• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1751-1757
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• 2007

Elastin-like polypeptides (ELPs) undergo a reversible inverse phase transition upon a change in temperature. This thermally triggered phase transition allows for a simple and rapid means of purifying a fusion protein. Recovery of ELPs-tagged fusion protein was easily achieved by aggregation, triggered either by raising temperature or by adding salt. In this study, levansucrase has been used as a model enzyme in the development of a simple one-step purification method using ELPs. The levansucrase gene cloned from Pseudomonas aurantiaca S-4380 was tagged with various sizes of ELPs to functionally express and optimize the purification of levansucrase. One of two ELPs, ELP[V-20] or ELP[V-40], was fused at the C-terminus of the levansucrase gene. A levansucrase-ELP fusion protein was expressed in Escherichia coli $DH5{\alpha}$ at $37^{\circ}C$ for 18 h. The molecular masses of levansucrase-ELP[V-20] and levansucrase-ELP[V-40] were determined as 56 kDa and 65 kDa, respectively. The phase transition of levansucrase-ELP[V-20] occurred at $20^{\circ}C$ in 50 mM Tris-Cl (pH 8) buffer with 3 M NaCl added, whereas the phase transition temperature ($T_t$) of levansucrase-ELP[V-40] was $17^{\circ}C$ with 2 M NaCl. Levansucrase was successfully purified using the phase transition characteristics of ELPs, with a recovery yield of higher than 80%, as verified by SDS-PAGE. The specific activity was measured spectrophotometrically to be 173 U/mg and 171 U/mg for levansucrase-ELP[V-20] and levansucrase-ELP[V-40], respectively, implying that the ELP-tagging system provides an efficient one-step separation method for protein purification.

• 한국정보통신학회논문지
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• 제9권5호
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• pp.1149-1160
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• 2005

일반적으로 네트워크 설계 문제는 네트워크의 크기가 늘어남에 따라 지수적으로 복잡도가 증가하여 전통적인 방법으로는 풀이하기 힘든 NP-hard 조합 최적화 문제 중의 하나로 분류될 수 있다. 본 논문에서는 네트워크 신뢰도 제약을 고려하면서 네트워크 구축비용을 효과적으로 최소화하는, 높은 신뢰도의 네트워크 토폴로지 설계 문제를 풀기 위해 스패닝 트리를 효율적으로 표현할 수 있는 $Pr\ddot{u}fer$수(PN) 기반의 진화 연산법과 2-연결성을 고려하는 휴리스틱 방법으로 구성된 두 단계의 효율적인 해법을 제안한다. 즉, 먼저 스패닝 트리를 찾아내기 위해 진화 연산법 중에 보편적으로 널리 알려져 있는 유전자 알고리즘(GA)을 이용하고 그 다음으로 첫 번째 단계에서 발견한 스패닝 트리에 대해 최적의 네트워크 토폴로지를 찾기 위해서 2-연결성을 고려한 휴리스틱 방법을 적용한다. 마지막으로 수치예의 결과를 통해 제안한 해법의 성능에 대해서 살펴보도록 한다.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회/대한산업공학회 2005년도 춘계공동학술대회 발표논문
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• pp.497-505
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• 2005

RFID를 활용하여 기업 공급망의 효율성을 향상하고자 하는 기업들이 늘고 있지만, 이들이 적용할 만한 가이드라인이 부재한 실정이다. 이에 대하여 본 연구에서는 물류/유통산업의 RFID 기반 u-Business 전략계획 방법론을 제시하였다. 본 방법론은 기존의 정보화 전략계획 방법론의 기본 절차를 따라 '도입전략 수립-현행체계 분석-목표체계 수립-통합 이행계획 수립'의 단계(Phase)를 기반으로 하여, 유비쿼터스 컴퓨팅 환경의 적용을 위해 기술도입 타당성 분석, 공급망 분석, 목표기업 벤치마킹, Pilot테스트, u-Business 전략계획 평가에 관하여 특화되었다. 제시된 방법론은 기존의 정보화 전략계획 방법론과의 비교를 통하여 타당성을 검증하였다.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1996년도 춘계학술발표회논문집(3)
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• pp.213-218
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• 1996

Characteristics of high density U-Mo alloy powder prepared by centrifugal atomization have been examined. The results indicate that the majority of the atomized U-Mo alloy particles has a smooth surface and frequently near-perfect spheroidal shape with few satellites attached. The size distribution of atomized U-Mo alloy powder shows the mono-modal size distribution seen in ligament disintegration mechanism. All phases of atomized alloy powder below 150$\mu\textrm{m}$ irrespectively to particle size are found to be ${\gamma}$-U (cubic structure) phases with isotropic structure and not to be U$_2$Mo phase at all. The microstructure of atomized U-Mo alloy particulates has micro-crystalline structure with non-dendritic gram supersaturated with Mo element. Also the grain size of ${\gamma}$ -U tends to decrease with the decrease of the powder diameter.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권3호
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• pp.962-975
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• 2020

The accurate segmentation of infant brain MR image into white matter (WM), gray matter (GM), and cerebrospinal fluid (CSF) is very important for early studying of brain growing patterns and morphological changes in neurodevelopmental disorders. Because of inherent myelination and maturation process, the WM and GM of babies (between 6 and 9 months of age) exhibit similar intensity levels in both T1-weighted (T1w) and T2-weighted (T2w) MR images in the isointense phase, which makes brain tissue segmentation very difficult. We propose a deep network architecture based on U-Net, called Triple Residual Multiscale Fully Convolutional Network (TRMFCN), whose structure exists three gates of input and inserts two blocks: residual multiscale block and concatenate block. We solved some difficulties and completed the segmentation task with the model. Our model outperforms the U-Net and some cutting-edge deep networks based on U-Net in evaluation of WM, GM and CSF. The data set we used for training and testing comes from iSeg-2017 challenge (http://iseg2017.web.unc.edu).

