• 제목/요약/키워드: UL-700

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Behavior analysis of aerial tunnel maintenance truss platform with high tensile steel UL-700

  • Lee, Dongkyu
    • Steel and Composite Structures
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    • 제24권3호
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    • pp.383-391
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    • 2017
  • The goal of this study is to investigate structural analysis and behaviors of an innovative aerial work platform truss frame whose ductility is improved by using high strength-steel UL-700. The present space truss frame can move or stop through tunnels for maintenance constructions by automatic facilities and workmanship within standardized limited building lines of tunnel. Most of all, this method overcomes problematic, which is to block cars during construction periods, seriously, of typical methods like as using truck and scaffolds for tunnel maintenance. According to evaluated appropriate design results of space truss frames of numerical examples by using a commercial MIDAS GEN program, it is verified that design parameters such as layered size, cross-sectional size, and steel material of the present space truss frame are determined to depend on characteristics such as lanes or shape of road tunnels.

Behavior analysis of aerial tunnel maintenance truss platform with high tensile steel UL-700

  • Lee, Dongkyu
    • Steel and Composite Structures
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    • 제24권4호
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    • pp.513-521
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    • 2017
  • The goal of this study is to investigate structural analysis and behaviors of an innovative aerial work platform truss frame whose ductility is improved by using high strength-steel UL-700. The present space truss frame can move or stop through tunnels for maintenance constructions by automatic facilities and workmanship within standardized limited building lines of tunnel. Most of all, this method overcomes problematic, which is to block cars during construction periods, seriously, of typical methods like as using truck and scaffolds for tunnel maintenance. According to evaluated appropriate design results of space truss frames of numerical examples by using a commercial MIDAS GEN program, it is verified that design parameters such as layered size, cross-sectional size, and steel material of the present space truss frame are determined to depend on characteristics such as lanes or shape of road tunnels.

동국대학교 옥상녹화 지역의 식생 및 토양특성 변화 (Change of Vegetation and Soil Characteristics of Green Roofs in Dongguk University)

  • 이상진;박관수;김동일;이동근;길승호;장성완;박범환;윤준영;장관우;이호영;권오정
    • 한국환경복원기술학회지
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    • 제16권1호
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    • pp.193-206
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    • 2013
  • This study was to provide the base data on the status of vegetations and soils in green roofs by analyzing the soil and vegetation characteristics of 4 green roofs in Dongguk University in September 2012. Sanglokwon(SW), Dongguk Hall(DH), University Library(UL), and Information and Culture Hall P(IC) were established in 2005, 2008, 2009, and 2010, respectively. The areas of green roofs were $700m^2$, $2,300m^2$, $1,240m^2$, and $640m^2$ in SW, DH, UL, and IC respectively. The investigated floras of vascular plants were 26 families, 55 genera, 65 species in Sanglokwon(SW), 53 families, 99 genera, 112 species in Dongguk Hall(DH), 43 families, 77 genera, 84 species in University Library(UL), and 41 families, 71 genera, 75 species in Information and Culture Hall P(IC), respectively. A positive correlation is shown between the number of plant species and planting area. Total nitrogen, organic matter, and potassium in soil have positive correlation with the number of plant species. The number of plant species was proportional to area and increased more than twice after planting. About a quarter of the invaded plants (including native and naturalized species) were naturalized plants. The total soil depths including vegetation soil and drainage soil at SW, DH, UL, and IC were 20cm, 10cm, 10cm, and 8cm, respectively. The depths of vegetation soil at SW, DH, UL, and IC were <7cm, <3cm, <2cm, and <2cm respectively. The soil pH in vegetation soil ranged from 5.22 to 5.36, and from 6.13 to 6.39 in drainage soil. Available-P concentration ranged from 10.17 to 189.77mg/kg in vegetation soil and from 6.70 to 81.17mg/kg in drainage soil. Carbon concentration in vegetation soil ranged from 2.93 to 9.70%, and 2.93 to 9.70% in drainage soil. Carbon contents in 20cm, 10cm, 10cm, and 8cm soil depths were $2.62kg/m^2$, $1.89kg/m^2$, $0.50kg/m^2$, and $0.53kg/m^2$ at SW, DH, UL, and IC, respectively.

Analytical testing and evaluation of truss typed structures for tunnel maintenance

  • Lee, Dongkyu;Kim, Dohwan;Lee, Jaehong;Noh, Pilsung;Park, Sungsoo
    • Smart Structures and Systems
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    • 제15권4호
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    • pp.949-961
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    • 2015
  • The goal of this study is to present numerical modeling and analytical testing in order to evaluate an innovative space truss typed temporary structure, which is used to maintenance and repair of road tunnels. The present space truss structure has merits to use UL-700 high strength steel tube as members and to carry out maintenance and repair works of road tunnels without blocking cars and transportations. Numerical modeling and analytical testing of the space truss are investigated by using commercial engineering software, i.e., ABAQUS 6.5-1, and then it is verified that the truss structure has both structural safety and effective function for maintenances and repairs of road tunnels.

