• Journal of Korean Ophthalmic Optics Society
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• v.12 no.3
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• pp.97-103
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• 2007

This study was investigated to find the proper UV-A blocking percentage that could protect the denaturation of catalase and superoxide dismutase (SOD), antioxidative enzymes in eye, induced by UV-A. Catalase or SOD were irradiated at 365 nm for 1, 3, 6, 24, 96 hr and the extent of denaturation was evalutated by polyacrylamide gel electrophoresis. Furthermore, it was investigated whether blocking of UV-A by 20, 50, 80 and 99% eyeglass lens could protect the denaturation of catalase and SOD or not. Catalase became to denature when catalase were irradiated by UV-A for more than 3 hours. However, the denaturation of SOD was induced by more than 6 hours irradiation. The denaturation of catalase induced by irradiation for 3 hr could be perfectly protected by 99% UV-A blocking lens. But, when the irradiation time became longer than 3 hr or the blocking percentage of lens were lower than 99%, the denaturation of catalase was not perfectly protected but partially protected. Although 50% UV-A blocking lens had partial protecting effects, lenses having 80 or 99% UV-A blocking effect could perfectly prevent the denaturation of SOD induced by 96 hr irradiation.

• Journal of Korean Ophthalmic Optics Society
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• v.12 no.1
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• pp.9-15
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• 2007

The aim of this study was to find the proper UV-A blocking percentage that could protect the denaturation of ribonuclease A (RNase A), one of protein enzymes in eye, induced by UV-A. RNase A was irradiated at 365 nm for 1, 3, 6, 24, 48, 72, 96 hr and the extent of denaturation was monitored by polyacrylamide gel electrophoresis. Furthermore, it was investigated whether blocking of UV-A by 20, 50, 80 and 99% eyeglass lens could protect the denaturation of RNase A or not. The denaturation of RNase A was induced by only 1 hr UV-A irradiation and the extent of denaturation became severe depending on UV-A irradiation time. The mild denaturation of RNase A induced by irradiation for 1 hr could be sufficiently protected by 20% UV-A blocking lens. When RNase A was irradiated for 3 hr, more that 50% blocking of UV-A needed to prevent the denaturation. Even though 99% UV-A blocking lens was used, the denaturation of RNase A induced by 6 hr irradiation could not be prevented perfectly. However, 99% UV-A blocking lens could dramatically decrease the severe denaturation of RNase A induced by irradiation for 96 hr.

• Journal of Korean Ophthalmic Optics Society
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• v.18 no.3
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• pp.297-304
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• 2013

Purpose: To investigate blue light rejection and the percentage of blue light in the visible light of blue light blocking lens according to light source, and to study prescriptions for blue light blocking lens Methods: UV-VIS spectrophotometer was used for blue light rejection and the percentage of blue light in the visible light according to light source, and the percentage of blue light in solar light was used to evaluate the prescription for blue light blocking lens. Results: The blue light rejection and the percentage of blue light in the visible light of each lens were depending on light sources. Through the way to compare the percentages of blue light in the visible light passing through the lens with that in solar light, blue light blocking lenses suitable to each light source could been selected. Conclusions: In the prescription for blue light blocking lens, inquiry for user's display must be preceded. And then the percentages of blue light in the visible light passing through the lens based on that in solar light may be useful as a method of evaluating the prescription for blue light blocking lens.