• Title/Summary/Keyword: UV/Vis spectrophotometric determination

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Spectrophotometric Determination of Amantadine Sulfate after Ion-Pairing with Methyl Orange

  • Choi, Kyong;Choi, Jung-Kap;Yoo, Gyurng-Soo
    • Archives of Pharmacal Research
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    • v.14 no.4
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    • pp.285-289
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    • 1991
  • A convenient spectrophotometric method was examined for the determination of amantadine sulfate (AMTS) which has no UV-VIS chromopohores. AMTS was ion-paired quantitatively with methyl orange (MO) at $70^{\circ}C$ for 30 min. The ion-paired complex was extracted with dichloromethane and the absorbance was measured at 421.5 nm. A linear relationship was observed in the range of $2.5{\times}10^{-7}\;M$ to $3.75{\times}10^{-6}\;M$ and the correlation coefficient was 0.999 (n=3). This assay method was applied to the quantification of AMTS in commercial tablet form with good recovery and high precision.

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The Analysis of Lanthanides Using UV-VIS Spectrometer (UV-VIS 흡광광도법으로 란탄족원소들의 정량분석에 관한 연구)

  • Ji, Bong Il;Kim, Ha Seok;Ha, Yeong Gu
    • Journal of the Korean Chemical Society
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    • v.34 no.6
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    • pp.616-621
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    • 1990
  • The spectrophotometric methods for the determination of Nd, Pr, Sm, Eu, Ho, Er were developed, using the multicomponent method of absorption spectra cointerference. The absorption spectra of individual Nd, Pr, Sm, Eu, Ho, Er, mixture of Sm, Eu, Ho, Er in hydrochloric acid solutions were investigated, and also a band register which includes the molar absorptions for all trivalent Nd, Pr, Sm, Eu, Ho and Er absorption band maxima were measured. The spectrophotometric determination of Nd, Pr, Sm, Eu, Ho and Er were investigated using the analytically significant absorption bands at 444.2, 575.1, 401.7, 394.2, 416.6, and 379.7 nm, respectively.

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Spectrophotometric Determination of Reducing Drugs (Ascorbic Acid, Potassium Antimonyl Tartrate, Isonicotinic Acid Hydrazide) with $Bis(2,4-diaminophenyl)phosphonate-KIO_3$ ($Bis(2,4-diaminophenyl)phosphonate-KIO_3$에 의한 환원성의약품(Ascorbic Acid, Potassium Antimonyl Tartrate, Isonicotinic Acid Hydrazide)의 분광광도정량)

  • Suh, Jung-Hyun;Kam, Sang-Kyu
    • YAKHAK HOEJI
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    • v.34 no.1
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    • pp.22-33
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    • 1990
  • Aqueous solution of bis(2,4-diaminophenyl)phosphonate(APP) was very stable, especially below pH 2.0 and the red-color compound formed by the reaction of APP and $IO_3-$ was stable at room temperature. A simple and rapid spectrophotometric method for the determination of ascorbic acid, potassium antimonyl tartrate (PAT), and isonicotinic acid hydrazide (INAH) was established by the reaction of $IO_3-$ and these reducing drugs, and the absorbance measurements were made at 500 nm. In the reaction of $IO_3-$ and each of the reducing drugs, the conditions of pH were suitable below 2.5 for ascorbic acid, below 2.0 for PAT, and below 1.5 for INAH. Beer's law did hold in the range of $17.6{\sim}1549.9\;ug$ for ascorbic acid, $33.4{\sim}2871.8{\mu}g$ for PAT,and $6.9{\sim}548.6\;{\mu}g$ for INAH. Many common ingredients present in pharmaceutical dosage forms did not interfere. The average recoveries for ascorbic acid and INAH in pharmaceutical formulations were 99.8 $-100.3\;{\pm}\;0.2{\sim}0.4%$, $99.8\;{\pm}\;0.3%$, respectively.

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Spectrophotometric Determination of Maximum Loading Capacity of a Dendrimer

  • Youngjin Jeon
    • Journal of the Korean Chemical Society
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    • v.67 no.4
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    • pp.217-221
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    • 2023
  • A series of hydrophobic dodecyl-terminated 6th-generation poly(amidoamine) dendrimer (H)-encapsulated cadmium sulfide ((CdS)n@H) nanoparticles in a co-solvent (toluene: methanol = 6.8: 3.2 v/v) are synthesized. The diameters of CdS nanoparticles within the dendrimer were estimated by analyzing the positions of the first excitonic absorption peaks of CdS in UV-vis spectra. The size of the CdS nanoparticle within the dendrimer shows a saturation value as the CdS/H ratio (n) increases, which is believed to be due to the limited physical size of the void cavity within the dendrimer. This simple and convenient method of estimating the saturation of the size of CdS in dendrimers may be useful in determining the maximum void space within other dendrimers under various solvent conditions.

