• Title/Summary/Keyword: U-band

Search Result 536, Processing Time 0.024 seconds

Effects of pH on Purification of GFPuv/Cytochrome c-552 Fusion Protein

  • Lee, Sang-On;Hong, Eul-Jae;Choe, Jeong-U;Hong, Eok-Gi
    • 한국생물공학회:학술대회논문집
    • /
    • 2003.04a
    • /
    • pp.539-542
    • /
    • 2003
  • Fusion gene of GFPuv and Cytochrome c-552 was inserted into the pTrcHis B vector and transferred to E. coli. A fusion protein of GFPuv and Cytochrome c-552 was expressed in BL21. This fusion protein was composed of a His-tag for purification using an immobilized metal affinity chromatography(IMAC). IMAC constitutes a rather facile means of unravelling the principles of recognition and, in particular, of identifying the counterligands on the protein surface, which interact with the ligated and immobilized metal ions. Histidine when present on the surface of a protein molecule under a favorable solvent condition, may serve as electron donors in coordination with the immobilized chelates of some transition metal ions$(Ni^{2+})$.

  • PDF

Development of S-band Waveguide Valve for PLS 2-GeV Linac (PLS 2-GeV 선형가속기를 위한 S-band용 도파관 밸브의 개발)

  • 박주식;남궁원
    • Journal of the Korean Vacuum Society
    • /
    • v.4 no.2
    • /
    • pp.124-134
    • /
    • 1995
  • 포항가속기연구소에서는 PLS 2-GeV 선형가속기 클라이스트론의 교체 및 보수시에 사용하기 위하여, 80 MW급 S-band의 도파관 밸브를 개발중에 있다. PLS형 도파관 밸브는 결합수단 없이 대전력을 전송할 수 있는 구조로 설계되어, 전력전송에 대한 제한을 받지 않는 특징을 갖는다. 본 도파관 밸브의 주요구성부는 밀착구동체를 갖는 U자형 도파관부, 두 개의 H-코너가 부착된 진공함, 그리고 두 개의 Viton O-링이 장착된 진공기밀판으로 이루어져 있다. 시제품용 도파관 밸브의 특성조사 결과, 펄스폭 3.5 $\mu$sec, 반복율 30 Hz에서 65MW의 전력전송을 얻을 수 있었다. 본 논문에서는 PLS도파관 밸브의 설계 개념, 기계적 특성 및 가공의 순으로 자세히 논할 것이며, 또한 현재까지의 실험결과 및 앞으로의 계획에 대하여 보고하기로 한다.

  • PDF

The Design of 2.4GHz Band LTCC Bandpass Filter using $\lambda/4$ Hairpin Resonators ($\lambda/4$ Hairpin 공진기를 이용한 2.4GHz 대역 LTCC 대역통과 여파기의 설계)

  • Seong Gyu Je;Choe Jae U;Park Hyeon Sik;Park Jang Hwan;Yeo Dong Hun
    • Proceedings of the International Microelectronics And Packaging Society Conference
    • /
    • 2003.11a
    • /
    • pp.260-264
    • /
    • 2003
  • In this paper, a $\lambda/4$ hairpin resonator is proposed to reduce the size of planar resonators for a LTCC MLC bandpass filter. The $\lambda/4$ hairpin resonator operates as stepped impedance resonator (SIR) without changing the width of the planar resonator. It is composed of two sections those are parallel coupled line and transmission line. The characteristic impedance of two sections is different each other. The design formulas of the bandpass filter using the coupling element at the arbitrary position are derived from even and odd-mode analysis. The formulas can take account of the arbitrary coupling of lumped ana/or distributed resonators. The advantage of this filter is its abilities to change freely the coupling structure between two resonators. Experimental bandpass filters for 2.4GHz Band are implemented and their performances are shown.