• Korea-Australia Rheology Journal
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• 제13권4호
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• pp.189-196
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• 2001

The phase morphology is an important factor in the rheology of immiscible polymer blends. Through its size and shape, the interface between the two phases determines how the components and the interface itself will contribute to the global stresses. Rheological measurements have been used successfully in the past to probe the morphological changes in model blends, particularly for dilute systems. For more concentrated blends only a limited amount of systematic rheological data is available. Here, viscosities and first normal stress differences are presented for a system with nearly Newtonian components, the whole concentration range is covered. The constituent polymers are PDMS and PIB, their viscosity ratio can be changed by varying the temperature. The data reported here have been obtained at 287 K where the viscosities of the two components are identical. By means of relaxation experiments the measured stresses are decomposed into component and interfacial contributions. The concentration dependence is quite different for the two types of contribution. Except for the component contributions to the shear stresses there is no clear indication of the phase inversion. Plotting either the interfacial shear or normal stresses as a function of composition produces in some cases two maxima. The relaxation times of these stresses display a similar concentration dependence. Although the components have the same viscosity, the stress-component curves are not symmetrical with respect to the 50/50 blend. A slight elasticity of one of the components seems to be the cause of this effect. The data for the more concentrated blends at higher shear rates are associated with a fibrillar morphology.

• E2M - 전기 전자와 첨단 소재
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• 제7권4호
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• pp.289-293
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• 1994

Thin films of YB $a_{2}$C $u_{3}$$O_{7-x}$ supercondYB $a_{2}$C $u_{3}$$O_{7-x}$uctor were prepared on (100) SrTi $O_{3}$ substrates by pulsed laser deposition using visible light laser. Q-switched Nd:YAG(532 nm, 30 ns) pulsed laser was used for deposition. The effects of substrate temperature and oxygen pressure during deposition on films were studied. Critical current density of 2.93*10$^{6}$ A/c $m^{2}$ at 77K and Tc(zero)=91.7K were obtained from the film prepared with Tsub=745.deg. C and $P_{02}$=200 mTorr. XRD analysis showed that the grown film has c-axis normal orientation to the substrate surface and has single phase. Surface morphology of the film has been improved by interfering the plume ejected from YB $a_{2}$C $u_{3}$$O_{7-x}$ target.arget.t.

• 한국환경성돌연변이발암원학회지
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• 제17권1호
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• pp.7-11
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• 1997

Variations in adriamycin uptake and cytotoxicity were studied in tumor cells that were grown in different growth states and microenvironments. RIF-1 tumor cells were maintained in an RPMI 1640 medium, and grown in either a monolayer or multicell spheroids. For exponentially growing cells, adriamycin cytotoxicity increased with increased dosage up to 2.5 $\mu$g/ml, and this cytotoxicity was reduced when the cells were grown in a plateau phase or in an acidic microenvironment (pH 6.6). This reduced cytotoxicity was correlated with the uptake of the drug. For multicell spheroids, the cytotoxicity of the drug was reduced dramatically, and this reduction was also correlated with a reduced uptake of the drug and an acidic pH inside of the spheroids. When the drug cytotoxicity was evaluated at different locations within the spheroids, the cells in the inner regions were least affected by the drug, suggesting that both an acidic microenvironment and noncycling plateau phase cells are contributing factors in decreasing the efficacy of the drug in an organized tissue, such as multicell spheroids.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1986년도 추계학술대회
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• pp.527.3-527
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• 1986

Several factors predicted to affect the protoplast formation and regeneration were investigated. The optimum lysozyme, casamino acid and PVP concentration were 0.5 (mg/$m\ell$), 0.1 (%) and 1.5(%). In B. pumilus, Penicillin-G treatment concentration was 0.3 (U/$m\ell$) and optimum treatment period was transit log. phase. And in the case of Celm. fimi, 0.3 (U/$m\ell$) and initial log. phase. Osmotic stabilizer and di-cation for OSM medium of B.pumilus and Gelm .fimi were 25mM CaCl2, 0.5M sodium sucinate and 50mM MgCl$_2$, 100mM CaCl$_2$, 0.4M sodium succinate. The regeneration frequency of B.pumilus and Celm. fimi were 14.6(%) and 6.9(%).

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2005년도 학술발표회 논문집
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• pp.47-51
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• 2005

If a structure is founded on the ground saturated with pore water, then the ground should be modeled as a saturated two-phase porous medium for accurate earthquake response analysis. In this study, an axisymmetric transmitting boundary hyperelement is developed for modeling of far field of the ground using u-U formulation for water-saturated transversely isotropic layered stratum. The developed hyperelement is verified by comparing the dynamic stiffness of rigid circular foundation on water-saturated isotropic layered stratum with the case of using equivalent single-phase medium model.