggers apoptosis and reproductive cell death, but other damaged targets (membrane, protein and so on) trigger these effects in UV-A region.

• Journal of Photoscience
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• v.9 no.2
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• pp.154-157
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• 2002

Direct, continuous, and accurate measurements of solar ultraviolet irradiance (290-400 nm: UVR) have been carried out since 1990, by using both band-spectral ultraviolet-B (290-320 nm: UV-B) and ultraviolet-A (320-400 nm: UV-A) radiometers at Tokai University in Hiratsuka, Japan (35$^{\circ}$N, 139$^{\circ}$E). From our observations, the following findings are provided: 1) an increasing trend in solar UV -B from Oct. 1990 to Sept. 2000; 2) a regional comparison of solar UVR in Japan; 3) the distinct characteristics of UV-B and UV-A irradiance, such as diffuse property, daily and seasonal variation; and 4) human body protection against solar UVR. An increasing 10-year trend in global solar UV - B in Hiratsuka corresponded to a decrease in the total ozone amount measured at Tsukuba (36$^{\circ}$N, 140$^{\circ}$E), giving supportive evidence for a direct link between these two parameters. Furthermore, a strong correlation was found between solar UV-B and total ozone amount from results of UVR measurements at four Tokai University monitoring stations dispersed throughout Japan. Additional results revealed different diffuse properties in global solar UV and in global solar total (300-3000 nm: Total) irradiances. For example, in the global UVR, the diffuse component was dominant: about 80 % independent of weather, with more than 60 % of global UV-B, and more than 50% of global UV-A with even a cloudless clear sky. On the other hand, the portion of the diffuse in the global total irradiance was very low, less than 10 % on a cloudless clear day. Daily and seasonal variations of UV -B and UV -A irradiances were found to be quite different, because of the marked dependence of UV -B irradiance on the atmospheric ozone amount. Moreover, UV -B irradiance showed large daily and seasonal variations: the ratio between maximum and minimum irradiances was more than 5. In contrast, the variation in UV-A was small: the ratio between maximum and minimum was less than 2. Three important facts are proposed concerning solar UVR protection of the human body: 1) the personal minimal erythema dose (MED); 2) gender based difference in MED values; and 3) proper colors for UVR protective clothing.

• Journal of Korean Society for Atmospheric Environment
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• v.22 no.2
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• pp.223-233
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• 2006

We analyzed the monthly and seasonal mean of the daily Erythemal Ultraviolet-B (EUV-B, $280{\sim}320nm$) irradiance operating in Pohang, Anmyeon, Gosan, Mokpo and Kangnung with UV-Biometer (Solar Light Co., Model No. 501) at clear-sky noon during the period from 1999 to 2004. Also, we investigated the seasonal and regional characteristics for the UV index over the Korean Peninsula. The daily maximum occurred near solar southing time and the highest monthly accumulated EUV-B irradiance appeared in July and August at each regional observatory. The monthly mean value of the clear-sky EUV-B irradiance in Pohang, Anmyeon, Gosan, Mokpo and Kangnung showed 196.6, 161.8, 221.9, $171.5mWm^{-2}\;and\;179.7mWm^{-2}$ near noon in July respectively. The annual mean value of the daily accumulated EUV-B irradiance in Pohang, Anmyeon, Gosan, Mokpo and Kangnung were 1.8, 2.1, 2.2, $1.8kJm^{-2}\;and\;1.5kJm^{-2}$ respectively. The UV Index (UVI) showed above UVI 7(High) more than 90 days during one year over the Korean Peninsula.

• Proceedings of the SCSK Conference
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• 1992.09a
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• pp.36-45
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• 1992

The dosal sites of 61 Koreans were exposed to increasing UVB doses in the 10mJ/$\textrm{cm}^2$ interval from 10mJ/$\textrm{cm}^2$ to 100mJ/$\textrm{cm}^2$ by solar-simulator, and at the 24hr after UV-B exposure we determined the first MED (Minimal Erythema Dose). For the precise measurements, we peformed the second exposures arround the first MED with 2.5mJ/$\textrm{cm}^2$ interval. The mean MED of all volunteers was 39.6 $\pm$ 15.7mJ/$\textrm{cm}^2$. As the distinction of sex, the means of male and female were 46.9 $\pm$ 18.1mJ/$\textrm{cm}^2$ and 31.5$\pm$8.5mJ/$\textrm{cm}^2$ respectively. According to the classification of skin type by Greiter. F.(1), the percent of skin type I to Ⅵ were 10.9%, 36.4%, 34.6%, 12.7%, 3.6%, and 1.8% respectively.

• Journal of Environmental Science International
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• v.15 no.8
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• pp.701-718
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• 2006