The phase angle dependences of Reflectance on Asteroid (25143) Itokawa from the Hayabusa Spacecraft Multi-band Imaging Camera(AMICA)

  • Lee, Mingyeong;Ishiguro, Masateru
    • 천문학회보
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    • 제40권1호
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    • pp.61.3-62
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    • 2015
  • Remote-sensing observation is one of the observation methods that provide valuable information, such as composition and surface physical conditions of solar system objects. The Hayabusa spacecraft succeeded in the first sample returning from a near-Earth asteroid, (25143) Itokawa. It has established a ground truth technique to connect between ordinary chondrite meteorites and S-type asteroids. One of the scientific observation instruments that Hayabusa carried, Asteroid Multi-band Imaging Camera(AMICA) has seven optical-near infrared filters (ul, b, v, w, x, p, and zs), taking more than 1400 images of Itokawa during the rendezvous phase. The reflectance of planetary body can provide valuable information of the surface properties, such as the optical aspect of asteroid surface at near zero phase angle (i.e. Sun-asteroid-observer's angle is nearly zero), light scattering on the surface, and surface roughness. However, only little information of the phase angle dependences of the reflectance of the asteroid is known so far. In this study, we investigated the phase angle dependences of Itokawa's surface to understand the surface properties in the solar phase angle of $0^{\circ}-40^{\circ}$ using AMICA images. About 700 images at the Hayabusa rendezvous phase were used for this study. In addition, we compared our result with those of several photometry models, Minnaert model, Lommel-Seeliger model, and Hapke model. At this conference, we focus on the AMICA's v-band data to compare with previous ground-based observation researches.

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仁川 沿岸 미세 생물 먹이망 내에서 浮游 原생動物의 포식율에 관한 연구 (The Study on the Grazing Rate of Protozooplankton tin the Microbial Food Web of Inchon Coastal Waters)

  • 최중기;김시균
    • 한국해양학회지
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    • 제30권5호
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    • pp.458-466
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    • 1995
  • 인천 연안 표영생태계의 미세생물 먹이 망에서 부유천생동물의 역할을 파악하기 위하여 시미세생물의 현종량과 성장률을 측정하고, 종속영양 편모충류와 섬모충류의 포식능력을 연구하였다. 종속영양 편모충류와 섬모충류의 여과율과 먹이섭취율은 형광 표지 박테리아(FLB)를 이용한 방법과 자연해시료를 분리 배양하는 방법에 의하여 연구 하였다. 본 조사시에 인천 연안수역의 박테리아 현존량은 0.7~1.5$\times$10/SUP 6/ cell. ml/SUP -1/, 종속영양 편모충류의 현종량은 1,680~2,180 cell.ml/SUP -1/, 독립영양 편모류의 현존량은 1.090~2,180 cell.ml/SUP -1/, 섬모충류의 현존량은 3,600~ 5,700 cell.ml/SUP -1/였다. 박테리아의 평균 성장률은 0.015~0.025 h/SUP -1/,섬모 충류의 성장률은 0.021 h/SUP -1/로 전반적으로 느린 편이었다. 종속 영양편모충류의 박테리아에 대한 포식률은 평균 0.055 h/SUP -1/로 높게 나타나고, 섬모충류의 종속영 양 편모류에 대한 포식률도 0.057 h/SUP -1/로 높게 나타났으나 독립영양 편모류에 대 한 포식률은 0.019 h/SUP -1/로 낮게 나타났다. 종속영양 편모충류의 박테리아에 대한 여과율과 섭취율은 각각 3.6~61.8 nl/SUP -1/.HFL.h/SUP -1/,6~52BAC.HFL.h/SUP -1/로 FLB를 이용하였을 때 낮게 나타나고 자연 개쳬군을 이용하였을 때 높게 나타나 며 박테리아 군집에 큰 영향을 주었다. 우점부유섬모충류인 Tintinnopsis sp의 평균여 과율은 3.2~4.6ul.CIL/SUP -1/.h/SUP -1/, 섭취율은 5~6 cells.CIL/SUP -1/. h/SUP -1/로 인천연안수역 편모류 현존량의 17-20%를 섭취하는 것으로 나타나고 해야 간조 시보다 만조 시에 다소 높게 나타났다.

