• 한국잠사곤충학회지
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• 제50권2호
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• pp.109-111
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• 2012

성공적인 생물학적 방제를 위해서는 적용천적의 사육기준과 규격이 설정되어 있어야 한다. 8, $12^{\circ}C$, 습도 $70{\pm}5%$, 24시간 암조건에서 총채가시응애와 그 먹이응애인 긴털가루응애의 저온 저장 실험을 수행하였다. 긴털가루응애는 소맥피(50%)와 쌀겨(50%)로 조성된 먹이사료에 접종하여 $12^{\circ}C$에서 저온저장이 가능하였다. 긴털가루응애와 총채가시응애의 생존율이 70%되는 조건은 $12^{\circ}C$에서 28일, 70일 저장후이다. 총채가시응애를 $12^{\circ}C$에서 28, 56, 84일 저온 저장후 증식율을 확인한 결과 저온기간에 상관없이 유사한 결과를 나타내었다.

• Journal of Applied Biological Chemistry
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• 제58권2호
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• pp.101-104
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• 2015

긴털가루응애 성충을 청색(470 nm), 녹색(520 nm), 황색(590 nm)과 적색(625 nm) light-emitting diode (LED)에서 파장별 광량을 20, 40 및 60 lx로 달리하여 주광성 행동반응 판별장치(LED-equipped Y-maze chamber)에서 상업적 양성대조구 BLB와 유인활성을 비교하였다. 긴털가루응애는 사용된 청색, 녹색, 황색 및 적색 LED 파장과 20, 40 및 60 lx 광량에서 특별한 행동반응특성을 발견하지 못하였으며, 양성대조구로 이용된 BLB에서도 긴털가루응애는 음성/양성주광성 반응을 보이지 않았다. 이러한 결과를 근거로 긴털가루응애는 LED 주광성 행동반응 판별장치에서는 친환경적 해충방제 가능성을 제시하지 못했다.

• Journal of Applied Biological Chemistry
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• 제58권2호
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• pp.105-108
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• 2015

This research is aimed at evaluating the potential abilities of the natural acaricide of F. suspense oil against Tyrophagus putrescentiae and Dermatophagoides spp. Based on the $LD_{50}$ values, in contact bioassay, F. suspense oil (8.19, 3.28, and $4.35{\mu}g/cm^2$) showed acaricidal effects against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively. Fumigant toxicities of F. suspense oil showed similar patterns as those observed with contact toxicities. GC/MS analysis showed the major components of F. suspense oil to be ${\beta}$-pinene (45.88%), myrtenol (13.86%), (+)-${\alpha}$-pinene (13.09%), (-)-trans-pinocarveol (7.34%), sabinene (6.64%) and pinocarvone (4.13%). These findings indicate that F. suspense oil has potential as a natural acaricide.

• Journal of Applied Biological Chemistry
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• 제58권3호
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• pp.197-199
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• 2015

The composition of the essential oil of Ligustrum japonicum leaves was determined by GC-MS analysis. The major constituents of L. japonicum leaf oil were germacrene D (40.50%), ${\alpha}$-pinene (13.63%), (-)-${\beta}$-elemene (6.42%), ${\beta}$-caryophyllene (5.73%), and $\delta$-cadinene (5.47%). The acaricidal activities of L. japonicum oil were evaluated against acaridae and pyroglyphid mites. In the fumigant bioassay, the $LD_{50}$ values of L. japonicum oil were 16.48, 12.38, and $15.63{\mu}g/cm^3$ against Tyrophagus putrescentiae, Dermatophagoides farinae, and D. pteronyssinus, respectively. In the contact bioassay, the $LD_{50}$ values of L. japonicum oil were 8.02, 5.02, and $7.67{\mu}g/cm^2$ against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively.

• Parasites, Hosts and Diseases
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• 제62권3호
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• pp.365-377
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• 2024

Exposure to storage mite (SM) and house dust mite (HDM) allergens is a risk factor for sensitization and asthma development; however, the related immune responses and their pathology have not been fully investigated. The HDMs Dermatophagoides farinae and Dermatophagoides pteronyssinus and SM Tyrophagus putrescentiae are potent allergens that induce asthma. Most SM-related studies have focused on the allergic reactions of individuals by measuring their immunoglobulin (Ig)E expression. Considering the limited research on this topic, the present study aims to investigate the differences in the immune responses induced by HDMs and SMs and histologically analyze lung tissues in a mouse asthma model to understand the differential effects of HDM and SM. The results revealed that all mite species induced airway inflammation. Mice challenged with T. putrescentiae had the highest airway resistance and total cell, eosinophil, and neutrophil counts in the bronchoalveolar lavage fluid (BALF). The SM-sensitized groups showed more severe lesions and mucus hypersecretions than the HDM-sensitized groups. Although the degree of HDM and SM exposure was the same, the damage to the respiratory lung tissue was more severe in SM-exposed mice, which resulted in excessive mucin secretion and increased fibrosis. Furthermore, these findings suggest that SM sensitization induces a more significant hypersensitivity response in mucosal immunity than HDM sensitization in asthma models.