  • PDF

Improvement of a sensor unit for wrist blood pressure monitor (손목형 혈압계의 센서부 개선에 대한 연구)

  • Koo, Sang-Jun;Kwon, Jang-Wan;Park, Yong-Man;Ayuznara, Odgerel;Kim, Hie-Sik
    • Proceedings of the KIEE Conference
    • /
    • 2007.04a
    • /
    • pp.380-382
    • /
    • 2007
  • As the society changes more to the aging society in future, many healthcare product are developed and distributed more on the market. The digital wrist band tye blood pressure device for home use are popular already in the market. It is useful for checking blood pressure level at home and control of hypertension. Especially. It is very essential home device to check the health condition of blood circulation disease. Nowadays many product types are available. But the measurement accuracy of blood pressure is not enough compared to the mechanical type. It needs to be upgraded to assure the precise health data enough to use in the hospital. The structure, feature and output signal of capacitor type pressure sensors are analyzed. An improved design fa capacitor sensor is suggested. It shows more precise health data after use on a wrist band type health unit. They can be applied for remote u-health medical service.

  • PDF

무궁화 3호 위성 현황 및 탑재체 성능분석

  • Jang, Sang-Gu;Park, Jeong-U;Kim, Seong-Jung
    • Satellite Communications and Space Industry
    • /
    • v.7 no.2 s.16
    • /
    • pp.34-46
    • /
    • 1999
  • 한국통신은 현재 동경 116도 궤도에 무궁화1호 및 2호를 운영하며 통신 및 방송 서비스를 제공하고 있다. 무궁화 3호 위성은 대용량 위성으로 1/2호 위성의 중계기 용량을 모두 수용하며 동남아 지역을 대상으로 하는 가변빔 및 Ka-band 중계기를 탑재하고 있다. 3호 위성은 발사 후 동경 116도에 위치하여 올해로 수명이 다하는 1호 위성이 제공하는 서비스 및 2호 위성 서비스를 수용할 예정이며, 2호 위성은 113도로 이동하여 신규 서비스를 수용할 예정이다. 본 고에서는 무궁화3호 위성 탑재체의 설계사양 및 설계단계 성능분석에 대해 간략하게 소개하며, 분석결과와 실제 측정 데이터를 비교하였다. 또한 Ka-band 중계기의 주요 성능 파라미터들의 변화에 따라 디지털 통신캐리어를 가정하여 무궁화3호 위성 통신시스템의 비트오율 성능분석을 실시하였다. 시뮬레이션을 실시한 이유는 탑재체에 실제 서비스 캐리어가 실린 경우 탑재체의 통신품질을 예측하기 위함이다. 무궁화3호 위성의 공정현황 및 제공서비스에 대해서도 간략하게 소개하였다.

  • PDF

A Study on TMJ Sound by Sonopak (SONOPAK를 이용한 약관절음에 관한 연구)

  • Sung-Chang Chung;Soo-Young Kim
    • Journal of Oral Medicine and Pain
    • /
    • v.17 no.1
    • /
    • pp.9-17
    • /
    • 1992
  • The purpose of this study was to investigate the characteristics of TMJ sounds by computerized sound power-spectrum analyser(SONOPAK, Bioresearch Inc., U.S.A.). TMJ sounds were recorded and anaylsed in the 87 patients wit TMJ noises by SONOPAK. The followings are the criteria of TMJ sound analysis. 1. It is possible to record the location of the TMJ sound in relation to the opening/closing cycle of mouth. 2. It is possible to record amplitude of sound(loudness) and frequency of sound (Hz) 3. Clicks display a narrow band of sound within the 0-300 Hz range. The peak frequency generally occurs between 50-150 Hz. And crepitus appear as a wide band of sound, occuring from 0-1300Hz. The data obtained from quantitative TMJ sound analysis give lots of information, but further researches are needed.

  • PDF

Improving the Algorithm of a Diffusion Filter U sing a Difference Network and Quantitative Analysis of Band Pass Characteristics (차분망을 이용한 확산필터 알고리즘의 개선 및 대역통과특성의 정량적 분석)

  • 허만택;남기곤;김재창;이종혁;김길중;윤태훈;박의열
    • Journal of the Korean Institute of Telematics and Electronics B
    • /
    • v.33B no.7
    • /
    • pp.163-172
    • /
    • 1996
  • Recently, it was reported that gaussian distribution and difference of two gaussians (DOG) to have band pass characteristics can be generated by simple iterative processes of the diffusion networks. In this paper, we propose method of improved implementation of a diffusion filter which can reduce total runing time, and operate by simple algorithm in contrast to the latest diffusion filter. We rebuild the diffusion network to a difference network which can generate DOG independently. Different filter characteristics are obtained just by each diffusion process and difference process. Quantitative analysis shows that the center frequency and the selectivity of each filter channel can be varied independently. Also, it would requires smaller amount of hardwares than conventioanl method to build a filter bank.