The average ratio of the daily UV-B to total solar (75) irradiance at Busan (35.23$^{\circ}$N, 129.07$^{\circ}$E) in Korea is found as 0.11%. There is also a high exponential relationship between hourly UV-B and total solar irradiance: UV-B=exp (a$\times$(75-b))(R$^2$=0.93). The daily variation of total ozone is compared with the UV-B irradiance at Pohang (36.03$^{\circ}$N, 129.40$^{\circ}$E) in Korea using the Total Ozone Mapping Spectrometer (TOMS) data during the period of May to July in 2005. The total ozone (TO) has been maintained to a decreasing trend since 1979, which leading to a negative correlation with the ground-level UV-B irradiance doting the given period of cloudless day: UV-B=239.23-0.056 TO (R$^2$=0.52). The statistical predictions of daily total ozone are analyzed by using the data of the Brewer spectrophotometer and TOMS in East Asia including the Korean peninsula. The long-term monthly averages of total ozone using the multiplicative seasonal AutoRegressive Integrated Moving Average (ARIMA) model are used to predict the hourly mean UV-B irradiance by interpolating the daily mean total ozone far the predicting period. We also can predict the next day's total ozone by using regression models based on the present day's total ozone by TOMS and the next day's predicted maximum air temperature by the Meteorological Mesoscale Model 5 (MM5). These predicted and observed total ozone amounts are used to input data of the parameterization model (PM) of hourly UV-B irradiance. The PM of UV-B irradiance is based on the main parameters such as cloudiness, solar zenith angle, total ozone, opacity of aerosols, altitude, and surface albedo. The input data for the model requires daily total ozone, hourly amount and type of cloud, visibility and air pressure. To simplify cloud effects in the model, the constant cloud transmittance are used. For example, the correlation coefficient of the PM using these cloud transmissivities is shown high in more than 0.91 for cloudy days in Busan, and the relative mean bias error (RMBE) and the relative root mean square error (RRMSE) are less than 21% and 27%, respectively. In this study, the daily variations of calculated and predicted UV-B irradiance are presented in high correlation coefficients of more than 0.86 at each monitoring site of the Korean peninsula as well as East Asia. The RMBE is within 10% of the mean measured hourly irradiance, and the RRMSE is within 15% for hourly irradiance, respectively. Although errors are present in cloud amounts and total ozone, the results are still acceptable.

• 대한예방의학회:학술대회논문집
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• 1996.10a
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• pp.197-198
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• 1996

• Proceedings of the Korean Society of Applied Pharmacology
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• 2003.11a
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• pp.110-110
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• 2003

It is well-documented that decreased antioxidant defense system by ultraviolet(UV) irradiation is the most important reason to induce the skin aging, especially photoaging. Chlorogenic acid(CA), a nonflavonoid catecholic compound, is present in the diet as part of fruits, tea, coffee and wine and has been reported to have anti-inflammatory, antimutagenic and anticarcinogenic activities. In this study, we examined the effects of CA on the UV -induced photoaging. Firstly, we investigated the protective effect of CA on antioxidant defense system in HaCaT human keratinocytes after UV irradiation treatment. UV irradiation decreased antioxidant defence enzyme activities of superoxide dismutase, catalase and GSH contents, which were restored by CA. To elucidate the effect of CA, 1% of CA and vehicle were applied to human buttock skin before and after UV irradiation (2MED). CA prevented UV -induced matrix metalloproteinase-1 mRNA expression and procollagen mRNA depression. And CA also increased CD1a(Langerhans cell) expression significantly. Our results suggest that CA has protective effects on UV -induced photoaging by increasing cellular antioxidant defense system. Therefore, CA may be a useful anti-aging agent for cosmetic purpose.

• Atmosphere
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• v.21 no.1
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• pp.123-130
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• 2011

In this study, we have analyzed the radiative characteristics of erythemal ultraviolet-B (EUV-B from 1999 to 2005) over the Korean Peninsula. EUV-B measured at Gangneung, Anmyondo, Mokpo, and Gosan represents the measurements from clean areas and that at Seoul represents from a polluted area. The magnitudes of EUV-B increase in proportion to the latitudinal decrease. Monthly mean variation of EUV-B at noon shows the maximum value of $158.5mWm^{-2}$ in August and the minimum value of $36.4mWm^{-2}$ in December in the clean areas. Seasonal mean diurnal variation of EUV-B shows a peak around noon (12:00 ~ 13:00 hr) and its intensity varies along with a season in order of summer > spring > fall > winter. The maximum value of $56.4mWm^{-2}$ in summer is three times higher than that in winter ($14.3mWm^{-2}$). The value of EUV-B in the polluted area is lower than that in the clean areas, resulting from the effects of the blocking, reflection, and scattering of EUV-B due to high concentrations of PM10. UV-B is an essential element to synthesize vitamin D in human body. 200 IU(International Unite) of vitamin D can be formed by an exposure of 6-10% of body surface area to 0.5 MED(Minimal Erythemal Dose). In order to form vitamin D, the calculated exposure times to EUV-B are 15 min. in spring, 12 min. in summer, 18 min. in fall, and 37 min. in winter for the clean areas and 16, 16, 24, and 37 min. for the polluted area.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.18 no.1
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• pp.81-98
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• 1992

Hairless mice (Skh:HR-1) exposed to single doses (0.5, 1.0 and 3. OMED) of UV-B radiation were displayed remarkable changes of barrier function and surface morphology. Trans- epidermal water loss (TEWL) as an index of barrier function was measured by evaporimeter, and wrinkle density (WD) as an index of morphological alteration was measured by image analyzer. Significant changes of TEWL were not observed in the control and 0. SMED group, but 1.OMED and 3. OMED groups noted significant difference. TEWL of 3. OMED group was rapidly increased to the 3rd day and decreased until the 14th day when it reached nearly to normal level, Time-courses of TBWL for 1. OMEB and 3. OMED groups displayed similar pattern, but different only in the magnitude. WD were significantly decreased during the 3rd-5th day in all of the irradiated groups and then increased during the last period to the 14th day, but did not recover the normal level at the 14th day. Time-courses of WD for all groups exhibited similarity, and were entirely dependent on the exposed doses. We also observed histological changes which included hyperplasia, sunburn cell (SBC) formation, accumulation of polymorphonuclear leukocyte (PMNs), and loss of collagen of UVB- exposed hairless mouse skin. Changes of TEWL and WD are helpful in understanding of epidermal and dermal damages by single exposure of UVB.