A Chemometric Aided UV/Vis Spectroscopic Method for Kinetic Study of Additive Adsorption in Cellulose Fibers

  • Chal, Xin-Sheng;Zhou, Jinghong;Zhu, Hongxiang;Huang, Xiannan
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2006.06a
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    • pp.137-140
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    • 2006
  • This paper describes a technique combining chemometrics with UV spectroscopy for the determination of the concentra tions of two tissue additives (i.e., wet strength and softening agents) in a cellulose fiber containing solution. In single as ent solutions, the concentration of the additive can be measured by UV spectroscopy at the wavelength where the species having absorption. For a binary (i.e., containing two additives) solution system, the spectral characterization is very complicated. However, if aided by a chemometrical calibration technique, each additive in the binary solution can be quantified simultaneously. The present method is very rapid and simple, it can easily perform a continuous measurement in the changes in the additives' concentration after fiber addition, and therefore this becomes a valuable tool for the adsorption kinetics study of chemical additives onto the cellulose fibers. The time-dependent adsorption behaviors of the wet-strength, softening agent, and their both on fiber were also presented.

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Determination of Sesamin and Sesamolin in Sesame (Sesamum indicum L.) Seeds Using UV Spectrophotometer and HPLC

  • Kim, Kwan-Su;Lee, Jung-Ro;Lee, Joon-Seol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.1
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    • pp.95-100
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    • 2006
  • Sesamin and sesamolin, antioxidant lipidsoluble lignan compounds, are abundant in sesame (Sesamum indicum L.) seed oil and provide oxidative stability of oil related to sesame quality. The sesamin and sesamolin contents of 403 sesame land races of Korea were determined by HPLC analysis of methanol extract (HPLC value), and their total lignan content was compared with those by using UV-Vis spectrophotometric analysis (UV method) of methanol (UV-MeOH value) and hexane (UV-Hexane value) extracts. HPLC values of total lignan content were strongly associated with UV-Hexane (r=0.705**) and UV-MeOH (r=0.811**) values. The UV values from both the extracts were 3.8-4.7 times higher than those of HPLC values. Lignan content was overestimated by UV method because total compounds in the mixture solution were quantified by absorbing at the same ultraviolet wavelength as in HPLC method. UV method could more rapidly analyze small amount of sample with higher sensitivity of detection than HPLC method. Average contents of lignans in sesame germplasm evaluated in this study were $2.09{\pm}1.02mg/g$ of sesamin, and $1.65{\pm}0.61mg/g$ of sesamolin, respectively, showing significant variation for lignan components. The results showed that UV method for the determination of sesamin and sesamolin could be practically used as a faster and easier method than HPLC by using the regression equations developed in this study.

Determination of Total Phenol Content and Selected Phenolic Metabolites Analysis of Rice (Oryza sativa L.) Genetic Resources

  • Md Faruk Ahmed;Hee-Sung Moon;Yun-Ju Kim;Seung-Hyun Kim;Ill-Min Chung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2021.04a
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    • pp.137-137
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    • 2021
  • The study of total phenol (TP) content from 700 rice varieties was evaluated using the UV-Vis spectrophotometric method. The calibration curve of serial diluted gallic acid as a standard of this study showed the acceptable performances (R2 = 0.999, mean accuracy 90%) and the mean of % relative standard deviation (%RSD: 0.07%) within the range of 7.8 to 1000 ppm concentrations. The mean value of total phenol content from 700 rice varieties was 2723.15 ㎍/g ranged from 55.48 ㎍/g to 9922.23 ㎍/g and the mean %RSD was 2.5%. Furthermore, this study aim was to analyze and profile individual phenolic compounds in the rice genetic resources to construct an integrative database for development of new rice variety with high functionality for health and understanding of phenolics characteristics in the rice grain. Herein, we analyzed selected 100 rice varieties based on high TP content and identified total 15 phenolic compounds by LC-ESI-MS/MS. Among selected 100 rice genetic resources, the phenolic metabolites consisted of higher amount of flavonoid (catechin) and phenolic acid mainly protocatechuic acid. Further research of more selected rice genetic resources would be continued to provide for an integrative phenolics profile of different rice genetic resources.

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Simultaneous Spectrophotometric Determination of Cr(Ⅲ) and Cr(Ⅳ) in Chromium Plating Solution via Flow-Through Analysis (흐름계를 이용한 크롬도금 용액의 Cr(Ⅲ)와 Cr(Ⅵ)의 분광학적 동시 분석 방법)

  • Kim, Sun Kwan;Nam, Hakhyun;So, Jae Chun;Lee, Won;Cha, Geun Sig;Han, Sang Hyun
    • Journal of the Korean Chemical Society
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    • v.39 no.2
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    • pp.87-93
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    • 1995
  • The amount of Cr(Ⅲ) and Cr(Ⅵ) in a chromium plating solution could be determined simultaneously within five minutes using UV/VIS spectrophotometer coupled with a flow-through analysis(FTA) setup. The λmax's at 580 nm and 440 nm which correspond to Cr(Ⅲ) and Cr(Ⅵ), respectively, well obey the Beer-Lambert law in the range over 0.05 g/L for Cr(Ⅲ) and 0.005 g/L for Cr(Ⅵ). when the pH of the sample is less than 4.0, the absorption extinction coefficients of those peaks are not much dependent on the proton concentration. The amount of Cr(Ⅲ) and Cr(Ⅵ), and the total chromium in post treatment chromium plating solution, which normally has few g/L of Cr concentration, could be easily measured using UV/VIS spectrophotometry after the sample had been diluted with sulfuric acid of pH=1.0 in the flow-through analysis setup; this method is far more convenient and faster than the common redox titration (which requires at least 40 min/sample) method, while the accuracy of the method is highly comparable to conventional titrimetric method. Since the implementation of an PTA-UV/VIS setup in any plating industry is cost effective and easy to operate, the analytic method described in this paper may be applied directly for in situ analysis of plating solutions.

