• Journal of Mushroom
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• v.15 no.3
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• pp.129-133
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• 2017

Oyster mushroom is a type of mushroom that is commonly cultivated and consumed in Korea. P. ostreatus 'Suhan' is a preferred cultivar for many mushroom farmers because it has a dark pileus and thick stipe. However, as it is very sensitive to environmental conditions, farmers consistently demand an alternative cultivar. To develop a new cultivar, the parental strains KMCC01680 ('Suhan') and KMCC00478 ('Gosol') were selected from various collected P. ostreatus strains by cultivating genetic resources. P. ostreatus 'Heuksol' was developed by the method of Mon-Mon crossing between monokaryotic strains derived from 'Suhan' and 'Gosol'. Thirty strains of 174 crossed strains were initially selected by cultivation experiments. After bulk cultivation tests, 'Heuksol' was selected. The nuclear DNA profile of 'Heuksol' was similar to those of the parental strains, 'Suhan' and 'Gosol', when RAPD (random amplified polymorphic DNA) primers and UPF (Universal PCR Fingerprinting) 2, 3, and 4 were used. The optimum temperature for mycelial growth was $30^{\circ}C$ for 'Heuksol', but medium-high temperatures were also appropriate, especially $13-20^{\circ}C$. The fruiting body production per bottle (1,100 mL) was approximately 140.8 g. When compared to the control strain 'Suhan', the thickness of the stipe of 'Heuksol' was greater than that of 'Suhan' (13.5 mm vs 9.4 mm). The pileus diameter of 'Heuksol' was similar to that of 'Suhan' and the pileus thickness of 'Heuksol' and 'Suhan' was 19.7 mm and 21.8 mm, respectively. 'Heuksol' had more a productive stipe number than 'Suhan' and the pileus of 'Heuksol' was dark gray, even at high temperatures. Therefore, it was suggested that this new cultivar, 'Heuksol', could provide an alternative to 'Suhan' and contribute to the profit of oyster mushroom farms.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.3
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• pp.405-415
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• 2000

Experiments for autotrophic denitrification were performed using an upf10w reac1.or packed with sulfur particles as an electron donor. The influent $NO_3{^-}$-N concentration was kept almost constant, but the hydraulic retention time(HRT) and temperature varied. Results of the research showed that the denitrification efficiency and gas generation rate decreased as the HRT and temperature were reduced. During the HRT effect experiment, alkalinities of 3.44~5.71g, with an average of 4.67 g which is close to the theoretical value of 4.57g were consumed for each gram of $NO_3{^-}$-N removed. During the temperature effect experiment, however, the values were 6.58~13.41 g with an average of 9.12 g which is almost twice the theoretical value Denitrification along the length of the reactor appeared to be a first-order reaction with a reaction rate constant of 0.1648/hr. On the other hand, the sulfate generation showed a zero-order reaction with a reaction rate constant of 241/hr. There was some discrepancy in the nitrogen mass balance between the theoretical and measured values, requiring further researches.