• Title/Summary/Keyword: UL122

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A Comparison between Low- and High-Passage Strains of Human CytomegalovirusS

  • Wang, Wen-Dan;Lee, Gyu-Cheol;Kim, Yu Young;Lee, Chan Hee
    • Journal of Microbiology and Biotechnology
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    • v.26 no.10
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    • pp.1800-1807
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    • 2016
  • To understand how human cytomegalovirus (HCMV) might change and evolve after reactivation, it is very important to understand how the nucleotide sequence of cultured HCMV changes after in vitro passaging in cell culture, and how these changes affect the genome of HCMV and the consequent variation in amino acid sequence. Strain JHC of HCMV was propagated in vitro for more than 40 passages and its biological and genetic changes were monitored. For each passage, real-time PCR was performed in order to determine the genome copy number, and a plaque assay was employed to get virus infection titers. The infectious virus titers gradually increased with passaging in cell culture, whereas the number of virus genome copies remained relatively unchanged. A linear correlation was observed between the passage number and the log10 infectious virus titer per virus genome copy number. To understand the genetic basis underlying the increase in HCMV infectivity with increasing passage, the whole-genome DNA sequence of the high-passage strain was determined and compared with the genome sequence of the low-passage strain. Out of 100 mutations found in the high-passage strain, only two were located in an open reading frame. A G-T substitution in the RL13 gene resulted in a nonsense mutation and caused an early stop. A G-A substitution in the UL122 gene generated an S-F nonsynonymous mutation. The mutations in the RL13 and UL122 genes might be related to the increase in virus infectivity, although the role of the mutations found in noncoding regions could not be excluded.

Impedance of Carbonized Path causing Arc Fault (아크사가 발생할 수 있는 고탄화경로의 임피던스)

  • Kwon, Wan-Sung;Choi, Su-Kyung;Bang, Sun-Bae;Kim, Chong-Min;Choe, Gyu-Ha
    • Proceedings of the KIPE Conference
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    • 2010.07a
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    • pp.121-122
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    • 2010
  • 전기회로에서 발생하는 아크로 인한 전기화재를 예방할 수 있는 아크차단기(AFCI)의 아크 검출성능 시험규격은 현재 UL1699가 세계적으로 인정되고 있다. 본 논문에서는 여기에 정의된 방법에 따라 탄화경로(Carbonized Path)를 생성하여 모의아크를 발생시켜 보았다. 그에 따른 전압을 가변하여 국내에서 사용되고 있는 전선 및 케이블에 탄화경로를 생성시킬 수 있는 적절한 전원을 제안하고, 아크가 발생한 탄화경로의 임피던스를 측정 및 분석함으로서 UL1699의 아크차단기 성능시험 방법을 검토하고 시험절차의 개선방법을 제안하였다.

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Structure and Bonding of Perovskites A($Cu_{1/3}Nb_{2/3}$)$O_3$ (A=Sr, Ba and Pb) and their Series of Mixed Perovskites

  • Park Hyu-Bum;Huh Hwang;Kim Si-Joong
    • Bulletin of the Korean Chemical Society
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    • v.13 no.2
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    • pp.122-127
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    • 1992
  • Some perovskites $A($Cu_{1}3}Nb_{2}3}$)O_3(A=$Sr^{2+}$$, $Ba^{2+}$ and $Pb^{2+}$) and their series of mixed perovskites have been prepared by solid state reaction. Single perovskite phase was obtained in Sr or Ba rich samples, but pyrochlore phase was found in Pb rich samples. The stability of perovskite phase is dependent on the ionicity of bonding as well as the tolerance factor. All the obtained perovskites have tetragonal symmetry distorted by Jahn-Teller effect of $Cu^{2+}$. In the case of $Sr(Cu_{1}3}Nb_{2}3})O_3$, some superlattice lines caused by threefold enlarging of fundamental unit cell were observed. And, the symmetry of B site octahedron and the bonding character of B-O bond have been studied by IR, ESR and diffuse reflection spectroscopy. It appeared that the symmetry and the bonding character are influenced by such factors as the size and the basicity of A cation.