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Spectrophotometric Determination of Soil Chemical Properties Using Soiltek® KA-P Spectrophotometer (Soiltek KA-P 분광광도계률 사용한 토양 화학적 성질의 분광학적 분석)

  • Hyun, Hae-Nam;Oh, Sang-Sil;Koo, Bon-Jun;Kang, Ho-Jun
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.2
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    • pp.127-138
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    • 2000
  • To enable rapid and convenient soil test, new soil analytical methods, which require only one instrument, UV/Vis spectrophotometer, were developed and named "Soiltek KA-P spectrophotometric methods". The Soiltek$^{(R)}$ KA-P spectrophotometric method was compared with standard method of RDA in analytical capability for soil chemical properties. Using the 78 soils collected from upland, paddy, orchard, and vinyl house soils, soil organic matter, exchangeable K, Ca, and Mg. CEC, available $SiO_2$, and nitrate were analyzed by the two methods. The color stability(ratio of the absorbance at elapsed time t to the absorbance at time t=0) of organic matter. Ca, Mg, and available $SiO_2$ decreased to about 2% within one hour. However, that of exchangeable K, CEC, and nitrate remained constant. The results obtained with Soiltek$^{(R)}$ KA-P spectrophotometric method showed highly significant correlation with those measured by the standard method of RDA($R^2$ >0.9501), in which the slopes were near unity of $1.0{\pm}0.05$. The standard deviation values of organic matter, exchangeable K, Ca, and Mg, CEC, available $SiO_2$, and nitrate were apparently lower than ${\pm}1.8gkg^{-1}$, ${\pm}0.05cmol^+kg^{-1}$, ${\pm}0.18cmol^+kg^{-1}$, and ${\pm}0.13cmol^+kg^{-1}$, ${\pm}1.0cmol^+kg^{-1}$, ${\pm}5.0mgkg^{-1}$, and ${\pm}10.0mgkg^{-1}$, respectively. All the measurements showed coefficients of variation of less than 7~17% and were within the confidence level of 95%, which means both the methods are precise. Considering the relative simplicity, low cost, precision and accuracy, the proposed Soiltek$^{(R)}$ KA-P spectrophotometric methods could be recommended as an alternative to standard method.

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Studies on the Electrochemical Behaviors, Spectrophotometric Determination of Heavy Lanthanide Ions and Heavy Metal Chelate Complexes with Bidentate Ligands(III) -Synthesis and Characterization of the Tetrakis(5,7-dichloro-8-quinolinato)(2-mercaptopyrimidinato) molybdenum(IV) Complex- (무거운 란탄이온의 전기화학적 거동, 분광학적 정량 및 중금속 이온과 두 자리 리간드 착물에 관한 연구(제 3 보): -테트라키스(5,7-디클로로-8-퀴놀리나토)(2-메르캅토피리미디나토) 몰리브데늄(IV) 착물의 합성 및 특성-)

  • Chang, Choo Hwan;Choi, Won Jong;Park, Keun Su;Son, Pyung Su;Suh, Moo Yul
    • Analytical Science and Technology
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    • v.6 no.5
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    • pp.417-424
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    • 1993
  • Eight-coordinate tetrakis molybdenum(IV) complexes containing 5,7-dichloro-8-hydroxyquinolinol(Hdcq) and 2-mercaptopyrimidine(Hmpd) has been prepared. $Mo(mpd)_4$, $Mo(dcq)(mpd)_3$, $Mo(dcq)_2(mpd)_2$, $Mo(dcq)_3(mpd)$ and $Mo(dcq)_4$ complexes have been isolated by thin-layer chromatography on silicagel plates. These complexes have been charaterized by $^1H-nmr$ spectrum and UV-Vis. spectrum. The chemical shift values of the protons ${\alpha}$ to the nitrogen in the ligands are shifted to down field. The relative intensities of the peaks which are positioned at the same proton of $Mo(dcq)(mpd)_3$ and $Mo(dcq)_3(mpd)$ are observed in 2:1 ratio, in case of $Mo(dcq)_2(mpd)_2$ appears in approximately a 1:1 ratio. The stereochemistry of the complexes in discussed in terms of their nmr spectrum and Orgel's rule. By vertue of the intensities (${\varepsilon}$>10,000~25,000) the low energy($16,600{\sim}19,800cm^{-1}$) bands are observed for the electronic spectra of the complexes are assigned as charge transfer bands.

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