• Parasites, Hosts and Diseases
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• 제48권2호
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• pp.151-155
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• 2010

Allergen extracts from dust mites and cockroaches commonly found in Korean homes were used to evaluate their enzymatic activity as they are believed to influence allergenicity. Allergen extracts were prepared from 3 dust mite species (Dermatophagoides farinae, D. pteronyssinus, and Tyrophagus putrescentiae) and 3 cockroach species (Blattella germanica, Periplaneta americana, and P. fuliginosa) maintained in the Korea National Arthropods of Medical Importance Resource Bank. Proteins were extracted in PBS after homogenization using liquid nitrogen. The activities of various enzymes were investigated using the API Zym system. No significant difference in phosphatase, lipase, or glycosidase activity was observed among the 6 allergen extracts, but much difference was observed in protease activity. Protease activity was assessed in more detail by gelatin zymography and the EnzChek assay. Extract from T. putrescentiae showed the highest protease activity, followed by those of the cockroach extracts. Extracts from D. farinae and D. pteronyssinus showed only weak protease activity. Gelatinolytic activity was detected mainly in a 30-kDa protein in D. farinae, a 28-kDa protein in D. pteronyssinus, a > 26-kDa protein in T. putrescentiae, a > 20-kDa protein in B. germanica, and a > 23-kDa protein in P. americana and P. fuliginosa. The information on various enzymatic activities obtained in this study may be useful for future studies. In particular, the strong protease activity found in cockroach extracts could contribute to sensitization to cockroach allergens, which is known to be associated with the development of asthma.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2001년도 추계 합동 심포지엄 및 학술발표대회
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• pp.151-151
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• 2001

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.528-529
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• 2005

• Food Science and Biotechnology
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• 제14권1호
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• pp.73-76
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• 2005

The acaricidal activities of 12 commercial constituents derived from Eugenia caryophyllata leaf oils against Dermatophagoides farinae, D. pteronyssinus and Tyrophagus putrescentiae adults were examined using an impregnated fabric disk application and compared with that of the commercial benzyl benzoate as synthetic acaricide. On the basis of $LD_{50}$ values, the most toxic compound was methyl eugenol ($4.13\;{\mu}g/cm^2$), followed by methyl isoeugenol ($4.19\;{\mu}g/cm^2$), isoeugenol ($4.29\;{\mu}g/cm^2$), eugenol ($4.94\;{\mu}g/cm^2$), and acetyl eugenol ($13.91\;{\mu}g/cm^2$) against D. farinae. In the case of D. pteronyssinus, isoeugenol ($2.93\;{\mu}g/cm^2$) was the most toxic, followed by methyl isoeugenol ($3.28\;{\mu}g/cm^2$), methyl eugenol ($3.87\;{\mu}g/cm^2$), eugenol ($3.92\;{\mu}g/cm^2$), and acetyl eugenol ($7.21\;{\mu}g/cm^2$). These results suggest that D. pteronyssinus may be controlled more effectively by the application of eugenol congeners than D. farinae. In comparison with synthetic acaricides, the acaricidal activities of eugenol, isoeugenol, methyl eugenol, and methyl isoeugenol were about 1.9-2.2 times more toxic than benzyl benzoate. Furthermore, the most toxic constituent against T. putrescentiae was exhibited on eugenol ($10.11\;{\mu}g/cm^2$), followed by methyl eugenol ($38.67\;{\mu}g/cm^2$) and acetyl eugenol ($70.09\;{\mu}g/cm^2$), but no activity was observed for isoeugenol and methyl isoeugenol. The results suggested that eugenol congeners may be useful as a new source for selective control of house dust mites and stored food mites.

• Journal of Applied Biological Chemistry
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• 제59권2호
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• pp.125-128
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• 2016

The chemical compositions of the essential oil extracted from Albizziae julibrissin barks were analyzed by Gas chromatography-Mass spectrometry spectrometry. Fourteen components were identified, representing 89.23 % of the total oil composition. The analysis of the essential oil revealed that the essential oil contains 14 compounds, accounting for 89.23 % of the total oil. Hexanoic acid was the principal component (41.43 %) of the essential oil, followed by 4,4,6-trimethyl-cyclohex-2-en-1-ol (11.16 %), palmitic acid (9.00 %), 2-pentylfuran (5.66 %), 2-butyl-2-octenal (4.12 %), linoleic acid (3.10%), amyl hexanoate (3.01%), (E,E)-2,4-decadienal (2.49 %), 2-hexylthiophene (2.47 %), caprylic acid (2.13 %), ${\delta}-undecalactone$ (1.52 %), heptanoic acid (1.27 %), 3,5-octadien-2-ol (0.99 %), and 2-octenal (0.88 %). The acaricidal activity of the A. julibrissin oil was tested against Dermatophagoides farina, D. pteronyssinus and Tyrophagus putrescentiae by the fumigant bioassay. Based on the $LD_{50}$ values, the essential oil exhibited strong acaricidal activities against D. farinae ($LD_{50}$, $4.88{\mu}g/cm^3$), D. pteronyssinus ($2.44{\mu}g/cm^3$), and T. putrescentiae ($1.22{\mu}g/cm^3$). These results indicate that A. julibrissin oil could be a source of acaricidal agents for mite control.