Syphilis Screening Among some Industrial Workers in Korea

  • Yum, Yong-Tae
    • Journal of Preventive Medicine and Public Health
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    • v.11 no.1
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    • pp.110-122
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    • 1978
  • A study on the morbidity of syphilis among 729 workers in Po Hang and Ul San Industiral Complexes at the routine periodical examination was performed. In addition to the morbidity, factors related to the disease infection such as educational level, religion, preventive measures taken by the workers, history of urethritis, and so on were studied and analysed to check significant relation. The results were as follows: 1. VDRL and RPR tests were undertaken to determine the sensitivity and specificity of RPR test which was utilized for all the workers studied. It revealed 86.4% of sensitivity and 87.7% of specificity from RPR test, and 81.8% of sensitivity and 90.1% of specificity from the VDRL test compared with RPCF test. There were 87.4% of agreement rate between RPR and VDRL. Actually, RPR was more sensitive but less specific than VDRL, and found to be a satisfactory screening test for syphilis especially in the fields. 2. Among the workers in Po Hang Area 24.0% of them revealed reactive result, and in Ul San Area 5.2% revealed reactive result showing 4.6 times of risk among workers in Po Hang Area. 3. There were no statistically significant differences between RPR reactive rates and personal characteristics such as educational level, religion, history of urethritis experience, history of coitus with prostitute, and preventive measures of V.D. taken by workers if evaluated by area of Po Hang and Ul Srn separately. 4. V.D. could be encountered as an occupational disease in certain conditions of working places and the psychosocial problems of workers. And so, it is necessary that all the workers working in special estates such as in Po Hang Industrial Complex should be checked to be screened out by RPR test at periodical examinations and part of pre-employment examination also should include RPR test to be compared with. Regardless of the workmen's compensation, establishment of V.D. treatment clinic or system for such industrial workers is urgently needed. Health education regarding V.D. is another subject to be performed.

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Studies on the Differential Determination of cis-, and trans-Neopynamin and Fenitrothion by High Pressure Liguid Chromatography (고속 액체 크로마토그라피에 의한 cis- 및 trans-Neopynamin과 Fenitrothion의 분리 정량에 관한 연구)

  • 이왕규;정해수
    • YAKHAK HOEJI
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    • v.24 no.2
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    • pp.117-122
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    • 1980
  • A new application of high pressure liquid chromatography for the determination of fenitrothion and cis- and trans-neopynamin in insecticidal preparations was investigated. Optimum conditions for a good separation and determination were determined; solvent system: dichloromethane + n-hexane = 17 + 83; Bow rate: 0.5ml/min; column: u-porasil ($4mm{\times}3Ocm$); absorbance wavelength: 254nm; 0.05 AUFS and sample size: 30 ul. Recovery of fenicrothion, cis- and trans-neopynamin from mixed artificial preparations was 99.6%, 99.7% and 99.8% respectively. Also reproducibility tests showed that the coefficient of variation was 0.89% for fenitrothion, 0.74% cis-neopynamin and 1.1% for trans-neopynamin. There was no interference with insecticidal preparation containing DDVP, allethrin, S-421 and kerosene. HPLC method was rapid, accurate and it gave better reproducibility and higher sensitivity than any other analytical method. It was considered that HPLC could be greatly applied to the analysis of fenitrothion and neopynamin in insecticidal preparations.

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The Impact of COVID-19 on Health Prevention Behaviors in College Students: Focusing on the Health Belief Model (일부 대학생의 코로나19에 대한 건강예방행위에 미치는 영향: 건강신념모델을 중심으로)

  • Jo, Han-Ul;Choi, Eun-Hi
    • Journal of the Korean Society of School Health
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    • v.34 no.2
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    • pp.115-122
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    • 2021
  • Purpose: The purpose of this descriptive research is to identify how stress from Covid-19, health beliefs, and social support of college students affect health prevention. Methods: The subjects of the study were 128 university students, excluding health major students, at one university in D City. The survey was conducted from August 1 to 31, 2020. The survey questionnaire consists of 8 items on stress from COVID-19, 12 items adapted from a health belief measurement tool, 12 items from a social support measurement tool, and 11 items adapted from a tool that measures health preventive behaviors. The collected data were analyzed using the hierarchical multiple regression analysis method with SPSS 26.0. Results: In model 1, stress from COVID-19 was statistically significant (β=-.403, p=.003). Model 2 added four health belief factors into Model 1. Stress (β=-.419, p<.001), perceived severity (β=-.193, p=.030), and perceived barriers (β=-.182, p=.009) were statistically significant. In model 3, stress (β=-.413, p<.001), perceived barriers (β=-.147, p=.034), and social support (β=.194, p=.011) were statistically significant. The regression equation was significant (F=15.395, p=<.001) and the model's explanatory power was 53.1%. Conclusion: The results show that when college students had a high degree of health beliefs about COVID-19, the degree of health preventive behaviors was proportionally high. To make them practice preventive health behaviors, it is necessary to develop infection control education programs to improve health beliefs.