  • PDF

Filter wheel design for CQUEAN II

  • Kim, Sanghyuk;Pak, Soojong;Lee, Hye-In;Im, Myunshin;Shin, Sang-Kyo
    • The Bulletin of The Korean Astronomical Society
    • /
    • v.39 no.1
    • /
    • pp.51.2-51.2
    • /
    • 2014
  • CQUEAN(Camera for QUasars in EArly uNiverse) has been used at the 2.1 m Otto Struve Telescope of the McDonald Observatory since 2010. This camera is optimized at 0.7 - 1.1 um for the survey of Lyman break of high redshift (z > 5) quasars in the early universe. The current system has a filter wheel consist of seven (g', r', I', z', Y, Iz and Is) broad-band filters. We are upgrading this filter wheel to have 20 narrow band filters, with which we can take spectral energy distributions of targets. The new filter wheel consists of interchangeable cartridges for 50 mm square filters, a speed reducer unit, and a step motor. This new design of the large size filter wheel can be applied to other large format CCD cameras.

  • PDF

An Extended $H\ddot{u}ckel$ Study of Two Dimensional Layered Compound: FeOCl

  • Kim, Sang Ho;Kim Hojing
    • Bulletin of the Korean Chemical Society
    • /
    • v.14 no.1
    • /
    • pp.132-137
    • /
    • 1993
  • The electronic structure of two dimensional layered compound, FeOCl, is studied with the band model and the cluster model approximation employing Extended-Huckel (EH) method. We examine the effects of intercalation (e.g., localization of transferred electron, conductivity increase). FeOCl has the electronic structure typical for layered compounds as expected. For FeOCl-$Li_{1/2}$ system, the charge transfer from Li to the FeOCl lattice occurs, and electrons are built up almost exclusively on Fe atoms. The partially filled band of FeOCl-$Li_{1/2}$ complex is responsible for the increase in conductivity.

Ultrastructural Characteristics of Necrosis and Stunt Disease in Red Pepper by the Mixed Infections of Tobacco mosaic virus-U1 or Pepper mild mottle virus and Pepper mottle virus

  • Kim, Dae-Hyun;Cho, Jeom-Deog;Kim, Jae-Hyun;Kim, Jenog-Soo;Cho, Eui-Kyoo
    • The Plant Pathology Journal
    • /
    • v.21 no.3
    • /
    • pp.252-257
    • /
    • 2005
  • In single infection of Tobacco mosaic virus-U1 (TMV­U1) or Pepper mild mottle virus (PMMoV), mosaic symptoms were produced on the chili pepper cultivars of 'Cheongyang' and 'Wangshilgun'. However, in cultivars of 'Manitta' and 'Bugang', no symptoms were occurred. In single infection of Pepper mottle virus (PepMoV), symptoms of mottle and malformation were produced on the tested cultivars of 'Manitta', 'Bugang', 'Cheongyang', and 'Wangshilgun'. In the cultivars of 'Cheongyang' and 'Wangshilgun', synergistic symptoms of stunt and lethal death were induced by mixed infections in the two combinations of TMV-U1 + PepMoV and PMMoV+PepMoV. However, in cultivars of 'Manitta' and 'Bugang', synergistic symptoms were not noted, but mottling which was milder than that of single infection was produced. Cells infected singly with TMV-U1 and PMMoV in the cultivars of 'Cheongyang' and 'Wangshilgun', respectively, had the typical ultra-structures of tobamovirus as the stacked-band structure and multiple spiral aggregate (SA). In the cells and tissues infected with PepMoV on the cultivars of 'Cheongyang', 'Wangshilgun', 'Manitta' and 'Bugang', the potyvirus inclusions of pinwheels, scrolls, lamminated aggregates and amorphous inclusion were observed. In the cells infected mixedly with combinations of TMV­U1+PepMoV and PMMoV+PepMoV, the virus particles and inclusions of the two different viruses were found simultaneously in the same cytoplasm. The amounts of virus particles in mixed infections were more abundant than in single infection. The angled-layer aggregates (ALA) were observed only in the cells infected with both TMV-U1 and PepMoV.