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담배거세미나방(Spodoptera litura) Chitinase gene의 RNA interference (RNA Interference of Chitinase Gene in Spodoptera litura)

  • 전미진;서미자;윤영남;유용만
    • 농약과학회지
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    • 제18권3호
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    • pp.202-209
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    • 2014
  • RNA interference(RNAi)는 살아있는 세포 내에서 유전자의 표현 형을 억제하는 작용을 하고 Chitinase는 곤충이 탈피를 하는 동안 오래된 큐티클의 분해와 재흡수를 도와주는 효소로 알려져 있다. 이러한 작용기작을 이용하는 연구를 수행하기 위하여 담배거세미나방의 chitinase와 관련하여 탈피저해 효과를 조사하였다. 담배거세미나방 5령 유충으로부터 RNA를 추출하고 이용하여 cDNA를 합성하고 약 700 bp의 chitinase를 증폭 하였다. 증폭한 PCR product를 pGEM T-easy vector에 cloning하여 competent cell (E.coli)에 형질전환 시키고 mixture를 배양 후 colony를 선발하고 plasmid DNA를 추출하였다. 그 결과 약 3 kb size의 vector band와 약 700 bp의 insert band를 확인 할 수 있었다. dsRNA를 합성하기 위해 각각의 DNA를 Spe I과 Nco I의 제한 효소 처리를 하여 linear form의 DNA로 만들었다. dsRNA 합성 후 약 $10{\mu}g/{\mu}l$의 농도로 $5{\mu}l$씩 담배거세미나방 4령 유충에 주입하였다. 그 결과 유충-유충간의 탈피에서는 기형발육, 탈피저해, 표피의 색소 변이가 나타났다. 번데기-성충 간의 탈피에서는 탈피저해, 날개변이, 기형발육 현상을 볼 수 있었다. 용화율의 경우 무처리구 83.3%, DW 처리구 78.3%, dsRNA 처리구 66.7%로 나타났다. 우화율의 경우 무처리구 90.0%, DW 처리구 72.3%, dsRNA 처리구 65.0%로 나타나 dsRNA를 처리한 그룹에서 상대적인 탈피 저해 효과를 확인할 수 있었다. 그러나 변이율의 경우 무처리구 8.9%, DW 처리구 2.9%, dsRNA 처리구 19.2%로 dsRNA를 주입한 처리구에서 변이율이 가장 높게 나타난 것을 확인할 수 있었다. 표현형적 변이는 dsRNA 주입 후 약 18 시간 이후부터 뚜렷하게 나타나는 것을 볼 수 있었다.

Partial Sequencing and Characterization of Porcine DNA Methyltransferase I cDNA

  • Lee, Y.Y.;Kim, M.S.;Park, J.J.;H.Y. Kang;Y.M. Chang;Yoon, J.T.;K.S. Min
    • 한국발생생물학회:학술대회논문집
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    • 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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    • pp.84-84
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    • 2003
  • DNA methylation is involved in epigenetic processes such as X-chromosome inactivation, imprinting and silencing of transposons. DNA methylation is a highly plastic and critical component of mammalian development The DNA methyltransferases (Dnmts) are responsible for the generation of genomic methylation patterns, which lead to transcriptional silencing. The maintenance DNA methyltransferase enzyme, Dnmt 1, and the de novo methyltransferase, Dnmt3a and Dnmt3b, are indispensable for development because mice homozygous for the targeted disruption of any of these genes are not viable. The occurrence of DNA methylation is not random, and it can result in gene silencing The mechanisms underlying these processes are poorly understood. It is well established that DNA methylation and histone deacetylation operate along a common mechanistic pathway to repress transcription through the action of methyl-binding domain proteins (MBDs), which are components of, or recruit, histone deacetylase (HDAC) complexes to methylated DNA. As a basis for future studies on the role of the DNA-methyl-transferase in porcine development, we have isolated and characterized a partial cDNA coding for the porcine Dnmt1. Total RNA of testis, lung and ovary was isolated with TRlzol according to the manufacture's specifications. 5 ug of total RNA was reverse transcribed with Super Script II in the presence of porcine Dnmt 1 specific primers. Standard PCRs were performed in a total volume of 50 ul with cDNA as template. Two DNA fragmenets in different position were produced about 700bp, 1500bp and were cloned into pCR II-TOPO according to the manufacture's specification. Assembly of all sequences resulted in a cDNA from 158bp of 5'to 4861bp of 3'compare with the known human maintenance methyltransferase. Now, we are cloning the unknown Dnmt 1 region by 5'-RACE method and expression of Dnmt 1 in tissues from adult porcine animals.

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