• 한국식물병리학회지
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• 제14권6호
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• pp.622-624
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• 1998

During the course of study on plant parasitic nematodes associated with medicinal herbs in Korea, high population of different stages of citrus nematode, Tylenchulus semipentrans was found in association with Pyrola japonia Klenze in Imsil, Chunbuk province. The second stage larvae and males were isolated from rhizosphere soil ; immature and mature females were detached from the roots of infested plants of Pyrola japonica. Besides citrus it has been reported to parasitize on roots of Syringa vulgaris, Diospyros lotus, olive, grapevine, and pear in different parts of the world. This is the first record of T. semipenetrans parasitized on a medicinal plant, Pyrola japonica.

• The Plant Pathology Journal
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• 제25권3호
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• pp.236-240
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• 2009

Tylenchulus semipenetrans, citrus nematode is an important phytopathogenic nematode and responsible for serious damage on citrus. However, little information is available about genetic variability of T. semipenetrans among different populations with variation of conventional diagnostic characteristics. In this study, we compared the morphometric and genetic characteristics among different populations. The mature female of T. semipenetrans collected in this study had thicker cuticle than those in the previous studies. In comparative sequence analysis of T. semipenetrans populations obtained from Jeju in Korea, we observed genetic variations within clones generated from single individuals. To determine whether variability among copies of nuclear ribosomal DNA sequences exists in the genome of T. semipenetrans, PCR-RFLP technique from individuals of Korean isolates with MseI and MspI restriction enzymes was used to prove experimentally that all populations have intra-specific variations. Restriction enzyme digestion created several fragments on 3.0% agarose gel corresponding to several haplotypes in all populations, though some populations displayed fragment deletion. The total length of fragments was larger than before digestion, indicating sequence heterogeneity within the genome of T. semipenetrans.

• The Plant Pathology Journal
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• 제33권2호
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• pp.184-192
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• 2017

Tylenchulus semipenetrans is an important and widespread plant-parasitic nematode of citrus worldwide and can cause citrus slow decline disease leading to significant reduction in tree growth and yield. Rapid and accurate detection of T. semipenetrans in soil is important for the disease forecasting and management. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed to detect T. semipenetrans using DNA extracted from soil. A set of five primers was designed from the internal transcribed spacer region (ITS1) of rDNA, and was highly specific to T. semipenetrans. The LAMP reaction was performed at $63^{\circ}C$ for 60 min. The LAMP product was visualized directly in one reaction tube by adding SYBR Green I. The detection limit of the LAMP assay was $10^{-2}J2/0.5g$ of soil, which was 10 times more sensitive than conventional PCR ($10^{-1}J2/0.5g$ of soil). Examination of 24 field soil samples revealed that the LAMP assay was applicable to a range of soils infested naturally with T. semipenetrans, and the total assay time was less than 2.5 h. These results indicated that the developed LAMP assay is a simple, rapid, sensitive, specific and accurate technique for detection of T. semipenetrans in field soil, and contributes to the effective management of citrus slow decline disease.

• 한국응용곤충학회지
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• 제14권3호
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• pp.141-146
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• 1975

감교선출(Tylenchulus semipenetrans)에 대한 DBCP(Dibromo chloropropane) $80\%$ 유제의 처리방법 및 약량과 회수를 규명코저 시험하여 아래와 같은 결과를 얻었다. 1. 약량의 증가에 다라, 점주가 관주처리보다 좋은 효과를 보였다. 2. 온주귤원에서는 배수가 나빠서 2회처리로서 좋은 효과를 볼 수 있었다. 3. 점토함량이 낮은 하귤원에는 3.3-4.4l/10a의 1회점주처리가, 점토함량이 높은 온주귤원에는 4.4l/10a씩 2회점주처리가 좋았다.

• 한국응용곤충학회지
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• 제48권4호
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• pp.535-539
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• 2009

이 연구는 Citrus속 과수재배지에서 식물기생선충의 종류와 분포를 조사하기 위해 수행하였다. 토양 샘플은 주요 재배지에서 2008년 4월부터 8월에 채집하였다. 식물기생선충은 전체 178개 샘플 가운데 139개 샘플에서 검출되었다. 감귤재배지에서 Tylenchulus semipenetrans가 178토양샘플 중 139 샘플에서 검출되었고(90.4%), 유자재배지는 94 토양샘플 중 85토양 샘플에서 T. semipenetrans가 검출되었다(71.4%). 그러나 주요한 식물기생선충의 종류는 다소 차이가 있었다. 국내 Citrus속 과수 재배지에는 T. semipenetrans가 널리 분포하고 있었으며, 감귤과수에 가장 중요한 식물기생선충임을 확인하였다. 재배 년수가 증가할수록 T. semipenetrans의 검출빈도가 증가하였다. 또 토양산도가 알칼리성으로 갈수록 감귤선충의 밀도는 증가하였으나, 상관계수는 높지 않았다.